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Gokarna, Anisha,Jun, Ki-Won,Khanna, P.K.,Baeg, Jin-Ook,Seok, Sang-Il Korean Chemical Society 2005 Bulletin of the Korean Chemical Society Vol.26 No.11
Formation of octahedral shaped PbSe quantum dots at higher synthesis temperature is being reported in this paper. The synthesis includes the reaction between lead oleate and trioctylphosphine selenide under inert gas conditions to produce PbSe. TEM, SEM, XRD and EDS were used to characterize the samples. The SEM exhibited the formation of spherical shaped nanocrystals at temperature below 140 ${^{\circ}C}$ and octahedral shaped nanoparticles at higher temperatures. Moreover, the TEM also showed the well resolved (111) lattice fringes proving that the nanocrystals were crystalline in nature. Synthesis of highly pure PbSe nanocrystals was another interesting aspect of this research.
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DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets
Shakya, Anisha,King, John T. Published for the Biophysical Society by the Rocke 2018 Biophysical journal Vol.115 No.10
<P><B>Abstract</B></P> <P>Phase separation of intracellular components has been recently realized as a mechanism by which cells achieve membraneless organization. Here, we study the associative liquid-liquid phase separation (LLPS) of DNA upon complexation with cationic polypeptides. Comparing the phase behavior of different single-stranded DNA as well as double-stranded DNA (dsDNA) sequences that differ in persistence lengths, we find that DNA local flexibility, not simply charge density, determines the LLPS. Furthermore, in a nucleotide- and DNA-dependent manner, free nucleotide triphosphates promote LLPS of polypeptide-dsDNA complexes that are otherwise prone to precipitation. Under these conditions, dsDNA undergoes a secondary phase separation forming liquid-crystalline subcompartments inside the droplets. These results point toward a role of local DNA flexibility, encoded in the sequence, in the regulation and selectivity of multicomponent LLPS in membraneless intracellular organization.</P>
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Mishra Anisha,Natanasabapathy Velmurugan,Suresh Nandini 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.4
Objective This study investigated the effects of various concentrations of sodium hypochlorite (NaOCl) on human whole-blood clotting kinetics, the structure of the blood clots formed, and transforming growth factor (TGF)-β1 release. Materials and Methods Human whole blood was collected from 5 healthy volunteers and divided into 4 groups: CG (control, 0.5 mL of blood), BN0.5 (0.5 mL of blood with 0.5 mL of 0.5% NaOCl), BN3 (0.5 mL of blood with 0.5 mL of 3% NaOCl), and BN5.25 (0.5 mL of blood with 0.5 mL of 5.25% NaOCl). The effects of NaOCl on clotting kinetics, structure of fibrin and cells, and release of TGF-β1 were assessed using thromboelastography (TEG), scanning electron microscopy (SEM), and enzyme-linked immunosobent assay, respectively. Statistical analysis was conducted using the Kruskal Wallis and Mann-Whitney U tests, followed by the post hoc Dunn test. A p value < 0.05 indicated statistical significance. Results The blood samples in BN0.5 and BN3 did not clot, whereas the TEG of BN5.25 showed altered clot formation. Samples from the CG and BN3 groups could only be processed with SEM, which showed that the latter lacked fibrin formation and branching of fibers, as well as clumping of red blood cells with surface roughening and distortion. TGF-β1 release was significantly highest in BN3 when all groups were compared to CG (p < 0.05). Conclusions Each concentration of NaOCl affected the release of TGF-β1 from blood clots and altered the clotting mechanism of blood by affecting clotting kinetics and cell structure. Objective This study investigated the effects of various concentrations of sodium hypochlorite (NaOCl) on human whole-blood clotting kinetics, the structure of the blood clots formed, and transforming growth factor (TGF)-β1 release. Materials and Methods Human whole blood was collected from 5 healthy volunteers and divided into 4 groups: CG (control, 0.5 mL of blood), BN0.5 (0.5 mL of blood with 0.5 mL of 0.5% NaOCl), BN3 (0.5 mL of blood with 0.5 mL of 3% NaOCl), and BN5.25 (0.5 mL of blood with 0.5 mL of 5.25% NaOCl). The effects of NaOCl on clotting kinetics, structure of fibrin and cells, and release of TGF-β1 were assessed using thromboelastography (TEG), scanning electron microscopy (SEM), and enzyme-linked immunosobent assay, respectively. Statistical analysis was conducted using the Kruskal Wallis and Mann-Whitney U tests, followed by the post hoc Dunn test. A p value < 0.05 indicated statistical significance. Results The blood samples in BN0.5 and BN3 did not clot, whereas the TEG of BN5.25 showed altered clot formation. Samples from the CG and BN3 groups could only be processed with SEM, which showed that the latter lacked fibrin formation and branching of fibers, as well as clumping of red blood cells with surface roughening and distortion. TGF-β1 release was significantly highest in BN3 when all groups were compared to CG (p < 0.05). Conclusions Each concentration of NaOCl affected the release of TGF-β1 from blood clots and altered the clotting mechanism of blood by affecting clotting kinetics and cell structure.
