자연체험에 기초한 원예활동이 유아의 정서 지능 및 자연친화적 태도에 미치는 영향

류승희(Seung-Hee Ryu),박유영(Yu-Yeong Park),이승우(Seung-Woo Lee) 중앙대학교 한국교육문제연구소 2013 한국교육문제연구 Vol.31 No.3

본 연구는 가까운 주변의 자연을 지속적으로 경험할 수 있는 자연체험에 기초한 원예 활동이 유아의 정서 지능 및 자연 친화적 태도에 어떠한 영향을 미치는지를 비교, 분석하여 그 효과를 검증 해 봄으로써 실제 유아교육 현장에서 유아의 정서지능과 자연친화적 태도 발달을 위한 교육적 방향과 교수학습방법을 모색하고자 하는데 그 목적이 있다. 만 5세 유아 30명을 대상으로 실험집단에는 자연체험에 기초한 원예활동을 실시하고 비교집단에는 산책활동을 실시한 뒤 정서지능검사(이병래, 1997)와 자연친화적 태도 검사(소경희, 2007)를 실시하였다. 연구결과 자연체험에 기초한 원예활동이 유아의 정서지능 전체 점수와 하위영역인 자기정서인식, 자기정서조절, 타인정서인식, 타인정서조절에서 효과적인 것으로 나타났다. 또한 자연체험에 기초한 원예활동이 유아의 자연친화적 태도 전체 점수와 하위영역인 동물·식물에 대한 관심과 애호, 자연환경에 대한 선호, 자연보호에서 효과적인 것으로 나타났다. The purpose of this study was to discover teaching-learning methods for young children’s development of emotional intelligence and nature-friendly attitudes. This objective was met by analyzing the emotional intelligence and nature-friendly attitudes of the group of children after creating continual gardening experiences in the fields near the daycare centers. The subjects were 30 five-year-old children. The experimental group participated in gardening activities promoting nature-experience, while the comparative group participated only in walking activities. The following sources were used as research tools: Lee, Bung-Lae’s emotional intelligence test(1997) and So, Kyeong-Hee’s a nature-friendly attitude test(2007). The results were as follows: Gardening activities promoting nature-experience effected not only the total points of children’s emotional intelligence, but also their recognition of self-emotion, control of self-emotion, recognition of others’ emotional state, and control of others’ emotions. Moreover, gardening activities promoting nature-experience effected the total points of children’s nature-friendly attitudes and their interest and affection for animals and plants, they had a preference for the natural environment, and protection of nature.

김치종류별 용매 획분의 자외선 B 조사에 의해 유도된 피부산화 및 홍반 생성 억제 효과

류복미(Bog-Mi Ryu),류승희(Seung-Hee Ryu),전영수(Young-Soo Jeon),이유순(Yu-Soon Lee),문갑순(Gap-Soon Moon) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.5

