정신분열병 환자에서의 혈중 Homocysteine 농도와 MTHFR C677T, A1298C의 유전자 다형성

이영식,전창무,나철,채석래,Lee, Young Sik,Jeon, Chang Moo,Na, Chul,Chae, Seok Lae 대한생물정신의학회 2005 생물정신의학 Vol.12 No.2

Objectives:Recently in schizophrenia high incidence of MTHFR(methylenetetrahydrofolate reductase), which is a main relating enzyme that reduce homocysteine level, genetic variations were reported. So we examined serum homocysteine level and MTHFR gene polymorphism in Korean schizophrenics. Method:We compared serum homocysteine level and MTHFR polymorphism between 235 schizophrenics (100male, 135female) and 235 normal controls(100male, 135female). C677T and A1298C polymorphism of MTHFR gene were analyzed. Results:1) C677T genetic mutation(CT and TT) were more frequent in schizophrenia group than normal control group(p<0.01). But the difference of A1298C mutation frequency was not found between two groups. 2) In schizophrenia patients, TT genotype of C677T mutation showed significantly higher homocysteine level (29.99uM/L) than other group(CT:13.34uM/L, CC:9.34uM/L p<0.01). 3) MTHFR 677 TT homogeneous mutation genotype showed two times more risk(odds ratio=2.15) than 677CC normal genotype in schizophrenia. Conclusion:Some schizophrenia patients with high homocysteine serum level may have C677T TT genotype. In that case, folate ingestion could be a good management for clinical improvement.

단기간의 참선수행이 갖는 정신 치료적, 생리적 효과

정성훈(Seong Hoon Jeong),채석래(Seok Lae Chae),정인원(In Won Chung) 동국대학교 불교문화연구원 2007 佛敎學報 Vol.0 No.46

악성 및 양성 담도질환에서 종양표지자로써 담즙내 CEA(carcinoembryonic antigen) 측정치의 비교분석

오정식(Jung Sik Oh),채석래(Seok Lae Chae),이정효(Jung Hyo Lee),김상준(Sang Jhoon Kim) 대한외과학회 1995 Annals of Surgical Treatment and Research Vol.49 No.2

한국 정신분열병 환자에서의 혈중 Homocysteine, 엽산, Vitamin B12 농도 비교연구

김태호,이영식,송성용,민경준,기백석,나철,채석래,Kim, Tae Ho,Lee, Young Sik,Song, Seong Yong,Min, Kyung Joon,Kee, Baik Seok,Na, Chul,Chae, Seok Lae 대한생물정신의학회 2004 생물정신의학 Vol.11 No.2

Objective:There have been a kind of transmethylation theory that high homocysteine serum concentration affects schizophrenia by neurotoxic mechanism and clinical reports that some schizophrenic patients with high homocysteine were improved by high folate ingestion. This study was done to confirm previous research results and find the clinical characteristics of schizophrenia showing high serum homocysteine and low folate. Method:We compared the serum levels of homocysteine, folate and vitamin B12 level between 234 schizophrenic patients(male 99, female 135) group and 234 normal controls(male 99, female 135) group. The subjects of two groups were age and sex matched. The evaluated clinical characteristics items were sex, age, onset of disease, hereditary loading, disease course, hallucination and subtype of schizophrenia. Results:1) Homocysteine level of the schizophrenia group was significantly higher than the normal control group and folate level of the schizophrenia group was significantly lower than the normal control group. Homocysteine level was more negatively correlated with folate level in the schizophrenia group than the normal control group. 2) The percentage of high homocysteine(above 12.46umol/L;90 percentile of normal control) was 33.8% of schizophrenia patients and 51.5% of male schizophrenia. The percentage of low folate(below 3.8nM/L;bottom tertile of normal control) was 66.2% of schizophrenia. 3) In low folate group and not-low folate group, schizophrenia showed significantly higher homocysteine level than normal control. Especially, low folate schizophrenia group showed significantly higher homocysteine level than low folate normal control group. Conclusions:Some schizophrenia patients with high serum homocysteine may be genetic defector and having low folate serum level. In that case, folate ingestion could be a good management for clinical improvement.

