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L1 Recombinant Proteins of HPV Tested for Antibody Forming Using Sera of HPV Quadrivalent Vaccine
Begum Akuzum,김시내,Tam Thanh Nguyen,홍재우,이시영,김은혜,김주희,최여옥,전현정,이영민,김현우,손동현,김수현 대한면역학회 2018 Immune Network Vol.18 No.3
Virus-like particles (VLPs) derived from human papillomavirus (HPV) L1 capsid proteins were used for HPV quadrivalent recombinant vaccine. The HPV quadrivalent vaccine is administrated in a 3-dose regimen of initial injection followed by subsequent doses at 2 and 6 months to prevent cervical cancer, vulvar, and vaginal cancers. The type 6, 11, 16, or 18 of HPV infection is associated with precancerous lesions and genital warts in adolescents and young women. The HPV vaccine is composed of viral L1 capsid proteins are produced in eukaryotic expression systems and purified in the form of VLPs. Four different the L1 protein of 3 different subtypes of HPV: HPV11, HPV16, and HPV18 were expressed in Escherichia coli divided into 2 fragments as N- and C-terminal of each protein in order to examine the efficacy of HPV vaccine. Vaccinated sera failed to recognize N-terminal L1 HPV type 16 and type 18 by western blot while they detected N-terminal L1 protein of HPV type 11. Moreover, the recombinant C-terminal L1 proteins of type 16 was non-specifically recognized by the secondary antibody conjugated with horseradish peroxidase. This expression and purification system may provide simple method to obtain robust recombinant L1 protein of HPV subtypes to improve biochemical analysis of antigens with immunized sera.
Begum Fatema Zohara,Azizunnesa,Md. Faruk Islam,Md. Golam Shahi Alam,Farida Yeasmin Bari 사단법인 한국동물생명공학회 2014 한국동물생명공학회지 Vol.29 No.2
A study was carried out on 16 indigenous ewes in Bangladesh in order to assess the reproductive physiology, thepattern of vaginal cell exfoliation and progesterone profiles during the estrous cycle period. The mean estrous cyclelength and duration of estrus were 15.8±0.12 days and 31.1±0.57 h respectively. The exfoliated epithelial cells werecategorized into parabasal, intermediate, superficial and keratinized and their relative occurrences. The percentages ofparabasal, intermediate and superficial cell type during proestrus were similar. The percentage of superficial cell typeduring estrus was 61.7%, which was significantly (p<0.01) differ from other types of cells and stages of estrus cycle. Metoestrus was predominant with neutrophils in addition with other cell types. Dioestrus was dominated byneutrophils. On days 0 to 5 of the cycle the progesterone concentration was 0.09 to 1.6±0.07 ng/ml. The length ofdiestrus was 5∼10 days with a range of mean progesterone level of 1.6±0.07 to 2.8±0.11 ng/ml. Progesterone levelsincreased significantly (p<0.01) after Day 5 and maximum level was 2.8±0.11 ng/ml observed on Day 10 of theestrous cycle. Thereafter it dropped rapidly to basal level of 0.11±0.04 ng/ml on Day 0 (p<0.01). These results indicatethat the pattern of exfoliation of vaginal cells along with progesterone concentration could be used to determine thereproductive stages of indigenous ewe
Begum, M.,Hossain, MM,Kim, IH INSTITUTE OF AGRICULTURAL FOOD INFORMATION 2014 VETERINARNI MEDICINA -PRAHA- Vol.59 No.9
<P>This study was aimed at evaluating the effects of different concentrations of the dietary plant extract supplement YGF251 (young growth factor) on growth performance, blood profiles, relative organ weight, nutrient digestibility and meat quality in broiler chickens. A total of 640 one-day-old male Arbor Acres broiler chickens with an average initial body weight of 40.25 ± 0.5 g were randomly allotted to one of four treatments lasting four weeks with 10 replicates per treatment and 16 chicks per replicate pen. Dietary treatments consisted of: CON (basal diet); YGF0.05 (CON + 0.05% YGF251); YGF0.1 (CON + 0.10% YGF251); YGF0.15 (CON + 0.15% YGF251). There were no statistical differences in body weight (BW), feed intake (FI) and feed conversion rate (FCR) throughout the whole experiment. However, broiler chickens fed with YGF0.1 diets had greater body weight gains (BWG) than chickens fed CON diets during eight to 28 days and zero to 28 days although the means of pH value, breast meat colour, WHC, drip loss, the relative organ weights of liver, spleen, bursa of Fabricius and breast muscle were not affected by any dietary supplementation (P > 0.05). The relative weight of abdominal fat in the CON treatment group was significantly higher than the YGF0.05 and YGF0.1 treatments, although relative gizzard weight was lower with CON treatment compared to YGF0.1 treatment (P < 0.05). Femur length and weight were significantly higher in the YGF251-supplemented chicks than in chicks fed the control diet (P < 0.05). Broiler chickens fed the YGF0.1 diet had significantly higher blood IgG counts compared to chicks fed the CON and YGF0.05 diets (P < 0.05). Moreover, YGF251-supplemented chicks exhibited increased IGF-I concentrations compared to the CON to YGF0.1 treatment (P < 0.05). The results of this study indicate that supplementation with 0.1% YGF251 can increase body weight, IgG and energy digestibility and reduce relative abdominal fat and gizzard weight in broiler chickens, while at all concentrations tested YGF251-supplemented chicks showed higher results for femur length and weight and serum IGF-I concentrations compared to the control treatment in broiler chickens. </P>
Begum, A.Naseema,Basavaraja, H.K.,Rekha, M.,Ahsan, M.M.,Datta, R.K. Korean Society of Sericultural Science 2001 International Journal of Industrial Entomology Vol.2 No.2
