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- 저자 : Barile, (검색결과 12 건)
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Lee, H.,MeloSilva, V.L.d.,Liu, Y.,Barile, D. American Dairy Science Association 2015 Journal of dairy science Vol.98 No.11
A method was developed for the characterization and quantification of the disaccharide lactose and 3 major bovine milk oligosaccharides (BMO) in dairy streams. Based on high-performance anion-exchange chromatography-pulsed amperometric detection (HPAE-PAD), this method is advantageous because it requires minimal sample preparation and achieves good chromatographic separation of oligosaccharide isomers within 30min. The linear dynamic range and limit of detection were 0.1 to 10mg/L and 0.03 to 0.22mg/L, respectively. Mean recoveries of the BMO were excellent and ranged from 98.4 to 100.4%. Without complicated sample preparation procedures, this HPAE-PAD method measured BMO [3'-sialyllactose (3'SL), 6'-sialyllactose (6'SL), and 6'-sialyllactosamine (6'SLN)] and lactose using a single instrument, therefore increasing the accuracy of the measurement and applicability for the dairy industry. In colostrum whey permeate, 3'SL, 6'SL, and 6'SLN were 94, 29, and 46mg/L, respectively. This work is the first to demonstrate that some commercial products, currently marketed for supporting a healthy immune system, contain significant amounts of bioactive BMO and therefore, carry additional bioactivities.
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de Souza Bastos, Leonardo,Guerrero, Carolina Andrea Sanchez,Barile, Alan,da Silva, Jose Guilherme Santos Techno-Press 2019 Coupled systems mechanics Vol.8 No.2
This research work presents a study that aims to assess the dynamic structural behaviour and also investigate the human comfort levels of a reinforced concrete building, when subjected to nondeterministic wind dynamic loadings, considering the effect of masonry infills on the global stiffness of the structural model. In general, the masonry fills most of the empty areas within the structural frames of the buildings. Although these masonry infills present structural stiffness, the common practice of engineers is to adopt them as static loads, disregarding the effect of the masonry infills on the global stiffness of the structural system. This way, in this study a numerical model based on sixteen-storey reinforced concrete building with 48 m high and dimensions of $14.20m{\times}15m$ was analysed. This way, static, modal and dynamic analyses were carried out in order to simulate the structural model based on two different strategies: no masonry infills and masonry infills simulated by shell finite elements. In this investigation, the wind action is considered as a nondeterministic process with unstable properties and also random characteristics. The fluctuating parcel of the wind is decomposed into a finite number of harmonic functions proportional to the structure resonant frequency with phase angles randomly determined. The nondeterministic dynamic analysis clearly demonstrates the relevance of a more realistic numerical modelling of the masonry infills, due to the modifications on the global structural stiffness of the building. The maximum displacements and peak accelerations values were reduced when the effect of the masonry infills (structural stiffness) were considered in the dynamic analysis. Finally, it can be concluded that the human comfort evaluation of the sixteen-storey reinforced concrete building can be altered in a favourable way to design.
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Lee, Hyeyoung,Nobrega de Moura Bell, Juliana Maria Leite,Barile, Daniela American Chemical Society 2019 Journal of agricultural and food chemistry Vol.67 No.12
<P>Bovine milk oligosaccharides (BMOs) that resemble human milk oligosaccharides are found in whey permeate, indicating that dairy streams can be used as a potential source of bioactive oligosaccharides. Recovery of oligosaccharides from whey permeate is hindered by their low abundance and high concentration of lactose. In the present work, lactose in bovine colostrum whey permeate was hydrolyzed by <I>Aspergillus oryzae</I> β-galactosidase to facilitate subsequent monosaccharide removal by membrane separation. Chromatographic separation coupled with high-resolution mass spectrometry revealed β-galactosidase degradation of several β-linkage-containing BMOs and production of novel oligosaccharides that ranged in size from 5 to 11 monosaccharide units containing several galactose repeating units and <I>N</I>-acetylhexosamine at their reducing ends. Optimization of BMO hydrolysis and separation methodology could generate high amounts of hetero-oligosaccharides for improved recovery of potentially biotherapeutic oligosaccharides.</P> [FIG OMISSION]</BR>
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Tracing flavonoid degradation in grapes by MS filtering with stable isotopes
Chassy, Alexander W.,Bueschl, Christoph,Lee, Hyeyoung,Lerno, Larry,Oberholster, Anita,Barile, Daniela,Schuhmacher, Rainer,Waterhouse, Andrew L. Elsevier 2015 Food chemistry Vol.166 No.-
