층수돌기 방선균증 : 1예 보고

이범하,이혜경,박성진,차장규,김희경 순천향대학교 의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2S

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당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.

건설현장 근로자들의 안저예방실태와 사고예방과의 개선요인 조사연구

심규범,남철현,최상복,이송권,이정희,이순자,문기내,김문환 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

This study was conducted to examine aspects of health and safety education and demand level of safety education in construction job sites in order to provide direction of safety education development. The subjects of this study were 566 workers who were working in construction job sites located in Daegu City and Kyungbuk Province. Data were collected from June 1, 2002 to August 30, 2002. The results of this study can be summarized as follows. 1. According to safety education by experience methods, the proportion of education by lecture was highest (90.6%). The proportion of education by field practice was 13.1%. 2. Among the education methods which they thought as the most desirable method, the proportion of audio-visual education was 39.4% and the proportion of education by experience was 37.5%. Those who had more monthly income and higher education level preferred audio-visual education, while those who were younger and less educated preferred education by experience. 3. In the case of the contents of the safety education which they thought as the most important one, the proportion of enhancement of safety awareness and education of risk factors was highest (24.7%). The groups of older workers, having longer working period, and belonging to higher education level preferred these contents. 4. According to the factors which were most important to increase effectiveness of the safety education were workers' voluntary participation (27.7%), 40 to 49 years old (27.7%), and monthly income of one million, five hundred thousand won to one million, nine hundred ninety thousand won (32.0%). The proportions of lower educated workers and working as daily workers were also high. 5. The variable which most influenced the safety education was age. The safety awareness of construction job sites was the second highest influential variable. From above results, it is clear that the workers' participation in the safety education was low since most of the safety educations were formal education by lecture. Although they participated in the education, the education was also not effective because they compulsorily participated in it. Therefore, it can be concluded that effective alternative policies must be formulated.

동양란의 미세대량증식을 위한 효율적 배지조성

김범규,정규화 麗水水産大學校産業技術硏究所 1997 産業基術硏究所 論文集 Vol.6 No.-

Experiments are described which determine the optimal nutrient medium for in vitro micropropagation of oriental Cymbidium. Actively growing one year-old rhizomes were cultured for five months in 15 different formulated media, and their responses were investigated. The hyponex(N:P:K=6.5:6:19) basal medium supplemented with 4g tryptone, 1.5g activated charcoal, and growth regulators combinated with 1mg NAA+1mg kinetin or 2mg NAA+0.5∼1mg BA per liter was found to be suitable for rhizome growth and organogenesis. Several media factors affecting in vitro culture of rhizomes were also discussed.

핵산의 절단을 유발하는 전이금속착화합물의 합성

김희정,이범규,고문주 조선대학교 환경연구소 2000 環境硏究 Vol.16 No.1

Study on the synthesis of chemical nucleases that have ability of cleavage of RNA or DNA to investigate the structure of nucleic acids was performed. We synthesized Ni-complex that was synthesized by Karn and Busch (1966) and identified the specificity and ability as chemical nuclease by Chen et. al. (1993), and Cu-complex and Cd-complex that were presumed as having specificity similar to Ni-complex in terms of the structure and specificity and ability as chemical nuclease. The spectra of synthesized new transition metal complexes were compared with those of Ni-complex. Results of comparing data of UV and IR spectra of Cu-complex and Cd-complex with those of Ni-complex showed that the structures of newly synthesized transition metal complexes were similar to the structure of Ni-complex. The yield of synthesis of Ni-complex, Cu-complex, and Cd-complex was 81%, 7%, and 39% respectively.

Graphene: Position‐ and Morphology‐Controlled ZnO Nanostructures Grown on Graphene Layers (Adv. Mater. 41/2012)

Kim, Yong‐,Jin,Yoo, Hyobin,Lee, Chul‐,Ho,Park, Jun Beom,Baek, Hyeonjun,Kim, Miyoung,Yi, Gyu‐,Chul WILEY‐VCH Verlag 2012 Advanced Materials Vol.24 No.41

<P>On page 5565, Gyu‐Chul Yi and co‐workers grow position‐ and morphology‐controlled ZnO nanowalls in prescribed positions on graphene layers. The nanowalls are grown to produce a variety of shapes from simple circles to text at the microscale. The selective growth of high quality ZnO nanowalls is investigated by electron microscopy and optical spectroscopy. The hybrid nanostructure can be exploited to fabricate various nanodevices including microarrays of nanotube LEDs. </P>

핵산의 절단을 유발하는 전이금속착화합물의 합성

김희정,이범규,고문주 조선대학교 환경공해연구소 2000 環境公害硏究 Vol.16 No.1

Study on the synthesis of chemical nucleases that have ability of cleavage of RNA or DNA to investigate the structure of nucleic acids was performed. We synthesized Ni-complex that was synthesized by Karn and Busch (1966) and identified the specificity and ability as chemical nuclease by Chen. et. al. (1993), and Cu-complex and Cd-complex that were presumed as having specificity similar to Ni-complex in terms of the structure and specificity and ability as chemical nuclease. The spectra of synthesized new transition metal complexes were compared with those of Ni-complex. Results of comparing data of UV and IR spectra of Cu-complex and Cd-complex with those of Ni-complex showed that the structures of newly synthesized transition metal complexes were similar to the structure of Ni-complex. The yield of synthesis of Ni-complex, Cu-complex, and Cd-complex was 81%, 7%, and 39% respectively.