Screening of fifteen bivoltine silkworm breeds of Bombyx mori Linn at a temperature of $31{\pm}1^{\circ}C$ and relative humidity of 85${\pm}$5% resulted in the identification of eight thermo-tolerant breeds. The survival and cocoon shell ratio of the tolerant breeds ranged from 72.7 to 78.7% and 20.0 to 20.1% respectively. The tolerant breeds comprised of four oval breeds and four dumb-bell breeds. Eight foundation crosses prepared by crossing the oval and dumb-bell parents among themselves were screened at a temperature of $31{\pm}1^{\circ}C$ and relative humidity of 85{\pm}5%. The performance of the foundation crosses on 11 economic characters were analysed by employing Multipie Trait Evaluation Index method. Four foundation crosses which scored average index value > 50 were selected as breeding parents and breeding initiated for the evolution of thermo-tolerant bivoltine silkworm breeds. The methodology and the results of the foundation crosses reared both at 31{\pm}1$^{\circ}C$ and at $25{\pm}1^{\circ}C$ temperatures, are discussed.
Begum, Most Shirina,Jang, Inae,Lee, Jung-Min,Oh, Han Bin,Jin, Hyojin,Park, Ji-Hyung Elsevier 2019 The Science of the total environment Vol.676 No.-
<P><B>Abstract</B></P> <P>Dams and wastewater may greatly perturb riverine fluxes of dissolved organic matter (DOM) and CO<SUB>2</SUB>, yet little is known about the relationships between altered DOM quality and CO<SUB>2</SUB> emission in eutrophic impounded river systems. A basin-wide field survey of surface water CO<SUB>2</SUB> and dissolved organic carbon (DOC) was combined with laboratory incubations to examine how dams and urban tributaries delivering treated wastewater influence longitudinal patterns in DOM properties and CO<SUB>2</SUB> along the impounded Han River traversing Seoul metropolitan area. Fluorescent DOM indices including parallel factor analysis (PARAFAC) components were used to characterize DOM in relation to biodegradable DOC (BDOC). Compared with distinct downstream increases in DOC and CO<SUB>2</SUB>, BDOC concentration and its proportion in DOC (%BDOC) were highly variable along the mainstem and peaked at urban tributaries. Longitudinal increases in fluorescence index (FI), biological index (BIX), and two PARAFAC components (C2 and C3) contrasted with general decreases in humification index (HIX) and C1, reflecting increasing downstream inputs of anthropogenic DOM. During a 5-day incubation employing continuous CO<SUB>2</SUB> measurements, the cumulative production of CO<SUB>2</SUB> in the mainstem water mixed with urban tributary water was significantly higher than the level expected for conservative mixing of the two samples, indicating a synergistic enhancement of DOM biodegradation. Molecular formulas identified by Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS) revealed more consumed molecules in the mainstem water and more newly produced molecules in the tributary water over the 5-day incubation, implying abundant labile components in the mainstem water discharged from the upstream dam and highly processed tributary DOM limited in immediately biodegradable organic materials. Downstream increases in CO<SUB>2</SUB> and DOC along the Han River, combined with the synergistic effect observed in the mixed water, suggest that mixing wastewater-derived DOM with labile autochthonous DOM can enhance CO<SUB>2</SUB> production in the river system perturbed by impoundment and wastewater.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Field survey was combined with lab incubations to study human impact on river carbon. </LI> <LI> Dissolved organic matter and CO<SUB>2</SUB> vary along impounded river with wastewater influence. </LI> <LI> Urban tributary mixing enhances organic matter degradation and CO<SUB>2</SUB> in impounded river. </LI> <LI> FT-ICR-MS detects organic matter degradation in impounded river and urban tributary. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
CA 15-3 (Mucin-1) and Physiological Characteristics of Breast Cancer from Lahore, Pakistan
Begum, Mumtaz,Karim, Sajjad,Malik, Arif,Khurshid, Rukhshan,Asif, Muhammad,Salim, Asmat,Nagra, Saeed Ahmed,Zaheer, Ahmad,Iqbal, Zafar,Abuzenadah, Adel Mohammed,Alqahtani, Mohammed Hussain,Rasool, Mahmo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10
Background: High incidence of breast cancer and its fatal effect has reached an alarming stage across the globe, including the third world countries. Many factors have been reported to be associated with the development of breast cancer but detailed structural and functional information is missing. CA 15-3 is one of the known potential tumor marker of breast cancer; however little is known about structure and functional site of this protein. Present study aims to investigate the functional role of CA 15-3 in breast cancer, especially in development and metastasis. Material and Methods: Hundred female breast cancer patients confirmed by histopathological reports were included in the study. Their physiological characters were recorded in a performa. Enzyme linked immunosorbent assay (ELISA) technique was used to estimate serum CA 15-3 level. Immunohistochemistry was done for estrogen (ER), progesterone (PR) and Her2/neu receptors expression. Results: The study revealed the details of physiological characteristics of female breast cancer. Mean age was $37.72{\pm}5.99$ and $55.05{\pm}7.28$ years and serum CA 15-3 (MUC1) level was $60.47{\pm}8.59$ and $63.17{\pm}4.58$ U/ml in pre and post-menopause respectively, and both groups of women had sedentary life style. Their receptor status especially of progesterone, estrogen and HER-2/neu were positive in 50% of premenopausal women and 65% of postmenopausal women. Conclusion: There are multiple physiological factors promoting breast cancer. High serum CA 15-3 level and hormonal imbalance of ER, PR and Her2/neu appears to be the main cause of breast cancer. It may be possible that the functional sites of these proteins may be altered which may increase the chances of metastasis in breast cancer.