<P><B>Abstract</B></P> <P>Anthocyanin degradation has been proposed as one of the primary causes for reduced colour and quality in red wine grapes grown in a warm climate. To study anthocyanin degradation we infused berries with <SMALL>L</SMALL>-phenyl-<SUP>13</SUP>C<SUB>6</SUB>-alanine and then tracked the fate of the anthocyanins comparing normal (25°C) and warm (45°C) temperature conditions. An untargeted metabolomics approach was aided by filtering the MS data using software algorithms to extract all M and M+6 isotopic peak pairs, allowing the analysis to focus solely on the metabolites of phenylalanine. A paired-comparison <I>t</I>-test was performed over the 8 biological replicates revealing 13 metabolites that were statistically different between 25°C and 45°C treatments. Most of these features had lower abundances in 45°C samples, confirming that 45°C treatment caused anthocyanin degradation. In addition, resveratrol was significantly reduced following heat treatment. However, 5 metabolites increased following the 45°C treatment. These unidentified metabolites are therefore suspects for anthocyanin degradation products.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We combined <SUP>13</SUP>C isotopic labelling and LC-HRMS based metabolomics. </LI> <LI> Phenylalanine-derived compounds in grapes were filtered using untargeted profiling. </LI> <LI> Thermal degradation products of known and unknown berry metabolites were detected. </LI> <LI> Warm temperature (45°C) treatment decreased anthocyanins as expected. </LI> <LI> Warm temperature produced 5 metabolites that may be anthocyanin degradation products. </LI> </UL> </P>
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Lee, Hyeyoung,Cuthbertson, Daniel J.,Otter, Don E.,Barile, Daniela American Chemical Society 2016 Journal of agricultural and food chemistry Vol.64 No.32
<P>A bovine milk oligosaccharide (BMO) library, prepared from cow colostrum, with 34 structures was generated and used to rapidly screen oligosaccharides in domestic animal milks and a whey permeate powder. The novel library was entered into a custom Personal Compound Database and Library (PCDL) and included accurate mass, retention time, and tandem mass spectra. Oligosaccharides in minute-sized samples were separated using nanoliquid chromatography (nanoLC) coupled to a high resolution and sensitive quadrupole-Time of Flight (Q-ToF) MS system. Using the PCDL, 18 oligosaccharides were found in a BMO-enriched product obtained from whey permeate processing. The usefulness of the analytical system and BMO library was further validated using milks from domestic sheep and buffaloes. Through BMO PCDL searching, 15 and 13 oligosaccharides in the BMO library were assigned in sheep and buffalo milks, respectively, thus demonstrating significant overlap between oligosaccharides in bovine (cow and buffalo) and ovine (sheep) milks. This method was shown to be an efficient, reliable, and rapid tool to identify oligosaccharide structures using automated spectral matching.</P>
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de Moura Bell, J.M.L.N.,Aquino, L.F.M.C.,Liu, Y.,Cohen, J.L.,Lee, H.,de Melo Silva, V.L.,Rodrigues, M.I.,Barile, D. American Dairy Science Association 2016 Journal of dairy science Vol.99 No.8
<P>Enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. Achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. Response surface methodology was used to investigate the effects of pH (3.25-8.25), temperature (35-55 degrees C), reaction time (6 to 58 min), and amount of enzyme (0.05-0.25%) on the efficiency of lactose hydrolysis by beta-galactosidase and on the preservation of biologically important sialyloligosaccharides (3'-siallylactose, 6-siallylactose, and 6'-sialyl-N-acetyllactosamine) naturally present in bovine colostrum whey permeate. A central composite rotatable design was used. In general, beta-galactosidase activity was favored at pH values ranging from 3.25 to 5.75, with other operational parameters having a less pronounced effect. A pH of 4.5 allowed for the use of a shorter reaction time (19 min), lower temperature (40 degrees C), and reduced amount of enzyme (0.1%), but complete hydrolysis at a higher pH (5.75) required greater values for these operational parameters. The total amount of sialyloligosaccharides was not significantly altered by the reaction parameters evaluated, suggesting specificity of beta-galactosidase from Aspergillus oryzae toward lactose as well as the stability of the oligosaccharides at pH, temperature, and reaction time evaluated.</P>
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High photocatalytic capacity of porous ceramic-based powder doped with MgO
Bouras Dikra,Fellah Mamoun,Mecif Abla,Barillé Régis,Obrosov Aleksei,Rasheed Mohammed 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.1
To purify water at low cost for our daily life, the effect of ceramic-based (mullite–cristobalite) and (mullite–zircon) powders doped with different amounts of magnesium oxide (MgO) (10 and 20 wt%) was studied. These compounds are made of a local raw material DD3 with addition of zirconia (ZrO2) to create an open porosity. The powders were prepared by the traditional mixing method with the help of an automated crushing. The effect of MgO doping on structural, morphological and photocatalytic properties of the material was studied by various analytical techniques such as X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, infrared, and UV–visible spectrometry. The results of XRD proved that there was a deformation in the crystal structure of the two types of ceramics after addition, which resulted in a shift of the spectra to the right, while SEM proved the presence of pores with a larger size as the proportion of MgO increases. The chemical composition of the basic components of the ceramic compounds as well as the additive was confirmed with EDS and IR spectra. The final results show that ceramics with added zirconia (DD3Z) and doped with 10% of MgO have a better photocatalytic efficiency than ceramics without zirconia. This important effect could be related to the higher rate of porosity, which provides a more active surface. The 10% MgO content showed a high photoactivity of 77.33% in only 15 min. The maximum hydrolysis rate obtained with Orange II was 92.95% after a period of 45 min with DD3Z/MgO powders.
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