Detection of Salmonella typhi by Loop-mediated Isothermal Amplification Assay

조윤경,Jo, Yoon-Kyung,Lee, Chang-Yeoul 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.2

Salmonella typhi is frequent causes of foodborne illness and its detection is important for monitoring disease progression. In this study, by using general PCR and novel LAMP (Loop Mediated Isothermal Amplification) assay, we evaluated the usefulness of LAMP assay for detection of Salmonella typhi. In this LAMP assay, forward inner primer (FIP) and back inner primer (BIP) was specially designed for recognizing target invA gene. Target DNA was amplified and visualized as ladder-like pattern of bands on agarose gel within 60 min under isothermal conditions at $65^{\circ}C$. When the sensitivity and reproducibility of LAMP were compared to general PCR, there was no difference of reproducibility but sensitivity of LAMP assay was more efficient than PCR (the detection limit of LAMP assay was 30 fg, while the PCR assay was 3 pg). These results indicate that the LAMP assay is a potential and valuable means for detection of Salmonella typhi, especially for its rapidity, simplicity and low cost.

한국 임상병리사들의 은퇴 기대 조사

조윤경 ( Yoon-kyung Jo ),이덕혜 ( Duck-hye Lee ),성현호 ( Hyun-ho Sung ) 대한임상검사과학회 2017 대한임상검사과학회지(KJCLS) Vol.49 No.2

본 연구는 서울경기지역의 의료기관에 근무하는 임상병리사 158명을 대상으로 은퇴기대에 영향을 미치는 요인을 찾고자 자기기입식 설문조사를 실시하였다. 학력수준과 경제적 준비는 음의 상관성을 보였으나 성별, 월 소득 수준은 은퇴기대수준에 미치지 않는 요인이었다. 좌절감 수준에서는 연령과 근로시간면에서 유의한 증가를 보였으며, 교육수준과 결혼 상태는 은퇴준비에 미치는 요인이나 은퇴 기대에는 연관성이 없음을 알 수 있었다. 따라서, 연령, 교육수준, 가족, 은퇴기대 조건이 상호 연관성을 미치는 요인임을 알 수 있었다. 결론적으로 본 연구는 의료현장의 임상병리사들이 향후 은퇴기대 및 은퇴준비를 위해 영향인자를 발굴하는 기초정보를 제공하였고 향후 은퇴를 위한 교육, 심리적 수준, 직업특성을 고려하는 연구가 추가되어야 할 것으로 사료된다. This study investigated the influence factors of retirement expectations among medical technologists at medical centers in Seoul and Gyeonggi area, Korea. Subjects were 158 medical technologists. Using a sampling survey method, data was analyzed between July 2015 and December 2016. There was a negative correlation between educational level and economic preparation, but gender and monthly income did not reach retirement expectation level. Conversely, we found no change in the trends with respect to gender, monthly income; nonetheless, we observed that frustration gradually increased with age and working duration. In conclusion, our findings show that there are four factors that significantly influence retirement expectation and preparation: age, level of education, working duration, and family condition. This study contributes to understanding retirement expectations and can be a reference for retirement preparation in medical technologists.

뇌전증 EEG결과와 CBC결과의 관계연구

조윤경 ( Yoon Kyung Jo ),성현호 ( Hyun Ho Sung ),채경민 ( Kyoung Min Chae ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Epilepsy is a disorder that causes recurring seizures, and the most objective and useful test for detecting epilepsy is the electroencephalogram (EEG). The subjects of this study are 244 patients who received an EEG after being diagnosed with epilepsy at Seoul National University Hospital in 2014, and who have agreed to the purpose of the study. Based on the EEG results, subjects were divided into normal and abnormal groups with 122 subjects in each group, regardless of their gender and age, to investigate the correlation of EEG and complete blood cell count (CBC) test results. The four significant categories that displayed significant correlation between EEG results and CBC hematological measurements in this study were the white blood cell (WBC), red blood cell (RBC), neutrophil, and lymphocyte tests. The WBC (p<0.05) and neutrophil (p<0.01) showed a positive correlation with EEG results, while RBC (p<0.05) and lymphocyte (p<0.01) showed a negative correlation. One of the limitations of this study is that it is lacking the blood test result analysis according to the types of anti-epilepsy medicine. However, the analysis of EEG results by the same disease has significant meaning. Therefore, further studies are needed to statistically analyze more data in the future.

