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Baptista, F.,Castro-Prado, M.A.A. The Microbiological Society of Korea 2001 The journal of microbiology Vol.39 No.2
DNA damage response has a central role in the maintenance of genomic integrity while mutations in related genes may result in a range of disorders including neoplasic formations. The uvsZl characterized in this report is a navel uvs mutation in Aspergillus nidulans, resulting in a nucleotide excision repair (NER) phenotype: UV-sensitivity before DNA synthesis (quiescent cells), high UV-induced mutation frequency and probable absence of involvement with mitotic and meiotic recombinations. The mutation is recessive and nan-allelic to the previously characterized uvsA101 mutation, also located on the paba-y interval on chromosome I. uvsZl skewed wild-type sensitivity to MMS, which suggests non-involvement of this mutation with BER. Epitasis tests showed that the uvsZ gene product is probably involved in the same repair pathways as UVSB or UVSH proteins. Although mutations in these proteins result in an NER phenotype, UVSB is related with cell cycle control and UVSH is associated with the post-replicational repair pathway. The epistatic interaction among uvsZl and uvsB413 and uvsH77 mutations indicates that different repair systems may be related with the common steps of DNA damage response in Aspergillus nidulans.
DIGITAL ECOSYSTEMS AND ITS INFLUENCE ON BUSINESS RELATIONSHIPS
Cristina Baptista,Jos? Novais,Jo?o Mota,Danilo Nunes 글로벌지식마케팅경영학회 2023 Global Marketing Conference Vol.2023 No.07
Digitalization is a prevailing force in the marketplace and unquestionably a key topic on the strategic agenda of many firms (e.g., Balsmeier & Woerter, 2019). Alongside, research into managing digitalization has also strongly increased in recent years (Svahn, Matthiasen, & Lindgren, 2017; Ritter & Pedersen, 2020). Hofacker, Pillai, Golgeci & Gligor (2020, p. 1162) claim “Artificial intelligence, blockchain, data security/integrity, Internet of Things, and big data analytics are just some possible digital trends that can shape how B2B relationships are understood and managed”. Despite this increasing interest, the number of works within the scope of business-to-business (B2B) exchanges is still limited (Obal & Lancioni, 2013; Pagani & Pardo, 2017; Gomes et al., 2022; Matt, Pedrini, Bonfanti & Orzes, 2023) and there any many unexplored or underexplored phenomena.
[Poster Presentation] IS THE EXTRACTIVES PLAY ANY POLE IN COLORING PULPS FROM Pinus pinaster?
C.Baptista,M.N.Belgacem,A.P.Duarte 한국펄프·종이공학회 1999 한국펄프종이학회 기타 간행물 Vol.- No.-
Pulps from Pinus pinaster were obtained using classical and modified kraft processes of pulping with the aim objective of establishing whether the extractives contribute in inducing the dark colour of the unbleached pulps from this specie. The methodology adopted here was based on carrying out two set of experiments. The first one consisted on cooking extractive-free wood chips whereas the second one was devoted to the pulping of wood in the presence of a surfactant. The colour of the pulps thus obtained were compared to that of the pulps prepared from initial wood cooked without any modification. In the first set of experiments, the wood chips were submitted to extraction wit different solvents and acetone and toluene / ethanol appeared to be the most efficient, since the pulps obtained from wood previously extracted by this solvent showed that the brightness of pulps increased by more than 12% and the kappa number decreased by about 4 points. In second set of experiment, the wood chips were cooked in the presence of non-ionic surfactants from poly(ethylene), PEG, and poly(propylene) glycol PPG, homopolymers and copolymers with molecular weights ranging from 400 to<br/> 10000. The most promising tensio-active compounds<br/> were PEG and a block copolymer PEG-PPG-PEG with molecular weight of 1000 and 1100, respectively. The optimal concentration of the surfactants was about 4%. The kappa number of these pulps lowered significantly, i.e. from 50 for the reference to 39 for the pulps obtained by kraft cooking in the presence of 4% of PEG 1000.
