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Valerie Diane Valeriano,BAGON BERNADETTE,Marilen P. Balolong,Dae-Kyung Kang 한국미생물학회 2016 The journal of microbiology Vol.54 No.7
Bacterial lectins are carbohydrate-binding adhesins that recognize glycoreceptors in the gut mucus and epithelium of hosts. In this study, the contribution of lectin-like activities to adhesion of Lactobacillus mucosae LM1 and Lactobacillus johnsonii PF01, which were isolated from swine intestine, were compared to those of the commercial probiotic Lactobacillus rhamnosus GG. Both LM1 and PF01 strains have been reported to have good adhesion ability to crude intestinal mucus of pigs. To confirm this, we quantified their adhesion to porcine gastric mucin and intestinal porcine enterocytes isolated from the jejunum of piglets (IPEC-J2). In addition, we examined their carbohydrate-binding specificities by suspending bacterial cells in carbohydrate solutions prior to adhesion assays. We found that the selected carbohydrates affected the adherences of LM1 to IPEC-J2 cells and of LGG to mucin. In addition, compared to adhesion to IPEC-J2 cells, adhesion to mucin by both LM1 and LGG was characterized by enhanced specific recognition of glycoreceptor components such as galactose, mannose, and N-acetylglucosamine. Hydrophobic interactions might make a greater contribution to adhesion of PF01. A similar adhesin profile between a probiotic and a pathogen, suggest a correlation between shared pathogen–probiotic glycoreceptor recognition and the ability to exclude enteropathogens such as Escherichia coli K88 and Salmonella Typhimurium KCCM 40253. These findings extend our understanding of the mechanisms of the intestinal adhesion and pathogen-inhibition abilities of probiotic Lactobacillus strains.
Valeriano, Valerie Diane V.,Oh, Ju Kyoung,Bagon, Bernadette B.,Kim, Heebal,Kang, Dae-Kyung Elsevier 2019 Genomics Vol.111 No.1
<P><B>Abstract</B></P> <P> <I>Lactobacillus mucosae</I> is currently of interest as putative probiotics due to their metabolic capabilities and ability to colonize host mucosal niches. <I>L. mucosae</I> LM1 has been studied in its functions in cell adhesion and pathogen inhibition, <I>etc.</I> It demonstrated unique abilities to use energy from carbohydrate and non-carbohydrate sources. Due to these functions, we report the first complete genome sequence of an <I>L. mucosae</I> strain, <I>L</I>. <I>mucosae</I> LM1. Analysis of the pan-genome in comparison with closely-related <I>Lactobacillus</I> species identified a complete glycogen metabolism pathway, as well as folate biosynthesis, complementing previous proteomic data on the LM1 strain. It also revealed common and unique niche-adaptation genes among the various <I>L. mucosae</I> strains. The aim of this study was to derive genomic information that would reveal the probable mechanisms underlying the probiotic effect of <I>L. mucosae</I> LM1, and provide a better understanding of the nature of <I>L. mucosae</I> sp.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Comparative analysis of the complete genome of <I>L. mucosae</I> LM1 with those of other <I>L. mucosae</I> strains was performed. </LI> <LI> It identified complete glycogen metabolism and folate biosynthesis pathway, complementing previous proteomic data on LM1. </LI> <LI> It also revealed common and unique niche-adaptation genes among the various <I>L. mucosae</I> strains. </LI> </UL> </P>
Complete genome sequence of Lactobacillus plantarum SK151 isolated from kimchi
아모란토 미아,오주경,바곤 베르나데트,황인찬,김상훈,조준성,강대경,Amoranto, Mia Beatriz C.,Oh, Ju Kyoung,Bagon, Bernadette B.,Hwang, In-Chan,Kim, Sang Hoon,Cho, Chun-Sung,Kang, Dae-Kyung The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.3
Lactobacillus plantarum은 그람 양성, 비운동성의 이형발효세균이며, 다양한 환경에서 서식하고 있다. L. plantarum SK151은 장관상피세포 흡착능이 우수한 균주로서, 김치로부터 분리되었다. SK151 균주의 유전체 길이는 3,231,249 염기쌍이며, G + C 함량은 44.6%이었다. SK151 균주의 유전체에는 비타민 B2 (리보플라빈) 생합성에 필요한 유전자군이 완벽하게 존재하고 있었으며, 세포 흡착과 관련된 유전자도 다수 존재하고 있었다. Lactobacillus plantarum is a Gram-positive, facultative heterofermentative, nonspore-forming nonmotile bacterium found in a wide range of environmental niches. Here we present the complete genome sequence of L. plantarum SK151 isolated from kimchi, which shows high adhesion to intestinal epithelial cells. The genome is 3,231,249 bp in length and has a GC content of 44.6%. The genome contains genes related to cell adhesion and a complete operon for riboflavin biosynthesis.