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Naim, Amed 대한병원협회 1984 대한병원협회지 Vol.13 No.11
국민을 위한 복지제도의 운용에는 어느나라나 막론하고 지대한 관심과 이해가 교차되고 있는 듯 하다 나라마다 지향하는 정책방향이나 국가 재원에 의해 다소 차이는 있겠지마는, 국민 각자의 건강과 연계되는 의료복지 제도의 정착을 위한 보건비용조달체계는 제도운영의 묘와 함께 주요한 관심사라 하겠으며 나라마다 그 체재 또한 서로 상이할 것이다. 본 고는 이러한 견지에서 자연의 혜택으로 부를 누리는 "쿠웨이트"의 보건비용조달체제를 고찰하므로써 타국의 보건의료제도와 접해보는 기회를 갖고자 소개하는 바이다.
Confirmation of Green Fluorescent Protein Transformants using PCR
전선영 ( Sunyoung Jeon ),( Denver Walitang ),( Shamim Amed ),( Aritra Roy Choudhury ),사동민 ( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
Plant growth promoting bacteria (PGPB) have beneficial effects on plant growth via an enhancement of the nutrient status of their host plant. The means by PGPB enhance the nutrient status of host plants can be categorized into five areas: biological N2 fixation, increasing the availability of nutrients in the rhizosphere, inducing increases in root surface area, enhancing other beneficial symbioses of the host, and combination of modes of action. These bacteria have plant growth promoting traits such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase, indole acetic acid (IAA) production, salicyclic acid, phosphate solublization, siderophore production. In endophytic relationships, PGPB actually reside inside the host plant. PGPB maybe found in the surface of the root, stems or leaves. To find the location of PGPB in plant, Green Fluorescent Protein from jellyfish Aequoria victoria used as a valuable molecular marker which can be expressed in many organisms. GFP transformants emitting fluorescence were suitable for studying plant colonization under environmental condition. This study aims to assess the process of transformation of GFP insert into bacteria by PCR amplification. For the development of gfp mutant, triparental mating was carried out in Pseudomonas frederiksbergensis OB145, Pseudomonas umsongensis OB243, Pseudomonas umsongensis OB133, Pseudomonas moorei OR108, Pantoea sp CBMB55, Brevibacterium iodium RS16 and Burkholderia pyrrocinia CBPB-HOD using donor E.coli strain pFAJ1820 and helper E.coli strain pRK2013. Antibiotic resistance is used as a selection marker to ensure that bacteria in a culture contain a particular plasmid. Transfer of plasmid pFAJ1820 into these strains was confirmed through PCR amplification with specific primer. Band had been confirmed by using Gel-doc. In this study, plant growth promoting bacteria were successfully checked for GFP transformation. Confocal microscopy will be used to confirm effective colonization and intercellular localization of bacteria strains in plants.
Accumulation of High Levels of ABA Regulates the Pleiotropic Response of the nhr1 Arabidopsis Mutant
Francisco Quiroz-Figueroa,Adrián Rodríguez-Acosta,Amed Salazar-Blas,Elizabeta Hernández-Domínguez,Maria Eugenia Campos,Nobutaka Kitahata,Tadao Asami,Rosa M. Galaz-Avalos,Gladys I. Cassab 한국식물학회 2010 Journal of Plant Biology Vol.53 No.1
Plants have evolved a variety of mechanisms for responding to environmental cues, which allows them to survive in the presence of limited resources or environmental stresses. One of the most significant growth adaptations plants have attained is tropism, a growth response that involves bending of plant organs toward or away from a stimulus. Roots exhibit hydrotropism in response to moisture gradients, which is thought to be critical in acquiring water and establishing their stand in the soil. However, the mechanism underlying hydrotropism remains unsolved. Here, we report that the no hydrotropic response (nhr1) mutant of Arabidopsis, which is impaired in hydrotropism, is tolerant to drought. The no hydrotropic response phenotype of nhr1 was repressed by AbamineSG,an inhibitor of abscisic acid (ABA) biosynthesis, indicating that ABA negatively regulates hydrotropism. Furthermore,the content of ABA was higher in nhr1 compared to those of wild type (wt). However, the higher ABA levels in nhr1plants were not due to higher transcript levels of 9-cisepoxycarotenoid dioxygenase (NCED3), since these were diminished compared to those of wt. Our results indicated that the root hydrotropic response of the nhr1 mutant is modulated by ABA and that the higher ABA levels of the mutant might confer it drought resistance.