Two vivax malaria cases detected in Korea

Seung-Yull Cho(초승열),Yoon Kong(공윤),Sill Moo Park(박실무),Joon Seung Lee(이준승),Young Ae Lim(임영애),Seok Lae Chae(채석래),Weon-Gyu Kho(고원규),Jong Soo Lee(이종수),Jae Chul Shim(심재철),Hak-Kyoon Shin(신학균) 대한기생충학열대의학회 1994 The Korean Journal of Parasitology Vol.32 No.4

Sysmex SE-9000의 Morphologic Flag 분석 및 재검토조건 설정 : Evaluation on the Morphologic flags and Determination of the Review Criteria

채석래,박장순,김대철,김성원,차영주 대한임상병리학회 2000 대한임상병리학회지 Vol.20 No.5

Gel Test에 의한 불규칙항체 선별검사의 유용성

채석래,방광환,장은아,차영주 大韓輸血學會 1998 大韓輸血學會誌 Vol.9 No.1

인공물질의 혈액적합성 평가방법중 Platelet Factor 4의 유용성에 관한 연구

채석래,조한익,이동호,전희선,차영주,박성섭,한동근,김영하 大韓血液學會 1993 大韓血液學會誌 Vol.28 No.2

혈액질환에서 세포유전학적 이상

채석래,차영주 중앙대학교 의과대학 의과학연구소 1998 中央醫大誌 Vol.23 No.3

1991년 3월부터 1998년 7월까지 중대 부속 용산병원 임상병리과에 의뢰된 혈액질환 환자 및 림프종의 골수 전이 여부를 알고자 했던 환자 218명의 골수 흡인 검체를 대상으로 고정도분염법에 의한 염색체 검사를 실시하여 다음과 같은 결과를 얻었다. 1. 급성골수성백혈병 40예 중 23예(58%)에서 염색체 이상소견을 보였으며, 중요한 염색체 이상으로는 8;21 전좌(4예), 15;17 전좌(3예), 11번 전좌(2예), 5번 결실(1예), 7번 결실(4예), 8번 획득(2예) 등이 있었으며, 3개 이상의 염색체 결함이 있는 경우가 3예 있었다. 2. 림프구성 백혈병 15예 중 7명(47%)에서 염색체 이상소견을 보였으며, 중요한 염색체 이상으로는 9;22 전좌(3예) 및 과배수성 염색체(1예)가 있었다. 3. 만성골수증식성질환 20명 중 만성골수성백혈병 7명 모두에서 9;22 전좌가 있었다. 4. 골수이형성증후군 16예 중 10예(63%)에서 염색체 이상소견을 보였으며, 특히 RA에서의 염색체 결함의 빈도가 대체로 높았다. International prognostic scoring system에 따른 염색체 예후양호군은 6예, intermediate group은 7예, 불량군은 3예이었으며, 주요 결함으로서 5번 결실(2예), 7번 결실(1예), 8번 획득(3예) 및 5;22 전좌 1예등이었다. 5. 다발성골수종, 림프종 및 기타 혈액질환 환자에서는 특이한 염색체 결함은 발견되지 않았다. 이상과 같은 결과로서 염색체 검사는 혈액질환의 진단 및 예후결정에 중요한 역할을 한다고 사료되지만 염색체 제작과정이 까다롭고 시간이 걸리며, 판독자의 실력에 따라 판독에 차이가 있을 수 있으므로 많은 경험을 쌓아야 하며, 분자생물학적 진단을 병행한다면 감도 및 특이도를 더 높일 수 있으리라 사료된다. Cytogenetic analyses of 218 patients with hematologic diseases and lymphoma were performed by MTx-treated high-resolution banding technique and the results were as follows. 1. Twenty three cases (58%) of 40 acute myelogenous leukemias revealed cytogenetic defects, and main defects were t(8;21), t(15;17), t(11q23;variable), -5, -7/del(7q), +8, and complex defects. 2. Seven cases (47%) of 15 lymphocytic leukemias revealed cytogenetic defects, and main defects were 5(9;22) and hyperdiploid chromosome. 3. In 20 cases of chronic myeloproliferative disorders, all of 7 chronic myelogenous leukemia had t(9;22). 4. Sixteen cases(63%) of 16 myelodysplastic syndromes revealed cytogenetic defects, and main defects were del(5q), -7, +8, and 5(5;12). The frequency of cytogenetic defects in the refractory anemia appeared high. According to the international prognostic scoring system(IPSS), 6 cases were classified as good, 7 cases as intermediate and 3 cases as poor karyotype group. 5. Other hematologic disease, lymphoma or multiple myeloma cases did not have disease-specific cytogenetic abnormalities. In conclusion, cytogenetic study plays an important role in the diagnosis and differential diagnosis of hematologic diseases as well as prediction of prognosis, and molecular study together with cytogenetic interpretation by skilled expert would raise sensitivity and specificity in the detection of genetic defects.

