Effects of Rhodiola rosea and Panax ginseng on the Metabolic Parameters of Rats Submitted to Swimming

Victor Myung Joon Bang,Ana Luisa de Carvalho Arana˜o,Bruna Zampieri Nogueira,Adriano Cressoni Arau´jo,Patricia Cincotto dos Santos Bueno,Sandra Maria Barbalho,Maricelma da Silva Soares de Souza,Elen L 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.10

Adaptogen-based plant formulations play an important role in traditional medicine and have been used in medical practice to increase the resistance of individuals. Rhodiola rosea (RR) and Panax ginseng (PG) exhibit adaptogenic properties and are related to the recovery of homeostasis and strengthen systems impaired by stress. This study aimed to evaluate the effects of RR and PG on metabolic profile and muscle damage parameters in Wistar rats submitted to swimming. Animals were divided according to the following: G1: control group; G2: group that was submitted to swimming; G3: group treated with PG; G4: group treated with PG and submitted to swimming; G5: treated with RR; and G6: treated with RR and submitted to swimming. At the end of the experimental protocol, groups G2, G4, and G6 practiced swimming for a period five times longer than during the previous 30 days. Anthropometric and biochemical parameters were investigated, and no significant results were found in the groups. Nevertheless, animals treated with PG and RR reduced the levels of creatine phosphokinase (CPK) and lactic dehydrogenase (LDH). Our findings demonstrate that both PG and RR produced a significant reduction in the levels of CPK and LDH after physical stress, suggesting that they can be used to improve physical performance. For these reasons, we may say that these plants may be used to minimize the stress promoted by the practice of physical exercises.

FK-3000 isolated from <i>Stephania delavayi</i> Diels. inhibits MDA-MB-231 cell proliferation by decreasing NF-κB phosphorylation and COX-2 expression

DE XU, HONG,CHO, SOON-CHANG,BANG, MI-AE,BAE, CHUN-SIK,CHOI, YEONSHIK,LI, YONG-CHUN,LIM, SEUNG-KIL,SHIM, JAEGAL,PARK, DAE-HUN D.A. Spandidos 2015 International journal of oncology Vol.46 No.6

<P>The World Health Organization (WHO) has reported that cancer is one of the most prevalent diseases and a leading cause of death worldwide. Many anticancer drug development studies have been pursued over the last few decades and several viable drugs have been discovered, such as paclitaxel, topotecan and irinotecan. Previously, our research group uncovered the cytocidal and cytostatic effects of the plant <I>Stephania delavayi</I> Diels. In this study, we determined the active chemical to be 6,7-di-O-acetylsinococuline (FK-3000). The FK-3000 half maximal inhibitory concentration (IC<SUB>50</SUB>) in MDA-MB-231 breast carcinoma cells at 48 h was 0.52 μg/ml and it induced apoptosis in a dose- and time-dependent manner. FK-3000 suppressed NF-κB nuclear translocation, decreased NF-κB phosphorylation, and decreased COX-2 protein expression. MDA-MB-231 xenografted mice were treated with FK-3000, Taxol, or their combination for 21 days. The tumor size was smallest in the co-treatment group, indicating that FK-3000 may have a synergistic effect with Taxol. FK-3000 treatment showed no adverse effects on blood cell counts, serum protein levels, or pathology. These studies demonstrate that FK-3000, isolated from <I>S. delavayi</I> Diels., is a promising, pathway-specific anticancer agent that exhibits low toxicity.</P>

Direct Isolation of the gerB-narA Stretch from the Bacillus subtilis Chromosome by I-SceI Site Insertion

Song, Bang Ho,Fuente, Veronica De La,Glaser, Philippe,Danchin, Antoine 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1

