Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

Alessandra Bernadete Trovo De Marqui,Alessandra Vidotto,Giovana Mussi Polachini,Claudia De Mattos Bellato,Hamilton Cabral,Andreia Machado Leopoldino,Jose Francisco De Gois Filho,Erica Erina Fukuyama,F 한국생활환경학회 2006 BMB Reports Vol.39 No.2

Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

De Marqui, Alessandra Bernadete Trovo,Vidotto, Alessandra,Polachini, Giovana Mussi,De Mattos Bellato, Claudia,Cabral, Hamilton,Leopoldino, Andreia Machado,De Gois Filho, Jose Francisco,Fukuyama, Erica Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.2

In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.

Comparison between fiber-reinforced polymers and stainless steel orthodontic retainers

Alessandra Lucchese,Maurizio Manuelli,Claudio Ciuffreda,Paolo Albertini,Enrico Gherlone,Letizia Perillo 대한치과교정학회 2018 대한치과교정학회지 Vol.48 No.2

Objective: The aim of this study was to examine the properties of fiberreinforced composite and stainless steel twisted retainers for orthodontic retention. Methods: Three different span lengths (5.0, 8.0, and 14.0 mm) of fiber-reinforced composite were investigated. The three fiber-reinforced composite retainer groups were subdivided according to the storage condition (dry and wet), resulting in a total of six groups. Each stainless steel and fiberreinforced composite group was comprised of six specimens. The threepoint bending flexural test was conducted using a universal testing machine. ANOVA was used to assess differences in the maximum load and maximum stress according to the span length, material, and storage condition. Post-hoc comparisons were performed if necessary. Results: The maximum stress and maximum load were significantly (p < 0.001) associated with the span length, material, and storage condition. The significant interaction between the material and span length (p < 0.001) indicated the differential effects of the material for each span length on the maximum stress and maximum load, with the difference between materials being the highest for the maximum span length. Conclusions: Our findings suggest that fiber-reinforced composite retainers may be an effective alternative for orthodontic retention in patients with esthetic concerns or allergy to conventional stainless steel wires.

Robotic Gastrectomy: The Current State of the Art

Alessandra Marano,형우진 대한위암학회 2012 Journal of gastric cancer Vol.12 No.2

Since the first laparoscopic gastrectomy for cancer was reported in 1994, minimally invasive surgery is enjoying its wide acceptance. Numerous procedures of this approach have developed, and many patients have benefited from its effectiveness, which has been recently demonstrated for early gastric cancer. However, since laparoscopic surgery is not exempt from some limitations, the robotic surgery system was introduced as a solution by the late 1990's. Many experienced surgeons have embraced this new emerging method that provides undoubted technical and minimally invasive advantages. To date, several studies have concentrated to this new system, and have compared it with open and laparoscopic approach. Most of them have reported satisfactory results concerning the post-operative short-term outcomes, but almost all believe that the role of robotic gastrectomy is still out of focus, especially because long-term outcomes that can prove robotic oncologic equivalency are lacking, and operative costs and time are higher in comparison to the open and laparoscopic ones. This article is a review about the current status of robotic surgery for the treatment of gastric cancer, especially, focusing on the technical aspects, comparisons to other approaches and future prospects.

Recurrent Optic Neuritis as the Initial Symptom in Demyelinating Diseases

Alessandra Billi,Falcão-Gonçalves,Denis Bernardi Bichuetti,Enedina Maria Lobato de Oliveira 대한신경과학회 2018 Journal of Clinical Neurology Vol.14 No.3

