국내 수집 야생버섯류 추출물의 생리활성 비교

안기홍,한재구,김옥태,조재한,An, Gi-Hong,Han, Jae-Gu,Kim, Ok-Tae,Cho, Jae-Han 한국버섯학회 2021 한국버섯학회지 Vol.19 No.1

국내 자생하는 야생버섯 추출물의 생리활성 성분을 평가하기 위하여 각지에서 수집된 야생버섯 70% 에탄올추출물에 대한 DPPH 라디컬 소거능, 총 폴리페놀 및 총 플라보노이드 함량, 철 환원 항산화능, 환원력의 항산화 활성, 아질산염 소거능 및 야생버섯의 건조시료를 이용하여 베타글루칸 함량을 분석하였다. 본 연구에서 수집된 10종의 야생버섯류 중에서 영지(OK1362) 에탄올추출물의 DPPH 라디컬 소거능(73.2%), 총 폴리페놀 함량(28.9 mg GAE/g) 및 총 플라보노이드 함량(10.0 mg QE/g)철 환원 항산화능(0.134), 환원력(0.155), 아질산염 소거능(56.3%)이 다른 버섯류에 비하여 유의적으로 가장 높았다. 그 이외에도 아까시흰구멍버섯(OK1360), 광대버섯속의 A. lanigera (OK1398), 졸각무당버섯(OK1406)이 높은 항산화능 및 아질산염 소거능을 가지고 있음을 확인하였다. 또한 베타글루칸 함량은 영지(OK1362)가 25.2%를 나타내며 가장 높았으며, 그 외에 구름버섯속의 T. lactinea (OK1457)가 24.5%로 높은 것으로 나타났다. 본 연구의 결과는 국내 자생하는 야생버섯류 중 새로운 천연물 유래 생리활성 물질을 탐색하기 위한 기초자료로서 활용 가능성을 기대한다. The aim of the study was to obtain the extracts of various native wild mushrooms and select the useful resources though biological activity evaluation. The anti-oxidant potential, nitrite scavenging activity, and ��-glucan content of wild mushrooms collected from Eumseong and Bonghwa in Korea were investigated. Based on the results of this study, Ganoderma lingzhi (OK1362) showed the highest DPPH radical scavenging activity (73.2%), ferric reducing anti-oxidant power (0.134), reducing power (0.155), nitrite scavenging activity (53.6%), total polyphenol content (28.9 mg GAE/g), flavonoid content (10.0 mg QE/g), and ��-glucan content (25.2%) when compared to other wild mushrooms sampled in this study. In addition, it was confirmed that Perenniporia fraxinea (OK1360), Amanita sp. (OK1398), and Russula sp. (OK1406) had relatively high anti-oxidant and nitrite scavenging potentials. In conclusion, our results can provide fundamental data for extracting beneficial compounds from wild mushrooms.

병풀 (Centella asiatica L. Urban) 식물체 배양을 이용한 Triterpene Glycoside 생산

김옥태,김민영,박윤정,홍민희,안준철,오만호,황백,Kim, Ok-Tae,Kim, Min-Young,Park, Yoon-Jung,Hong, Min-Hee,An, Jun-Chul,Oh, Man-Ho,Hwang, Baik 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4

