벼 葯培養 分化植物體의 變異性 Ⅱ. 量的形質의 變異

Sae Jun Yang(陽世準),Byeong Geun Oh(吳秉根),Gun Sik Chung(鄭根植),Jae Keun Sohn(孫再根) 한국육종학회 1987 한국육종학회지 Vol.19 No.4

The present study was focused on variability of anther-derived plants in quantitative characters to obtain basic information for rice breeding through anther culture. Variability of progeny of anther-derived plants, A₂ Lines, were compared with F₂ generation of the same combination. The salient findings obtained are summarized as follows; The segregation and recombination of major characters such as days to heading, panicle length, and panicles per plant both 175 anther-derived A₂ lines and 594 F₂ plants derived from Milyang 74/Dashukei 2 F₁ hybrids presented normal distribution curves and refered to the inheritance of quantitative characters controlled by multiple genes. Expression of gene recombinants related to culm length of 48 anther-derived A₂ lines from singwangbyeo/Eunhatyeo F₁ hybrids showed similar to those of 406 F₂ plants, although average culm length of A₂ population was shorter than that of F₂ population. The segregation and recombination of fertility of 71 anther-derived A₂ lines from Milyang 62/Akibare F₁ hybids as remote cross showed clearly different from those of 578 F₂ plants. The curve of A₂ population appeared more centered, and tilted to higher fertility. This tendency was also appeared both anther-derived A₂ lines and F₂ population derived from Singwangbyeo/Eunhabyeo F₁ hybrids.

벼 組織培養을 利用한 突然變異誘發 硏究 Ⅱ. 半數體유래 變異體의 出現頻度의 短稈變異體의 遺傳分離

Sae Jun Yang(楊世準) 한국육종학회 1990 한국육종학회지 Vol.22 No.2

This experiment was conducted to broaden the scope of mutation breeding through in vitro culture techniques. Phenotypic variation between A₂ lines derived from rice anther culture (CV, Dashukei 2) and M₂ lines derived from haploid node culture with mutagen, DES(Diethylstilbestrol) 10⁻⁵ Mol, were compared in major agronomic characters. Genetic analysis of a induced variants with stunted culm from haploid node culture with mutagen, DES, was also conduced to clarify the mode of inheritance. This was for the first time confirmed in androgenic somacolonal variants derived from haploid cell culture with mutagen. Although almost of A₂ and M₂ population were nearly same to parental variety, the frequency of lines without within-line segregation was 4.8% in anther-derived A₂ population and 19.2% in haploid node culture-derived M₂ population in phenotypic patterns. The frequency of lines with simple segregation in one or two characters was much greater in M₂ population than A₂ population. It may suggest that extent of androgenic somaclonal variation occurred in vitro process will be increased by supplemented with mutagen. A induced mutants with stunted culm and panicle length, about 87% of culm length and 81% of panicle length compard to parental variety, could be selected for genetic analysis from haploid node culture-derived M₂ population. The segregation of 505 F₂ plants and 76 BC₁F₁ plants from a cross between Dashukei 2 as parental variety and Dashukei 2 M28 with stunted culm presented nearly normal distribution curve and referred to the inheritance of a quantitative character controlled by multiple genes. Molecular evidence for explanation of androgenic somaclonal mutants should be needed in the near future.

벼 葯培養 分化植物體의 變異性 Ⅲ. 質的形質의 變異

Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Gun Sik Chung(鄭根植),Jae Keun Sohn(孫再根) 한국육종학회 1988 한국육종학회지 Vol.20 No.1