A spectionmycin cassette harboring the I-SceI meganuclease recognition site was inserted into the open reading frame of the Bacillus subtilis narA gene. This fusion vector was integrated into the narA locus of the chromosome and selected by marker rescue with spectinomycin resistance. Subsequent integration of the I-SceI site near the gerB region was performed in B. subtilis (pSO19) which already had the I-SceI ate in the narA gene. The transformant, B. subtilis (pSO19/20), containing I-SceI sites both in the locus narA and in the vicinity of gerB was isolated by selection with spectinomycin and chloramphenicol resistance. Direct isolation of the 70 kbp of narA-gerB stretch from the nested chromosome embedded in an agar plug was obtained by pulsed field gel electrophoresis after cutting both I-SceI sites. By this method, the precise location of these genes and the distance between gerB and narA were determined This unique method is very useful and applicable as a biochemical tool for isolating any intended fragments, for blocking specific loci Eke ate directed mutagenesis or large deletion, and for precise mapping of unknown genes by determination of their chromosomal distance and orientation.

Direct isolation of the gerB-narA Stretch from the Bacillus subtilis chromosome by I-SceI Site Insertion

Song,Bang-Ho,Fuente, Veronica De La,Glaser, Philippe,Danchin, Antonie Korean Society of Molecular Biology 1995 Molecules and cells Vol.5 No.1

Direct Isolation of the gerB-narA Stretch from the Bacillus subtilis Chromosome by I-SceI Site Insertion

Song, Bang Ho,Fuente, Veronica De La,Glaser, Philippe,Danchin, Antoine 경북대학교 유전공학연구소 1996 遺傳工學硏究所報 Vol.11 No.1

A spectionmycin cassette harboring the I-SceI meganuclease recognition site was inserted into the open reading frame of the Bacillus subtilis narA gene. This fusion vector was integrated into the narA locus of the chromosome and selected by marker rescue with spectinomycin resistance. Subsequent integration of the I-SceI site near the gerB region was performed in B. subtilis (pSO19) which already had the I-SceI site in the narA gene. The transformant, B. subtilis (pSO19/20), containing I-SceI sites both in the locus narA and in the vicinity of gerB was isolated by selection with spectinomycin and chloramphenicol resistance. Direct isolation of the 70 kbp of narA-gerB stretch from the nested chromosome embedded in an agar plug was obtained by pulsed field gel electrophoresis after cutting both I-SceI sites. By this method, the precise location of these genes and the distance between gerB and narA were determined. This unique method is very useful and applicable as a biochemical tool for isolating any intended fragments, for blocking specific loci like site directed mutagenesis or large deletion, and for precise mapping of unknown genes by determination of their chromosomal distance and orientation.

Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.

Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3

<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>

Common prefrontal cortical gene expression profiles between adolescent SHR/NCrl and WKY/NCrl rats which showed inattention behavior

dela Peñ,a, Ike,Bang, Minji,Lee, Jinhee,de la Peñ,a, June Bryan,Kim, Bung-Nyun,Han, Doug Hyun,Noh, Minsoo,Shin, Chan Young,Cheong, Jae Hoon Elsevier 2015 Behavioural brain research Vol.291 No.-

