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Abo-Amer Aly E.,Gad El-Rab Sanaa M. F.,Halawani Eman M.,Niaz Ameen M.,Bamaga Mohammed S. 한국미생물·생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.12
Staphylococcus aureus is a cause of high mortality in humans and therefore it is necessary to prevent its transmission and reduce infections. Our goals in this research were to investigate the frequency of methicillin-resistant S. aureus (MRSA) in Taif, Saudi Arabia, and assess the relationship between the phenotypic antimicrobial sensitivity patterns and the genes responsible for resistance. In addition, we examined the antimicrobial efficiency and application of silver nanoparticles (AgNPs) against MRSA isolates. Seventy-two nasal swabs were taken from patients; MRSA was cultivated on Mannitol Salt Agar supplemented with methicillin, and 16S rRNA sequencing was conducted in addition to morphological and biochemical identification. Specific resistance genes such as ermAC, aacA-aphD, tetKM, vatABC and mecA were PCR-amplified and resistance plasmids were also investigated. The MRSA incidence was ~49 % among the 72 S. aureus isolates and all MRSA strains were resistant to oxacillin, penicillin, and cefoxitin. However, vancomycin, linezolid, teicoplanin, mupirocin, and rifampicin were effective against 100% of MRSA strains. About 61% of MRSA strains exhibited multidrug resistance and were resistant to 3-12 antimicrobial medications (MDR). Methicillin resistance gene mecA was presented in all MDR-MRSA strains. Most MDR-MRSA contained a plasmid of > 10 kb. To overcome bacterial resistance, AgNPs were applied and displayed high antimicrobial activity and synergistic effect with penicillin. Our findings may help establish programs to control bacterial spread in communities as AgNPs appeared to exert a synergistic effect with penicillin to control bacterial resistance.
Abo-Mosallam, H.A.,Kim, D.A.,Kim, H.W.,Lee, H.H. Ceramurgica ; Elsevier Science Ltd 2016 Ceramics international Vol.42 No.4
The impact of ZrO<SUB>2</SUB> content of the glass on the formation, properties and crystallization of glass ionomer cements (GICs) was investigated. Glass series based on SiO<SUB>2</SUB>-Al<SUB>2</SUB>O<SUB>3</SUB>-ZrO<SUB>2</SUB>-P<SUB>2</SUB>O<SUB>5</SUB>-CaO-CaF<SUB>2</SUB> system was synthesized and studied. The cements were characterized using a setting time, flexural strength, fracture toughness and in vitro biocompatibility test. The setting time of the ionomer cement increased with increasing the ZrO<SUB>2</SUB> content of the glass. The cements showed a slight decrease of cell biocompatibility with increase the ZrO<SUB>2</SUB> oxide content in the glasses. The results also showed that the flexural strength and the fracture toughness of the cements increased with immersion time and ZrO<SUB>2</SUB> oxide content. The crystallization characteristics of the glasses were investigated by differential scanning calorimeter (DSC) and X-ray diffraction analysis (XRD). The addition of ZrO<SUB>2</SUB> oxide in the glasses led to increase both the glass transition and crystallization temperatures. Fluorapatite [Ca<SUB>5</SUB>(PO<SUB>4</SUB>)<SUB>3</SUB>F], mullite [Al<SUB>6</SUB>Si<SUB>2</SUB>O<SUB>13</SUB>], cristobalite [SiO<SUB>2</SUB>] and zircon [ZrSiO<SUB>4</SUB>] phases were crystallized from the investigated glasses. The role played by the glass oxide constituents in determining the setting time, mechanical properties and crystallization characteristics of the prepared glass ionomer was discussed.
Abo-Elyousr, A.M. Kamal,Sallam, M.A.A.,Hassan, M.H.A.,Zeller, W. The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.1
The effect of Acibenzolar-S-methyl (ASM) and Rahnella aquatilis Ra39 against apple fire blight disease caused by Erwinia amylovora were tested as a possible alternative to streptomycin. In vitro studies, no inhibition effect against the pathogen was found when ASM was tested. Under greenhouse conditions, application of R. aquatilis Ra39 with the highly susceptible M26 rootstock resulted in a marked disease suppression. Application of ASM and strain Ra39 caused a high decrease of the disease, 82% and 58% respectively; this was correlated with a reduction of the growth of the pathogen within host plants up to 64% and 49.5% respectively. Further studies in the field under artificial infection condition during full bloom revealed that application of ASM and R. aquatilis Ra39 with Gala variety resulted in a control effect up to 21 and 29% respectively. In physiological studies, enhanced activities of PR-proteins (chitinase and $\beta$-1, 3-glucanase) were detected, which are well known as biochemical markers for systemic acquired resistance. Application of ASM to apple shoots caused the highest chitinase activity followed by strain Ra39. The enzyme activity was increased after 2, 4 and 6 days from application. In addition, ASM-treatment caused the higher $\beta$-1, 3-glucanase activity than strain Ra39. Maximum enzyme activity was recorded after 6 days from application and then decreased after 8 and 10 days from application.
