儒家思想과 書法藝術

沈伯綱 한양대학교 한국학연구소 1997 韓國學論集 Vol.30 No.-

Promoter demethylation mediates the expression of ZNF645, a novel cancer/testis gene

( Gang Bai ),( Yun Qiang Liu ),( Hao Zhang ),( Dan Su ),( Da Chang Tao ),( Yuan Yang ),( Yong Xin Ma ),( Si Zhong Zhang ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

Cancer/testis (CT) antigens exhibit highly tissue-restricted expression and are considered promising targets for cancer vaccines. Here we identified a novel CT gene ZNF645 which restrictively expresses in normal human testes and lung cancer patients (68.3%). To investigate the promoter methylation status of ZNF645, we carried out bisulfite genomic sequencing and found that the CpG island in its promoter was heavily methylated in normal lung tissues without the expression of ZNF645, whereas there was high demethylation in normal human testes and lung carcinoma tissues with its expression. Also ZNF645 could be remarkably activated in A549 and HEK293T cells treated by DNA demethylation agent 5`-aza-2`-deoxycytidine. And the dual luciferase assay revealed that the promoter activity of the ZNF645 was inhibited by methylation of the CpG island region. Therefore, we proposed that ZNF645 is a CT gene and activated in human testis and lung cancers by demethylation of its promoter region. [BMB reports 2010; 43(6): 400-406]

Villagers'Autonomy:Political Participation of Chinese Peasants

Bai Gang 신아시아연구소(구 신아세아질서연구회) 1997 신아세아 Vol.4 No.3

Normal Estimation for Mass Point Clouds of Irregular Model in the 3D Reconstruction based on Fuzzy Inference

Liu Yan-ju,Jiang Jin-gang,Miao Feng-juan,Tao Bai-rui,Zhang Hong-lie 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.5

This paper presents a fuzzy normal estimate for mass point clouds of irregular models in reconstruction. The irregular model is complex object that some part is smooth and some parts are irregular including sharp features. Therefore, we put kNN and curvature of mass point clouds to fuzzy inference system to divide the kind of point clouds and the output of FIS can determine which part of tooth point clouds belong to. For different kinds point clouds, corresponding algorithm is given. Point clouds in the smooth area are estimated normal by PCA directly and ones in other regions of thin or sharp area are estimated by checker and attach points. This method is simpler than those complex methods used on the whole point clouds directly. The experiment results show that much time is saved and surface reconstruction is very fine than PCA and WLOP.

Validation of Methods for Isolation and Culture of Alpaca Melanocytes: A Novel Tool for In vitro Studies of Mechanisms Controlling Coat Color

Bai, Rui,Sen, Aritro,Yu, Zhihui,Yang, Gang,Wang, Haidong,Fan, Ruiwen,Lv, Lihua,Lee, Kyung-Bon,Smith, George W,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

Predictive Impact of Common Variations in DNA Repair Genes on Clinical Outcome of Osteosarcoma

Bai, Sheng-Bin,Chen, Hong-Xiang,Bao, Yong-Xing,Luo, Xue-Gang,Zhong, Jin-Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

We aimed to assess the role of XPG, XPC and MMS19L polymorphisms on response to chemotherapy in osteosarcomas, and the clinical outcomes. One hundred and eighty five osteosarcoma patients who were histologically confirmed were enrolled in our study between January 2007 and December 2009. Genotyping of XPG, XPC and MMS19L was performed in a 384-well plate format on the MassARRAY$^{(R)}$ platform. Individuals with XPG TT genotype and T allele were more likely to be better response to chemotherapy than CC genotype, with the OR (95% CI) of 4.17 (1.64-11.54) and 2.66 (1.39-5.11), respectively. Those carrying MMS19L TT genotype and T allele showed better response to chemotherapy, with ORs (95% CI) of 4.8 (1.56-17.7) and 2.3 (1.22-4.36), respectively. Patients carrying TT genotype of XPG and MMS19L showed a significantly longer overall survival than CC genotype, with a 0.47 and 0.30-fold risk of death when compared with the wild-type of the gene. XPG and MMS19L are correlated with response to chemotherapy and prognosis of osteosarcoma, so that they could be used as predictive markers for prognosis.

