밀 F₁ 약배양에 의한 식물체 분화 및 분화식물체의 배수성

권순종(Soon Jong Kweon),박동수(Dong Soo Park),고종민(Jong Min Ko),현종내(Jong Nae Hyun),서득룡(Duck Yong Suh),서형수(Hyung Soo Suh),남중현(Jung Hyun Nam),홍병희(Byung Hee Hong),오윤진(Yun Jin Oh) 한국육종학회 1997 한국육종학회지 Vol.29 No.2

Five combinations of early maturity wheat F₁ were conducted to anther culture and 345 green plants were obtained. Callus induction rate was 4.1%, plant regeneration rate 46%, green plant rate 88%, respectively. Liquid medium was effective to induce callus of wheat anther. Chromosome analysis of 102 plants showed 5 diploids, 1 aneuploid and 96 haploids. For breeding with anther culture in wheat, chromosome doubling process will be necessary.

해충저항성 Bacillus thuringiensis (Bt) 벼의 환경위해성 평가: 해충저항성 Bt벼가 물벼룩(Daphnia magna)에 미치는 영향

이기종 ( Ki Jong Lee ),김효진 ( Hyo Jin Kim ),이장용 ( Jang Yong Lee ),류태훈 ( Tae Hun Ryu ),박종석 ( Jong Sug Park ),박범석 ( Beom Seok Park ),권순종 ( Soon Jong Kweon ),손수인 ( Soo In Sohn ),서석철 ( Seok Cheol Suh ),오성덕 ( 한국응용생명화학회(구 한국농화학회) 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.4

해충저항성 Bacillus thuringiensis (Bt) 벼와 낙동벼의 비표적생물체인 물벼룩(Daphniamagna)에 대한 급성독성시험을 실시한 결과, 해충저항성 Bt벼의 48시간-EC50은 4,429.13 mg/L (95% 신뢰한계는 3908.130~5020.363 mg/L), 무영향 농도(NOEC)는 1,800 mg/L이었고, 낙동벼는 48시간-EC50은 2,889.56 mg/L (95% 신뢰한계는 1,073.407~6,854.321 mg/L), 무영향농도는 1,000 mg/L이었다. 처리기간 중 해충저항성 Bt벼와 낙동벼간의 급성독성에 영향을 미칠 수 있는 요인은 발생하지 않았다. Insect-resistant transgenic rice was developed by inserting the mCry1Ac1 a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For biosafety assessment, we studied the effects on survival of cantor Daphnia magna, a commonly used as a model organism in ecotoxicological studies. D. magna fed on Bt rice and its near non-genetically modified (GM) counterparts (Nakdong) grown in the same environment (100% ground rice suspension). The Bt rice was comfirmed to have the insertion of T-DNA and protein expression by the polymerase chain reaction and ELISA analysis. Feeding study showed similar cumulative immobility and abnormal response of D. magna between Bt rice and non-GM counterparts. 48 h-EC50 values of Bt rice and non-GM rice showed 4,429 and 2,889 mg/L respectively. The rice no observed effect concentration (NOEC) values for D. magna was suggested 1,000 mg/L. We conclude that the tested Bt-rice and Nakdong similar cumulative immobility for D. magna the widely used model organism. We found out that there is strong possibility that the growth of Bt rice didn`t affect to non-target insects.

연구보문 : 환경생물 ; 형질전환 콩 재배가 근권 토양 미생물상에 미치는 영향

이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),이장용 ( Jang Yong Lee ),이부영 ( Bu Young Yi ),오성덕 ( Sung Dug Oh ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈 ( Tae Hun Ryu ),김경환 ( Kyung Hwan Kim ),박종석 ( Jo 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4