Abo-Zeid Salim Maha Ahmed,Bakrey Sherine,Elbadrawy Rania Elmohamady 대한마취통증의학회 2023 Korean Journal of Anesthesiology Vol.76 No.4
Background: The shivering effect after spinal anesthesia in total knee arthroplasty (TKA) is challenging for anesthesiologists. This study aimed to compare two administration routes of dexmedetomidine as a post-neuraxial shivering prevention measure and an adjunctive analgesic and sedative agent.Methods: Fifty-six patients were randomly allocated into two equal groups. The intravenous dexmedetomidine (IV dex) group received an IV infusion of 0.5 µg/kg dexmedetomidine diluted in 20 ml saline and an adductor canal block (ACB) consisting of 20 ml of 0.25% levobupivacaine and 1 ml saline. The adductor canal block dexmedetomidine (ACB dex) group received a 20 ml IV infusion of saline and an ACB consisting of 20 ml 0.25% levobupivacaine and 1 ml of 0.5 µg/kg dexmedetomidine.Results: The incidence of shivering 1 h post spinal anesthesia was equal in both groups (50%); however, the shivering grade was significantly lower in the IV dex group 1 h postoperatively. The onset of sensory block was significantly later in the IV dex group (22.14 ± 2.52 min) than in the ACB dex group (12 ± 3.31 min). Postoperative analgesic duration (h) was significantly longer in the ACB dex group (12.28 ± 4.47) compared to the IV dex group (9.28 ± 1.90). The sedation scores were also significantly higher in the IV dex group in the preoperative, intraoperative, and immediate postoperative periods.Conclusions: While perineural ACB dexmedetomidine had similar intraoperative anti-shivering with less sedative effects as IV dexmedetomidine, it was associated with both less shivering control and superior analgesia post-TKA under spinal anesthesia.
Abo-Elyousr A. M. Kamal,Hussein M. A. Mohamed,Allam A. D. Aly,Hassan A. H. Mohamed 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.2
In this study, we investigated the induced defense response and protective effects against Stemphylium vesicarium by application of benzothiadiazole (Bion®) and di-potassium phosphate salt (K2HPO4) to onion. Onion leaves were sprayed with Bion® and K2HPO4, then inoculated 2 days later with a virulent strain of S. vesicarium under greenhouse conditions. Disease severity and activities of peroxidase (PO), polyphenoloxidase, phenylalanine ammonia-lyase (PAL) and phenol contents were evaluated in the treated leaf tissues. Reduction in the disease severity was observed in plants treated with Bion® and K2HPO4. Onion plants treated with Bion® and K2HPO4 and inoculated with the pathogen showed significantly higher PAL activity, PO activity, and phenol contents than inoculated water-treated plants 2 days after the treatment. In conclusion, the results of this study provide evidence that application of simple non-toxic chemical solutions as di-potassium phosphate and Bion® can control Stemphylium leaf blight of onion.
Management of Tomato Root-knot Nematode Meloidogyne incognita by Plant Extracts and Essential Oils
Abo-Elyousr, Kamal A.M.,Awad, Magd El-Morsi,Gaid, M.A. Abdel The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.2
The effect of plant extracts of eucalyptus (Eucalyptus chamadulonsis), garlic (Allium sativium), marigold (Tagetes erecta) and neem (Azadirachta indica) and essential oils were tested on the suppression of root-knot nematode Meloidogyne incognita under greenhouse and field conditions. In vitro study, all tested treatments had nematicidal effect on nematode juveniles after 24 and 48 hours from exposures. The highest percentage of nematode mortality was achieved by application of neem extract (65.4%), essential oils (64.4%) and marigold extract (60.5%), followed by garlic and eucalyptus extracts (38.7-39.5%). Under greenhouse and field conditions, neem extract and essential oils treatments were more effective in reducing population numbers of the M. incognita in soil and root gall index compared to other treatments. In field experiments, the maximum protection of tomato plant against root-knot nematode was obtained by application of neem and essential oil treatments, 44.2 and 32.6%, respectively.
Abo-Elyousr, Kamal A.M.,Mohamed, Hashem M. The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.2
Three plant growth-promoting yeasts and two rhizobacteria were tested for controlling tomato wilt caused by Fusarium oxysporum f. sp. lycopersici under green-house and field conditions. Under greenhouse and field conditions, all treatments were significantly reduced disease severity of tomato wilt relative to the infected control. The highest disease reductions in pots (75.0, 67.4%) and field (52.5, 42.4%) were achieved by Azospirillum brasilense and Bacillus subtilis compared to infected control. Under field condition all treatments produced the highest tomato yield compared to the control plants inoculated with the pathogen.
GLOBAL ASYMPTOTIC STABILITY OF A SECOND ORDER RATIONAL DIFFERENCE EQUATION
Abo-Zeid, R. The Korean Society for Computational and Applied M 2010 Journal of applied mathematics & informatics Vol.28 No.3
The aim of this paper is to investigate the global stability, periodic nature, oscillation and the boundedness of solutions of the difference equation $x_{n+1}\;=\;\frac{A+Bx_{n-1}}{C+Dx_n^2}$, n = 0, 1, 2, ... where A, B are nonnegative real numbers and C, D > 0.