Background and Purpose Optic neuritis (ON) is an inflammation of the optic nerve that can be recurrent, with unilateral or bilateral presentation. Diagnosing recurrent cases may be challenging. We aimed to compare patients with recurrent ON as their initial symptom according to their following final diagnoses: multiple sclerosis (MS), neuromyelitis optica spectrum disorders (NMOSD), or chronic relapsing inflammatory optic neuropathy (CRION). Methods The medical records of patients with initial recurrent ON who were followed at the Neuroimmunology Clinic of the Federal University of São Paulo between 2004 and 2016 were analyzed retrospectively. Patients were classified according to their final diagnosis into MS, NMOSD, or CRION, and the characteristics of these groups were compared to identify predictive factors. Results Thirty-three patients with recurrent ON were included, and 6, 14, and 13 had final diagnoses of MS, NMOSD, and CRION, respectively. Most of the patients were female with unilateral and severe ON in their first episode, and the initial Visual Functional System Score (VFSS) was ≥5 in 63.6%, 85.7%, and 16.7% of the patients with CRION, NMOSD, and MS, respectively. Anti-aquaporin-4 antibodies were detected in 9 of 21 (42.8%) tested patients. Seven of nine (77.8%) seropositive NMOSD patients experienced transverse myelitis episodes during the follow-up period. A multivariate regression analysis showed that the VFSS at the last medical appointment predicted the final diagnosis. Conclusions A lower VFSS at the last medical appointment was predictive of MS. Patients with NMOSD and CRION have similar clinical characteristics, whereas NMOSD patients tend to have worse visual acuity.

Robotic versus Laparoscopic versus Open Gastrectomy: A Meta-Analysis

Alessandra Marano,형우진,최윤영,김유민,김지은,노성훈 대한위암학회 2013 Journal of gastric cancer Vol.13 No.3

Purpose: To define the role of robotic gastrectomy for the treatment of gastric cancer, the present systematic review with meta-analysis was performed. Materials and Methods: A comprehensive search up to July 2012 was conducted on PubMed, EMBASE, and the Cochrane Library. All eligible studies comparing robotic gastrectomy versus laparoscopic gastrectomy or open gastrectomy were included. Results: Included in our meta-analysis were seven studies of 1,967 patients that compared robotic (n=404) with open (n=718) or laparoscopic (n=845) gastrectomy. In the complete analysis, a shorter hospital stay was noted with robotic gastrectomy than with open gastrectomy (weighted mean difference: -2.92, 95% confidence interval: -4.94 to -0.89, P=0.005). Additionally, there was a significant reduction in intraoperative blood loss with robotic gastrectomy compared with laparoscopic gastrectomy (weighted mean difference:-35.53, 95% confidence interval: -66.98 to -4.09, P=0.03). These advantages were at the price of a significantly prolonged operative time for both robotic gastrectomy versus laparoscopic gastrectomy (weighted mean difference: 63.70, 95% confidence interval: 44.22to 83.17, P<0.00001) and robotic gastrectomy versus open gastrectomy (weighted mean difference: 95.83, 95% confidence interval:54.48 to 137.18, P<0.00001). Analysis of the number of lymph nodes retrieved and overall complication rates revealed that these outcomes did not differ significantly between the groups. Conclusions: Robotic gastrectomy for gastric cancer reduces intraoperative blood loss and the postoperative hospital length of stay compared with laparoscopic gastrectomy and open gastrectomy at a cost of a longer operating time. Robotic gastrectomy also provides an oncologically adequate lymphadenectomy. Additional high-quality prospective studies are recommended to better evaluate both short and long-term outcomes.

Resveratrol Promotes Self-digestion to Put Cancer to Sleep

Alessandra Ferraresi,Ciro Isidoro,Suyanee Thongchot 대한암예방학회 2024 Journal of cancer prevention Vol.29 No.1

Resveratrol, a natural polyphenol present in a variety of food stuff, has been shown to exert preventive and curative anticancer activity in several in vitro and in vivo models. Such chemopreventive/anticancer activity has been linked to biochemical and epigenetic modifications of multiple pathways involved in carcinogenesis and metastasization. In this commentary, we focus on the recent work done in our laboratory showing that resveratrol has potential to prevent and cure cancer by promoting epigenetic-mediated autophagy-dependent tumor dormancy, an effect associated with re-education of the cancer-associated fibroblasts and reduced production of inflammatory cytokines in the tumor microenvironment. The clinical translation of the current knowledge on resveratrol nticancer activity is also discussed.