Ameli, Fereshteh,Rose, Isa Mohd,Masir, Noraidah Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6
Background: Invasive ductal (IDC) and lobular (ILC) carcinomas are the common histological types of breast carcinoma which are difficult to distinguish when poorly differentiated. Discoidin domain receptor (DDR1) and Drosophila dishevelled protein (DVL1) were recently suggested to differentiate IDC from ILC. Objectives: To assess the expression of DDR1 and DVL1 and their association with histological type, grading and hormonal status of IDC and ILC. Materials and Methods: This cross sectional study was conducted on IDC and ILC breast tumours. Tumours were immunohistochemically stained for (DDR1) and (DVL1) as well as estrogen receptor (ER), progesterone receptor (PR) and C-erbB2 receptor. Demographic data including age and ethnicity were obtained from patient records. Results: A total of 51 cases (30 IDCs and 21 ILCs) were assessed. DDR1 and DVL1 expression was not significantly associated with histological type (p=0.57 and p=0.66 respectively). There was no association between DDR1 and DVL1 expression and tumour grade (p=0.32 and p=1.00 respectively), ER (p=0.62 and 0.50 respectively), PR (p=0.38 and p=0.63 respectively) and C-erbB2 expression (p=0.19 and p=0.33 respectively) in IDC. There was no association between DDR1 and DVL1 expression and tumour grade (p=0.52 and p=0.33 respectively), ER (p=0.06 and p=0.76 respectively), PR (p=0.61 and p=0.43 respectively) and C-erbB2 expression (p=0.58 and p=0.76 respectively) in ILC. Conclusions: This study revealed that DDR1 and DVL1 are present in both IDC and ILC regardless of the tumour differentiation. More studies are needed to assess the potential of these two proteins in distinguishing IDC from ILC in breast tumours.
Aming Wang,Feng Liu,Liangjun Xia,Chunhua Zhang,Sijie Zhou,Zhuan Fu,Yunli Wang,Weilin Xu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.103 No.-
Severe effluent pollution and chemical damage to protein fibers generated from conventional aqueousdyeing process have stimulated interest in exploiting sustainable dyeing of wool. Herein, an ethanol(EtOH)-carbon tetrachloride (CCl4)-water (H2O) ternary solvent system was used as the dyeing mediumto protect wool fibers from being damaged in the dyeing process. The wool fibers were firstly pretreatedin an aqueous acid solution, followed by dyeing in the EtOH-CCl4-H2O (45:50:5) ternary solvent systemwith reactive dyes. The results indicated that the acid pretreatment solution could be reused for severaltimes, the samples dyed in the EtOH-CCl4-H2O mixture with the investigated dyes showed higher K/S valuescompared to the conventional approach, implying the increased dye utilization and decreased effluentsdischarge were realized. Furthermore, acceptable permeability, good colorfastness, and satisfactoryleveling properties of the dyed samples were achieved using the developed method. Additionally, thesolvent-assisted dyed samples showed improved mechanical properties compared with the conventionallydyed samples. The dyeing mechanisms of wool fibers in the EtOH-CCl4-H2O mixture were illustratedin detail. The proposed dyeing technology consumes fewer energy, freshwater, and chemicals comparedwith the conventional wool dyeing method. Future work will principally concentrate on the reuse of thedye-contaminated EtOH-CCl4-H2O mixture.
Augmenting Seismic Data via Generative Adversarial Network for Low-cost MEMS Sensors
Aming Wu,Jae-Kwang Ahn,Young-Woo Kwon 한국통신학회 2021 한국통신학회 학술대회논문집 Vol.2021 No.6
The deep learning (DL) model’s performance is related to algorithm design and depends on sufficient training data set. The lack of real seismic data set and many data polluted by dif-ferent types of noise are the key factors that restrict the breakthrough in seismology research based on machine learning approaches. Therefore, data generation technic is fundamental for data set augmentation. Because it is difficult to perceive the spatial-temporal correlation and evolution process of seismic sequences, the intelligent generation of seismic sequences is still a significant challenge. A novel deep generation model (DGM) based on a generative adversarial network (GAN) is developed to generate high-quality seismic data. The experimental results show that the model can generate realistic seismic data through autocorrelation analy-sis, error quantitative index analysis, and other evaluation methods, and the model’s accuracy is over 80%.
Ameli Forough,Ameli Forough,Zokaee Ashtiani Farzin 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.5
This paper discusses a novel approach for exploring the aggregation mechanism of bovine serum albumin using collision factor. The population balance equation consisting of aggregation term was developed and solved by the moment method. Different experiments were implemented to account for effective parameters on protein aggregation and to measure variations in average size of aggregates formed in a time interval. This was done by taking pictures with a CCD camera on a sterio microscope. The pictures were exported to image processing software to analyze average number and size of aggregates. The collision factor appearing in population balance equation was optimized and declared as a global term.