( Brian M Baptista ),( Ninfa S Asia ) 한국행정학회 2016 한국행정학회 학술발표논문집 Vol.2016 No.-
This descriptive-correlational study determined the extent of compliance of Local Government Units (LGUs) of Ilocos Norte on the Citizen’s Charter (CC) and their clients’ satisfaction on the quality of basic service delivery. Thirteen LGUs were selected using proportional stratified sampling based on their income classification in 2011. Seven offices to represent each LGU were, in turn, chosen via purposive sampling. For each LGU, 15 service providers and 60 service seekers were randomly and purposively selected, respectively, to serve as respondents. On the whole, there were 961 respondents of the study: 181 service providers and 780 service seekers Statistical tools such as means, paired-samples t-test, and Pearson r were used in analyzing the data with the aid of the Statistical Package for Social Sciences (SPSS). Eight of the LGUs in study were categorized as third and fourth class municipalities and two, fifth class municipalities. On the average, 57 personnel from these LGUs were involved in the implementation of the CC, while an average of eight attended trainings/workshops on CC. Clients seeking services in these LGUs ranged from 14-160 per day. Majority of them implemented their respective CCs in 2009. Both service providers and service seekers perceived the LGUs to have complied to a great extent with the CC provisions. Their ratings, however, had significant differences. On the other hand, the clients were highly satisfied on the quality of basic services delivered by the LGUs. All six indicators on clients’ satisfaction on the service quality dimensions of CC were found out to have highly significant correlation with the LGUs’ extent of compliance on procedural clarity and grievance redress. Requests, one indicator of clients’ satisfaction on the quality of basic services, was found to have highly significant correlation with the LGUs’ extent of compliance on procedural clarity, and a significant correlation with the indicator issuances and payments. On the other hand, the LGUs’ extent of compliance with grievance redress had a highly significant relationship with all the three indicators of LGUs client’s satisfaction on the quality of basic services: issuances, requests, and payments.
General FRET-based coding for application in multiplexing methods
Giestas, Leticia,Petrov, Vesselin,Baptista, Pedro V.,Lima, Joao Carlos Korean Society of Photoscience 2009 Photochemical & photobiological sciences Vol.8 No.8
FRET can be used as a strategy to assign different simultaneous events in the same sample but "cross-talk" problems are a limitation. Here we present a contribution for the better understanding of the "cross-talk" in FRET experiments that include several pairs in the same sample. Using oligonucleotide probes labeled with fluorescent dyes which can be selectively excited at a specific wavelength, and using target oligonucleotides tagged with a fluorescent dye with specific characteristics that allow only it to emit light upon selective excitation of a specific probe by energy transfer (FRET), we aim to identify the exact probe-target hybridized pair. When using three donors to probe the presence of complementary targets, only 20% of possible donor/acceptor combinations give straightforward signals readily identifiable with the sample composition, while in the remaining cases severe cross-excitation prevents the direct identification of the sample composition. To correctly resolve the samples identity, we developed a theoretical model that enables the unequivocal attribution of a sample composition to a given set of fluorescence signals, in the presence of three donors.
Alexandre Negrao Pantaleao,Anna Luísa Mennitti,Felipe Baptista Brunheroto,Vitória Stavis,Laura Teresa Ricoboni,Victor Augusto Fonseca de Castro,Ollivia Frederigue Ferreira,Eura Martins Lage,Deborah Ri 대한의료정보학회 2024 Healthcare Informatics Research Vol.30 No.1
Objectives: Digital health (DH) is a revolution driven by digital technologies to improve health. Despite the importance ofDH, curricular updates in healthcare university programs are scarce, and DH remains undervalued. Therefore, this reportdescribes the first Junior Scientific Committee (JSC) focusing on DH at a nationwide congress, with the aim of affirming itsimportance for promoting DH in universities. Methods: The scientific committee of the Brazilian Congress of Health Informatics(CBIS) extended invitations to students engaged in health-related fields, who were tasked with organizing a warm-upevent and a 4-hour session at CBIS. Additionally, they were encouraged to take an active role in a workshop alongside distinguishedexperts to map out the current state of DH in Brazil. Results: The warm-up event focused on the topic “Artificialintelligence in healthcare: is a new concept of health about to arise?” and featured remote discussions by three professionalsfrom diverse disciplines. At CBIS, the JSC’s inaugural presentation concentrated on delineating the present state of DH educationin Brazil, while the second presentation offered strategies to advance DH, incorporating viewpoints from within andbeyond the academic sphere. During the workshop, participants deliberated on the most crucial competencies for future professionalsin the DH domain. Conclusions: Forming a JSC proved to be a valuable tool to foster DH, particularly due to thevaluable interactions it facilitated between esteemed professionals and students. It also supports the cultivation of leadershipskills in DH, a field that has not yet received the recognition it deserves.