Structural Optimization of Hollow-section Steel Trusses by Differential Evolution Algorithm

Alessandra Fiore,Giuseppe Carlo Marano,Rita Greco,Erika Mastromarino 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.2

This paper deals with the weight minimization of planar steel trusses by adopting a differential evolution-based algorithm. Square hollow sections are considered. The design optimization refers to size, shape and topology. The design variables are represented by the geometrical dimensions of the cross sections of the different components of the truss, directly involving the size of the structure, and by some geometrical parameters affecting the outer shape of the truss. The topology is included in the optimization search in a particular way, since the designer at different runs of the algorithm can change the number of bays keeping constant the total length of the truss, to successively choose the best optimal solution. The minimum weight optimum design is posed as a single-objective optimization problem subject to constraints formulated in accordance with the current Eurocode 3. The optimal solution is obtained by a Differential Evolutionary (DE) algorithm. In the DE algorithm, a particular combination of mutation and crossover operators is adopted in order to achieve the best solutions and a specific way for dealing with constraints is introduced. The effectiveness of the proposed approach is shown with reference to two case-studies. The analysis results prove the versatility of the optimizer algorithm with regard to the three optimization categories of sizing, shape, topology as well as its high computational performances and its efficacy for practical applications. In particular useful practical indications concerning the geometrical dimensions of the various involved structural elements can be deduced by the optimal solutions: in a truss girder the cross section of the top chord should be bigger than the one of the bottom chord as well as diagonals should be characterized by smaller cross sections with respect to the top and bottom chords in order to simultaneously optimize the weight and ensure an optimal structural behaviour.

Robotic Intraoperative Tracheobronchial Repair during Minimally Invasive 3-Stage Esophagectomy

Alessandra Marano,Silvia Palagi,Luca Pellegrino,Felice Borghi 대한흉부외과학회 2021 Journal of Chest Surgery (J Chest Surg) Vol.54 No.2

Tracheobronchial injury (TBI) is an uncommon but potentially fatal event. Iatrogenic le- sions during bronchoscopy, endotracheal intubation, or thoracic surgery are considered the most common causes of TBI. When TBI is detected during surgery, concomitant sur- gical treatment is recommended. Herein we present a case of successful robotic primary repair of iatrogenic tracheal and left bronchial branch tears during a robot-assisted hybrid 3-stage esophagectomy after neoadjuvant chemoradiotherapy. A robotic approach can facilitate the repair of this injury while reducing both the potential risk of conversion to open surgery and the associated increased risk of postoperative respiratory complications.

Effect of Drag Stages Surface Roughness on the Compression Ratio of a TMDP

Alessandra Dal Bianco,Luca Bonmassar 한국진공학회 2016 Applied Science and Convergence Technology Vol.25 No.6

The rotor of a turbomolecular drag pump is generally made of an aluminum alloy. Its surface finish is affected by various processes that the rotor itself undergoes during the manufacturing phase. The impact of different surface finishes on the pumping performances of a turbomolecular pump has been mainly investigated by Sawada et al [1]. The present work aims to broaden the previous bibliographic study to the drag stages of a turbomolecular pump by testing the impact of different surface finishes on the compression ratio of the pump. Experimental tests have been made focusing on two processes: the corundum sandblasting and the glass microspheres shot-peening. Both the processes flatten and/or physically remove EDM melted spheres; in particular, blasted surfaces obtained by glass shotpeening are generally smoother than surfaces obtained by corundum sandblasting. In order to characterize the surface texture left by such processes, preliminary surface roughness measurements have been made on the drag rotor disks of several pumps. The experimental tests conducted on both sandblasted and shot-peened rotors confirms previous results obtained on the turbo stages by Sawada et al. [1], showing that the average roughness of the surface has an impact on the compression ratio of the pump; in particular, an increment in the surface roughness causes a corresponding increment in the compression ratio of the pump and vice versa. For the tested pumps, the higher surface roughness gives a factor of increment of about 2 on the measured hydrogen maximum compression ratio of the pump.