Genome-wide identification and analysis of Subtilisin-like serine protease gene family in banana (Musa accuminta L.) and their expression under abiotic stresses

Purwar Shalini,Chugh Vishal,Singh Poornima,Srivastava Akhilesh Kumar,Singh Akhilesh Kumar,Mishra Akhilesh Chandra,Singh Ashutosh,Singh Chandra Mohan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Subtilisin-like serine proteases (SBT), a serine proteolytic enzymes play an important role in plant growth function and during different stresses responses. The systematic analysis of the SBT gene family in Musa acuminate (MaSBT) has been done and their responses to abiotic stresses in banana variety cv. G-9 were also analyzed. Total of 67 MaSBT genes were identified and based on phylogeny these were grouped into five districted subgroups. Cis-acting element analysis indicated that almost all MaSBT promoters contain regulatory elementary related to growth and development, hormonal regulation, and stress responses. The gene structure and domain analysis showed a maximum of seventeen exons and four functional domains in MaSBT. The 42 orthologous genes, 07 MaSBT paralogous genes were also identified through synteny analysis. The Ka /Ks study indicated that four MaSBT paralogous gene pairs were tandemly duplicated, while the other three were segmental duplications. Further, the expression pattern via RNA-seq data revealed that MaSBTs exhibited differential expres- sion specifically in response to the abiotic stress of low nitrogen. and also during the flowering time. The MaSBT-1.7 gene was found involved in the response to salt stress and flowering. These findings establish a cornerstone for future research on banana's salt stress mechanism. The study offers valuable insights into SBT encoding genes, shedding light on their roles in growth, development, and abiotic stress responses

Preparation and Magnetic Properties of a Neutral Octadecanuclear Manganese Complex [MnII 4MnIII 14(O)14(O2CMe)18(hmp)4(hmpH)2(H2O)2]

Akhilesh K. Gupta,윤석원,나명수,김진권 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.6

Flexural ductility of RC beam sections at high strain rates

Akhilesh K. Pandey 사단법인 한국계산역학회 2013 Computers and Concrete, An International Journal Vol.12 No.4

Computation of flexural ductility of reinforced concrete beam sections has been proposed by taking into account strain rate sensitive constitutive behavior of concrete and steel, confinement of core concrete and degradation of cover concrete during load reversal under earthquake loading. The estimate of flexural ductility of reinforced concrete rectangular sections has been made for a wide range of tension and compression steel ratios for confined and unconfined concrete at a strain rate varying from 3.3 × 10-5 to 1.0/sec encountered during normal and earthquake loading. The parametric studies indicated that flexural ductility factor decreases at increasing strain rates. Percentage decrease is more for a richer mix concrete with the similar reinforcement. The confinement effect has marked influence on flexural ductility and increase in ductility is more than twice for confined concrete (0.6 percent volumetric ratio of transverse steel) compared to unconfined concrete. The provisions in various codes for achieving ductility in moment resisting frames have been discussed.

Ethnic food culture of Chhattisgarh state of India

Akhilesh Shukla 한국식품연구원 2021 Journal of Ethnic Foods Vol.8 No.-

Chhattisgarh state is situated in the central part of India and is also known as the ‘Bowl of rice’. Paddy is the principal crop of this state, and rice is the staple diet of the people. The state largely maintains its ethnic food culture as most of its population continues to live within rural and tribal areas. The state has nearly 44% of forest cover which serves as a decent source of food. People here prefer a vegetarian diet, and over 70 varieties of leaves, 25 varieties of tubers and roots are used here as vegetables. The present article is an attempt to explore the ethnic food culture of Chhattisgarh and to provide information about rice and non-rice-based traditional recipes, sweet dishes, leafy vegetables, tubers and roots which are consumed here. Irrespective of all the modern changes in food habits, the people of Chhattisgarh still preserve their traditional food culture.

