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        Sugaring-out extraction of erythromycin from fermentation broth

        Sharayu Moharkar,Pradip Babanrao Dhamole 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.1

        This study reports the sugaring-out extraction of erythromycin from fermentation broth using acetonitrile (ACN) as solvent and glucose as a mass separating agent. Different process parameters--glucose concentration, temperature, ACN/water ratio and pH--were optimized to achieve maximum extraction of erythromycin. 88% (w/w) of erythromycin was extracted from the model system with following optimized conditions: glucose 156.3 g/L; temperature 4 oC; ACN/water ratio 1 and pH 8.3. Further, the effect of typical fermentation media components (starch, soybean flour, CaCO3, NaCl and (NH4)2SO4) on sugaring out extraction of erythromycin was also investigated. Starch, soybean flour and CaCO3 were observed to affect erythromycin extraction only at higher concentration. Removal of suspended solids from simulated as well as real broth prior to extraction enhanced the extraction efficiency (from 72% to 87%). Sugaring out extraction of erythromycin was found to be more effective than salting out extraction. Also, higher partition coefficient was achieved in the present work than other reported methods using carbohydrates as mass separating agent. Further, it was found that the antimicrobial activity of erythromycin was preserved during sugaring out extraction of erythromycin.

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        CO2 fixation and lipid production by microalgal species

        Srinivasa Reddy Ronda,Pavani Parupudi,Chandrika Kethineni,Pradip Babanrao Dhamole,Sandeep Vemula,Prasada Rao Allu,Mahendran Botlagunta,Sujana Kokilagadda 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2

        Microalgal species Nannochloropsis limnetica, Botryococcus braunii, and Stichococcus bacillaris were compared for their ability to grow, remove CO2, and accumulate lipids in their biomass under CO2-enriched atmosphere. Overall, N. limnetica outperformed the other two cultures and distinctly exhibited higher specific growth rate (0.999 d−1) and CO2 fixation rate (0.129 gL−1 d−1) with a high specific lipid yield (40% w/w). The volumetric CO2 fixation rate for all three species was validated with biomass productivity and mass transfer methods (P<0.005 and R2=0. 98). At 10% CO2, N. limnetica showed one-and-a-half times more carbon fixation efficiency over B. braunii, and S. bacillaris. On the other hand, total fatty acids of N. limnetica dispalyed an apparent increase in oleic acid. Whereas, under similar conditions, N. limnetica exhibited reduced eicosapentaenoic acid. These findings suggest that at high CO2 conditions, N. limnetica proved to be an efficient CO2 capture algal system and can be considered for biofuel applications.

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