단일염기다형성 정보를 이용한 국내 홀스타인 젖소의 유효집단 크기 추정

조광현,도경탁,박경도,Cho, Kwang-Hyun,Do, Kyoung-Tag,Park, Kyung-Do 한국데이터정보과학회 2017 한국데이터정보과학회지 Vol.28 No.3

본 연구는 홀스타인 젖소, 923두에 대한 단일염기다형성 (SNP) 42,201개를 이용하여 국내 젖소집단의 유전적 특성 및 유효집단크기를 조사하고자 실시하였다. 염색체별 인접 단일염기다형성간의 평균 연관불평형 ($r^2$)은 0.22로 추정되었으며, 14번 염색체 (0.26)에서 가장 높은 반면, 27번 염색체 (0.17)에서 가장 낮게 나타났다. SNP간의 물리적 거리가 25Kb 미만인 경우에서 $r^2$은 $0.31{\pm}0.33$으로 추정되었으며, SNP간 물리적 거리가 증가할수록 $r^2$은 현저히 감소하였다. SNP간 물리적 거리가 2.5Mb 이상에서의 $r^2$은 0.04로 25Kb 미만인 경우와 비교할 때 0.27 (87.1%) 감소하였다. 국내 홀스타인 젖소의 유효집단크기는 세대수와 비례하여 감소하는 경향을 나타내었으며, 1~5세대에서 110두로 추정되었다. In this study, we investigated the genetic characteristics and the effective population size of domestic dairy cattle using 42,201 SNPs for 923 heads of Holstein cattle. The estimate for the average linkage disequilibrium ($r^2$) among the adjacent SNPs by chromosome was 0.22, and it was highest (0.26) in chromosome 14 and lowest (0.17) in chromosome 27. When the physical distance among SNPs was less than 25Kb, the estimate for the average $r^2$ was $0.31{\pm}0.33$ and it was markedly decreased as the physical distance increased. When the physical distance among SNPs was larger than 25Mb, the estimate for the average $r^2$ was 0.04, and it decreased by 0.27 (87.1%) compared with case of physical distance of less than 25Kb. There was a trend that the effective population size in Holstein dairy cattle decreased over generations and the estimate for the effective population size in the first 5 generations (1~5th generation) was 110 heads.

국내 승용마의 체형상관에 따른 품종별 비교 분석

오운용,도경탁,조병욱,박경도,김성훈,이학교,신영수,조영석,Oh, Woon-Yong,Do, Kyoung-Tag,Cho, Byung-Wook,Park, Kyung-Do,Kim, Sung-Hoon,Lee, Hak-Kyo,Shin, Young-Soo,Cho, Young-Seuk 한국데이터정보과학회 2011 한국데이터정보과학회지 Vol.22 No.3

'말산업육성법' 제정에 따라 국내 승마산업의 저변확대를 위해 자질이 우수한 국내산 승용마 생산 및 개량에 대한 연구가 절실히 필요한 실정이다. 따라서 본 논문에서는 국내에서 승용마로 활용되고 있는 3품종 (웜블러드, 더러브렛, 제주산마) 32두에 대해 12항목의 체형을 측정하여, 측정자료를 바탕으로 판별분석을 실시한 결과 81.3%가 정확하게 분류되는 것을 확인 할 수 있었다. 본 연구의 결과는 향후 체구성 분석을 통한 말의 유형 (경주마, 승용마, 재활치료마, 역용마, 비육마) 및 승용마 외모 심사를 판단하는 모형 개발에 기초자료로 활용될 것이며 이는 향후 3D 영상촬영측정치를 활용한 한국형 승용마 생산 및 개량화 연구에 활용될 것으로 사료된다. There are increasing demands for the producing and breeding new domestic riding horses for the vitalizations of horse riding industry in Korea, according as 'Horse Industry Support Act' became. In this study, we were to develop the functional relation through the conformation comparison & body composition analysis. 76 heads of 5 breeds utilized for riding horses in Korea were used and their body measurements on 12 items were measured and cluster analysis was conducted to determine the correlation relation among them. The measurements were standardized that (height, croup height, pelvis length), and (hip width, width of pelvis) were highly correlated. In these results of the decision tree, we confirmed to classify the breed type determination by their body measurements (hip height, hip width, head length, croup height). This result can be used as basic data for the development of horse type determination (racing, riding, Riding for the Disabled, Working, or fattening) through the analysis of body composition, and be utilized as the basic data for the producing and breeding new domestic riding horses through the 3D Stereosocpic image system analyze.