배추김치, 갓김치, 부추김치의 용매획분 추출물을 제조한 뒤 항산화관련 물질들의 함량을 측정한 결과 카로테노이드는 갓김치의 hexane층에서, 클로로필 함량은 부추김치의 hexane층에서 가장 높았고, 비타민 C 함량은 갓김치의 EtOAc 층과 BuOH층에서 가장 높게 나타났다. 총 페놀화합물은 부추김치의 CH₂Cl₂층과 EtOAc층, 배추김치 CH₂Cl₂층, 갓김치 EtOAc층과 CH₂Cl₂층에 많이 함유되어 있었다. DPPH법을 이용한 유리기 소거능을 조사한 결과 부추김치 CH₂Cl₂층과 EtOAc층, 배추김치 CH₂Cl₂층, 갓김치 EtOAc층에서 효과가 큰 것으로 나타났다. 김치종류별 용매획분을 hairless mouse의 피부균질액에 첨가하여 자외선 B를 조사한 후 지질과산화물 함량을 측정한 결과 부추김치의 CH₂Cl₂층과 EtOAc층, 배추김치, 갓김치의 EtOAc층에서 낮게 나타나 항산화성이 높은 획분에서 피부의 광산화 억제효과가 큰 것을 알 수 있었고 김치 용매획분을 크림에 첨가하여 hairless mouse의 등에 직접 도포한 후 자외선 B를 조사하였을 때 대조군에 비해 갓김치의 hexane층, 부추김치의 hexane층과 BuOH층을 제외한 모든 용매획분이 피부홍반의 생성을 억제하였고 특히 갓김치 EtOAc층, 배추김치 CH₂Cl₂층, 부추김치 물층에서 피부보호효과가 큰 것으로 나타났다. Kimchi is well known as a traditional Korean food containing various antioxidative compounds and it has been demonstrated that dietary kimchi inhibit the tissue oxidation and aging in many related studies. Ultraviolet B (UVB) radiation is the most potent among the many related factor to skin photo-aging. This experiment is designed to elucidate the inhibitory effect of solvent fractionation of various kimchi on UVB induced oxidation and erythema formation. Among solvent fractions, phenol content mostly existed in dichloromethane / ethylacetate fraction of buchu kimchi, dichloromethane fraction of Korean cabbage kimchi and ethylacetate / dichloromethane fraction of mustard leaf kimchi. Free radical scavenging activities were higher in dichlo-romethane and ethylacetate fraction from buchu kimchi, dichloromethane fraction from Korean cabbage kimchi, and ethylacetate fraction from mustard leaf kimchi. When the damage of photo-oxidation by UVB irradiation was tested in the presence of solvent fractions of kimchi, dichloromethane / ethylacetate fraction of buchu kimchi, ethylacetate fraction from Korean cabbage and from mustard leaf kimchi showed the higher protective activities than others. The erythema formations which were induced by UVB irradiation were decreased in solvent fractions of kimchi that have higher antioxidant activities. In conclusion, kimchi solvent fractions having antioxidant compounds mostly inhibited photo-oxidation and erythema formation by UV-irradiation, therefore kimchi consumption can retard skin aging due to the presence of antioxidative compounds.

김치종류별 식이가 Hairless Mouse 피부의 형태학적 변화에 미치는 영향

류복미(Bog-Mi Ryu),류승희(Seung-Hee Ryu),양영철(Young-Churl Yang),이유순(Yu-Soon Lee),전영수(Young-Soo Jeon),문갑순(Gap-Soon Moon) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.9

