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RISS 인기검색어
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- 저자 : So-jeo Ahn(안소저) (검색결과 1 건)
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Development of Rapid Detection Technique for Canine Distemper Virus
김두,안소저,권혁무,Kim, Doo,Ahn, So-Jeo,Kwon, Hyuk-Moo The Korean Society of Veterinary Clinics 2000 한국임상수의학회지 Vol.17 No.1
Recently cases of canine distemper have occurred in Korea despite vaccination was carried out nationwidely. This study was performed to establish rapid diagnosis of canine distemper by RT-PCR, nested PCR, and serological test. A total of 30 dogs, which were suspected canine distemper clinically, was examined. RT-PCR and nested PCR were specific for the amplification of CDV H gene and sensitive to detect 7 TCID50 of Onderstepoort strain. By RT-PCR, H gene was detected in 6(20%) of 30 peripheral bloods from dogs. And H gene was detected in 10(33.3%) of 30 samples by nested PCR. H gene was detected from 1 brain of 6 years-old Beagle dog and 1 lung of 2 months-old Shihtzu dog, in which peripheral blood H gene was not detected. Serum neutralizing antibody titer against Onderstepoort strain ranged from 4 to 1,024 in 30 patients. No correlation was observed between the results of nested PCR and titiers of neutralizing antibody.
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