자동화 매독 검사법인 Architect Syphilis TP assay와 Mediace TPLA의 일치율 및 위양성 평가

허희진,채석래 동국대학교 의학연구소 2009 東國醫學 Vol.16 No.1

자동화장비로 검사 가능한 Mediace Treponema pallidum latex agglutination (Sekisui, Japan; 이하 Mediace TPLA)와 Architect Syphilis TP assay (Abbott, Germany; 이하 Syphilis TP)의 결과치를 비교하여 일치율을 조사하였다. 트레포네마검사에서 위양성의 가능성이 높은 환자 검체를 대상으로 두 검사법의 위양성률을 구하여 두 자동화검사법의 유용성을 평가하고자 하였다. Mediace TPLA 양성이었던 161 예를 대상으로 Syphilis TP 검사를 시행하였다. 위양성률을 평가하기 위해 항핵항체 양성이거나 B형간염표면항원 양성, 항-C형간염바이러스항체 양성, rheumatoid factor 양성, CRP 양성 또는 70세 이상의 고령 환자 검체 234예를 연구 대상으로 Mediace TPLA와 Syphilis TP 검사를 시행하였다. 두 검사 결과의 차이를 보이는 예는 line-blot immunoassay (Innolia Syphilis score, Innogenetics, Belgium)로 확인하였다. Mediace TPLA 양성이었던 161예중 4예 (2.5%)는 Syphilis TP 음성이었고, 이 중 2예는 line-blot immunoassay 양성, 2에는 음성이었다. 중화후 Mediace TPLA, 중화전 Mediace TPLA와 Syphilis TP의 위양성률은 각각 0%(0/234), 1.7%(4/234), 1.7%(4/234)였다. 두 검사의 일치율은 98%(387/395) 였다. 자통화 매독검사인 Mediace TPLA와 Syphilis TP는 양성으로 나온 검체에서의 일치율이 높고 기존의 트레포네마검사 보다 낮은 위양성률을 보이므로, 수기법을 대체할 수 있는 유용한 검사로 생각된다. We compared the results of Mediace Treponema pallidum latex agglutination (TPLA) (Sekisui Chemical Co., Ltd, Japan) with Architect Syphilis TP assay (Syphilis TP) (Abbott, Germany), automated treponemal tests for the diagnosis of syphilis. To establish false positive rates, two methods were tested with patients' sera with laboratory results reported to be related with false positivity of treponemal test. One hundred sixty one patients' sera with Mediace TPLA positivity underwent Syphilis TP. Two hundred thirty four patients' sera with positivity for anti-nuclear antibody, HBs Ag, Anti-HCV, rheumatoid factor or CRP, or old age (≥70) were included in the evaluation of false positive rates. Discrepancies between Mediace TPLA and Syphilis TP results were confirmed by line-blot immunoassay (Innolia Syphilis score, Innogenetics, Belgium). Among 161 patients' sera with Mediace TPLA positivity, 4 cases (2.5%) showed discrepancy between Mediace TPLA and Syphilis TP results: Among 4 cases, 2 cases showed positive line-blot immunoassay results and 2 showed negative results. False positive rate of Mediace TPLA after neutralization, Mediace TPLA before neutralization and Syphilis TP was 0.0% (0/234), 1.7% (4/234) and 1.7% (4/234), respectively. The concordance rate of Mediace TPLA and Syphilis TP was 98% (387/395). The results of comparison data demonstrated that Mediace TPLA and Syphilis TP had high concordance. False positivity of Mediace TPLA and Syphilis TP were low compared with previously reported that of treponemal test. Therefore, automated treponemal tests would be considered to be a valid alternative to traditional treponemal test for confirmation of syphilis.