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Wheat Bran Globulins: Competitive Inhibitors of Mushroom Tyrosinase
Angel Martín Ortíz-Estrada,Jorge Nemesio Mercado-Ruiz,Alma Rosa Islas-Rubio,Ana María Mendoza-Wilson,René Renato Balandrán-Quintana,Jesús Manuel García-Robles 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.3
The inhibitory capacity of the globulin fraction of wheat bran against the diphenolase activity of mushroom tyrosinase, using L-tyrosine as substrate, was evaluated. Enzyme kinetics was monitored in the presence of globulin solutions by measuring the absorbance at 475nm. Lineweaver-Burk plots were drawn in order to determine Vmax, Km, and type of inhibition. Results showed that globulins from wheat bran competitively inhibited, the activity of mushroom tyrosinase with a KI of 0.79%(w/v). The degree of inhibition was 24% at 2 mM of the substrate L-tyrosine.