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Battle, Katrina N.,Jackson, Joshua M.,Witek, Małgorzata A.,Hupert, Mateusz L.,Hunsucker, Sally A.,Armistead, Paul M.,Soper, Steven A. The Royal Society of Chemistry 2014 The Analyst Vol.139 No.6
<P>We present a novel microfluidic solid-phase extraction (μSPE) device for the affinity enrichment of biotinylated membrane proteins from whole cell lysates. The device offers features that address challenges currently associated with the extraction and purification of membrane proteins from whole cell lysates, including the ability to release the enriched membrane protein fraction from the extraction surface so that they are available for downstream processing. The extraction bed was fabricated in PMMA using hot embossing and was comprised of 3600 micropillars. Activation of the PMMA micropillars by UV/O<SUB>3</SUB> treatment permitted generation of surface-confined carboxylic acid groups and the covalent attachment of NeutrAvidin onto the μSPE device surfaces, which was used to affinity select biotinylated MCF-7 membrane proteins directly from whole cell lysates. The inclusion of a disulfide linker within the biotin moiety permitted release of the isolated membrane proteins <I>via</I> DTT incubation. Very low levels (∼20 fmol) of membrane proteins could be isolated and recovered with ∼89% efficiency with a bed capacity of 1.7 pmol. Western blotting indicated no traces of cytosolic proteins in the membrane protein fraction as compared to significant contamination using a commercial detergent-based method. We highlight future avenues for enhanced extraction efficiency and increased dynamic range of the μSPE device using computational simulations of different micropillar geometries to guide future device designs.</P> <P>Graphic Abstract</P><P>We present a novel microfluidic solid-phase extraction (μSPE) device for the affinity enrichment of biotinylated membrane proteins from whole cell lysates. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3an02400h'> </P>
Disparity in opinions on lip protrusiveness in contemporary African American faces
Yvette Battle,Monica Schneider,Laurence Magder,배응권 대한치과교정학회 2018 대한치과교정학회지 Vol.48 No.1
Objective: In accordance with the changing demographics in the United States, orthodontists working on various ethnic populations should be more conscious when using the standardized profile analyses for the African American patient. The objective of this study was to examine whether the perception of lip protrusiveness in modern African American faces has changed. For this purpose, we investigated the most favorable African American lip profile using the opinions of 10 experienced and 10 newly trained younger orthodontists. Methods: Attractiveness was converted to a number on visualized analog scales. Comparative ranks on 16 African American profiles, with focus on lip protrusiveness and thickness, were made among the groups. Mixed-effects linear regression models were fit and group differences were estimated. Results: Younger orthodontists favored a more protrusive lip profile, and the variance in their perceptions was narrower than those of older orthodontists. Measurements related to upper lip protrusion showed the strongest correlation to attractiveness (r = −0.82). The association with attractiveness decreased linearly as the protrusiveness of the upper lip increased. Steiner’s E-line was the most influential reference for determining the level of attractiveness for the older orthodontists, whereas upper lip protrusion was the most influential factor for the young orthodontists. Conclusions: An adequate level of lip protrusiveness and thickness should be essential for maintaining attractive esthetics in African American patients. Yet, a new set of standards for prominent lips in this population is necessary to reflect the current trend in the concept of a beautiful face in the modern world.[Korean J Orthod 2018;48(1):23-29]
OBSERVATIONS OF THE NEAR-INFRARED SPECTRUM OF THE ZODIACAL LIGHT WITH CIBER
