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- 저자 : B.M.Murari (검색결과 1 건)
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N.K.Chaudhury,R.Bhardwaj,B.M.Murari 한국물리학회 2003 Current Applied Physics Vol.3 No.2, 3
Development of optical biosensors is an active area of research in the field of medical technology. Solgel matrices made fromalkoxide silicates,tetraethyl orthosilicate (TEOS) appear to be suitable glassy host matrix for the sensing system. However,themajor problem in the TEOS based solgel matrices is stability. So it is important to study dopantmatrix interaction as a function of time. In the present study,we report uorescence emission and excited state lifetime measurements on uorescent probes entrappedin TEOS solgel for monitoring the physico-chemical processes for characterization and monitoring of local environment (pores) ofviz.,Hoechst 33258 (H258) and pyranine (PY) were used. Solgels containing these probes were prepared at pH¼ 6:0 and thephysical and spectroscopic parameters were monitored as a function of storage time (days). The emission intensity from entrappedH258 has shown relatively higher extent of decrease during aging. The excited state uorescence lifetime measurements on theseprobes depicted single exponential decay component at 5.4 ns (PY) and 3.6 ns (H258) in fresh solgels. After a few days of storageprobe molecule PY. Further conrmation of multicomponents decay was obtained by distribution analysis of lifetime of H258where an increase in width of mean lifetime was observed with storage whereas no such change was indicated from PY. Thus itappears that H258 is a better probe molecule for characterizing and monitoring local environment of pores in solgel.. 2002 Elsevier Science B.V. All rights reserved.
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