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Baolei Du,Hongjiu Su,Shudong Wang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
Carbon nanofiber (CNF) coated monoliths with a homogeneous and consistent layer were prepared bycatalytic decomposition of ethane on Ni particles, and the CNF coated monolith supports were oxidizedusing different agents to get different oxidation degrees. The as-prepared catalysts were characterized bySEM, TG, Raman spectra, FT-IR, TPD, TPR, CO pulsar and TEM, while the behavior of Pd supported on CNFcoated monoliths were tested by liquid phase of nitrobenzene hydrogenation in a continuous flowreactor. For comparison Pd/Al2O3/cordierite was also prepared. It was found that the reductiontemperature controlled Pd dispersion, which in turn was responsible for the catalytic activity. A goodlinear relationship between nitrobenzene conversion and Pd dispersion was observed. Furthermore, Pd/CNF/cordierite showed higher nitrobenzene conversion compared with Pd/Al2O3/cordierite, indicatingCNF was a superior support. The aniline selectivity was related to the amount of surface oxygen groups(SOCs) formed during the oxidation pre-treatment. SOCs, especially carboxylic acid groups, increased asthe degree of oxidation deepened, and more amounts of SOCs on CNF surface were responsible for thelower aniline selectivity.
An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress
Baolei Jia,이상민,Bang P. Pham,조윤승,양재경,변희섭,김종철,정강원 한국분자세포생물학회 2010 Molecules and cells Vol.29 No.4
NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to H2O2 or four-electron reduction of oxygen to H2O. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to β-amyloid and Lewy bodies in neurodegenerative diseases, including an increase of β-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce H2O2. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treatment with H2O2. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles,we suggest that NOXtp may be an ancestor of cell death proteins.
Liu, Baolei,Xu, Jinli,Xu, Wei,Xia, Wei The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.10
Accurate estimation of state of charge is essential to ensure reliable and efficient management of lithium-ion batteries in electric vehicles. The working procedure of lithium-ion batteries is very sophisticated. Thus, developing novel methods for SOC estimation in nonlinear non-Gaussian battery system is inevitable. In this article, a novel adaptive cubature H-infinity filter (ACHF) is proposed. It combines the favorable characteristics of H-infinity (HF) and cubature Kalman filter (CKF). In the iterative process of CKF, the singular value decomposition is used to guarantee non-negative definiteness of the error covariance matrix. The statistical properties of process and measurement noise are timely modified by the Sage-Husa estimator. The performance of the developed method is evaluated by the urban dynamometer driving schedule test. Through the comparison with traditional CKF, the experimental results show that the proposed ACHF method can achieve precise SOC. Moreover, the robustness verification results illustrate that the proposed algorithm is robust to the SOC initial errors.
An Archaeal NADH Oxidase Causes Damage to Both Proteins and Nucleic Acids under Oxidative Stress
Jia, Baolei,Lee, Sang-Min,Pham, Bang P.,Cho, Yoon-Seung,Yang, Jae-Kyung,Byeon, Hee-Seop,Kim, Jong-Cheol,Cheong, Gang-Won Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.4
NADH oxidases (NOXs) catalyze the two-electron reduction of oxygen to $H_2O_2$ or four-electron reduction of oxygen to $H_2O$. In this report, we show that an NADH oxidase from Thermococcus profundus (NOXtp) displays two forms: a native dimeric protein under physiological conditions and an oxidized hexameric form under oxidative stress. Native NOXtp displays high NADH oxidase activity, and oxidized NOXtp can accelerate the aggregation of partially unfolded proteins. The aggregates formed by NOXtp have characteristics similar to ${\beta}$-amyloid and Lewy bodies in neuro-degenerative diseases, including an increase of ${\beta}$-sheet content. Oxidized NOXtp can also bind nucleic acids and cause their degradation by oxidizing NADH to produce $H_2O_2$. Furthermore, Escherichia coli cells expressing NOXtp are less viable than cells not expressing NOXtp after treatment with $H_2O_2$. As NOXtp shares similar features with eukaryotic cell death isozymes and life may have originated from hyperthermophiles, we suggest that NOXtp may be an ancestor of cell death proteins.
Oligomeric Structure of the ATP-dependent Protease La (Lon) of Escherichia coli
박성철,Baolei Jia,양재경,Duyet Le Van,Yong Gi Shao,한상우,전영주,정진하,정강원 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1
Lon, also known as protease La, belongs to a class of ATP-dependent serine protease. It plays an essential role in degradation of abnormal proteins and of certain short-lived regulatory proteins, and is thought to possess a Ser-Lys catalytic dyad. To examine the structural organization of Lon, we performed an electron microscope analysis. The averaged images of Lon with end-on orientation revealed a six-membered, ringshaped structure with a central cavity. The side-on view showed a two-layered structure with an equal distribution of mass across the equatorial plane of the complex. Since a Lon subunit possesses two large regions containing nucleotide binding and proteolytic domains, each layer of the Lon hexamer appears to consist of the side projections of one of the major domains arranged in a ring. Lon showed a strong tendency to form hexamers in the presence of Mg2+, but dissociated into monomers and/or dimers in its absence. Moreover, Mg2+-dependent hexamer formation was independent of ATP. These results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires Mg2+, but not ATP.
Oligomeric structure of the ATP-dependent protease La (Lon) of Escherichia coli.
Park, Seong-Cheol,Jia, Baolei,Yang, Jae-Kyung,Van, Duyet Le,Shao, Yong Gi,Han, Sang Woo,Jeon, Young-Joo,Chung, Chin Ha,Cheong, Gang-Won Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.1
<P>Lon, also known as protease La, belongs to a class of ATP-dependent serine protease. It plays an essential role in degradation of abnormal proteins and of certain short-lived regulatory proteins, and is thought to possess a Ser-Lys catalytic dyad. To examine the structural organization of Lon, we performed an electron microscope analysis. The averaged images of Lon with end-on orientation revealed a six-membered, ring-shaped structure with a central cavity. The side-on view showed a two-layered structure with an equal distribution of mass across the equatorial plane of the complex. Since a Lon subunit possesses two large regions containing nucleotide binding and proteolytic domains, each layer of the Lon hexamer appears to consist of the side projections of one of the major domains arranged in a ring. Lon showed a strong tendency to form hexamers in the presence of Mg(2+), but dissociated into monomers and/or dimers in its absence. Moreover, Mg(2+)-dependent hexamer formation was independent of ATP. These results indicate that Lon has a hexameric ring-shaped structure with a central cavity, and that the establishment of this configuration requires Mg(2+), but not ATP.</P>
Min Su,Hongjiu Su,Baolei Du,Xiaotong Li,Gaoyuan Ren,Shudong Wang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.5
Silica nanoparticles with different sizes (ranging from 10 nm to 104 nm) and size distributions were synthesized by semi-batch and semi-batch/batch methods of the Stöber process. Then the amorphous silica with different surface areas (ranging from 30m2/g to 400m2/g) and pores (ranging from 3 nm to 33 nm) were obtained by various aging treatments and drying methods of the synthesized colloidal silica sol. The aging treatment resulted in the monodispersed pore distribution and decreased BET surface area of silica. The high-humidity drying method led to the mesoporous silica with uniform pores and decreased small pores. As the silica was obtained by the arrangement of silica nanoparticles, the pore diameter and pore distribution of mesoporous silica were directly related to the size and distribution of nanoparticles. Furthermore, this study offered a new thought for the synthesis of other mesoporous materials with uniform pore distributions.