피부는 외부의 물리적 화학적 자극으로부터 신체를 보호하는 중요한 역할을 하며 노화에 따른 조직의 형태학적인 변화를 쉽게 관찰할 수 있는 기관이다. 동결건조한 배추김치, 갓김치, 부추김치를 식이에 10%씩 첨가하여 hairless mouse에 16주간 섭취시켜 피부조직에 미치는 영향을 살펴보았다. 노화가 진행됨에 따라 표피의 두께는 감소하며 본 실험에서 대조군에 비해 김치섭취군에서 유의적으로 두꺼운 것으로 나타났다. 피부 각질화에 관여하는 cytokeratin은 대조군에 비해 갓, 배추, 부추김치군의 순으로 낮았는데 전자현미경적 소견으로도 대조군에서 각질층은 중간원섬유들이 치밀하게 분포된 매우 두꺼운 각질층이 보인 반면 갓김치, 배추김치, 부추김치군은 얇은 각질층으로 배열되어 있었다. 상피기저막의 type Ⅳ 콜라겐은 갓김치섭취군에서 현저하게 증가되어 갓김치 성분들이 피부의 기저막의 노화를 억제한 것으로 사료된다. 콜라겐 함량은 노화에 따라 감소하며 대조군에서 콜라겐의 합성에 관여하는 fibroblast의 RER 발달은 뚜렷하지 않았으나 갓김치군, 배추김치군에서는 RER의 발달이 현저하여 새로운 콜라겐 생성이 활발히 이루어지는 것으로 나타났다. 이러한 결과로 보아 많은 생리활성물질 및 항산화물질이 함유되어진 김치의 섭취는 피부노화를 방지할 수 있을것으로 기대되며 특히 갓김치의 효과가 우수한 것으로 나타났다. Skin is the most frequently exposed tissues to oxidative stress from exogenous and endogenous sources. Dietary antioxidants, which suppress oxidative stress including reactive oxygen metabolites, play an important role in protecting skin from deleterious reactive oxygen species. Kimchi containing lots of antioxidative compounds shows anti-aging effect on skin. Therefore the morphologic changes on the skin of hairless mice fed diets containing Korean cabbage, mustard leaf, and buchu kimchi for 16 weeks were determined. Although epidermal thickness was decreased with age, kimchi prevented this thinning of epidermis compared to control group. In kimchi groups, the staining area of cytokeratin was smaller and stratum corneum was thinner than control group. It suggests that various kinds of kimchi diets prevent the increase of keratinization in epidermis with aging. Type Ⅳ collagen, a major structural protein of basement membrane supporting matrices, existed greater amount in kimchi groups than control group, especially in mustard leaf kimchi group. Rough endoplasmic reticulum (RER) of fibroblast was well developed in dermis of Korean cabbage and mustard leaf kimchi groups, which means collagen synthesis at dermis increased in those kimchi groups. This morphological changes of skin suggest that kimchi consumption can retard skin aging due to the presence of antioxidant and anti-aging compounds, especially some components of mustard leaf kimchi may largely affect on the skin rejuvenescence.

김치 종류를 달리한 식이가 Hairless Mice의 간과 피부에서 산화억제 및 광산화에 미치는 영향

류복미(Bog-Mi Ryu),류승희(Seung-Hee Ryu),이유순(Yu-Soon Lee),전영수(Young-Soo Jeon),문갑순(Gap-Soon Moon) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2

김치에는 여러 가지 항산화 물질들이 많이 함유되어 있으므로 김치의 섭취는 생체조직의 노화 억제 효과가 있을 것으로 기대된다. 본 연구에서 배추김치, 갓김치, 부추김치를 동결건조하여 항산화물질의 함량을 측정한 결과 갓김치와 부추김치의 클로로필 함량이 가장 높았고 β-카로텐과 ascorbic acid 함량은 갓김치에서 가장 높았다. Hairless mouse에게 10% 동결건조 김치식이를 공급하여 20주간 사육한 후 간에서 활성산소종의 생성량을 측정한 결과 superoxide anion과 hydroxyl radical 함량은 유의적인 차이가 없었고 hydrogen peroxide 함량은 갓김치군과 부추김치군에서 유의적으로 낮게 나타나 이들 김치가 활성산소를 소거하는데 효과가 있는 것으로 나타났다. 간 균질액에서 지질과산화물을 측정한 결과 대조군에 비해 김치섭취군에서 지질과산화가 낮게 나타났으며, 특히 갓김치와 부추김치군의 효과가 우수하였다. 피부에서 지질과산화물 함량은 김치섭취군들이 대조군보다 낮게 나타났으며, 부추김치가 유의적으로 지질과산화를 억제하였다. 김치섭취가 광산화에 미치는 영향을 규명하기 위해간 및 피부조직에 자외선을 조사한 후 조직산화 정도를 측정한 결과 간과 피부의 지질과산화물 함량은 갓김치군과 배추김치군에서 대조군보다 낮아졌으나 부추김치군에서는 높게 나타났다. 즉, 자외선 조사 후 갓김치군과 배추김치군은 대조군보다 광산화가 억제되었으나 부추김치군에서는 산화가 촉진되었다. Kimchi contains various antioxidative components, therefore it is expected that kimchi have the inhibitory activity on aging. Among the many factor related to aging, UV radiation is the easiest way to induce skin aging. This experiment was designed to demonstrate the antioxidative activity of dietary kimchi with and without UV radiation in tissues. Lyophilized Korean cabbage kimchi, mustard leaf kimchi and buchu kimchi were used for measurement of the contents of antioxidative components. Chlorophyll contents in buchu and mustard leaf kimchi were higher than cabbage kimchi, and the contents of carotene and ascorbic acid were higher in the mustard leaf kimchi. To clarify the antioxidative activity of various kimchi diets in vivo system, hairless mice were fed the diet containing 10% lyophilized kimchi for 20 weeks. Contents of superoxide anion and hydroxyl radical in liver were not shown significant differences, however hydrogen peroxide were significantly decreased in mustard leaf and buchu kimchi diet groups. Supplementation of all kinds of kimchi diets attenuated the hepatic MDA accumulation, especially in mustard leaf and leek kimchi group. In the skin, the contents of MDA were decreased in kimchi groups compared to control group, especially the buchu kimchi diet significantly inhibited the lipid peroxidation. To elucidate the effect of kimchi diets on photooxidation, the extent of oxidative damage in liver and skin was measured after UVB radiation treatment. The contents of lipid peroxides was decreased in mustard leaf kimchi group and cabbage kimchi group compared to control, while lipid peroxidation was increased in buchu kimchi diet group. It suggests that chlorophylls and other antioxidative nutrients in the various kimchi diets are related to antioxidation or photooxidation and it might be needed further study to certify the exact mechanisms.