기내에서 병풀의 식물체 배양을 확립하였고 기본배지 설정 및 대량원소들, sucrose 농도가 triterpene glycoside (Made-cassoside와 Asiaticoside: M&A) 생산에 대한 영향을 조사하였다. 병풀 식물체가 각각의 배지에서 배양되었을 때, MS와 RCM배지는 각각 식물체 생장과 M&A 생산에 대해 가장 좋은 결과를 나타냈다. 하지만, M&A에 대해 가장 높은 생산성은 B5 배지로 나타내었다. B5 배지의 대량영양소인 KNO$_3$는 25 mM, NaH$_2$PO$_4$는 1 mM, CaC1$_2$는 1 mM 그리고 MgSO$_4$는 1~10 mM에서 M&A 생산이 최적을 이루거나 증가시켰다. Sucrose를 농도별로 처리했을 때, 6% sucrose 농도에서 가장 높은 M&A 생산을 얻을 수 있었다. Whole plant cultures of Centella asiatica (L.) Urban in vitro were established and the effects of basal media, some macro elements and sucrose concentration on productivity of triterpene glycosides (madecassoside and asiaticoside: M$\varepsilon$A) were investigated. Among the media (MS, B5, RCM) tested, MS and 0.5 RCM medium were the best for plant growth and M$\varepsilon$A production, respectively. However, taking into account the M$\varepsilon$A productivity, B5 medium was superior (M$\varepsilon$A: 14.28 mg/g dry wt.). Major macronutrients of B5 medium adjusted with the concentration of 25 mM KNO$_3$,1 mM NaH$_2$PO$_4$, 1 mM CaCl$_2$ and 1~10 mM MgSO$_4$, caused elevated or optimized levels of M$\varepsilon$A. On sucrose concentration, the highest yields of M$\varepsilon$A were obtained from 6% sucrose.

갯기름나물의 체세포배 분화와 기관 분화를 통한 재분화

김옥태,김광수,안준철,황백,Kim, Ok-Tae,Kim, Kwang-Soo,An, Jun-Chul,Hwang, Baik 한국식물생명공학회 2001 식물생명공학회지 Vol.28 No.1

식용 및 약용으로 사용되는 갯기름나물의 기내배양을 통한 재분화율을 조사하였다. 캘러스는 잎과 엽병의 절편체에서 발생하였고 0∼5 mg/L의 NAA와 0∼10 mg/L BA가 첨가된 MS 기본배지에 치상하여 암배양 결과 자발적인 배 분화와 기관 분화를 보여주었으며 2.5 mg/L NAA와 10 mg/L BA의 조건에서 재분화율이 가장 높았다. 체세포 배의 발생양상은 구형, 심장형, 어뢰형을 거쳐 자엽 단계로 발달하여 전형적인 접합자 배의 모습으로 발달하였다. 배 발생한 일차 체세포배를 0.2 mg/L의 NAA가 첨가된 배지에서 배양하였을 때, 이차 체세포배가 발생하였으며 주로 일차 체세포배의 배축과 하배축에서 많이 발생하였고 그 다음으로 자엽이나 뿌리 부분에서 발생하였다. Peucedanum japonicum $T_{HUNB}$ used as a edible and medicinal plants was investigated for in uitro regeneration. Callus formation occurred on leaf and stem explant cultures and showed spontaneous embryogenic and organogenic capability on MS basal medium supplemented with 0.1~5 mg/L NAA and 0~10 mg/L BA in dark. The regeneration was highest on the condition supplemented with 2.5 mg/L NAA and 10 mg/L BA. Development of the somatic embryo progressed through the globular, heart-shaped, torpedo-shaped and cotyledonary stage, typical of zygotic embryos. When the first somatic embryos was cultured on the medium supplemented with 0.2 mg/L NAA, secondary somatic embryo were induced with higher frequency on the hypocotyl then on the cotyledon and root.t.

