대기 중 Benzopyrene 및 중금속의 농도와 입경분포

허문영(Moon Young Heo),권창호(Chang Ho Kweon),유기선(Ki Seon Yu),최성규(Seong Kyu Choe),김경호(Kyeong Ho Kim),손동헌(Dong Hun Sohn) 대한약학회 1990 약학회지 Vol.34 No.1

Total suspended particulate (TSP) in the atmosphere was collected and size-fractionated by Andersen high volume air sampler for the past two years (Mar. 1987-Feb. 1989) in Seoul. The concentrations of several Polycyclic aromatic hydrocarbons and heavy metals were determined to investigate the atmospheric concentrations, seasonal variations and its relationship with the size distribution of suspended particulate matter. The arithmetic mean concentration of total suspended particulate was 229.48mcg/m3. The concentrations of heavy metals were 2971.94ng/m3 for Fe, 767.75ng/m3 for Zn, 765.80ng/m3 for Pb, 218.40ng/m3 for Cu, 129.91ng/m3 for Mn, respectively. And the concentration of PAHs were 3.23ng/m3 for benzo(a)pyrene, 2.71ng/m3 for benzo(k)fluoranthene, 4.53ng/m3 for benzo(ghi)perylene, respectively. The mass-size distribution of TSP was lowest in the particle size range 1.1-3.3mcm increased as the particle size increased or decreased. But PAHs, Pb and Zn abounded in particles below 2.0mcm, while Fe and Mn abouned in particles above 2.0mcm. TSP and its chemical compositions showed the seasonal variations. The concentrations of anthrophogenic origin like TSP, PAH and heavy metals in the fine particles were highest in winter and lowest in summer. PAH and Ph analyzed showed significant correlations with each other and with TSP concentration in fine particles, indicating that the particles in which they are contained have a similar behavior in the atmosphere.

Protective effects of ethanol extracts of Kalopanacis Cortex, against H₂O₂ and MMC-induced DNA damage in vitro

Young-Woo Lee(이영우),Kyoung Sub Byun(변경섭),Hyou Ju Jin(진효주),Mi Kyoung Lee(이미경),In Cheol Shin(신인철),Jin Ha Lee(이진하),Moon Young Heo(허문영),Chan Heo(허찬) 한국생물공학회 2004 한국생물공학회 학술대회 Vol.2004 No.10

대기부유분진추출물로 야기된 DNA 손상에 대한 Quercetin, Vitamin C 및 Trolox 의 보호효과

김남이,허문영,Kim, Nam-Yee,Heo, Moon-Young 대한약학회 2007 약학회지 Vol.51 No.4

In order to evaluate the genotoxicity of airborne particulate matter extracted with dichloromethane (APE), the rat microsome mediated (S-9) or DNA repair enzyme treated Comet assays were performed using the single cell gel electrophoresis in A549 human lung carcinoma cells. It was found that the cells interacting with APE showed more DNA single-strand breaks relative to untreated cells. The genotoxicity of APE was increased with the treatment of S-9 mixture. Microsome mediated DNA damage was inhibited by CYP1Al inhibitor, quercetin. The APE also showed oxidative DNA damage evaluated by endonuclease III treatment. Oxidative DNA damage of APE was inhibited by antioxidants such as vita- min C and Trolox. We also found that the vegetables or fruits extract may reduce APE-induced genotoxicity by their anti- oxidant activity and CYP1A1 inhibition.

항돌연변이 작용을 보이는 Flavonoid 인 galangin 의 마우스에서의 BP 대사의 영향

김희기,김경호,허문영,김현표 ( Hee Kee Kim,Kyeong Ho Kim,Moon Young Heo,Hyun Pyo Kim ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.2

To elucidate the antimutagenic action mechanism of flavonoids against benzo(a)pyrene induced micronucleated polychromatic erythrocytes (MNPCEs), alterations of benzo(a) pyrene metabolism were studied. Galangin, a potent antimutagen and (-)-epicatechin, a weak one, were selected and orally administered to ICR mice to compare the liver metabolic enzyme activities, arylhydrocarbon hydroxylase (AHH) and glutathione-s-transferase (GSH-T). These enzyme activities were not significantly changed between the flavonoid-treated groups and the control group. However, direct addition of galangin to the incubation mixture clearly inhibited arylhydrocarbon hydroxylase activity. These results were well correlated with the findings of the reduced metabolic conversion of [³H]-benzo(a)pyrene in vitro.