The present study was focused on variability of qualitative characters in anther-dervied plants to obtain basic information for rice breeding through anther culture. Variability in the progeny of antherderived plants, A₂ lines, were compared with F₂ generation of the same cross combination. The frequency of tall in culm length was 79% in 594 F₂ plants, but 56% in 175 A₂ lines derived from Milyang 74/Dashukei 2 F₁ hybrids. The ratio of tall to short was 3:1 in F₂ population, and 1:1 in A₂ lines. Short culm of Milyang 74 was controlled by a recessive gene. There was no variants related to glaborous leaf blade of Dashukei 2 and resistant reaction to bacterial leaf blight, pathotype K₁, of Milyang 74 in A₂ lines. The glaborous leaf blade blade of Dashukei 2 controlled by a recessive gene and resistant reaction to bacterial leaf blight, pathotype K₁, of Milyang 74 controlled by a dominant gene were domonstrated in both F₂ population and A₂ lines derived from Milyang 74/Dashukei 2 F₁ hybrids.

벼 극만식재배에서의 赤枯發生과 그 遺傳

양세준(Sae Jun Yang),이수관(Soo Kwan Lee),정근식(Gun Sik Chung) 한국육종학회 1992 한국육종학회지 Vol.24 No.3

Rice leaf discoloration in latest season planting under field condition proposed as a screening method for cold tolerance and its mode of inheritance detected here. The salient findings were summarized as follows; Rice leaf discoloration in latest season planting showed from the begining of cool temperature below margin to just before frost falls under field condition at adult stage. Components value, showing the dispersion of population, in rice leaf discoloration of 44 genotypes at weekly reading under two different condition indicated appropriate time to distinguish varietal difference. Highly significant correlation(r=0.7536) recognized between adult stage in latest season planting under field and seedling stage under cool water tank. Clear difference in rice leaf discoloration recognized among varietal groups. Indica having cold tolerance and Tongil type rice showed more greater degree and variance value in leaf discoloration than that of Japonica rice. Any significant varietal difference in leaf discoloration could not recognize in the Japonica rice. Based on the screening test, Milyang 83 as susceptible parent, and Iri 371, IR20654-RRR-15-1 as resistant parents were selected, then crossed reciprocally to detect inheritance mode of leaf discoloration. The mode of inheritance for rice leaf discoloration revealed that resistant characteristics was showed to be partial dominance, and the maternal effects could not be recognized.

벼 葯培養 分化植物體의 變異性 Ⅳ. 통일형 / 자포니카형 F₁ 葯培養系統의 分類

Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Soo Kwan Lee(李壽寬),Gun Sik Chung(鄭根植) 한국육종학회 1989 한국육종학회지 Vol.21 No.1

Anthers of a cross between Tongil rice CV., Milyang 23 and Japonica rice CV., Nagdongbyeo were cultured on N6-Y1 medium, which Japonica rice was better adapted than Indica, to confirm a certain degree of screening effect of culture medium on the genetic expression of pollen plants. The salient findings obtained are summarized as follows; The majority of progeny of pollen plants, A2 lines, had horizontal leaf angle as intermediated type between parental varieties. Most of A2 lines also had the same leaf color of their parental varieties, but transgressive variation was expressed. Although most of A2 lines in grain shape distributed within parental range, transgressive variation in grain shape was also observed. The phenol color reaction of brown rice was coincide with seed integuments. The ratio of postive(+) phenol reaction to negative(-) was 1:1 in A2 population. It was considered that inheritance of the phenol color reaction was controlled by single dominant gene. The coloration of phenol solution after soaking seed integuments in 2% phenol for 5 days at 30℃ was more clearly than in brown rice. Transgressvie variation to milyang 23 observed both brown rice and seed integuments. Based on the results described above, the pollen grain of different genotype probably have the same totipotency into intact pollen plants. No significant selection effects was found during the process of anther culture. This results also provide useful information of rice breeding for Indica/Japonica hybrids through anther culture.