<P><B>Abstract</B></P> <P>Factor analyses of attention-deficit/hyperactivity (ADHD) symptoms divide the behavioral symptoms of ADHD into two separate domains, one reflecting inattention and the other, a combination of hyperactivity and impulsivity. Identifying domain-specific genetic risk variants may aid in the discovery of specific biological risk factors for ADHD. In contrast with data available on genes involved in hyperactivity and impulsivity, there is limited information on the genetic influences of inattention. Transcriptional profiling analysis in animal models of disorders may provide an important tool to identify genetic involvement in behavioral phenotypes. To explore some of the potential genetic underpinnings of ADHD inattention, we examined common differentially expressed genes (DEGs) in the prefrontal cortex of SHR/NCrl, the most validated animal model of ADHD and WKY/NCrl, animal model of ADHD-inattentive type. In contrast with Wistar rats, strain representing the “normal” heterogeneous population, SHR/NCrl and WKY/NCrl showed inattention behavior in the Y-maze task. The common DEGs in the PFC of SHR/NCrl and WKY/NCrl vs. Wistar rats are those involved in transcription (e.g. <I>Creg1</I>, <I>Thrsp</I>, <I>Zeb2</I>), synaptic transmission (e.g. <I>Atp2b2</I>, <I>Syt12</I>, <I>Chrna5</I>), neurological system process (e.g. <I>Atg7</I>, <I>Cacnb4</I>, <I>Grin3a</I>), and immune response (e.g. <I>Atg7</I>, <I>Ip6k2</I>, <I>Mx2</I>). qRT-PCR analyses validated expression patterns of genes representing the major functional gene families among the DEGs (<I>Grin3a</I>, <I>Thrsp</I>, <I>Vof-16</I> and <I>Zeb2</I>). Although further studies are warranted, the present findings indicate novel genes associated with known functional pathways of relevance to ADHD which are assumed to play important roles in the etiology of ADHD-inattentive subtype.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We describe novel genes associated with inattention in SHR/NCrl and WKY/NCrl. </LI> <LI> These are genes involved in transcription, synapse transmission, immune system, etc. </LI> <LI> qRT-PCR validated expression patterns of <I>Grin3a, Zeb2, Vof-16</I> and <I>Thrsp</I>. </LI> <LI> Further studies are needed to determine their roles in ADHD inattentive subtype. </LI> </UL> </P>

비후된 심근에서의 유세포 분석을 통한 Nuclear DNA 의 관찰

김성진(Sung Jin Kim),방인숙(In Sook Bang),박언휘(Eun Hwi Park),김홍주(Heung Joo Kim),김도헌(De Heon Kim),이예봉(Ye Bong Lee),윤성철(Sung Chul Yun),한동선(Dong Sun Han),김성숙(Sung Sook Kim) 대한내과학회 1993 대한내과학회지 Vol.45 No.2

N/A Background: During the development of the cardiac hypertrophy, cytoplasmic contents of cardiac myocyte usually increase, but the changes in the nucleus of myocytes are not well understood. Therefore, we used flow cytometry to study the changes of nuclear DNA in cardiac hypertrophy from human autopsy heart tissue. Besides, we also tried to see the DNA ploidy of developing heart of fetus. Method: Nine hypertrophied human hearts ranging from 350-620 g (Group II: 350-500 g, Group III: > 500 g) and 9 control hearts (Group I: 250-350 g) which we could obtain after the legal autopsy were studied with flow cytometric analysis. And 3 fetal hearts of 20-24 gestational weeks (Group IV) were also studied. Nuclear DNA was analyzed with FACScan (Becton-Dickinson Co.) after tissue preparation using Modified Hedley and Vindelov Method and staining with Krishan staining buffer. Results: In control hearts (Group I), Flow cytometric analysis showed normal Diploidy pattern in all tissue. However, in cardiac hypertrophy, Non-Diploidy pattern was predominant (3 out of 5 specimen in Group II, and all 4 specimen in Group III) and Tetraploidy was usually observed in this abnormal ploidy pattern, but Aneuploidy was also seen in 2 cases of severe hypertrophy of Group III. Proliferative indexes increased progressively in Group I, II, and III (17.8±5.55, 24.8±7.4%, and 36.4±5.2%, respectively) (p<0.05 between Group I and II, and p<0.01 between Group I k III). Normal growing heart tissue of fetus showed normal Diploidy pattern. Conclusions: In cardiac hypertrophy, abnormal nuclear DNA ploidy pattern can be obseved. This finding might say that, during the genesis of cardiac hyper- trophy, not only cytoplasmic change but also DNA synthesis in the nucleus occurs, but G2 phase arrest happens without further mitosis.