BACKGROUND: Soybean [Glycine max (L.) Merrill] is legume and an important oil crop worldwide. This study was conducted to evaluate the possible impact of transgenic soybean cultivation on the soil microbial community. METHODS AND RESULTS: Microorganisms were isolated from the rhizosphere soils. Microbial community was identified based on the culture-dependent and molecular biology methods. The total numbers of bacteria, fungi, and actinomycete in the rhizosphere soils cultivated with transgenic and non-transgenic soybeans were similar to each other, and there was no significant difference between transgenic and non-transgenic soybeans. Dominant bacterial phyla in the rhizosphere soils cultivated with transgenic or non-transgenic soybeans were Actinobacteria, Firmicutes, and Proteobacteria. The microbial communities in transgenic and non-transgenic soybean soils were characterized using the denaturing gradient gel electrophoresis (DGGE). The DGGE profiles showed the different patterns, but didn`t show significant difference to each other at 0.05 significance level. DNAs were isolated from soils cultivating transgenic or non-transgenic soybeans and analyzed for persistence of transgenes in the soil by using PCR. PCR analysis revealed that there were no amplified γ-tmt and bar gene in soil DNA. CONCLUSION(S): The results of this study suggested that microbial community of soybean field were not significantly affected by cultivation of the transgenic soybeans.

중·북부지역에서 국내육성 호밀품종의 채종방법

한옥규,송주희,구자환,김대욱,권영업,이유영,박장환,권순종,안종웅,Han, Ouk-Kyu,Song, Ju-Hee,Ku, Ja-Hwan,Kim, Dea-Wook,Kwon, Young-Up,Lee, Yu-Young,Park, Chang-Hwan,Kweon, Soon-Jong,Ahn, Jong-Woong 한국초지조사료학회 2018 한국초지조사료학회지 Vol.38 No.1

국내 호밀 종자의 자급률을 향상시키기 위해 중부지역에 적합한 종자 채종기술을 개발하고자 2013~2015년까지 3개년에 걸쳐 경기도 수원에서 시험을 실시하였다. 품종은 국내 육성종인 곡우호밀을 사용하였고, 시험구는 파종량 3수준(3, 5, 7 kg/10a), 질소시비량 3수준(3, 6, 9 kg/10a)의 분할구배치법 3반복으로 하였으며, 생육특성과 종실 생산성을 평가하였다. 호밀 종자의 파종량을 늘리면 $m^2$당 수수가 증가하지만, 유효경 비율 1수립수 임실률 리터중 및 천립중이 다소 감소하는 경향을 보였으나 수량 증가에는 영향이 크지 않았다. 질소시비량을 늘리면 $m^2$당 수수와 1수립수가 많아지고 임실률 등의 증가로 수량이 증가하였다. 그리고 호밀의 종실수량은 파종량과 질소시비량 간 교호작용을 보이지 않았다. 국내육성 호밀품종의 종자생산 시 생산성과 더불어 종자 및 비료절감, 환경보호 등을 고려할 때 10a당 적정 파종량은 5 kg, 질소시비량은 5~6 kg 이었다. This experiment was conducted at Suwon, Korea from 2013 to 2015. The objective of this study was to establish the optimum seeding rate, and to clarify the nitrogen fertilizer level for rye seed production in central and north area of Korea. We used Korean rye cultivar 'Gogu' for this test. We employed a split-plot design with three replications. The main plots were designed by three seeding levels (3, 5 and $7kg\;10a^{-1}$), but other sub-plots were randomly seeded. The plots were treated with three different nitrogen fertilizer levels (3, 6 and $9kg\;10a^{-1}$). The percentage of productive tiller, number of grain per spike, fertility rate, 1 liter weight, and 1000-grain weight decreased as seeding rate increased from $3kg\;10a^{-1}$ to $7kg\;10a^{-1}$, whereas the number of spike per $m^2$ increased. Therefore the grain yields of rye had less of an effect by increasing seeding rate. There was an increase in number of spike per $m^2$, number of grain per spike, and fertility rate as nitrogen fertilizer level increased from $3kg\;10a^{-1}$ to $9kg\;10a^{-1}$, but grain yields significantly not affected by the interaction of seeding rate ${\times}$ nitrogen fertilizer levels. However, the best seeding rate and nitrogen fertilizer level for rye seed production were 5 kg and $5{\sim}6kg\;10a^{-1}$, respectively, considering seed and fertilizer reduction and the prevention of pollution by excess fertilization.

