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Agata Wasilewska,Katarzyna Ponanta-Gawron,Beata Burtan,Beata Burtan,Charissa Stephen Chandra Sagaran,Mariusz Duplaga,Kinga Kowalska-Duplaga 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.4
Pediatric Crohn's disease (CD) is commonly associated with hepatobiliary complications, including transient liver enzymes elevation (LEE). Exclusive enteral nutrition (EEN) is recommended as primary treatment in mild-to-moderate pediatric CD. Data concerning EEN and liver enzymes (LE) abnormalities are limited. The aim of this study was to assess the LEE occurrence in newly diagnosed CD pediatric patients during EEN. Retrospective analysis of 73 patients, with no previous signs of liver disease, qualified to EEN. LE were assessed at diagnosis, during EEN, after completion of the nutritional treatment, and reintroduction of free diet. Thirty-one (42%) children presented with LEE and 28 (38%) with transient LEE. The LEE cohort presented with higher percentage of protein energy (24.0% ± 29.4 IQR [interquartile range] vs. 18.6% ± 23.6 IQR, P < .05) versus nonprotein energy (fat and carbohydrates) in total energy intake (75.9% ± 29.4 IQR vs. 81.4% ± 23.6 IQR, P < .05). Also, the protein/energy ratio was higher in the LEE group compared with the group with normal LE (0.026 vs. 0.024, P = .028). At the fourth week of EEN, aspartate aminotransferase elevation correlated with higher daily protein intake (P < .018). The LEE during EEN is typically a low-grade and transient condition that may be connected to applied treatment. We hypothesize that higher protein/energy ratio during EEN may be associated with mild, temporary LEE. Careful observation with repeated measurement of LE activity may be sufficient proceeding in patients without any other symptoms of CD-associated liver disease.
Health Risk Impact from 131I Exposure to Low or High Doses Evaluated with Micronucleus Assay
Antonina Cebulska-Wasilewska,Justyna Miszczyk,Zbigniew Drag,김진규 한국방사선산업학회 2011 방사선산업학회지 Vol.5 No.4
Despite the fact that the precautions to be taken are clearly established, accidentalexposures from various radiation sources, including nuclear power plants, in which emerge of the131I among emission of various radioactive isotopes, might be one of the first sign of misfortune,that even if infrequently, continue to occur. In human body, iodine, is preferentially concentratingin the thyroid, though, it is frequently used in nuclear medicine both diagnostically and therapeutically. The aim of our study was to investigate the influence of radioiodine-131, from the diagnosticand therapeutic treatment exposures, on cellular DNA repair efficiency and various biomarkersof effects, in order to estimate a potentially associated health risk at low and high dose regions. Study group consisted of 41 individuals diagnostically exposed to low dose of the 131I (in the range1.85~4.45 MBq, A´sr=2.96±0.82 MBq) and 37 persons therapeutically exposed to high dose ofthe 131I (in the range 300~650 MBq, A´sr=497.3±88.1 MBq). A reference group consisted of 30healthy individuals. Blood samples that were collected for the investigations underwent immediatelycytogenetic procedures according to standard protocols for retrospective biological dosimetry. Strong variation between cellular responses of thyroid diseases patients to both low and highdoses of 131I is observed, although, mean value of cytogenetic damage is in the group investigatedafter diagnostic treatment lower than in control. Nevertheless, five weeks after the therapeuticdose of 131I, in majority of patients significantly elevated levels of cytogenetic damage are observed,that suggest a possible increase of secondary cancer risk. Suppression of detected level ofcytogenetic damage observed for some individuals in a high dose region might suggest limitationof this biomarker for retrospective biological dosimetry in the wide range of doses or necessity ofinvestigation of personalised susceptibility.
Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression
Krupnik, Tomasz,Wasilewska, Wioleta,Drozak, Anna,Romanowska, Elzbieta,Zienkiewicz, Maksymilian The Korean Society of Phycology 2018 ALGAE Vol.33 No.4
The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliable promoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed by protein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolae chloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknown aspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of useful chemicals.