성경에 나타난 체육 -신체관, 스포츠, 여가관을 중심으로-

김옥태 ( Ok Tae Kim ) 기독교학문연구회 2012 신앙과 학문 Vol.17 No.2

이 논문은 신·구약 성경과 관련 문헌을 통하여 신체관, 스포츠, 여가관 등을 중심으로 성경에 나타나 있는 체육의 개념에 대해 고찰한 것으로서 결과는 대체로 다음과 같다. 첫째, 성경에서의 신체관은 피조의 영적 존재이다. 즉 인간은 하나님의 형상과 모양에 따라 창조된 육을 가진 존재이나 영혼도 소유하고 있다. 육체는 약하지만 무시할 것이 아니라 잘 관리할 책임도 있다. 일찍이 성경은 오늘날 체육의 개념인 심신일원론적이었다. 둘째, 성경에 나타난 스포츠는, 구약시대에는 주로 활, 칼, 창, 물매 등 전쟁과 관계있는 것과, 무용, 수영 등이 성행하였다. 신약시대에는 바울서신에 여러 비유로 나타나 있는데, 이로써 스포츠가 부정되지 않고 긍정적으로 평가되고 있다는 것을 알 수 있다. 셋째, 성경에서의 안식일은 인간의 일과 탐욕에 대한 규제와 하나님이 보인 만족이라는 측면에서 여가적인 의미를 찾을 수 있다. 축제는 하나님이 만든 일종의 놀이라 할 수있고, 특히 그리스도인은 예배를 통하여 축하와 기쁨과 자유를 누려야 하는데, 인간의 문화는 그것을 위축시키고 있다. 결국, 체육이 역사적으로 기독교에서 부정된 적이 많았지만, 성경에 나타나 있는 진정한 의미는 긍정적이며 오히려 권장되어야 하는 것으로 나타나 있는 것이다. This paper considered the concept of physical education in the Bible. The three topics presented were the view of the human body, sports and the view of leisure through the Bible. The results are as follows: First, the view of the body in the Bible, is that we are created by God as spiritual beings both in body and soul. Though the body is weak, we must not neglector the body, but we must manage it responsibily. In the Bible, one can identify similar ideas that are practiced in modern physical education, the Bible shares the same view as modern physical education. Second, in the Old Testament days, archery, fencing, spear and sling were all physical activities related to war, while dance and swimming were related to physical education and sports. In the New Testament days many metaphors are written in Pauline letters, it suggests that sports were not denied but were encouraged and venerated. Third, the Sabbath in the Bible encompasses the meaning of leisure, control of the human`s work ethics, greed and God`s satisfaction. Festivals are a sort of play God has made. Christians should enjoy celebrations, joy and freedom through worship, However human culture restricts these celebrations. Ultimately, though physical education has been denied in Christianity historically, the true meaning of physical education in the Bible is positive and encouraging message for all to practice and enjoy physical education.

여가스포츠 노인참여자의 생활양식과 건강증진행동 및 웰니스의 관계

김옥태(Kim, Ok-Tae),이승희(Lee Seung-Hee) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.4

The purpose of this study is to contribute to promoting elderly welfare, by empirically analyzing the relationship among lifestyle, health promotion behavior and wellness of elderly participants in leisure sports, in order to improve health and physical strength of elderly people as well as to help provide them with mental and emotional stability. With 397 people of elderly participants, this study has been analyzed using one-way ANOVA, multiple regression analysis and path analysis, and the results are as follows; First, there are significant differences in the level of wellness according to individual characteristics. Second, the relationship between lifestyle and health promotion behavior is statistically significant. Third, the relationship between lifestyle and wellness is statistically significant. Fourth, the relationship between health promotion behavior and wellness is statistically significant. Last, there are significantly causal relationships among lifestyle, health promotion behavior and wellness. Consequently, for the sake of elevating the level of wellness of elderly participants in leisure sports, a positive lifestyle need to be established, and instructors in leisure sports should make constant efforts to boost health promotion behavior of the elderly.

체육과 인간형성 : 그 가능성과 한계

김옥태(Ok Tae Kim) 한국체육철학회 2000 움직임의철학 : 한국체육철학회지 Vol.8 No.2

The purpose of this study is to consider the possibility and limitation of character formation in physical education through the review of related literatures. I mentioned first the object of character formation of physical education with a special emphasis on physical, mental, social factor, and considered the condition of character formation from two points of view-physical activity and personality, and the attitude in physical education. The conclusions of the possibility and limitation of character formation in physical education are as follows: First, heredity and environment are important factors for character formation and their roles have been argued. But, these two factors interact with complex forms and play an important role in human development. Second, we can take the character to a fixed limitation within the certain limits through the studying means and method at sport site. We can`t improve the physical fitness or motor skill, and mental and social factors which we expect above genetic limits. But, we can recognize the limits of that by the developmental and physiological limits, the limits of this by doing physical activity intentionally. Third, except sports site, the transfer of emotion and social character through physical activity, to actual life can`t be expected. But it is different by individuals. If we want the attitude learned from the sports site to be transferred to daily life, we should make special efforts for generalization; we should fix our eyes on the principle of similarity by `Identical Element Theory`.

