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Bai‐Zhong Zhang,Jun-Jie LIU,Xi-Ling CHEN,Guo-Hui YUAN 한국곤충학회 2018 Entomological Research Vol.48 No.5
In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real‐time reverse transcription polymerase chain reaction (RT‐qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including β‐actin (β‐ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde‐3‐phosphate (GAPDH), elongation fator‐alpha (EF1α), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha‐tubulin (α‐TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software‐based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web‐based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both RPL7/EF1α were suitable for experiments of different tissues, whereas for insecticide treatments, 28S/α‐TUB were suitable for normalizations of expression data. In addition, 28S/α‐TUB were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.
Xiliang Liu,Song Liu,Youkun Fan,Jin Qi,Xin Wang,Wei Bai,Dongliang Chen,Chengdong Xiong,Lifang Zhang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
The poly(L-lactide-co-e-caprolactone) (PLCL) ureteral stent creeps and loses shape stability, increasingthe risk of stent tube dislocation. The rubbery biodegradable cross-linked PLCL networks were preparedthrough gamma irradiation under vacuum in the presence of trimethylolpropane triacrylate (TMPTA),pentaerythritol tetraacrylate (PET4A), and pentaerythritol triacrylate (PETA). At a standard sterilizationdose of 25 kGy, the gel content and network density of PLCL networks increased with increasingcrosslinking agent content (1, 3, 5, 7 wt%), and crosslinking efficiency decreased in the order ofPETA > PET4A > TMPTA. The average molecular weight (Mc ) between two crosslinks ranged from 2000to 105 g/mol. To perform the beneficial semi-interpenetrated polymer network and characterized bythe principle, the networks were processed in several doses (25, 50, 75, 100, and 125 kGy). In place ofthe Charlesby-Pinner equation, the irradiation cross-linking followed the Chen-Liu-Tang equation. ThePLCL network with 7 wt% PETA had a gel fraction of 83%, tensile strength of 34.7 MPa, and tensile setvalue as low as 5%. Furthermore, degradation in vitro was slowed down. Thus, PLCL networks with appropriateelasticity and flexibility, inherent biodegradability, and excellent biocompatibility can provide apromising alternative method for soft tissue repair engineering, such as ureteral stents.
Radiative decay of theψ(2S)into two pseudoscalar mesons
Bai, J. Z.,Ban, Y.,Bian, J. G.,Blum, I.,Chen, A. D.,Chen, G. P.,Chen, H. F.,Chen, H. S.,Chen, J.,Chen, J. C.,Chen, X. D.,Chen, Y.,Chen, Y. B.,Cheng, B. S.,Choi, J. B.,Cui, X. Z.,Ding, H. L.,Dong, L. Y American Physical Society 2003 Physical review. D, Particles and fields Vol.67 No.3
Chen, Bai Hui,Park, Joon Ha,Ahn, Ji Hyeon,Cho, Jeong Hwi,Kim, In Hye,Lee, Jae Chul,Won, Moo-Ho,Lee, Choong-Hyun,Hwang, In Koo,Kim, Jong-Dai,Kang, Il Jun,Cho, Jun Hwi,Shin, Bich Na,Kim, Yang Hee,Lee, Y Medknow PublicationsMedia Pvt Ltd 2017 Neural regeneration research Vol.12 No.2
<P>Quercetin (QE; 3,5,7,3′,4′-pentahydroxyflavone), a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.</P>
Chen, Bai Hui,Park, Joon Ha,Cho, Jeong Hwi,Kim, In Hye,Shin, Bich Na,Ahn, Ji Hyeon,Hwang, Seok Joon,Yan, Bing Chun,Tae, Hyun Jin,Lee, Jae Chul,Bae, Eun Joo,Lee, Yun Lyul,Kim, Jong Dai,Won, Moo-Ho,Kang Medknow PublicationsMedia Pvt Ltd 2015 Neural regeneration research Vol.10 No.2
<P><I>Oenanthe javanica</I> is an aquatic perennial herb that belongs to the <I>Oenanthe genus</I> in Apiaceae family, and it displays well-known medicinal properties such as protective effects against glutamate-induced neurotoxicity. However, few studies regarding effects of <I>Oenanthe javanica</I> on neurogenesis in the brain have been reported. In this study, we examined the effects of a normal diet and a diet containing ethanol extract of <I>Oenanthe javanica</I> on cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus of adolescent rats using Ki-67 (an endogenous marker for cell proliferation) and doublecortin (a marker for neuroblast). Our results showed that <I>Oenanthe javanica</I> extract significantly increased the number of Ki-67-immunoreactive cells and doublecortin-immunoreactive neuroblasts in the subgranular zone of the dentate gyrus in the adolescent rats. In addition, the immunoreactivity of brain-derived neurotrophic factor was significantly increased in the dentate gyrus of the <I>Oenanthe javanica</I> extract-treated group compared with the control group. However, we did not find that vascular endothelial growth factor expression was increased in the <I>Oenanthe javanica</I> extract-treated group compared with the control group. These results indicate that <I>Oenanthe javanica</I> extract improves cell proliferation and neuroblast differentiation by increasing brain-derived neurotrophic factor immunoreactivity in the rat dentate gyrus.</P>
Chen Bai,Luo Mingqiang,Shen Zhong,Wu Zhe,Man Yiming,Fang Lei 한국항공우주학회 2014 International Journal of Aeronautical and Space Sc Vol.15 No.4
According to the requirement of wing weight estimation and frequent adjustments during aircraft conceptual design, a wing weight estimation method considering the constraints of structural strength and stiffness is proposed to help designers make wing weight estimations rapidly and accurately. This method implements weight predictions on the basis of structure weight optimization with stiffness constraints and strength constraints, which include achievement of wing shape parametric modeling, rapid structure layout, finite element (FE) model automated generation, load calculation, structure analysis, weight optimization, and weight computed based on modeling. A software tool is developed with this wing weight estimation method. This software can realize the whole process of wing weight estimation with the method and the workload of wing weight estimation is reduced because much of the work can be completed by the software. Finally, an example is given to illustrate that this weight estimation method is effective.
Bai, Chen,Mingqiang, Luo,Zhong, Shen,Zhe, Wu,Yiming, Man,Lei, Fang The Korean Society for Aeronautical and Space Scie 2014 International Journal of Aeronautical and Space Sc Vol.15 No.4
According to the requirement of wing weight estimation and frequent adjustments during aircraft conceptual design, a wing weight estimation method considering the constraints of structural strength and stiffness is proposed to help designers make wing weight estimations rapidly and accurately. This method implements weight predictions on the basis of structure weight optimization with stiffness constraints and strength constraints, which include achievement of wing shape parametric modeling, rapid structure layout, finite element (FE) model automated generation, load calculation, structure analysis, weight optimization, and weight computed based on modeling. A software tool is developed with this wing weight estimation method. This software can realize the whole process of wing weight estimation with the method and the workload of wing weight estimation is reduced because much of the work can be completed by the software. Finally, an example is given to illustrate that this weight estimation method is effective.