초보자 승마운동 시 완충고삐 사용을 통한 말의 스트레스 완화에 관한 연구

정현아 ( Hyun Ah Chung ),이경은 ( Kyung Eun Lee ),김준규 ( Joon Gyu Kim ),도경탁 ( Kyoung-tag Do ),김병선 ( Byung Sun Kim ) 한국축산학회 2022 축산기술과 산업 Vol.9 No.1

During equestrian exercise, communication between riders and horses is very important, and riders’ appropriate signals without any disturbance for the horses’ proper movements are required. Therefore, horses may get different stress depending on the rider’s riding balance, posture, and proficiency. This study was conducted to confirm the difference in the stress of horses between the beginner’s riding and the expert’s riding, additionally, to prove the effectiveness of buffer rein reducing stress even when beginners ride. Average heart rate (HR) was measured for five minutes in stages with a walk, and HR variability (HRV) were measured for three minutes in stages with both rising trot and sitting trot. HRV variables include root mean square of successive differences (RMSSD), standard deviation of normal to normal interval (SDNN), and ratio of low to high frequency components of a continuous series of heartbeats (low-frequency [LF]/high-frequency [HF]). The study involved 6 horses that underwent routine husbandry procedures. The average HR did not show a difference among groups in all exercise stages includes walk, rising trot, and sitting trot. RMSSDs showed lower values for the sitting trot when the beginner rides compared to the expert’s riding (p < 0.05), which means the stress was more increased when beginners ride. On the other hand, when beginners ride using buffer reins, the RMSSD was increased in all exercise stages compared to the case of using regular reins. SDNN was increased when the beginners ride using buffer reins in the rising trot (p < 0.05), which means the stress was more relieved. Ratio of low to high frequency components of a continuous series of heartbeats was not different among groups, but it showed decreased value when beginners use buffer reins rather than regular reins. In this study, it was confirmed that beginners induce more stress on horses than experts during riding exercise and that the use of the buffer reins by beginners more reduces the riding stress of horses compared to when they use regular reins.

Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Gene for Growth and Meat Quality Traits in the Berkshire Breed

Sang Wook Kim(김상욱),Ji Hye Jung(정지혜),Kyung Tag Do(도경탁),Kwan Suk Kim(김관석),Chang Hee Do(도창희),Jun kyu Park(박준규),Young Kuk Joo(주영국),Tae Suk Kim(김태숙),Bong Hwan Choi(최봉환),Tae Hun Kim(김태헌),Ki Duk Song(송기덕),By 한국생명과학회 2007 생명과학회지 Vol.17 No.12

4개의 후보 유전자를 분석해본 결과, 돼지의 주요 염색체 부위 및 유전자들이 주요 경제성 요인들과 관계가 있는 것으로 확인됐다. 양돈업계에서 DNA 기술을 이용한 염색체 정보를 활용하기 위해 본 연구에서는 4개의 후보 유전자에서 생성된 중합효소연쇄반응(PCR) 생성물을 비교 재 서열 함으로써 단일염기변이(SNP) 표지들을 개발했다. 또한 이들 4개의 SNP에 대해 PCR 제한효소 절편길이 다형 성(RFLP) 분석을 전개한 후, 이를 대한민국내 버크셔 종 돼지 개체군의 유전자형을 분석하는데 활용했다. 본 연구는 유용한 단일염기변이를 식별하고 돼지개체군 내 경제적으로 중요한 특성들과 SNP의 연관성을 확인하는 데 그 목적이 있다. This study was conducted to identify useful single nucleotide polymorphisms (SNPs) and determine their association with economically important traits in pig population. Four candidate gene analyses have identified important chromosomal regions and major genes associcated whit economic traits of the pig. For application of the chromosomal information to the pig industry using DNA technology, SNP markers were developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 4 candidate genes (CSF2, IL4, MYOD, RIP140). PCR restriction fragment length polymorphism (PCR-RFLP) assays were developed for these 4 SNPs and used to genotype Berkshire pig populations in Korea.

유전 및 육종 : 젖소 국제유전능력 평가를 위한 종모우별 다형질 Effective Daughter Contribution 추정

조광현 ( Kwang Hyun Cho ),최태정 ( Tae Jeong Choi ),조충일 ( Chung Il Cho ),박경도 ( Kyung Do Park ),도경탁 ( Kyoung Tag Do ),오재돈 ( Jae Don Oh ),이학교 ( Hak Kyo Lee ),공홍식 ( Hoong Sik Kong ),이준호 ( Joon Ho Lee ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.5

This study was conducted to investigate the basic concept of multiple trait effective daughter contribution(MTEDC) for dairy cattle sires and calculate effective daughter contribution(EDC) by applying a five lactation multiple trait model using milk yield test records of daughters for the Multiple-trait Across Country Evaluation(MACE). Milk yield data and pedigree information of 301,551 cows that were the progeny of 2,046 Korean and imported dairy bulls were collected from the National Agricultural Cooperative Federation and used in this study. For MTEDC approximation, the reliability of the breeding value was separated based on parents average, own yield deviation and mate adjusted progeny contribution. EDC was then calculated by lactation using these reliabilities. The average number of recorded daughters per sire by lactations were 140.57, 94.24, 55.14, 29.20 and 14.06 from the first to fifth lactation, respectively. However, the average EDC per sire by lactation using the five lactation multiple trait model was 113.49, 89.28, 73.56, 54.02 and 35.08 from the first to fifth lactation, respectively, while the decrease of EDC in late lactations was comparably lower than the average number of recorded daughters per sire. These findings indicate that the availability of daughters without late lactation records is increased by genetic correlation using the multiple trait model. Owing to the relatedness between the EDC and reliability of the estimated breeding value for sire, understanding the MTEDC algorithm and continuous monitoring of EDC is required for correct MACE application of the five lactation multiple trait model.