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Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2
Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4
Lentinula edodes is an important cultivated mushroom in China. The development of Lentinula edodes production promotes more studies on it. In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.
THE RELIABLE DESIGN OF THE CIM NETWORK FOR A LARGE-SCALE MACHINE TOOL FACTORY
Yan, Bao Ping,Zhou, Jin Ming,Bao Shcng Hu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1
This paper discusses the reliability problems during designing the JFMT (Jinan First Machine Tool works) CIM network. At first the sources which affect the reliability of JFMT CIM network are analysed. And then some solutions for enhancing the reliability of JFMT CIM network are given. These methods are also suitable to other CIM networks design and of certain practicality.
Shi, Bao-ming,Shan, An-shan,Tong, Jian-ming Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.12
An experiment was conducted to determine the effects of dietary oligosaccharides on performance and intestinal microbial populations of piglets. Ten litters of piglets were assigned to five groups randomly, with two litters per group. The control group was fed with corn-soybean basal diet. Oligosaccharides was added to the basal diet at the level of 0.05%, 0.1%, 0.2% and 0.35% respectively to form four experimental diets. The experiment was conducted with two periods. The first period (suckling period) was from 7 to 28 days of age and the second period (weanling period) was from 28 to 56 days of age. Fresh fecal samples were collected at 21 days of age and assayed for Escherichia coli concentration, pH and moisture content. Three pigs per group were slaughtered at 42 days of age and cecum, colon, and rectum content samples were collected and assayed immediately for Escherichia coli and Bifidobacterium concentration, pH and moisture content. The results showed that dietary oligosacchrides decreased fecal Escherichia coli population and pH significantly (p 0.05), but did not affect performance and fecal moisture content during suckling period. 0.1% oligosaccharides for weanling pigs increased growth and improved feed conversion ratio together with a reduction of diarrhea (p 0.05), but 0.35% oligosaccharides did not affect growth performance. 0.1% and 0.2% oligosaccharides for weanling pigs had a suppression to Escherichia coli colonization in rectum and an enrichment to Bifidobacterium in colon (p 0.05). Oligosaccharides decreased significantly (p<0.05) rectum moisture content, but did not affect cecum, colon and rectum pH.
Roles of NMDA NR2B Subtype Receptor in Prefrontal Long-Term Potentiation and Contextual Fear Memory
Zhao, Ming-Gao,Toyoda, Hiroki,Lee, Yong-Seok,Wu, Long-Jun,Ko, Shanelle W.,Zhang, Xue-Han,Jia, Yongheng,Shum, Fanny,Xu, Hui,Li, Bao-Ming,Kaang, Bong-Kiun,Zhuo, Min Elsevier 2005 Neuron Vol.47 No.6
<P><B>Summary</B></P><P>Cortical plasticity is thought to be important for the establishment, consolidation, and retrieval of permanent memory. Hippocampal long-term potentiation (LTP), a cellular mechanism of learning and memory, requires the activation of glutamate N-methyl-D-aspartate (NMDA) receptors. In particular, it has been suggested that NR2A-containing NMDA receptors are involved in LTP induction, whereas NR2B-containing receptors are involved in LTD induction in the hippocampus. However, LTP in the prefrontal cortex is less well characterized than in the hippocampus. Here we report that the activation of the NR2B and NR2A subunits of the NMDA receptor is critical for the induction of cingulate LTP, regardless of the induction protocol. Furthermore, pharmacological or genetic blockade of the NR2B subunit in the cingulate cortex impaired the formation of early contextual fear memory. Our results demonstrate that the NR2B subunit of the NMDA receptor in the prefrontal cortex is critically involved in both LTP and contextual memory.</P>
Bao-Guang Zhang,Xiao-Ming Zhang,Hua-Bing Li,Hai-Tao Liu 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.3
As a strong and low-priced austenite stabilizer, nitrogen (0.085 wt%) was deliberately added into a hot-rolled Fe-0.2C-5Mn TRIP steel for optimizing the strength–ductility balance. Nitrogen addition can not only increase austenite fraction (> 50%), but also favor the improvement of austenite stability due to its partition behavior from ferrite to austenite during intercritical annealing. Besides, the low annealing temperature (635 °C) and nitrogen addition result in the simultaneous precipitation of cementite (size, 21–396 nm) and carbonitride (size, 7–193 nm) in ferrite and at grain boundaries, which can effectively increase the yield strength through precipitation strengthening (33–63 and 41–61 MPa, respectively). Furthermore, the sufficient interstitial solute originated from N addition as well as the discontinuous TRIP effect benefits the strong serrated flow behavior and multi-peak strain hardening behavior. As a result, the strong TRIP effect together with precipitation strengthening is believed to be responsible for the excellent strength–ductility balance of present steel annealed for 3 h, demonstrating a high tensile strength of 1043 MPa, outstanding total elongation of 43.3% and superior product of strength and elongation (PSE) of 44.8 GPa%.