형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석

친양(Yang Qin),권순종(Soon-Jong Kweon),정영수(Young-Soo Chung),하선화(Sun-Hwa Ha),신공식(Kong-Sik Shin),임명호(Myung-Ho Lim),권택(Taek-Ryoun Kwon),조현석(Hyun-Suk Cho),박순기(Soon Ki Park),우희종(Hee-Jong Woo) 한국육종학회 2015 한국육종학회지 Vol.47 No.2

The β-carotene biofortified transgenic soybean was developed recently through Agrobacterium-mediated transformation using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan). GM crops prior to use as food or release into the environment required risk assessments to environment and human health in Korea. Generally, transgenic plants containing a copy of T-DNA were used for stable expression of desirable trait gene in risk assessments. Also, information about integration site of T-DNA can be used to test the hypothesis that the inserted DNA does not trigger production of unintended transgenic proteins, or disrupt plant genes, which may cause the transgenic crop to be harmful. As these reasons, we selected four transgenic soybean lines expressing carotenoid biosynthesis genes with a copy of T-DNA by using Southern blot analysis, and analyzed the integration sites of their T-DNA by using flanking sequence analysis. The results showed that, T-DNA of three transgenic soybean lines (7-1-1-1, 9-1-2, 10-10-1) was inserted within intergenic region of the soybean chromosome, while T-DNA of a transgenic soybean line (10-19-1) located exon region of chromosome 13. This data of integration site and flanking sequences is useful for the biosafety assessment and for the identification of the β-carotene biofortified transgenic soybean.

친환경 농법을 이용한 볼리비아 차코지역 옥수수 생산성 향상 연구

박근희 ( Geun Hee Park ),권순종 ( Soon Jong Kweon ),후안헤레즈라마스 ( Jerez Lamas Juan ) 한국국제협력단 ODA도서관 2023 국제개발협력 Vol.2023 No.1

Corn is an important food security crop in Bolivia. Consumption is approximately divided into 77% animal feed and 17% human consumption. However, Bolivian corn production is the lowest among its neighboring countries due to the use of uncertified varieties, lack of fertilizer use, limited agricultural technology, and climate change. Korea Partnership for Innovation of Agriculture Bolivia Center and Bolivian Institute of Agricultural and Forestry Innovation planted synthetic INIAF Guaraní and hybrid INIAF H1 using green manure and Biol (eco-friendly fertilizer) in the Chaco region. Three villages used green manure for crops. Later, INIAF Guaraní and INIAF H1 were sown using Biol. The production and generated income were calculated based on the market price of 1,300 Bs per ton. Traditional farming methods for INIAF Guaraní and INIAF H1 yield 2.4 tons/ha and 5 tons/ha, respectively. By contrast, using green manure and Biol yielded 5.2 tons/ha for INIAF Guaraní (1.8 tons/ha increase) and 6.5 tons/ha for INIAF H1 (1.5 tons/ha increase). Net income from traditional farming methods was 980 Bs, whereas using ecofriendly farming methods to cultivate INIAF Guaraní and INIAF H1 resulted in net incomes of 2,620 Bs and 3,910 Bs, respectively. The project highlights Bolivia’s need to disseminate techniques that use certified varieties and eco-friendly farming methods through demonstrations for farmers and village pilot programs.

무선발표지 형질전환 식물체 제조기술

우희종,신공식,이기종,권순종,조용구,서석철,Woo, Hee-Jong,Shin, Kong-Sik,Lee, Ki-Jong,Kweon, Soon-Jong,Cho, Yong-Gu,Suh, Seok-Cheol 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2

Selectable marker gene systems are vital for the development of transgenic plants, but the presence of selectable marker genes encoding antibiotic or herbicide resistance in genetically modified plants poses a number of problems. A lot of research results and various techniques have been developed to produce marker-free GM plants. The aim of this review is to describe the principal methods used for eliminating selectable marker genes to generate marker-free GM plants, concentrating on the three significant methods(co-transformation, site-specific recombinase-mediated excision, non-selected transformation) in several marker-free techniques.