대학생의 체성분분석과 혈구산정검사의 관계연구

조윤경 ( Yoon-kyung Jo ),윤준 ( Joon Yoon ),조영국 ( Young-kuk Cho ),성현호 ( Hyun-ho Sung ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.3

국가의 공중 보건 정책을 개선하고 사회 경제적 발전을 결정하기 위하여 미래에 성인이 될 학생의 경향 연구는 매우 중요한 일이다. 본 논문은 남녀 대학생의 체중과 체질량지수 그리고 신체조성과 CBC 결과와 관계를 분석하는데 목적이 있다. 연구 결과, 신체조성 중에서 신장과 체중, 체질량지수 그리고 골격근 양, 기초대사량과 적혈구 수와 혈색소 그리고 적혈구용적률 등에서 양에 상관관계를 나타내었고 모두 통계적인 유의성이 있었다(p<0.01). 특히, 혈소판은 체지방량과 체지방률 그리고 허리와 엉덩이 비율에서 양의 상관관계로 나타났다(p<0.01). 이러한 연구결과는 대학생들의 건강관리에 필요한 기초자료로 의미가 있다. 따라서 대학생의 건강관리와 관련된 다량의 연구는 전략적으로 반드시 필요하다. 따라서 대학생들의 건강관리는 국가적으로 매우 중요한 부분을 차지할 수있으며, 지속적인 관리체계 구축과 그에 따른 제도적 마련이 필요하다고 사료된다. The tendency of students to become adults in the future to improve the country``s public health policy and to determine the socio-economic development of the research is very important. The objectives of this study were to examine the changes in body weight, body mass index (BMI), body composition, and complete blood cell count in a group of male and female university students. Among the body compositions, height, weight, BMI, muscle volume, basal metabolic rate, red blood cells, hemoglobin, and hematocrit showed a correlation between the amount shown both had statistical significance (p<0.01). In particular, platelets showed a rather different result from fat and body fat percentage, and were positively correlated with waist-hip ratio points (p<0.01). This study may be useful as it provides the basic data necessary for students of healthcare. Therefore, developing a sustainable management system of healthcare on a national level for university students is very important.

Ultrastructural Antigenic Localization in the Tissues of Echinostoma hortense Observed by Immunogold Labeling Method

Yoon-Kyung Jo(조윤경),Yong-Suk Ryang(양용석),Kyu-Je Lee(이규재),Insik Kim(김인식),Jang-Keun Ryu(류장근),Mi-Sook Song(송미숙) 대한의생명과학회 1999 Biomedical Science Letters Vol.5 No.2

호르텐스극구흡충을 흰쥐에게 경구 감염시키고 4주 후에 성충을 획득하여 고정, 포매 및 절편하여 조직항원 분포 부위를 관찰하였다. 호르텐스극구흡충의 항체는 호르텐스극구흡충 피낭유충을 흰쥐에게 경구 감염시키고 4주 후에 채혈한 혈청과 호르텐스극구흡충 조항원을 면역증강제와 함께 흰쥐에게 근육 주사하여 채혈한 혈청을 이용하였으며, 이 혈청을 반응시켜서 호르텐스극구흡충 조직 내의 항원성 분포를 규명하였다. 한편, 면역황금표식법에 의한 미세조직의 항원성 반응 여부는 조직에 표지된 황금입자로 판정하였다. 그 결과 충체 조직에서 황금입자와 강하게 반응하는 미세조직은 표피층의 표지합포체, 난황세포의 구성물질, 유연조직의 분비과립 그리고 배설낭의 구성물질 등에서 반응이 강하게 나타났다. 이상의 결과를 볼 때 호르텐스극구흡충의 항원은 생식세포와 소화분비 및 소화관계통에서 분비 유래되는 것으로 분석되었다. This study involved applying rat sera (control, infected and immunized) to adult worm tissue in order to measure the antigenic response. A serum was obtained from rats infected with E. hortense metacercaria for 4 weeks. Another immunized serum was taken from rats given a muscle injection with crude adult worm antigen. The detection of antigenic response in E. hortense tissue was made by immunogold labeling method and measured through gold particles impregnated in the tissue. The antigenic sites, those with the highest density of gold particles, were the tegmental syncytium, vitelline cells, seminal receptacle and cecum.