Tsumura, K.,Battle, J.,Bock, J.,Cooray, A.,Hristov, V.,Keating, B.,Lee, D. H.,Levenson, L. R.,Mason, P.,Matsumoto, T.,Matsuura, S.,Nam, U. W.,Renbarger, T.,Sullivan, I.,Suzuki, K.,Wada, T.,Zemcov, M. IOP Publishing 2010 The Astrophysical journal Vol.719 No.1
Yang, Q,Battle, R,Zhang, C,Ma, S M,Seo, J T,Tabibi, B,Temple, D,Sun, S,Jung, S S,Namkung, M American Scientific Publishers 2009 Journal of nanoscience and nanotechnology Vol.9 No.2
<P>The third-order nonlinearity of a PPV-based nanostructured supramolecular organic semiconductor (DBAB), with an electron donor (D) connected to an electron acceptor (A) via nonconjugated and flexible bridge (B) units, was investigated in this work at both near-resonant (532 nm) and nonresonant (1064 nm) wavelength by using degenerate four-wave mixing. The second hyperpolarizabilities of D, A, and DBAB at 532 nm were found to be approximately 2.42 x 10(-43) m2/V2, 7.75 x 10(-44) m2/V2, and 1.80 x 10(-43) m2/V2 in copolarization geometry, and approximately 1.59 x 10(-43) m2/V2, 2.59 x 10(-44) m2/V2, and 1.18 x 10(-43) m2/V2 in orthogonal polarization geometry, respectively. The second hyperpolarizabilities of DBAB at 1064 nm were approximately 1.66 x 10(-46) m2/V2 and approximately 8.77 x 10(-47) m2/V2 for parallel and orthogonal polarization cases.</P>
THE <i>COSMIC INFRARED BACKGROUND EXPERIMENT</i> ( <i>CIBER</i> ): THE LOW RESOLUTION SPECTROMETER
Tsumura, K.,Arai, T.,Battle, J.,Bock, J.,Brown, S.,Cooray, A.,Hristov, V.,Keating, B.,Kim, M. G.,Lee, D. H.,Levenson, L. R.,Lykke, K.,Mason, P.,Matsumoto, T.,Matsuura, S.,Murata, K.,Nam, U. W.,Renbarg IOP Publishing 2013 The Astrophysical journal Supplement series Vol.207 No.2
THE <i>COSMIC INFRARED BACKGROUND EXPERIMENT</i> ( <i>CIBER</i> ): THE NARROW-BAND SPECTROMETER
Korngut, P. M.,Renbarger, T.,Arai, T.,Battle, J.,Bock, J.,Brown, S. W.,Cooray, A.,Hristov, V.,Keating, B.,Kim, M. G.,Lanz, A.,Lee, D. H.,Levenson, L. R.,Lykke, K. R.,Mason, P.,Matsumoto, T.,Matsuura, IOP Publishing 2013 The Astrophysical journal, Supplement series Vol.207 No.2
THE <i>COSMIC INFRARED BACKGROUND EXPERIMENT</i> ( <i>CIBER</i> ): THE WIDE-FIELD IMAGERS
Bock, J.,Sullivan, I.,Arai, T.,Battle, J.,Cooray, A.,Hristov, V.,Keating, B.,Kim, M. G.,Lam, A. C.,Lee, D. H.,Levenson, L. R.,Mason, P.,Matsumoto, T.,Matsuura, S.,Mitchell-Wynne, K.,Nam, U. W.,Renbarg IOP Publishing 2013 The Astrophysical journal, Supplement series Vol.207 No.2
Prevalence and Correlates of Current and Former Smoking among Urban Transit Workers
Cunradi, Carol B.,Moore, Roland S.,Battle, Robynn S. Occupational Safety and Health Research Institute 2017 Safety and health at work Vol.8 No.4
Transit workers constitute a blue-collar occupational group that have elevated smoking rates relative to other sectors of employed adults in the United States. This study analyzed cross-sectional tobacco survey data from 935 workers (60% African American; 37% female) employed at an urban public transit agency in California. Prevalence of current and former smoking was 20.3% and 20.6%, respectively. Younger workers were less likely than older workers to be current or former smokers. Having a complete home smoking ban was associated with decreased likelihood of being a smoker [odds ratio (OR) = 0.04, 95% confidence interval (CI) = 0.01-0.17], as were neutral views about whether it is easy for a smoker to take a smoking break during their shift (OR = 0.50, 95% CI 0.28-0.88). Current smoking among the sample is > 50% higher than the adult statewide prevalence. Potential points of intervention identified in this study include perceived ease of worksite smoking breaks and establishing home smoking bans. Tailored cessation efforts focusing on older transit workers more likely to smoke are needed to reduce tobacco-related disparities in this workforce.
Observation of the Cosmic Near-Infrared Background with the CIBER rocket
MinGyu Kim,T. Matsumoto,Hyung Mok Lee,T. Arai,J. Battle,J. Bock,S. Brown,A. Cooray,V. Hristov,B. Keating,P. Korngut,Dae-Hee Lee,L. R. Levenson,K. Lykke,P. Mason,S. Matsuura,U. W. Nam,T. Renbarger,A. S 한국천문학회 2012 天文學會報 Vol.37 No.1