Relationship between the methylenetetrahydrofolate reductase (MTHFR) rs1801133 SNP and serum homocysteine levels of Zhuang hypertensive patients in the central region of Guangxi

Hu Xi-Jiang,Su Mei-Ru,Cao Bao-Wei,Ou Fa-Bang,Yin Rui-Xing,Luo An-De 대한고혈압학회 2023 Clinical Hypertension Vol.29 No.-

Background The relationship between the methylenetetrahydrofolate reductase (MTHFR) single nucleotide polymorphism (SNP) and serum homocysteine (Hcy) levels or H-type hypertension in different populations is inconsistent. This study aimed to explore the association between the MTHFR rs1801133 SNP and serum Hcy levels of Zhuang hypertensive patients in the central region of Guangxi. Methods A total of 606 Zhuang inpatients with essential hypertension were recruited in our hospital from August 2016 to December 2018. The patients were divided into H-type hypertension (Hcy > 10 μmol/L, n = 528) and non–Htype hypertension (Hcy ≤ 10 μmol/L, n = 78) groups. At the same time, an age- and sex-matched group of 379 subjects with normal physical examination in our hospital were selected as the control group. Blood biochemical measurements and genotyping of the MTHFR rs1801133 SNP were performed. Results The prevalence of H-type hypertension was 87.13%. The levels of serum Hcy in patients with hypertension were higher than those in control group (14.20 ± 5.78 μmol/L vs. 11.97 ± 5.39 μmol/L, P < 0.001), especially in patients with H-type hypertension (15.08 ± 5.65 μmol/L, P < 0.001). The frequencies of TT genotype (22.73%) and T allele (46.21%) in patients with H-type hypertension were significantly higher than those in control group (11.35% and 30.47%, respectively) and non–H-type hypertension group (10.26% and 28.85%, respectively; P < 0.001 for all). Multivariate linear regression analysis showed that serum Hcy levels were significantly correlated with creatinine, lowdensity lipoprotein cholesterol, endogenous creatinine clearance rate, and the MTHFR rs1801133 genotypes in control group, while serum Hcy levels were significantly correlated with creatinine, triglyceride, low-density lipoprotein cholesterol, endogenous creatinine clearance rate, glycosylated hemoglobin, and the MTHFR rs1801133 genotypes in H-type hypertension group (P < 0.05–0.001). Serum Hcy levels in the T allele carriers were higher than those in the T allele noncarriers in both H-type hypertension and control groups. Conclusions There was closely related between the MTHFR rs1801133 SNP and serum Hcy levels in Zhuang patients with H-type hypertension in the central region of Guangxi. The MTHFR SNP may be an important reason for the increase of serum Hcy levels in Zhuang patients with H-type hypertension in this region.

Treatment Efficacy and Prognostic Factors for Huge HCC Based on Barcelona Clinic Liver Cancer Staging

Zhang, Zhi-Ming,Zhang, Yu-Mei,Gao, Sheng,Yuan, Wei-Ping,Zhao, Yin-Nong,Xiang, Bang-De,Wu, Fei-Xiang,Wu, Guo-Bin,Liu, Jian-Yong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Objective: To explore the most appropriate treatment for patients with hepatocellular cancer (HCC) >10 cm by using the Barcelona Clinic Liver Cancer (BCLC) classification. Materials and Methods: A total of 124 HCC patients undergoing surgery were selected. Disease-free survival (DFS), overall survival (OS) and prognostic factors were respectively assessed. Results: This study showed that the cumulative 1-, 3-, 5-year survival rates were 79.7%, 59.8% and 41.6% in BCLC-A patients, 76.2%, 9.5% and 0% in BCLC-B patients and 44.9%, 0% and 0% in BCLC-C patients, respectively. The 1-, 3-, 5-year DFS rates were 49%, 24.5% and 9.1% in BCLC-A patients, 7.5%, 0% and 0% in BCLC-B patients, respectively. No BCLC-C patients survived 1 year after surgery. Multivariate analysis indicated that hepatitis B surface antigen (HBsAg), vascular invasion, intra-hepatic metastasis, curative resection, tumor rupture and pathologic differentiation were independent prognostic factors. Conclusions: Surgery is effective and safe for patients with HCC >10 cm with sufficient hepatic reserve.