보문 : 효율적인 스플릿(Split) 그레이딩 방식의 어패럴 CAD 시스템 사용방법에 관한 연구 : 국내 사용업체들의 실태조사를 통한

조윤경 ( Yoon Kyung Cho ),조진숙 ( Jin Sook Jo ) 韓國衣類學會 1996 한국의류학회지 Vol.20 No.6

혈색소 농도에 따른 대학생의 골밀도 분석

윤준 ( Joon Yoon ),김대중 ( Dai-joong Kim ),성현호 ( Hyun-ho Sung ),조윤경 ( Yoon-kyung Jo ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.4

This study was performed to evaluate the effect of hemoglobin (Hb) on bone mineral density (BMD) in university students by performing a quantitative analysis. The subjects included healthy university students aged 20 to 30 years. Although osteoporosis has traditionally been considered as a disease of aging women, it is becoming an increasingly concerning male health problem. Diagnosis of osteoporosis is calculated with a quantitative assessment of BMD. Laboratory blood and urine tests are mainly used with low BMD or fragility fractures to identify any possible causes of bone metabolism disorders. In this study, there was no difference in BMD according to gender. The average red blood cell (RBC), Hb, and Hematocrit (HCT) were significantly higher in males (p<0.01). The correlation between lumbar spine, skeletal muscle mass (SMM), and basal metabolic rate (BMR) was statistically significant (p<0.01). Hb showed a 51.7% statistical influence on BMD by multiple regression analysis. These findings are useful to understand the relationship between BMD and Hb; lower Hb level is associated with lower BMD. The Hb level was the strongest predictor of abnormal BMD. In conclusion, this study showed that a low Hb value was significantly correlated with low bone mass, suggesting that a low Hb value is a risk factor for changes in bone turnover that leads to a decrease bone density.

SDS-PAGE and Immunoblot Patterns of Echinostoma hortense in Experimentally Infected Rats

Yong-Suk Ryang(양용석),Yoon-Kyung Jo(조윤경),Ji-Sook Lee(이지숙) 대한의생명과학회 1998 Biomedical Science Letters Vol.4 No.1