Begum, Halima,Ahmed, Mohammad Shamsuddin,Jeon, Seungwon American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.7
<P>A series of electrochemically deposited palladium nanotubes (e-PdNTs) on nanoclusters (e-PdNCs) mosaic basement have been prepared with variation of cycle number and scan rate through a facile electrochemical deposition method using cyclic voltammetry (CV) technique in 20 mM HCl solution and have been used as an anode electrocatalyst for hydrazine (N2H4) oxidation reaction. The morphology and structure of the self-formed e-PdNTs catalysts have characterized by scanning electron microscopy. As observed, the size and amount of e-PdNTs increased with the increasing of cycle number and decreased with respect to the increasing of scan rate. The overall experimental parameters have determined that the superior and direct electrocatalytic N2H4 oxidation obtained with lowering NH3 formation upon 6 cycles deposited e-PdNTs at 50 mV s(-1) scan rate in 0.1 M KOH solution than that of other prepared e-PdNTs and Pd/C in respect to the onset potential, current intensity and durability. The lower detection limit (3.6 mu M) and the linear range of N2H4 (up to 14 mM) have also been determined on e-PdNTs via amperometry for further sensor application.</P>
Effect of Aerva lanata against oxalate mediated free radical toxicity in urolithiasis
Begum, Vava Mohaideen Hazeena,Mahesh, Ramalingam,Ramesh, Thiyagarajan,Soundararajan, Periasamy Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.1
This study was undertaken to evaluate the antioxidant potential of A. lanata on oxalate mediated free radical toxicity in ethylene glycol induced calcium oxalate urolithic rats. Calcium oxalate (CaOX) stone was induced by 0.75% ethylene glycol in drinking water for 28 days. From $29^{th}$ day onwards, the CaOX urolithic rats were treated with A. lanata aqueous suspension (2,000 mg/kg body weight/dose/day) orally for another 28 days. At the end of experimental periods the animals were sacrificed, samples were collected and analyzed the lipid peroxidation product, protein oxidation product, enzymatic and non-enzymatic antioxidants in normal and experimental groups. Lipid peroxidation and protein oxidation products were significantly elevated while enzymatic and non-enzymatic antioxidant levels were significantly decreased in ethylene glycol induced CaOX urolithic rats when compared with control rats. The above alterations were reverted to near control in rats treated with aqueous suspension of A. lanata. This study suggests that A. lanata could prevent the free radical formation from calcium oxalate urolithiasis in rats and protecting the renal cells from oxidative injury.
Secondary metabolites (Triterpenes) from Couroupita guianensis
Begum, Rokeya,Rahman, Mohammad S,Chowdhury, A M Sarwaruddin,Hasan, Choudhury M,Rashid, Mohammad A Kyung Hee Oriental Medicine Research Center 2009 Oriental pharmacy and experimental medicine Vol.9 No.2
The n-hexane and carbon tetrachloride soluble fractions of a methanolic extract of the stem bark of the Couroupita guianensis furnished three compounds, identified as $\beta$-amyrin (1), betulin-$3{\beta}$-caffeate (2) and lupeol-$3{\beta}$-caffeate (3). The structures of the isolated compounds were deduced by extensive spectroscopic analysis as well as by comparison with published values. Compounds 1-3 were subjected to antioxidant screening through free radical scavenging activity by DPPH (1,1-diphenyl-2-picrylhydrazyl), where compound 2 showed moderate antioxidant activity with $IC_{50}$ value $108.0{\mu}g/ml$.