벼 組織培養을 利用한 突然變異誘發 硏究 Ⅰ. 半數體에 대한 化學物質의 突然變異 誘起效果

Sae Jun Yang(楊世準),Byeong Geun Oh(吳秉根),Soo Kwan Lee(李壽寬),Gun Sik Chung(鄭根植) 한국육종학회 1988 한국육종학회지 Vol.20 No.3

This experiment was conducted to broaden the scope of mutation breeding and to introduce new source of variation by in vitro technology. Node tissue of haploid plants, derived from rice anther culture (CV. Dashukei 2) were cultured on the N6-Y1 basic medium supplemented with 10⁻⁵M NAA, 0.5 x 10⁻⁵M kinetin, and 5x10⁻⁵M ABA. Chemical mutagens, DES, EMS, Sodium Azide, and MNNG were applied in vitro system through this experiment. Diploidization of’haploid plants regenerated from node culture with mutagens were conducted. The progeny of regenerated diploid plants, M₂ generation, were evaluated in their major agronomic characters, and they were compared with A₂ generation derived from anther culture. Haploid plants directly regenerated from node tissue of haploid without callus formation. The ratio of plant regeneration was decreased when the concentration of chemical mutagens were increased. Autodiploidization ratio by tiller separation was 3.1% while that of 0.2% colchicine treatment for 12 hours was 23.0% under the field condition. Phenotypic patterns of 197 M₂ lines derived from node culture with mutagens showed that 80.2% M₂ lines were similar to their parental variety, but the others were different in some characters. For days to heading and culm length the means did not change but the range of variance was larger in M₂ lines than in parental variety. Distribution of days to heading in 146 M₂ lines derived from DES treatmat was similar to 137 A₂ lines derived from anter culture, but that of culm length between two populations showed a significant difference.

벼 葯培養에 있어서 低溫處理와 分化植物體의 倍數性

Sae Jun Yang(楊世準),Jae Keun Sohn(孫再根),Gun Sik Chung(鄭根植) 한국육종학회 1985 한국육종학회지 Vol.17 No.4

This experiment was studied to determine the effects of low temperature treatment on culturability and ploidy level of anther-derived plants in rice anther culture. N₆ basic medium supplemented with 2mg/ℓ of NAA and 1mg/ℓ of Kinetin used for callus induction, 0.2mg/ℓ of IAA and 1mg/ℓ of Kinetin for plant differentiation. Rice panicle with flag leaf sheath was pretreated by low temperature at 10℃ for 15 days. Cold-shocked anthers produced more callus induction and plant differentiation about 3 fold than those without pretreatment. Callus induced from cold-shocked anthers produced more haploid plants and less diploid plants than the callus induced from anthers without pretreatment. This tendency was recognized in a variety as well as some F₁ crosses.

벼에서 캘러스 유기원와 계대배양 기간에 따른 재분화 계통의 주요 농업형질 변이

이기환,오병근,양세준,김순철,남민희,Yi, Gi-Hwan,Oh, Byeong-Geun,Yang, Sae-Jun,Kim, Soon-Chul,Nam, Min-Hee 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4

벼의 미수분 자방배양으로부터 분화된 후대집단 (0$_2$)의 주요 농업적 형질특성을 조사하고 이를 약배양 및 현미배양으로부터 분화된 후대집단과 상호비교하여 본 바, 주요농업형질의 계통내 고정도는 자방배양에서 87.9%,약에서는 89.8%,현미배양은 82.6%로 나타나 약배양과 마찬가지로 자방배양에서도 고정도가 높았다. 주요 형질별 분리양상을 보면 자방배양에서는 엽색이 4.2%로 가장 빈도가 높았으며, 약배양에서는 임성 (6.1%),현미배양에서는 엽색 (4.9%),임성 (4.9%)순이었다. 또한 캘러스를 1달과 2달 계대배양한 후대의 경우 간장에서 각각 8.9%와 10.0%로 분리하는 형질의 계통비율이 높게 나타났다. 간장, 수장, 출수기에 대한 변이성 정도는 자방배양에서는 약배양과 비슷한 정도의 변이를 보인 반면, 현미배양에서는 크게 나타났으며 간장과 수장의 평균은 모두 모품종인 화영벼에 비하여 줄어들었고 출수기는 다소 지연되는 경향을 보였다. Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17%) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1%) in ACP, leaf color (4.2%) in OCP and grain fertility (4.9%) and leaf color (4.9%) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).