연구보문 : 알파토코페놀 고 함유 유전자변형 들깨에 대한 검정법 개발

신공식 ( Kong Sik Shin ),우희종 ( Hee Jong Woo ),이기종 ( Ki Jong Lee ),김경환 ( Kyung Hwan Kim ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Cheol Suh ) 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.4

알파토코페놀을 증가시키기 위해서 γ-TMT(γ-tocopherol methyltransferase)유전자로 형질전환 된 유전자변형 들깨가 개발되었고, 도입유전자의 검출법 개발을 위하여 정성 및 정량 PCR 분석을 수행하였다. 도입유전자 및 들깨 내재유전자를 바탕으로 하여 몇 개의 검출 primer쌍을 제조하였으며, 정성 PCR 방법으로 primer의 특이성을 조사하였다. 들깨 내재 유전자, KAS-I의 증폭을 위해서 KASI02-1/2 primer를 사용하여 195 bp 크기의 PCR 증폭산물을 얻었으며, 도입유전자에 대하여 구조 특이적 primer, TMTocs-1/2 및 VicTM-1/2를 이용하여 유전자변형들깨를 포함한 6개 작물과 국내 개발된 3개 유전자변형 작물에 대해 PCR을 수행한 결과에서 각각 191 bp 및 109 bp의 PCR 산물이 유전자변형 들깨에서만 특이적으로 증폭되는 것을 확인하였다. 정량 검출을 위해서 plasmid, pKAViTM을 제조하였고, 이를 표준시료로 하여, 0.3, 1 및 1.5%로 조제된 유전자변형 들깨를 real-time PCR로 분석함으로써 유의성 있는 결과를 얻을 수 있었으며, 이의 방법이 유전자변형 들깨를 정량적으로 검정하는데 적용될 수 있음을 확인하였다. Qualitative and quantitative PCR methods were performed to examine the detection of γ-TMT(γ-tocopherol methyltransferase) inserted into genetically modified(GM) perilla(Perilla frutescens)developed in Korea. Several primer pairs were prepared on introduced genes and an endogenous reference gene in perilla. Specificity of primers first was tested by the means of qualitative PCR analysis. Primer pair KASI02-1/2 was used to amplify the endogenous gene, KAS-I, and gave rise to an amplicon 195 bp. PCR amplification using the construct-specific primer pairs, TMTocs-1/2 and VicTM-1/2 also was performed for GM perilla. TMTocs-1/2 and VicTM-1/2 primers gave rise to an amplicon 191 bp and 109 bp, respectively. In contrast, no amplified product was observed when DNA samples from 6 different plants and 3 GM crops were used as templates. For quantitative detection, test samples containing 0.3, 1, and 1.5% genetically modified perilla were measured by real-time PCR using the plasmid DNA, pKAViTM as standard material. This result showed real-time PCR method was applicable to detect GM perilla quantitatively.

종자 생산량이 많은 호밀 신품종 "씨드그린"

한옥규,황종진,박형호,김대욱,오영진,박태일,구자환,권영업,권순종,박광근,Han, Ouk-Kyu,Hwang, Jong-Jin,Park, Hyung-Ho,Kim, Dea-Wook,Oh, Young-Jin,Park, Tae-Il,Ku, Ja-Hwan,Kwon, Young-Up,Kweon, Soon-Jong,Park, Kwang-Geun 한국초지조사료학회 2015 한국초지조사료학회지 Vol.35 No.2

