http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Expression of Mature Thaumatin in Escherichia coli
함영태,Batt, Carl A. 中央大學校 遺傳工學硏究所 1991 遺傳工學硏究論集 Vol.4 No.1
Thaumatin, a sweet-tasting plant protein, is initially produced as a precursor, preprothaumatin. The presignal sequence at aminoterminal end containing 22 mostly hydrophobic amino acid residues and the prosignal sequence at carboxy-terminal end carrying 6 mainly acidic amino acid residues of preprothaumatin II were removed by using a synthetic oligonucleotide and site-specific mutagenesis to produce mature thaumatin in E. coli. The presignal sequence was replaced by a 34mer oligonucleotide carrying a Shine-Dalgarno sequence and a translational initiation codon. The first amino acid of prosignal sequence, leu-230, was changed to an ochre stop codon TAA. The met-thaumatin gene in the plasmid pUTH-3 carrying two copies of the lac UV5 promoters was expressed in E. coli. A trace amount of an immunoreactive 22 kDa protein of a molecular weight similar to mature thaumatin was observed in E. coli SVS-3 [pUTH-3].
Multiplexed Protein Patterns on a Photosensitive Hydrophilic Polymer Matrix
Bhatnagar, Parijat,Malliaras, George G.,Kim, Il,Batt, Carl A. WILEY-VCH Verlag 2010 Advanced Materials Vol.22 No.11
<B>Graphic Abstract</B> <P>A photosensitive hydrogel film containing a photocleavable protecting group is attached to silicon oxide. Projection photolithography is used to generate carboxylic groups in selective patterns on this film, to which proteins are coupled through their primary amine groups. The carboxylic groups on the proteins are chemically protected and the process is repeated to sequentially pattern additional proteins on the hydrogel surface. <img src='wiley_img_2010/09359648-2010-22-11-ADMA200903255-content.gif' alt='wiley_img_2010/09359648-2010-22-11-ADMA200903255-content'> </P>