嶺南地域 乾畓直播裁培適應 벼 品種選定

Byung Tae Jun(田炳泰),Sae Jun Yang(楊世準),Un Sang Yeo(呂運尙),Kee Yeong Lee(李基榮),Soo Kwan Lee(李壽寬) 한국육종학회 1993 한국육종학회지 Vol.25 No.4

This experiment was conducted to screen the adaptability of Korean rice cultivars to direct seeding on dry soil at Yeongnam Crop Experiment Station in 1992. Twelve rice cultivars, two of early maturity. four of medium maturity and six of medium-late maturity, were seeded from May 1 to June 20 with ten-day interval. Based on the minimum cumulative temperature for ripening, 760℃ for forty days after rice heading, the critical late seeding date were June 20 for early maturity varieties, June 10 for medium maturity ones, and May 30 for medium-late maturity ones. Especially, the critical late seeding date for Tamjiribyeo, a medium-late maturity rice cultivar, was May 20. Changes in agronomic characters by direct seeding as compared with transplanting should be checked on the basis of seeding date rather than transplanting date. There are no problems in germinability at fifteen days after seeding except Sangjubyeo, Hwaseongbyeo, although varietal difference of low temperature(15℃) germinability was clearly recognized at ten days after seeding. Degree of seedling emergence(1~9) at twenty days after May 1 seeding under the field condition was good in Dongjinbyeo, Yeongnambyeo, Tamjinbyeo, and Donghaebyeo, but it was relatively fair in Jinmibyeo, Hwaseongbyeo, and Ilpumbyeo. Seedling vigor at thirty five days after May 1 seeding also was very vigorous in Dongjinbyeo, but it was normal in Ilpumbyeo. Incidence degree of lodging in the field was more severe in direct seeding than in transplanting. Donghaebyyeo, Yeongnambyeo and Ilpumbyeo were evaluated as lodging tolerant cultivars. Significant negative correlation coefficient between field lodging and breaking strength was recognized regardless of cultivation method. Milled rice yield by direct seeding was nearly 94.8% level of that by transplanting at the same seeding time of May 10. The critical late seeding time of direct-seeded cultivation based on heading date can be explained by the relationship between the seeding time and grain yield. Donghaebyeo, Yeongnambyeo, Ilpumbyeo, Dongjinbyeo, and Tamjinbyeo were recognized as considerably adaptable rice cultivars to direct-seeded cultivation in southeastern plain area by the lodging tolerance and relative level of grain yield compared with transplanting.

벼 葯培養에서 캘러스 形成과 植物體再分化能力에 대한 遺傳分析

Jae Keun Sohn(孫再根),Sae Jun Yang(楊世準) 한국육종학회 1993 한국육종학회지 Vol.25 No.2

Inheritance of callus formation and plant regeneration in rice anther culture was studied using the diallel technique. Anthers containing uninucleate microspores from four cultivars(japonica ; “Chuncheongbyeo” and “Palgongbyeo”, Tongil ; “Chilseongbyeo”, indica ; “IR50”) and their F₁’s were cultured on N₆-Y₁ medium supplemented with 1.0㎎/ℓ NAA and 2.0㎎/ℓ kinetin. The one-step culture method of anthers using the culture bottle was applied for standardization of anther culture process in this study. The parents showed significant differences in frequency of callus formation, green plant regeneration and albino occurrence. General and specfic combining abilities were estimated and found to be highly significant for the three traits. Reciprocal effect was also significant for callus formation and albino occurrence, but it was not significant for green plant regeneration. Further analysis for green plant regeneration showed gene action to be overdominant with high response being highly recessive and controlled by a few genes. The estimates of narrow sense heritability for albino occurrence(0.32) was lower than those of callus fromation(0.76) and green plant regeneration(0.55).