Drilling off the Petrosal Apex and Opening the Upper Wall of Meckel's Cave Are the Key Elements of Good Outcomes in the Treatment of Trigeminal Neuralgia Secondary to Petrous Apex Meningioma

Bai, Jie,Zhou, Yufan,Song, Gang,Ren, Jian,Xiao, Xinru The Korean Neurosurgical Society 2022 Journal of Korean neurosurgical society Vol.65 No.3

Objective : The surgical management of trigeminal neuralgia (TN) caused by petrous apex meningioma (PAM) is still a challenge because of the lesion's deep location and the surrounding complex structures. The authors describe the intradural anterior transpetrosal approach (ATPA) and its effect on the treatment of TN secondary to PAM. Methods : A retrospective analysis of 15 patients with TN secondary to PAM who underwent surgery via the intradural ATPA was conducted. The key techniques, which included drilling off the petrosal apex (PA) and opening the upper wall of Meckel's cave (MC), are described in detail. Results : Total removal of the tumor and complete pain relief (Barrow Neurological Institute I) were achieved in all 15 patients without significant morbidity. Five patients developed new facial numbness postoperatively, which disappeared within three months after surgery. The postoperative magnetic resonance imaging showed temporal lobe swelling in three patients, but no clinical symptoms. One patient had cerebrospinal fluid leakage and was managed with bed rest and temporary lumbar drainage. One patient had an intracranial infection and was treated with antibiotics. By the last follow up, no patients had pain relapse or/and tumor recurrence. It is worth noting that the vascular compression at the root of the trigeminal nerve was found in one patient during the operation. Conclusion : Our experience suggests that drilling off the PA and opening the upper wall of the MC are key elements for a good outcome of the treatment of TN secondary to PAM. The intradural ATPA has the advantages for both tumor resection and pain relief.

Research on the Authentication of Radio Frequency Identification based on the Hash function

Bai Zhi,He Yi-Gang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.6

As a kind of accurate, rapid and real – time data acquisitions and processing technology, RFID can give unique identification to entity object, has been widely used in various industries, such as manufacturing, sales, transportation and so on. But with its widely application, many relevant problems, especially the safety issues of RFID system and the low cost issue of label have been raised more and more attention by people; therefore, a new and more appropriate security authentication protocol becomes necessary. In this paper, firstly introducing several now available security authentication protocols, and analyzing their strengths and drawbacks, then proposed a new authentication scheme based on Hash function, doing security properties and feasibility analysis of it in theory, and proving that the security properties of this scheme is more efficient, and it is more applicable to meet the needs of people through test at last.

Local Buckling of Steel Equal Angle Members with Normal and High Strengths

Gang Shi,Wenjing Zhou,Yu Bai,Zhao Liu 한국강구조학회 2014 International Journal of Steel Structures Vol.14 No.3

Steel angle sections have been widely accepted with the development of steel structures, and such members made by highstrength steel (HSS) sections are also increasingly used in buildings and bridges, and especially in transmission towers and longspan trusses. Compared to normal strength steels, HSS exhibits different mechanical properties, which can cause different localbuckling behavior. A finite element analysis (FEA) was performed in this paper to investigate the local buckling of steel equalangle members with different strengths under axial compression, where the residual stresses and the initial geometricimperfections of specimens were accurately described. Through this work, the relationship of the ultimate local buckling stressof steel equal angle members under axial compression as a function of steel strength and width-to-thickness ratio wasestablished. By comparing the FEA results with the international design specifications (ANSI/AISC 360-10 and Eurocode 3),a modified design formula was developed and corresponding design suggestions were proposed, to take into account the effectsof steel strength.

Delivery of SAV-siRNA via Exosomes from Adipose-Derived Stem Cells for the Treatment of Myocardial Infarction

Bai Weizhe,Zhu Tianchuan,Zuo Jiebin,Li Yang,Huang Xi,Li Gang 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.7

BACKGROUND: Myocardial infarction (MI) leads to cardiomyocyte death, poor cardiac remodeling, and heart failure, making it a major cause of mortality and morbidity. To restore cardiac pumping function, induction of cardiomyocyte regeneration has become a focus of academic interest. The Hippo pathway is known to regulate cardiomyocyte proliferation and heart size, and its inactivation allows adult cardiomyocytes to re-enter the cell cycle. METHODS: In this study, we investigated whether exosomes from adipose-derived stem cells (ADSCs) could effectively transfer siRNA for the Hippo pathway regulator Salvador (SAV) into cardiomyocytes to induce cardiomyocyte regeneration in a mouse model of MI. RESULTS: Our results showed that exosomes loaded with SAV-siRNA effectively transferred siRNA into cardiomyocytes and induced cardiomyocyte re-entry into the cell cycle, while retaining the previously demonstrated therapeutic efficacy of ADSC-derived exosomes to improve post-infarction cardiac function through anti-fibrotic, pro-angiogenic, and other effects. CONCLUSIONS: Our findings suggest that siRNA delivery via ADSC-derived exosomes may be a promising approach for the treatment of MI.