Incorporation of Whole Spine Screening in Magnetic Resonance Imaging Protocols for Low Back Pain: A Valuable Addition

Akhilesh Rao,Atul Mishra,Yayati Pimpalwar,Ravinder Sahdev,Neha Yadu 대한척추외과학회 2017 Asian Spine Journal Vol.11 No.5

Study Design: A retrospective review of lumbar magnetic resonance imaging (MRI) studies conducted at the Department of Radiodiagnosis & Imaging of a Tertiary Care Armed Forces Hospital between May 2014 and May 2016. Purpose: To assess the advantages of incorporating sagittal screening of the whole spine in protocols for conventional lumbar spine MRI for patients presenting with low back pain Overview of Literature: Advances in MRI have resulted in faster examinations, particularly for patients with low back pain. The additional detection of incidental abnormalities on MRI helps to improve patient outcomes by providing a swifter definitive diagnosis. Because low back pain is extremely common, any change to the diagnostic and treatment approach has a significant impact on health care resources. Methods: We documented all additional incidental findings detected on sagittal screenings of the spine that were of clinical significance and would otherwise have been undiagnosed. Results: A total of 1,837 patients who met our inclusion criteria underwent MRI of the lumbar spine. The mean age of the study population was 45.7 years; 66.8% were men and 33.2% women. Approximately 26.7% of the patients were diagnosed with incidental findings. These included determining the level of indeterminate vertebrae, incidental findings of space-occupying lesions of the cervicothoracic spine, myelomalacic changes, and compression fractures at cervicothoracic levels. Conclusions: We propose that T2-weighted sagittal screening of the whole spine be included as a routine sequence when imaging the lumbosacral spine for suspected degenerative pathology of the intervertebral discs.

Influence of frictional packing limit on hydrodynamics and performance of gas-solid fluidized beds

Akhilesh Kumar Sahu,Vasudevan Raghavan,Bhamidi Prasad 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.12

The influence of frictional packing limit (FPL) on prediction of hydrodynamics and performance of fluidized bed reactors was studied. Dense gas-solid flows in non-reactive (under isothermal cold and at elevated temperatures) and reactive atmospheres (fluidized bed gasifier) were simulated using Eulerian-Eulerian methodology considering a range of values for FPL. Simulations under cold flow conditions were conducted to establish a range of FPL values that provides physically realistic predictions. It is noticed that bed pressure drop increases with increasing value of FPL when superficial gas velocity (U) is less than or equal to the minimum fluidization velocity. For larger values of U, predicted pressure drop is unaffected by the choice of value of FPL. However, in these cases, the distribution of particles, their velocities and bubbling behavior are significantly affected by FPL. Effect of FPL at elevated temperatures is similar to the one observed at cold flow conditions. It is further noticed that FPL not only affects the predictions on bed hydrodynamics but also has profound influence on reactive flow characteristics such as bed temperature and product gas composition. Sensitivity analysis under cold flow conditions could reveal better predictions when the ratio of FPL to close packing limit is chosen between 0.9 and 0.97.

Efficacy of Sweet Potato Powder and Added Water as Fat Replacer on the Quality Attributes of Low-fat Pork Patties

Akhilesh K. Verma,Manish Kumar Chatli,Devendra Kumar,Pavan Kumar,Nitin Mehta 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.2

The present study was conducted to investigate the efficacy of sweet potato powder (SPP) and water as a fat replacer in low-fat pork patties. Low-fat pork patties were developed by replacing the added fat with combinations of SPP and chilled water. Three different levels of SPP/chilled water viz. 0.5/9.5% (T-1), 1.0/9.0% (T-2), and 1.5/8.5% (T-3) were compared with a control containing 10% animal fat. The quality of low-fat pork patties was evaluated for physico-chemical (pH, emulsion stability, cooking yield, aw), proximate, instrumental colour and textural profile, and sensory attributes. The cooking yield and emulsion stability improved (p<0.05) in all treatments over the control and were highest in T-2. Instrumental texture profile attributes and hardness decreased, whereas cohesiveness increased compared with control, irrespective of SPP level. Dimensional parameters (% gain in height and % decrease in diameter) were better maintained during cooking in the low-fat product than control. The sensory quality attributes juiciness, texture and overall acceptability of T-2 and T-3 were (p<0.05) higher than control. Results concluded that low-fat pork patties with acceptable sensory attributes, improved cooking yield and textural attributes can be successfully developed with the incorporation of a combination of 1.0% SPP and 9.0% chilled water.