Study of Thickness Estimation for the Interfacial Layer of TiO₂ ALD films

Gye-Choon Park(박계춘),Seung-Hee Yu(류승희),Kil-Ju Na(나길주),Soon-Youl So(소순열),Chae-Joo Moon(문채주),Jin Lee(이진),Yong Jun Park(박용준) 대한전기학회 2013 대한전기학회 학술대회 논문집 Vol.2013 No.7

Streptozotocin - 유발 당뇨쥐에서 부추식이의 산화적 스트레스 및 Lipofuscin 생성 억제 효과

이점옥(Jum-Ok Lee),류승희(Seung-Hee Ryu),이유순(Yu-Soon Lee),김정인(Jung-In Kim),문갑순(Gap-Soon Moon) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.8

산화적 스트레스를 많이 받는 당뇨상태에서 항산화활성이 높은 부추의 섭취가 지질 및 단백질 산화, 활성산소종의 생성, 항산화 효소계 활성 변화 및 lipofuscin 생성에 미치는 영향을 조사하고자 S.D.계 쥐에게 streptozotocin을 복강투여하여 당뇨병을 유발시킨 후 대조군 식이와 10% 부추식이를 14주간 섭취시켰다. 부추의 섭취는 대조군 식이에 비해 간보다는 피부조직에서 지질 및 단백질 산화를 유의적으로 감소시켰고(p<0.05), 반응성이 큰 활성산소종인 hydroxyl radical의 생성도 당뇨군에 비해 유의적으로 감소된 것으로 나타났다(p<0.05). 부추의 섭취는 간과 피부조직의 항산화 효소계 활성을 증가시키는 것으로 나타났으며 심장과 눈 조직에서 노화색소인 lipofuscin을 측정한 결과 당뇨군에 비해 당뇨부추군에서 감소하였고 특히 눈에서 유의적으로 감소하였다(p<0.001). 이상의 결과로 부추의 섭취는 당뇨 합병증을 일으키는 주요 원인인 산화적 스트레스를 억제하는 효과를 나타내었고, 당뇨 합병증의 증상완화에 도움을 줄 것으로 기대되어진다. Diabetes mellitus has been known to be a state of increased oxidative stress. Free radical formation and lipid peroxidation are accelerated in this metabolic disorder. Buchu (Allium tuberosum Rottler) contains lots of antioxidative nutrients such as chlorophyll, vitamin C, β-carotene, phenolic compounds and sulfur compounds. To investigate the protective effects of buchu, 10% lyophilized buchu diet was fed to streptozotocin (STZ)-induced diabetic rats for 14 weeks and lipid peroxidation, protein oxidation, contents of reactive oxygen species, activities of antioxidative enzymes and contents of accumulated lipofuscin were measured as indicators of oxidative stress. Hepatic MDA and carbonyl contents tended to decrease in 10% buchu diet group compared with control group. Dietary buchu significantly suppressed lipid and protein oxidation in the skin of rats (p<0.05). Contents of hepatic hydroxyl radicals, which exert the highest toxicity among the reactive oxygen species, were significantly decreased in rats fed 10% buchu diet (p<0.05). Activities of antioxidative enzyme, such as superoxide dismutase, catalase, and glutathione peroxidase, tended to increase in liver and skin of rats fed 10% buchu diet, while hepatic catalase activity was significantly increased in buchu group compared with control group. Buchu supplementation significantly inhibited the accumulation of lipofuscin, an end-product of lipid peroxidation reactions induced by reactive oxygen radicals, in eye tissues compared with control diet (p<0.001). In conclusion, buchu supplementation diminished the oxidative stress, so dietary buchu could help to attenuate diabetes complications.