야채 및 과일추출물의 항산화작용

허찬(Chan Heo),김남이(Nam Yee Kim),김현표(Hyun Pyo Kim),허문영(Moon Young Heo) 대한약학회 2005 약학회지 Vol.49 No.3

The ethanol extracts of the mixed vegetables (Bioactive Vegetables, BV) and the mixed fruits (Bioactive Fruits, BF) were evaluated for their in vivo antioxidant activities. Four weeks treatment of oral administration was performed to mice. A KBrO3, as a potent oxidant was used to induce the oxidative stress for in vivo experiment. BV and BF were shown to possess the significant inhibitory effect of lipid peroxidation as measured by the level of malondialdehyde (MDA) formation although the potencies were not higher than those of well-known antioxidants such as vitamin C, trolox and quercetin. Furthermore, BV and BF inhibited DNA damage assessed by single cell gel electrophoresis (comet assay) and reduced the micronucleated reticulocyte (MNRET) formation of peripheral blood. Antioxidants tested also revealed potent inhibitory activities higher than BV and BF. These antigenotoxic activity profiles were similar to that of aboveentioned inhibition of lipid peroxidation. Therefore, BV and BF having mild antioxidant activity as functional food candidates may be useful natural antioxidants by the inhibiting of lipid peroxidation and the protecting oxidative DNA and chromosomal damage.

Benzo(a)pyrene 유도 DNA 손상에 대한 Genistein과 청국장추출물의 보호효과

송은정,김현표,허문영,Song, Eun-Jeong,Kim, Hyun-Pyo,Heo, Moon-Young 대한약학회 2008 약학회지 Vol.52 No.5

Chungkookjang (CKJ) is a fermented soybean product and one of favorite traditional foods in Korea. In this study, the alcoholic extract from Korean fermented soybean (CKJ) and its one of major flavonoids, genistein were evaluated for their protective effect against B(a)P induced cytotoxicity and DNA damage in HepG2 cells. CKJ extract and genistein decreased B(a)P-induced cell cytotoxicity. CKJ extract inhibited DNA single strand breaks evaluated by single cell gel electrophoresis. From RT-PCR study, it was revealed that CKJ extract decrease DNA damage induced in HepG2 cells expressing CYP1A1 and 1A2 by B(a)P. The metabolizing activities of CYP1A1 and CYP1A2, as measured by the 7-alkoxy resorufin O-deethylation (AROD) assay, showed that CKJ extract and genistein inhibited CYP1A1 and CYP1A2 activities. Genistein may contribute to these biological effects of CKJ extract at least in part. All these results indicate that CKJ extract and genistein may be useful for protection against B(a)P-induced cytotoxicity and DNA damage. Therefore, the alcoholic extract of Korean fermented soybean (CKJ) is suggested to be promising functional food which can prevent the cellular genotoxicity of dietary and lifestyle related carcinogens.

Galangin 및 양강추출물의 KBrO3 유도 DNA 및 염색체 손상에 대한 보호효과

양혜은(Hye Eun Yang),허문영(Moon Young Heo) 대한약학회 2010 약학회지 Vol.54 No.6

The aim of this study was to evaluate the in vivo effect of galangin and the 70% ethanolic extract of Alpinia officinarum (AO) toward KBrO3-induced DNA and chromosomal damage in mice. Galangin and AO inhibited the formation of 8-hydroxy-2'-deoxyguanosine (8-OH2'dG) as an indicator of DNA oxidative damage in the liver cell. Galangin and AO showed the inhibitory effect on the formation of DNA single strand break in the splenocyte by single cell gel electrophoresis (SCGE) assay and also inhibited micronucleated reticulocyte (MNRET) formation of peripheral blood in tail blood of mice. Vit-E revealed antigenotoxic effects in DNA and chromosome levels, but galangin was more potent active compound compare to vit-E under our experimental conditions. The results suggest that the extract of Alpinia officinarum containing galangin can modify the oxidative DNA and chromosomal damage and may act as chemopreventive agent against oxidative stress in vivo.

