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다이아몬드전착 밴드쏘우장비를 이용한 고치밀도 알루미나소결체의 다이싱가공 성능평가
이용문(Yong-Moon Lee),박영찬(Young-Chan Park),김동현(Dong-Hyun Kim),이만영(Man-Young Lee),강명창(Myung Chang Kang) 한국기계가공학회 2017 한국기계가공학회지 Vol.16 No.6
Recently, the brittle materials such as ceramics, glass, sapphire and textile material have been widely used in semiconductors, aerospace and automobile owing to high functional characteristics. On the other hand, it has the characteristics of difficult-to-cut material relative to all materials. In this study, diamond electro-deposited band-saw machine was developed to operate stably using water-coolant type through relative motion between band-saw tool and Al₂O₃ material. High densified Al₂O₃ material was manufactured by spark plasma sintering method. The bulk density was observed by the Archimedes law and the theoretical density was estimated to be 3.88 g/cm³ and its hardness 14.7 MPa. From the dicing sawing test of Al₂O₃ specimen, behavior of surface roughness and band-saw wear are dominantly affected by the increase of the band-saw linear velocity. Additionally, an continuous pattern type of diamond band-saw was a very effective due to entry impact as a one-off for brittle material.
초음파 탐촉자(Probes)의 위생관리 실태와 감염 인식도 조사 및 세균 오염도 측정
김창복(Chang Bok Lee),이양섭(Yang Sub Lee),이원홍(Won Hong Lee),조정찬(Cheong Chan Cho),윤향이(Hyang Yi Yoon),이용문(Yong Moon Lee),김영근(Young Keun Kim),이경섭(Kyung Sup Lee) 대한방사선과학회(구 대한방사선기술학회) 2004 방사선기술과학 Vol.27 No.3
The gel, which is stained on probe after ultrasonogmphy, is a good circumstances for proliferation of microbe. This study is to investigate into the actual state of sanitary management, recognition degree and infection level of ultrasonographic probes. We had performed a question with telephone to 42 hospitals in Seoul area from December in 2003. We also cultured to obtained a sample from three ultrasonographic units to investigate infection level of the probes. Sanita교 management of the probes was performed in 21 hospitals with alcohol cotton, Sanitary management was performed daily in 14 hospitals. Most hospitals used cotton towel for clearing of gel stained on probes. Preventive management against infection was performed in 32 hospitals with vinyl cover, surgical glove, or alcohol sterilization etc. In the recognition degree on infection, the response that using method of ultrasonographic probes is insanitary were in 78.6%(33 hospitals), and 54.8%(23 hospitals) responded that bacteria can be infected through the probes. In the results of germiculture, bacteria and fungi were detected too number of to count, but escherichia coli was not detected. In conclusion, The gel stained on probe after ultrasonography must be cleared completely, and it is necessary that change of reco후ition on sanitary management. 초음파 검사 후 완전히 제거되지 않고 남아있는 Gel은 미생물이 증식하기에 좋은 조건을 가지고 있다. 따라서 본 연구자들은 초음파 탐촉자의 위생관리 실태와 위생과 감염에 대한 인식의 정도를 알아보기 위해서 2003년 12월부터 서울지역에 있는 42개의 병원을 대상으로 전화설문을 시행하였다. 또한 세 대의 초음파 장치 탐촉자에서 시료를 채취하여 미생물을 배양 증식한 후 탐촉자의 오염된 정도를 알아보았다. 21개 병원만이 탐촉자의 위생관리를 실시하였고, 소독 방법은 주로 알콜솜을 많이 이용하였다. 위생관리 주기는 14개 병원만이 매일 하는 것으로 조사되었다. 탐촉자에 묻은 Gel제거 방법은 면수건을 많이 이용 하였다. 감염의 위험에 노출되어 있는 환자의 초음파 검사 시 감염예방관리를 하고 있는 병원은 32개였고 환자 검사 시 탐촉자를 비닐커버나 외과용 장갑, 알콜 소독 후 사용하고 있는 것으로 조사되었다. 감염 인식도 조사에서 초음파 탐촉자 사용법이 비위생적이라는 생각이 78.6%(33개)였고, 탐촉자를 통한 세균이 감염여부에 대해 54,8%(23개)가 감염될 수 있을 것으로 생각하고 있었다. 탐촉자에서 채취한 시료를 배양 한 결과 세균과 진균이 셀 수 없이 너무 많이 오염되어 있었고 대장균은 검출되지 않았다. 결론적으로 초음파 검사 후에는 탐촉자에 묻어있는 Gel에서 세균이 증식하여 감염이 될 수 있으므로 완전히 제거하여 환자간 교차 감염의 위험요인을 줄이는 것과 감염관리에 대한 의식전환이 필요하다고 생각된다.
최미화(Mi-Hwa Choi),최경미(Kyeong-Mi Choi),지소영(So-Young Ji),이윤선(Youn-Sun Lee),조주현(Ju-Hyun Cho),이용문(Yong-Moon Lee),윤여표(Yeo-Pyo Yun),유환수(Hwan-Soo Yoo) 대한약학회 2010 약학회지 Vol.54 No.5
Glycosphingolipids are structural components of mammalian cell membranes and are involved in essential cellular physiology such as cell-cell interaction, recognition, transmembrane signaling, proliferation and cell death. In this study, the simple quantitative method of monoglycoceramides-containing glucosylceramide and galactosylceramide was developed. The glycosylceramides extracted from culture cells and rat plasma were resolved by TLC, deacylated by SCDase and analyzed by HPLC-fluorescence detector at an excitation wavelength of 340 nm and an emission wavelength of 455 nm. Limit of detection was approximately 0.1 pmol and limit of quantification was about 1 pmol for both monoglycoceramide standards. The recoveries of standard glucosylceramides from intra- and inter-day assays were 113.8 and 88.8% and those of galactosylceramides were 110.7 and 123.9%, respectively. The monoglycoceramide contents of SW-620 cells and rat plasma were 141.5±5 pmol/1×106 cells and 3.9±0.3 μM, respectively. The present analytical method provides a reproducible quantification and total content of monoglycoceramide which may be as a potential biomarker for lipid imbalance-related human diseases.