저자들은 호르텐스극구흡충의 항원 단백질과 일부 특이 항원을 알아보기 위해 다음과 같이 실험을 실시하였다. 호르텐스극구흡충 충체를 추출하여 제조한 조항원을 재료로 SDS-PAGE를 실시하여 항원 단백질을 분석하였다. 그리고 흰쥐 (rat)에게 호르텐스극구흡충 피낭유충을 감염시켜 얻은 혈청 항체와, 호르텐스극구흡충 조항원과 면역증강보조제를 함께 투여하여 얻은 혈청 항체를 재료로 하여 EITB를 실시하여, 일부 특이 항원을 규명하였으며 성적은 다음과 같다. 즉, 조항원으로 SDS-PAGE를 실시한 결과는 200.2-8.2kDa까지 46개의 분획이 관찰되었고, 이중에서 특히 강하게 관찰된 단백분획은 200.2, 107.9, 86.8, 75, 69.8, 46.8, 43.5, 34.5, 20.9, 13.6, 12.6, 11.7, 8.2 kDa이었다. 그리고 EITB를 실시하여 분석한 특이 IgG 항체는 198-13.7 kDa까지 17개의 분획을 보였고, 특히 면역염색이 강하게 나타난 분획은 198, 123.4, 100.8, 91.1, 88.1, 62.8, 34.2, 32, 29.9, 18, 15.7, 13.7 kDa이었다. The authors characterized the antigen proteins and some specific antibodies from Echinostoma hortense. Crude antigen extracted from E. hortense worm was analyzed by SDS-PAGE of the crude antigen showed 46 profiles betweeen 200.2 - 8.2kDa, among which 200.2, 107.9, 86.8, 75, 69.8, 46.8, 43.5, 34.5, 20.9, 13.6, 12.6, 11.7, and 8.2kDa, protein profiles were strong. EITB resolved the specific IgG antibody into 17 profiles between 193 - 13.7kDa, among which 198, 123.4, 100.8, 91.1, 88.1, 62.8, 34.2, 32, 29.9, 18, 15.7, 13.7kDa profiles showed strong immunostain.

Photodynamic Therapy for Methicillin-resistant Staphylococcus aureus Using Various Photosensitizer

Pil Seung Kwon,Yoon kyung Jo(조윤경) 대한의생명과학회 2009 Biomedical Science Letters Vol.15 No.3

The aim of this study was to evaluate the photodynamic effect of various photosensitizing agents against methicillinresistant Staphylococcus aureus (MRSA). MRSA was exposed to light from a 632 ㎚ diode laser (15 J/㎠) in the presence of various photosensitizer, such as photofrin, photogem, radachlorine and ALA. In vivo study was performed using ICR mice. Twenty eight mice had a standard wound (100 ㎟) created on the dorsum, and MRSA was inoculated into the wound region. The four groups were classified as follows: (1) the untreated control group (bacteria alone), (2) the bacteria plus light group (15 J/㎠), (3) the bacteria plus photofrin group (kept in the dark), and (4) the photodynamic therapy (PDT) group (bacteria, photofrin, and light). After photofrin (dose 1 mg/kg) injection, the experiemental group was irradiated with 632 ㎚ diode laser (15 J/㎠) for 30 minutes after In vitro results of PDT showed the complete killing of MRSA at the photofrin, radachlorine, and photogem However, ALA-PDT was ineffective on MRSA viability. In vivo results showed that photofrin has therapeutic effect on the wound infection. These results demonstrate that selective lethal photosensitization of MRSA can be achieved using phofrin, photogem and radachlorin. Thus, PDT can inactivate MRSA survival.

일부 대학생들의 치주건강에 대한 주관적 인식과 혈구수치와의 상관관계

이선미 ( Sun Mi Lee ),임미희 ( Mi Hee Lim ),조윤경 ( Yoon Kyung Jo ) 한국치위생학회(구 한국치위생교육학회) 2013 한국치위생학회지 Vol.13 No.3

Objectives : The purpose of this study was to examine the periodontal health indexes of some college students and awareness of periodontal health by conducting a survey and complete blood count(CBC) to evaluate periodontal health status. Methods : The study subjects were 133 college students. After receiving informed consent, the health-related majoring students voluntarily participated in this study from May 1 to 30, 2012. Results : 1. In order to assess periodontal health indexes, total scores of all the 15 items were calculated and mean was 3.06 of 5 points. Mean of periodontal health was 3.48. 2. High hemoglobin and high hematocrit revealed high periodontal health indexes and high platelet resulted in low peridontal health indexes. 3. Red blood cell, hemoglobin, and hematocrit of the male, older, smoking, and high periodontal index students showed higher range of score in the meanwhile white blood cell and platelet was low range. The range of female students were not statistically significant. Conclusions : Periodontal health education program is very important to periodontal care and can motivate the oral health behavior change.