GC/MS-SIM과 ³²P-postlabeling방법에 의한 염료와안료를 제조하는 사업장 근로자들의 방광암 조기발견을 위한 Biomarker개발에 관한 연구

이진선,신호상,김양호,노재훈,이범규 공주대학교 자원재활용신소재지역협력센터 2000 2차년도 센터 사업 성과집 Vol.2000 No.1

To develop the biomarker of non-invasive biomonitoring among workers who exposed benzidine-based dyes in its manufacturing and treating industries, we examined and evaluated the specific carciaogen-DNA adducts in exfoliated urothelial cells of 33 exposed workers. Samples were collected from urine of workers, and then isolated DNA from them. DNA adducts were analyzed using 32P-postlabeling, along with Nuclease P1. One adducts was predominated in those samples with 6 adducts present. It was formed in all exposed workers, but the others not. Its relative adducts level(RAL) was 73.2×107, which was statistically significantly higher than any other adducts' level(p<0.05). Analyzing regression between its RAL and employment period of workers, simple linear regression was positively increased with slop of 6.922(p<0.05, r2=0.548). And also, DNA adducts were analyzed using GC/MS-SIM, along with n-butanol extraction and trifluoroacetyl auhydride(TFA) derivatization. Standard material was synthetic N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. The characteristics of sample and standard were observed on the same sites, such as M+(m/z 376) and M+(m/z 279) for benzidine-di-TFA and mono-TFA. And then, they were confirmed by analyzing with secondary ion mass(SIM). This study showed that benzidine-based dyes was formed one DNA adduct into significantly high levels in bladder of all exposed workers, its chemical formula was N-(deoxyguanosin-8-yl)-N'-acetylbenzidine. So we can do monitoring the workers with exfoliated urothelial cells of them by analyzing above DNA adduct as biomarker for preventing bladder cancer causing the exposure of the benzidine-based dyes.

중·고등학생들의 보건교육 요구도 조사연구

문기내,남철현,김귀희,위광복,박경옥,이미경,심규범,장영진 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

This study was conducted to examine middleㆍhigh school 720 students in seven regions of middleㆍsmall city and town, Myon for 2 months from March 2 through April 30, 2002 in order to realize a side of school health education in middleㆍhigh school and health education need as well as health education specialist in school in the future. The results of this study can be summarized as follows. 1. Health condition is now 'normal' is 51.0%, 'good' is 45.0%, 'not good' is 4.1% and health condition of children is good as much as education level of mother is higher. 2. Experience to learn health education in school for one year recently is 'no' is 39.6%, 'don't know' is 32.6%, 'yes' is 27.8% and it represents health education in school doesn't work well and experience to learn health education is lower and lower in town and Myon. 3. Health education ways to work in school now are 'Movie, Video, Slide' is 40.7%, 'education as groups of nurse-teacher' is 38.6%, 'to distribute health education material and book' is 7.7%, 'education by setting an example' is 3.4%, 'the others' is 9.7%. 4. Time of appropriate health education for one time is 'about a hour' is 56.7%, 'about 30 minute' is 33.8%, 'about two hour' is 7.6%, it represents this situation is because of what students' time is lack and burden about a curriculum in an education administratin and school classed of a focused school score. 5. The average score of satisfaction of health education in school is 16.99±0.58, 'men' is 1.79±0.60, 'women' is 1.61±0.55 as the distinction of sex, satisfaction level of men is higher than women. 6. Learning route about health information is 'through mass media' is 59.9%. 'through family, friend' is 15.0% 'health education lecture' is 7.2%, 'printing media' is 6.1%, 'medical institution' is 5.6%, 'the others' is 5.1%, 'public health center' is 1.0%, 'through mass media' is the highest percent as each 59.1% and 60.6% in all of men and women. 7. The most efficient education way of health education is 'movie, video, slide' is 32.6%, 'health education specialist who works school' is 21.9%, 'school health education by nurse-teacher' is 16.0%, 'way through mass communication' is 11.6%. 8. The appropriate health education specialist in the future is 'health education specialist who has qualifications' is the highest as 64.5%, 'nurse-teacher' is 12.4%, 'medical personnel(doctor, a physician of oriental medicine etc.,)' is 11.0%, 'staff in public health center' is 7.2%, people in other regions(health and medical professor etc.,) is 7.0%, it represents school health education is not actual and is formal now in second hand. 9. The most necessary health education program is 'program of stress solution' is the highest as 24.9%, 'program of no smoking' is 22.7%, 'sexual education program' is 19.8%, 'school violence program' is 8.8%, 'isolate from their friends prevention program' is 8.0%, solution program of stress which gives student by school score was very necessary. Therefore, school health education is formal and inefficient because school education focuses on an entrance exam, so middleㆍhigh school students decrease concern and interest about health education. Furthermore, reliance is gotten down about nurse-teacher who takes charge of school health, a charged person of school health education wants more chargeable health education staff. And a solution program of stress that gives students by school score was very necessary. According to active concern and planned, systematic school health education is necessary to give right health knowledge, health maintenanceㆍpromotion and setting of appropriate health education specialist is very urgent.

처분장근처에서 침식에 의한 Sr 화학종의 평형

조윤정,김성현,이인화,김시욱,고문주,박성규,이범규 조선대학교 환경공해연구소 2000 環境公害硏究 Vol.16 No.1

Leaching and adsorption equilibria of Sr Chemical Species from the near field of a repository has been studied in solidified cement with Fe and oxidized Fe. The absorption percent, Ads %, and distribution coefficient, K_(d) were calculated using equilibrium concentration to predict the extent of leaching and adsorption. In the mixed aqueous solutions the Ads % increase as Cement(C)/Water(W) ratios increase and K_(d) was decreased for Sr. These behaviors were discussed by adsorption and equilibrium of spiked ions. Oxides Fe promoted the adsorption of spiked ions, but pure Fe had no effect. E_(h) values were also estimated and discussed to elucidate oxidation-reduction environment of experimental system.