Application of chloroplast promoters of Cyanidioschyzon merolae for exogenous protein expression
Tomasz Krupnik,Wioleta Wasilewska,Anna Drożak,Elżbieta Romanowska,Maksymilian Zienkiewicz 한국조류학회I 2018 ALGAE Vol.33 No.4
The ability to transform the chloroplast of Cyanidioschyzon merolae was limited by lack of confirmed and reliablepromoter sequences (among other reasons), capable of delivering stable or modulated DNA transcription followed byprotein synthesis. Our research has confirmed the applicability of three selected chloroplast promoters in C. merolaechloroplast overexpression of the exogenous protein (i.e., chloramphenicol acetyltransferase) and genetic transformation. These results might facilitate further research on genetically modified strains of C. merolae to envisage yet unknownaspect of cellular and plastic physiology as well as C. merolae potential applications as bio-factories or sources of usefulchemicals.
Effect of Superoxide Dismutase and Low Molecular Mediators on Lignin Degradation
Cho, Nam Seok,Leonowicz, Andrzej,Matuszewska, Anna,Luterek, Jolanta,Ziegenhagen, Dirk,Wasilewska, Maria Wojtas,Hofrichter, Martin,Rogalski, Jerzy 한국목재공학회 1999 목재공학 Vol.27 No.4
As the biodegradation of wood constituents has been understood as a multi-basidiomycetes and enrymatic processes, this review will focus on the roles of low molecular compounds and radicals working in harmony with fungal enzymes. Wood rotting basidiomycete fungi penetrate wood, and lead to more easily metabolize carbohydrates of the wood complex. The white-rot fungi, having versatile enzymes, are able to attack directly the $quot;lignin barrier$quot;. They also use a multi-enzyme system including so-called $quot;feedback$quot; type enzymes allowing for simultaneous degradation of lignin and carbohydrates. The multi-enzymes including laccase support the proposed route by explaining how the high molecular weight enzymes can function in the wood complex. These enrymes may function separately or cooperate each other. In addition, veratryl alcohol oxidase, cellobiose dehydrogenase, arylalcohol dehydrogenase, and particularly low molecular mediators and radicals have an important role in wood biodegradation. However, the possibility of other mechanism as well as other enzymes, as operating as feedback systems in the process of wood degradation, could not be excluded.
자주달개비 수술털에서 방사선에 의해 유발되는 분홍돌연변이에 대한 광주기의 영향
김진규(Jin Kyu Kim),김원록(Won Rok Kim),(A . Cebulska Wasilewska) 한국환경생물학회 1999 환경생물 : 환경생물학회지 Vol.17 No.3
The present study was carried out to investigate the combined effect of radiation and photoperiod (PP) regimes on Tradescantia 4430 somatic cell mutations. Potted plants were irradiated with 0.3, 0.5 and 1.0 Gy of gamma radiation from ^60Co source. The plants irradiated only with gamma radiation were used as control group (CT). The somatic cell mutation rate in 0.5 Gy irradiated CT and PP20 group started to increase on the 6th day and reached a maximum value on the 10th day and 9th day after irradiation while the rate in the experimental group under 4 hours of photoperiod a day (PP4) started to increase on the 10th day and reached a maximal value on the 16th day post-irradiation. The slope of dose-response curve in CT was 5.99 (r^2=0.99), while it was 6.93 (r^2=0.98) in PP20 and 11.74 (r^2=0.99) in PP4, respectively. The biological efficacy of radiation in the induction of pink mutation increased by 15.7% in PP20 and 95.9% in PP4, respectively. It is suggested that photoperiod regimes unfavorable to the plant have an additive effect on radiation-induced mutations and a delaying or inhibiting effect on cell damage repair, as well. [photoperiod, pink mutation, radiation, Tradescantia].