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전기원,Anisha Gokarna,P. K. Khanna,백진욱,석상일 대한화학회 2005 Bulletin of the Korean Chemical Society Vol.26 No.11
Formation of octahedral shaped PbSe quantum dots at higher synthesis temperature is being reported in this paper. The synthesis includes the reaction between lead oleate and trioctylphosphine selenide under inert gas conditions to produce PbSe. TEM, SEM, XRD and EDS were used to characterize the samples. The SEM exhibited the formation of spherical shaped nanocrystals at temperature below 140 oC and octahedral shaped nanoparticles at higher temperatures. Moreover, the TEM also showed the well resolved (111) lattice fringes proving that the nanocrystals were crystalline in nature. Synthesis of highly pure PbSe nanocrystals was another interesting aspect of this research.
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Fabrication of CdSe/ZnS Quantum-Dot-Conjugated Protein Microarrays and Nanoarrays
조용훈,Anisha Gokarna,이선균,황준석,임용택,정봉현 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.5
We demonstrate the fabrication and the detection of protein microarrays and nanoarrays, which are tagged by using colloidal CdSe/ZnS quantum dots (QDs), for the purpose of optical readout of those biochips. A microarrayer and a dip-pen nanolithography technique were used for the fabrication of QD-labeled protein microarrays and nanoarrays, respectively. The optical readout of those biochips was conducted by using a microchip scanner and a confocal scanning microscope in the case of the microarrays while an atomic force microscope was employed for characterizing the nanoarrays. We also report a possible way of reducing the ‘doughnut phenomenon’ in microarrays by varying the spot size from 300 µm to 100 µm, wherein the fluorescence intensity distribution becomes more uniform with decreasing spot diameter. We demonstrate the fabrication and the detection of protein microarrays and nanoarrays, which are tagged by using colloidal CdSe/ZnS quantum dots (QDs), for the purpose of optical readout of those biochips. A microarrayer and a dip-pen nanolithography technique were used for the fabrication of QD-labeled protein microarrays and nanoarrays, respectively. The optical readout of those biochips was conducted by using a microchip scanner and a confocal scanning microscope in the case of the microarrays while an atomic force microscope was employed for characterizing the nanoarrays. We also report a possible way of reducing the ‘doughnut phenomenon’ in microarrays by varying the spot size from 300 µm to 100 µm, wherein the fluorescence intensity distribution becomes more uniform with decreasing spot diameter.
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Roles of Power State and Message Types on Restaurant Store Brand Attitude
최낙환,Anisha Dhakal 한국유통과학회 2017 유통과학연구 Vol.15 No.10
Purpose – This research aims to find the moderation roles of power states in the effects of four message types (competence self-referencing, competence self-defining, warmth self-referencing, warmth self-defining) on brand attitude. Research design, data, and methodology – A restaurant brand was used as an experimental object, and 4(message types: warmth self-defining message, warmth self-referencing message, competence self-defining message, competence self-referencing message) x 2(power: high power and low power) between-subjects design was employed. Through on-line survey in Nepal, we collected a total of 240 individuals composed of eight experimental groups with 30 members in Nepal. Results – Consumers under low power state formed more positive brand attitude at the warmth self-defining message than any other types of message, while under high power condition, there are neither the attitude differences between competence self-referencing message and competence self-defining message, nor those between competence self-referencing message and warm self-defining message. The significant attitude differences showed between competence self-referencing message and warmth self-referencing message. Conclusions – This study contributes to the advertising theory development. Restaurant store marketers should deliver warmth self-defining message rather than the other three types of message to consumers under low power state, and they should not deliver warmth self-referencing message to consumers under high power state.
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S. Dhandayuthapani,K. Anisha 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.1
This efort endeavors on improving the dynamic time-response of a Wind Energy Conversion System associated to IEEE Thirty-Bus-System (TBS) using closed loop controlled Active Power Filter with FL-Controller. This efort deals with modeling & simulation of IEEE TBS employing FL-Controller based Shunt-active-flter. The SAF is executed with VoltageSource-Inverter & the switching-pulses are created utilizing FL-Controller. In this proposed work open loop IEEE TBS with and without SAF, closed loop PI and FLC based SAF are simulated and the corresponding time domain parameters are given. The outcome represents that FL-Controller system has better response than PI Controlled Shunt Active Filter system. The contribution of this work is to improve the dynamic response of SAF–TBS using FL -Controller.
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