국립식량과학원에서 국내 환경에 적합한 조사료용 호밀 품종을 개발하기 위하여 1995년에 '조춘호밀' 등 10개 품종을 방임수분으로 혼합 교배하여 그 중에서 형질이 우수한 'SR95POP-S1-140-9-1-3-7-5-3' 계통을 선발하였다. 선발 계통에 대해 생산력을 검정한 결과 특성이 우수하여 '호밀 50호'로 계통명을 부여하고 5개 지역에서 3개년간 지역적응성을 검정하였으며, 조사료 수량뿐만 아니라 종실 수량이 높은 것으로 나타나 2013년 직무육성신품종 선정위원회에서 신품종으로 선정됨과 함께 '씨드그린'으로 명명되었다. '씨드그린'은 초형이 직립이고, 잎색은 농록색이며, 길이는 길고, 폭은 넓은 편이다. 줄기는 황백색으로 중간 정도의 굵기이며, 종실은 갈색이고, 중간 크기이다. '씨드그린'의 출수기는 4월 22일로 표준품종인 '곡우' 보다 3일, 성숙기는 6월 16일로 2일 빠르다. 초장은 141 cm로 '곡우' 보다 3 cm 길다. 한해는 평균 2로 표준품종인 '곡우'의 1과 대등한 수준이며, 도복은 '곡우' 보다 약간 강하였으며, 습해와 병해는 발생하지 않았다. '씨드그린'의 생초수량은 ha 당 39.8톤(곡우 40.8톤), 건물수량은 평균 8.3톤(곡우 8.4톤)으로 '곡우' 보다 약간 적었으나, 종실수량은 4톤으로 '곡우'보다 16% 증수하였다. '씨드그린'의 조단백질 함량은 10.5%로서 '곡우' 보다 0.8% 높았으며, ADF와 NDF 함량(%)은 각각 38.8%와 66.4%로 '곡우' 보다 낮았으나, TDN 함량(%)은 58.3%로 '곡우' 보다 1.3% 높았으며, TDN 수량 또한 4.81 톤/ha로 '곡우' 보다 약간 높았다. "Seedgreen" (Secale cereal L.), a new rye cultivar was developed by National Institute of Crop Science (NICS), RDA in 2013. It was developed from an open pollination from within 10 rye varieties or lines including "Chochun" in 1995. The line "SR95POP-S1-140-9-1-3-7-5-3" was selected for its excellent agronomic appearance, and was placed in yield trials for three years from 2008 to 2010. The new cultivar was designated "Homil50" and was placed in regional yield trials at the five locations around Korea from 2011 to 2013, during which time the name "Seedgreen" was given. This cultivar is an erect plant type and of a long size, with a dark-green leaf color, a yellowish-white colored, medium-diameter culm, and a brown-colored, medium-size grain. The heading and maturation dates of Seedgreen were April 22 and June 16, which were 3 days and 2 days earlier than that of "Gogu", respectively. Seedgreen also showed better winter hardiness and a greater resistance to lodging and wet injury compared to those of the check cultivar. Over three years, the average dry matter yield of Seedgreen was 8.3 ton $ha^{-1}$ (fresh yield = 39.8 ton $ha^{-1}$), which was harvested in late April and was lower than that of the check cultivar Gogu. The seed productivity of Seedgreen was approximately 4 ton $ha^{-1}$, which was 16 % more than that of the check. Seedgreen was higher to than Gogu in term of protein content (10.5% and 9.7%, respectively), total digestible nutrients (TDN) (58.3% and 57%, respectively), and TDN yield $ha^{-1}$ (4.81 ton and 4.77 ton, respectively). This cultivar is recommended as a fall sowing crop in areas where the average daily minimum-mean temperatures are higher than $-12^{\circ}C$ in January, and as a winter crop for whole-crop forage before the planting of rice or green manure around Korea.

연구논문 : 자연과학 ; 해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향

오성덕 ( Sung Dug Oh ),이기종 ( Ki Jong Lee ),손수인 ( Soo In Sohn ),권용정 ( Yong Jung Kwon ),김진서 ( Jin Seo Kim ),이장용 ( Jang Yong Lee ),박범석 ( Beom Seok Park ),권순종 ( Soon Jong Kweon ),서석철 ( Seok Choel Suh ),류태훈· 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2

해충저항성 Bacillus thuringiensis (Bt) 벼의 비표적곤충인 벼물바구미(Lissorhoptrus oryzophilus)에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 72시간-LC50은 0.245 ml/L(95% 신뢰한계는 0.195~0.307 ml/L)이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간- LC50은 0.257 ml/L(95% 신뢰한계는 0.199~0.331 ml/L)이었다. 72시간-LC50은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72시간-LC50이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종의 LC50값에 유의성이 없는 것으로 판단된다. Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis, As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-LC50 values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively, As the 72h-LC50 vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-LC50 values were not significant, Thus, we found out there was a strong possibility that Bt rice didn`t affect the insecticide susceptibilities of nontarget insects.