Preparation and Magnetic Properties of a Neutral Octadecanuclear Manganese Complex [Mn^II₄Mn^III₁₄(O)₁₄(O₂CMe)₁₈(hmp)₄(hmpH)₂(H₂O)₂]

Gupta, Akhilesh K.,Yoon, Seok-Won,Lah, Myoung-Soo,Kim, Jin-Kwon Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.6

Demographic Risk Factors, Affected Anatomical Sites and Clinicopathological Profile for Oral Squamous Cell Carcinoma in a North Indian Population

Krishna, Akhilesh,Singh, R.K.,Singh, Shraddha,Verma, Pratima,Pal, U.S.,Tiwari, Sunita Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Oral cancer is a common form of cancer in India, particularly among men. About 95% are squamous cell carcinomas. Tobacco along with alcohol are regarded as the major risk factors. Objectives: (i) To determine associations of oral squamous cell carcinoma (OSCC) with respect to gender, age group, socioeconomic status and risk habits; (ii) To observe the distribution of affected oral anatomical sites and clinico-pathological profile in OSCC patients. Materials and Methods: This is an unmatched case-control study during period January 2012 to December 2013. Total of 471 confirmed OSCC patients and 556 control subjects were enrolled. Data on socio-demography, risk habits with duration and medical history were recorded. Results: There were significant associations between OSCC with middle age (41-50years; unadjusted OR=1.63, 95%CI=1.05-2.52, p=0.02) (51-60 years; unadjusted OR=1.79, 95%CI=1.15-2.79, p=0.009) and male subjects (unadjusted OR=2.49, 95%CI=1.89-3.27, p=0.0001). Cases with both habits of tobacco chewing and smoking were at a higher risk for OSCC than tobacco chewing alone (unadjusted OR=0.52, 95%CI=0.38-0.72, p=0.0001), duration of risk habits also emerged as a responsible factor for the development of carcinoma. The majority of patients were presented in well-differentiated carcinomas (39.9%). Prevalence of advance stages (TNM stage III, IV) was 23.4% and 18.3% respectively. The buccal mucosa was the most common (35.5%) affected oral site. Conclusions: In most Asian countries, especially India, there is an important need to initiate the national level public awareness programs to control and prevent oral cancer by screening for early diagnosis and support a tobacco free environment.

Coumarin-based combined computational study to design novel drugs against Candida albicans

Maurya Akhilesh Kumar,Mishra Nidhi 한국미생물학회 2022 The journal of microbiology Vol.60 No.12

Candida species cause the most prevalent fungal illness, candidiasis. Candida albicans is known to cause bloodstream infections. This species is a commensal bacterium, but it can cause hospital–acquired diseases, particularly in COVID-19 patients with impaired immune systems. Candida infections have increased in patients with acute respiratory distress syndrome. Coumarins are both naturally occurring and synthetically produced. In this study, the biological activity of 40 coumarin derivatives was used to create a three-dimensional quantitative structure activity relationship (3D-QSAR) model. The training and test minimum inhibitory concentration values of C. albicans active compounds were split, and a regression model based on statistical data was established. This model served as a foundation for the creation of coumarin derivative QSARs. This is a unique way to create new therapeutic compounds for various ailments. We constructed novel structural coumarin derivatives using the derived QSAR model, and the models were confirmed using molecular docking and molecular dynamics simulation.