H2O2 유도 8-OH2'dG 생성 및 DNA Single Strand Break에 미치는 Galangin의 억제효과

김수희(Soo Hee Kim),허문영(Moon Young Heo) 대한약학회 2010 약학회지 Vol.54 No.1

The aim of this study was to evaluate the effect of galangin towards hydrogen peroxide-induced DNA damage. The calf thymus DNA and Chinese Hamster Lung (CHL) cells were used to measure 8-hydroxy-2'-deoxyguanosine(8-OH2'dG) as an indicator of DNA oxidative damage using high performance liquid chromatography with electrochemical detection. Hydrogen peroxide in the presence of Fe(II) ion induced the formation of 8-OH2'dG in both calf thymus DNA and CHL cells. The DNA damage effects were enhanced by increasing the concentration of Fe(II) ion and inhibited by galangin. In the single cell gel electrophoresis (Comet assay), galangin and dl-a-tocopherol showed an inhibitory effect in CHL on hydrogen peroxide induced DNA single strand breaks. Galangin showed more potent activity than dl-α-tocopherol under our experimental conditions. These results indicate that galangin can modify the action mechanisms of the oxidative DNA damage and may act as chemopreventive agents against oxidative stress.

항진균성 6-[(N-Halophenyl)amino]-7-Chloro-5 , 8-Quinolinedione 의 유전독성 평가

유충규(Chung Kyu Ryu),허문영(Moon Young Heo),윤여표(Yeo Pyo Yun) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.3

The clastogenecity and mutagenicity of antifungal 6-[(N-halophenyl)amino]-7-chloro-5,8-quinolinedione (RCK 3, 7, 13, 14, and 15) had been evaluated. Salmonella typhimurium reversion assay (Ames test) was used to test the mutagenicity of RCKs. RCK14 was mutagenic in S. typhimurium(TA98 and TA100) with and without rat liver microsomal activation. Whereas RCK3, 7, 13 and 15 were negative in Ames test with Salmonella typhimurium(TA98 and TA100). The clastogenecity was tested on the RCKs with in vivo mouse micronucleus assay. All of RCKs tested did not show any clastogenic effect in mouse peripheral blood. Thus RCKs were not supposed to cause any chromosomal damage termed micronuclei. These results indicate that RCK 3, 7, 13 and 15 have no genotoxic potential under these experimental condition.

퀘르세틴 및 퀘르세틴 배당체들의 벤조피렌에 대한 유전독성억제효과

김정한(Jeong Han Kim),허문영(Moon Young Heo) 대한약학회 1998 약학회지 Vol.42 No.4

In order to compare the suppressive effect of quercetin and its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (quercetin-3-galactoside)and rutin (quercetin-3-rhamnosyl glucoside), on the genotocicity by benzo(a)pyrene(B(a)P), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. B(a)P-induced SCEs in vitro were slightly decreased by the simultaneous treatment of quercetin and its glycosides, although there was no significant decrease. On the other hand, MNU induced micronucleated reticulocytes(MNRL7s) in vivo were significantly decreased with a dose-dependent manner in all compounds tested. However, there were no differences between quercetin aglycone and glycosides in the suppressive effects under experimental condition of this study. To elucidate, the action mechanism of quercetin aglycone and its glycosides against B(a)P-induced genotoxicity, the assay of DNA binding with B(a)P was studied. Quercetin aglycone and its glycosides inhibited B(a)P metabolism in the presence of S-9 mix and decreased the B(a)P/DNA binding in the calf thymus DNA with S-9 mix. These results suggest that antigenotoxicity of quercetin antiglycosides on B(a)P-induced genotoxicity is due to decrease of DNA binding with B(a)P through the inhibition of metabolism with B(a)P in the calf thymus DNA. Therefore, quercetin and its glycosides may act as an antigenotoxicity agent and may be useful as a chemopreventive agent of polycyclic aromaic hydrocarbons like B(a)P.