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- 저자 : Acharjee (검색결과 6 건)
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Effect of Thidiazuron (TDZ) on In vitro Regeneration of Blackgram (Vigna mungo L.) Embryonic Axes
Acharjee, Sumita,Handique, Pratap Jyoti,Sarmah, Bidyut Kumar 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.4
Regeneration has been achieved in blackgram (Vigna mungo) using thidiazuron (TDZ) in the culture medium. The explanted cotyledon with wounded embryonic axes produced the highest number (9.75-10.45) of healthy, elongated shoots when cultured on shoot bud regeneration medium (SRI) composed of 2 ${\mu}M$ BAP, 2 ${\mu}M$ KIN, 2 ${\mu}M$ TDZ, and 0.5 ${\mu}M$ NAA followed by multiple shoot regeneration (SRII) medium containing 2 ${\mu}M$ BAP, 2 ${\mu}M$ KIN, and multiple shoot elongation (SE) medium (0.5 ${\mu}M$ of BAP + 0.5 ${\mu}M$ of KIN). The presence of TDZ in combination with BAP and NAA in the SRI medium for one sub-culture cycle (10 - 14 days) significantly increases formation of multiple shoot buds per explant. Independent, healthy shoots obtained were selected for both in vitro rooting and grafting. Establishment of plantlets in the soil was highest (80 - 100%) in the case of in vitro rooted compared to grafted shoots (40%). The protocol appears to be competent to Agrobacterium-meditated transformation with 'gus' as a reporter gene. PCR analysis of the $T_0$ and $T_1$ progenies showed the presence and transmission of the transgene. We document here the regeneration and transformation of blackgram using cotyledons with wounded embryonic axes and the protocol appears to be suitable for genetic transformation of blackgram.
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Mobility-Aware Node Clustering with Fuzzy Logic for Wireless Mesh Network
Tapodhir Acharjee,Sudipta Roy 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.12
In recent times, Wireless Mesh Network (WMN) has received prominence worldwide due to the evolution of wireless networks as a ubiquitous and seamless broadband service provider. Routing on WMNs is one of the most prominent research issues on these days. Hierarchical clustering based routing protocols are proposed by the researchers for large networks. In these protocols, all the available nodes are grouped into clusters. Each clus-ter consists of cluster members, cluster head and gateway nodes where cluster head plays the most vital role to form an efficient cluster. Selection of a better cluster head depends on the decisions made from different parameters and their relations. In this situation, fuzzy logic suits better by providing better analysis and helps to make the right decision based on different parameter values and their relations. In this paper, a fuzzy logic based hierarchical clustering technique is proposed for WMN. The proposed technique along with the existing Fuzzy Logic Control Clustering Algorithm(FLCCA) are experimented using the simulation software NS3 and the simulated results are compared to establish the efficiency of the proposed technique.
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Effect of Thidiazuron (TDZ) on In vitro Regeneration of Blackgram (Vigna mungo L.) Embryonic Axes
Sumita Acharjee,Pratap Jyoti Handique,Kumar Sarmah 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.4
Regeneration has been achieved in blackgram (Vigna mungo) using thidiazuron (TDZ) in the culture medium. The explanted cotyledon with wounded embryonic axes produced the highest number (9.75-10.45) of healthy, elongated shoots when cultured on shoot bud regeneration medium (SRI) composed of 2 μM BAP, 2 μM KIN, 2 μM TDZ, and 0.5 μM NAA followed by multiple shoot regeneration (SRII) medium containing 2 μM BAP, 2 μM KIN, and multiple shoot elongation (SE) medium (0.5 μM of BAP + 0.5μM of KIN). The presence of TDZ in combination with BAP and NAA in the SRI medium for one sub-culture cycle (10 - 14 days)significantly increases formation of multiple shoot buds per explant. Independent, healthy shoots obtained were selected for both in vitro rooting and grafting. Establishment of plantlets in the soil was highest (80 - 100%) in the case of in vitro rooted compared to grafted shoots (40%). The protocol appears to be competent to Agrobacterium-meditated transformation with ‘gus’ as a reporter gene. PCR analysis of the T0 and T1 progenies showed the presence and transmission of the transgene. We document here the regeneration and transformation of blackgram using cotyledons with wounded embryonic axes and the protocol appears to be suitable for genetic transformation of blackgram.
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Sujata Acharjee,Kaushik Maiti,Jae Mok Soh,Wook-Bin Im,Jae Young Seong,Hyuk Bang Kwon 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1
We previously demonstrated the presence of three dis-tinct types of the gonadotropin-releasing hormone re-ceptor (GnRHR) in a bullfrog (denoted bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). The bfGnRHRs exhib-ited differential tissue distribution and ligand selectiv-ity. In the present study, we demonstrated the desensi-tization and internalization kinetics of these receptors in both transiently-transfected HEK293 cells and ret-rovirus-mediated stable cells. The time-course accu-mulation of the inositol phosphate in response to GnRH revealed that bfGnRHR-1 and -2 were rapidly desensitized, whereas bfGnRHR-3 was slowly desensi-tized. A comparison of the internalization kinetics re-vealed the most rapid rate and highest extent of inter-nalization of bfGnRHR-2 among the three receptors. Interestingly, the mechanisms that underlie the recep-tor internalization appear to differ from each other. Internalization of bfGnRHR-1 was dependent on both dynamin and b-arrestin, whereas those of bfGnRHR-2 and -3 were dependent on dynamin, but not on b-arrestin. These results, therefore, suggest that differen-tial regulatory mechanisms for desensitization and internalization of the GnRHR are involved in diverse cellular and physiological responses to GnRH stimula-tion.
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Jae Young Seong,Kaushik Maiti,Jian Hua Li,Ai Fen Wang,Sujata Acharjee,Wang Phil Kim,임욱빈,권혁방 한국분자세포생물학회 2003 Molecules and cells Vol.16 No.2
ecently, we identified three types of non-mammalian gonadotropin-releasing hormone receptors (GnRHR) in the bullfrog (designated bfGnRHR-1-3), and a mammalian type-II GnRHR in green monkey cell lines (denoted gmGnRHR-2). All these receptors responded better to GnRH-II than GnRH-I, while mammalian type-I GnRHR showed greater sensitivity to GnRH-I than GnRH-II. In the present study, we designed new GnRH-II analogs and examined whether they acti- vated or inhibited non-mammalian and mammalian type-II GnRHRs. [D-Ala 6 ]GnRH-II, with D-Ala substi- tuted for Gly 6 in GnRH-II, increased inositol phos- phate (IP) production in cells stably expressing non- mammalian GnRHRs more effectively than native GnRH-II. However, it exhibited lower activity for mammalian type-I GnRHR than GnRH-I itself. Trptorelix-1, a GnRH-II antagonist, inhibited GnRH- induced IP production in cells expressing non- mammalian GnRHRs more effectively than Cetrorelix, a GnRH-I antagonist. Trptorelix-1, however, had lower potency for mammalian type-I GnRHR than Cetrorelix. Ligand-receptor binding assays revealed that [D-Ala 6 ]GnRH-II and Trptorelix-1 have higher affinities for non-mammalian GnRHRs but lower af- finities for mammalian type-I GnRHR than GnRH-II and Cetrorelix, respectively. Moreover, [D-Ala 6 ]GnRH- II and Trptorelix-1 had a higher affinity for gmGnRHR-2 than GnRH-II and Cetrorelix, respec- tively. These results indicate that [D-Ala 6 ]GnRH-II and Trptorelix-1 are highly effective agonist and antagonist, respectively, for non-mammalian and type- II mammalian GnRHRs
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