일본 고령자의 스포츠활동에 대한 의식과 실태

김옥태(Kim, Ok Tae) 한국체육철학회 1998 움직임의철학 : 한국체육철학회지 Vol.6 No.1

The purpose of this study is to investigate the consciousness and actual condition for the sports activities of the aged in Japan that is a great economic power and the most long-lived country in all the world and highly developed in the field of social sports. The results analyzed from the data of the organs of government and society are as follows: 1. The major cause for the old people`s sports activities is founded on the promotion of health and physical fitness. 2. The major reasons that the aged don`t do sports activities were that they are merely old. 3. The men did sports activities more in the leisure than the women. 4. Men and women alike played swimming and gate ball in the sports activities of the latent demand of the leisure activities. 5. The men did sports activities a little in the arulual vacation. 6. It was walking, free gymnastics and gate ball that the aged do the sports activities mainly.

기독교 안식일과 여가

김옥태(Kim, Ok Tae) 한국체육철학회 2002 움직임의철학 : 한국체육철학회지 Vol.10 No.2

The significance of leisure in the Sabbath is great. The purpose of this paper was to explore the meaning of leisure in the Sabbath of the Holy Bible and in the Lord`s day(Sunday) by the times. The results of this study by the Old and the New Testaments and related literatures were summarized as follows: First, God`s rest in the Old Testament emphasized the leisure by setting the limit positively to the work and the avarice of human being, and provided us with the model for the rest and leisure of the man. Second, the rest as a commandment of the Old Testament told the rhythm between work and repose, namely, complement of two kinds. Third, Jesus of the New Testament had a good rest with prayer in spite of His tight schedule, and He encouraged His disciples to have their leisure. Fourth, the Sabbath of commandment of the Old Testament was focused on man`s life and dignity by jusus, and gave us the meaning of leisure. Fifth, the concept and the meaning of the Sabbath have been changed in the doctrine and leisure with times since the Bible age through the present age. Sixth, today Sabbatarianism was declined greatly with the age of mass leisure, but, on the other hand keeping the Sabbath was emphasized.

체육계열 대학생의 사회적 지지와 학교생활적응이 진로준비행동에 미치는 영향

김옥태(Kim Ok-Tae),이승희(Lee Seung-Hee) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.3

The purpose of this study was to examine the effects of social support and college life adjustment on career preparation behavior among college students in physical education department, in order to help them establish a systematic course of career and provide them with basic data for career preparation. The analysis has been conducted with the subject of 618 college students in department of physical education and the results are as follows; First, there were significant differences in the degree of career preparation behavior according to individual characteristics. Upper-year groups have higher degree of career preparation behavior. field-experienced students have higher degree of career preparation behavior. As for career ambition and career preparation behavior, PE majors have the highest degree of career search, and then come graduate students and non-majors in order. Graduate students have the highest degree of career counseling and target behavior. Then come PE majors and non-majors in order. Second, social support has significant effects on career preparation behavior. Third, college life adjustment has significant effects on career preparation behavior. Consequently, the college authorities and professors should continue their interest in and effort for enhancing social support and college life adjustment for the right career preparation behavior among college students in physical education department.

현대 여가에 대한 윤리적 고찰

김옥태(Ok Tae Kim) 한국체육철학회 2006 움직임의철학 : 한국체육철학회지 Vol.14 No.4