홀스타인종 젖소에 있어서 PCR과 ELISA기법을 이용한 BLV 감염진단

정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),서국현(Guk-Hyun Shu),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1

This study was conducted to investigate the farm situation about bovine leukemia virus(BLV) infection that greatly influence productivity in dairy cattle and compare the accuracy of diagnosis for BLV infection between PCR and ELISA techniques. Blood samples of 193 heads from 5 herds in Chungnam and Chungbuk area were used to analyze BLV gene and serum, and the results were obtained as follows. The amplified BLV gene in dairy cattle by PCR technique resulted in 226 bp, 596 bp and 434 bp, respectively, for gag, pol and env, which were well amplified. The infection rates of BLV virus diagnosed by PCR and ELISA techniques ranged from 80.55 to 100% and from 22.22 to 86.95%, respectively, and the infection rates among 5 herds were significantly different in both methods (P<0.05). Further, the average infection rates of 5 herds were 87.05 and 63.21%, respectively, for PCR and ELISA techniques. Kappa statistics for examining consistency of diagnosis by PCR and ELISA techniques showed 0.246, which represents low consistency. Consequently, PCR based BLV technique was considered as a corrective measure for diagnosis of BLV infection in Holstein dairy cattle.

한국형 홀스타인종 젖소의 BLV env 유전자의 특성분석

정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),서국현(Guk-Hyun Suh),류승희(Seung-Heui Ryoo),정상일(Sang-Il Chung),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2

This study was performed to investigate the characterization of infectious BLV env gene isolated form Korean Holstein Cattle and to determine its incoming origin. Gp51 region of BLV env gene known as having important role in immunological function was characterized using PCR-RFLP sequencing and phylogenetic analysis. BLV env gene was grouped into PCR-RFLP patterns with three restriction endonucleases including Pvu Ⅱ, BamHI and HaeⅢ, and we identified two new RFLP patterns from nucleotide sequences of each group. Phylogenetic analysis showed that 80% of the Korean Holstein was included in the USA and Japanese group. These results here can provide a valuable information about the character of the BLV env gene and research on infection route of BLV.

홀스타인종과 한우에 있어서 BoLA-DRB3 유전자의 단일염기다형과 반수체 분석

정행진(Hang-Jin Jeong),유성란(Seong-Lan Yu),라세둘(M. R. Hoque),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.1

BoLA (bovine leukocyte antigens) have been known as gene complex related with bovine diseases and immunological traits. This study was conducted to find out the characteristics of BoLA-DRB3 gene related to mastitis and BL(bovine leukocyte) from 280 cattle [193 animals of Holstein cattle and 87 animals of Hanwoo]. As a result, five PCR-RFLP types (b, d, e, f and g) using HaeⅢ restriction enzyme, three BstYI restriction patterns (b, d and e) and eight RsaI restriction types(b, d, f, I, j, n, o and w) were identified. Moreover, we identified new d' type (197→175/22), having one more cutting site by BstYI enzyme than d type allele and n' type (180→169/11) having one more cutting site by RsaI enzyme than n allele was additionally identified. Next, we identified 53 SNPs in BoLA-DRB3 exon2 from 280 cattle. SNP frequency and heterozygosity of Holstein and Hanwoo were investigated in all the SNP genotype. These results might be based on research for identifying marker associated with bovine diseases.

한국 Holstein종 유우집단의 DGAT1 유전자의 특성분석

손지영(Ji-Young Son),정행진(Hang Jin-Jeong),유성란(Seong-Lan Yu),이준헌(Jun-Heon Lee),도창희(Chang-Hee Do),류승희(Seung-Heui Ryoo),상병찬(Byung-Chan Sang) 충남대학교 농업과학연구소 2009 농업과학연구 Vol.36 No.2

본 연구는 한국형 Holstein종 젖소집단의 DGAT1 유전자의 특성을 구명하고, DGAT1의 유전적 다형과 산유형질인 유량 및 유지량 간의 연관성을 구명하여 젖소집단의 유전적 개량을 위한 분자유전학적 접목을 위하여 실험을 실시하였다. Holstein종의 genomic DNA로부터 PCR기법을 이용하여 DGAT1 유전자좌를 specific primers로 증폭한 후, 1.5% agarose gel에 전기영동한 결과 411 bp의 단편이 양호하게 증폭되었음을 확인하였다. DGAT1 유전자의 염기서열을 분석한 결과 DGAT1 Q 대립유전자의 216-218 bp의 염기서열이 AUG(lysine, K)였으나, 동위치의 대립유전자 q의 염기서열은 GCG(alanine, A)로 치환되어 있음을 확인할 수 있었다. 한편 한국 Holstein종과 NCBI에서 보고된 bovine DGAT1 유전자 단편의 염기서열간에는 100% 상동성을 보였다. 한국 Holstein종 유우집단의 DGAT1 유전자형의 분포는 DGAT1 QQ, Qq 및 qq 유전자 분포가 각각 16.43, 36.43 및 47.14%로 qq 유전자형빈도가 다른 유전자형에 비하여 높았으며, 유전자빈도는 DGAT1 Q 및 q 빈도가 각각 0.35 및 0.65로 q의 빈도가 높았다. DGAT1 유전자형과 산유량인 유량 및 유지량간의 연관성에 있어서는 DGAT1 유전자형이 유량 및 유지량에서 유의적인 차이(P<0.05)를 보였으며, DGAT1 Qq 유전자형이 QQ 및 qq 유전자형에 비하여 유량과 유지량에서 유의적인 차이(P<0.05)로 높은 수치를 나타냈다. This study was conducted to characterize the DGAT1 gene in Korean Holstein dairy cattle population and examine the relationship of DGAT1 polymorphisms with milk yield and milk fat yield for the genetic improvement of Korean Holstein dairy cattle. Results indicated that the 411 bp PCR products were successfully amplified by DGAT1 specific primers. Sequence analysis indicated that the DGTA1 Q allele had AUG (Lysine, K) nucleotide sequences in 216-218 bp and q allele had GCG (Alanine, A) sequences in the same position. Nucleotide sequence homology between the DGAT1 sequences generated in this study showed 100% homology with bovine DGAT1 sequences in the NCBI database. The genotype frequencies of DGAT1 QQ, Qq, and qq were 16.43, 36.43, and 47.14%, respectively, in Korean Holstein dairy cattle population. The observed Q and q allele frequencies were 0.35 and 0.65, respectively. Statistically significant (P<0.05) results were identified for milk yield and milk fat yield for the DGAT1 genotypes. The Qq genotype Holsteins have significantly higher milk yield and milk fat yield than those of the QQ and qq genotype Holsteins(P<0.05).