최근 중국 종자산업의 현황 및 발전 전망

이정로 ( Jung Ro Lee ),백형진 ( Hyung Jin Baek ),최유미 ( Yu Mi Choi ),이석영 ( Sok Young Lee ),이기안 ( Gi An Lee ),정연주 ( Yeon Ju Jung ),김정곤 ( Chung Kon Kim ),이명철 ( Myung Chul Lee ) 한국국제농업개발학회 2011 韓國國際農業開發學會誌 Vol.23 No.5

China is a one of the largest agricultural countries in the world. China consumes around 12.5 billion kilograms of seeds each year. Suchhuge demand for seeds has made the Chinese seed market more and more attractive for investment. Through analysis on the present situation and existing problems of the seed industry in China and based on the current Chinese seed industry development, some future prospects for investments are indicated. This investigation was carried out to propose the appropriate strategies on the development of the Korea seed industry as it considers its entry into the China seed market as a new growth engine in the agricultural sector. The basic law regulating the Chinese seed industry is the PRC Seed Law that generally refers to the protection of germplasm resources, verification of varieties, seed quality issues, the import and export of seeds, seed administrative management, and various rights and obligations. The regulations were aimed at the protection of the rights concerning new varieties of plants. China has two main industry associations, the National Seed Association and the China Seed Industry IP Union, that are non-profit associations consisting of entities and people engaging in the seed scientific research, production, operation and management. The China National Seed Group Co., Ltd. ("Sino Seeds") is the market leader in China regarding the seed industry. The chinese government, however, encourages investment from multinational companies as well as importation of modern crop planting management technologies and equipment. It supports the entry of investors with proven experiences in breeding and germplasm resources expansion and R&D. There has never been a better time for multinational companies with proven seed industry experience to look at building relationships with the Chinese government and enterprises.

연구보문 : 말레이시아의 약용식물 보존, 연구 및 활용현황

이석영 ( Sok Young Lee ),고호철 ( Ho Cheol Ko ),이영이 ( Young Yi Lee ),노나영 ( Na Young Ro ),성정숙 ( Jung Sook Sung ),이정로 ( Jung Ro Lee ),마경호 ( Kyung Ho Ma ),허윤찬 ( Yoon Chan Huh ),김정곤 ( Chung Kon Kim ),윤문섭 ( Mun 한국국제농업개발학회 2010 韓國國際農業開發學會誌 Vol.22 No.4

세계 12대 다양성 주요국가 중의 하나인 말레이시아는 식물의 다양성이 풍부한 나라로서 12,000여 관속 식물 중 10%인 1,200여 종이 약용식물로 분류되고 있다. 약용식물의 현지외 보존은 특성에 따라 각 기관에서 이루어지고 있으나, 말레이시아 산림연구소에서 가장 많이 보존하고 있는데 주요 약용식물 87종이 보존되고 있다. 보존 식물중에는 커리의 원료가 되는 Curcurma longa나 생강(Zingiber officinale) 등이 포함된 생강과가 10종으로 가장 많고, 그 다음이 대극과(Euphorbiaceae)로 Croton tiglium등 8종을 보존하고 있다. 보존 약용식물 중 67여 종이 이질치료에 사용되고 있으며, 37종은 열을 조절하는데, 25종은 피부병 치료에 사용되고 있는 반면 알러지, 마취제, 아메바성 이질, 항콜레라, 구토제, 괴혈병 치료에 이용되는 식물은 한 종류씩 만 보고되고 있다. 중요한 병인 류머티스 관절염에는 생강과의 Zingiber zerumbet 등 21종이 치료에 사용되고 있고, 항암에는 Leea indica 등 4종의 식물이 보고되고 있으며, 당뇨에는 Catharanthus roseus 등 6종이, 고혈압에는 Tacca integrifolia 등 2종이, 강장제로는 Zingiber ostensii 등 12종이 보고되고 있다. 현재 말레이시아에서의 약용 및 방향성 식물에 대한 연구는 약용식물의 화학성분, 생리활성 등에 강조되고 의약개발을 위한 생리활성물질연구에 중요성을 두고 있지만 실제적으로 약용식물관리의 기본이 되는 유전자원 보존, 재배, 생산물처리, 가공, 수확후 관리 기술, 표준화, 독성 및 이용성 등에 대한 연구는 많이 부족한 실정이다. Malaysia, being one of the 12 mega-diversity countries in the world, has rich plant diversity. Of the 12,000 species of vascular plants, 10% or approximately 1,200 species are reported to have medicinal properties. At the ex-situ conservation, the Forest Research Institute Malaysia(FRIM) keeps a total of 87 species of medicinal plants from the 78 genuses in 49 families. Among the 87 species of ex-situ collection at FRIM, the Zingiberaceae, which includes Cucurma longa, has the biggest collection with 10 species and the Euphorbiaceae, which includes Croton tiglium, was the second with 8 species. The other institute on ex-situ conservation is conducted in various state purposed. Among the medicinal plants conserved, a total of 67 species are used for curing dysentery, 37 species are for fever, 25 species are for skin disease while the other symptoms such as allergic, anaesthetic, anti-amoebic, anti-choleric, anti-emetic, anti-scorbutic, baldness, etc. has only one species. The majority of the current research on natural products and Medicinal & Aromatic Plants(MAP) has strengthened the basic understanding of many of the Malaysian species in terms of chemical profiles and biological activities. As well, a natural product research currently still places a lot of importance on bio-active components in relation to selected therapeutic targets and phytochemical screening. However, research is still very much lacking in MAP conservation, cultivation, product development, processing technology for raw materials, post harvest handling and technology, standardization, toxicology and efficacy.

Antioxidant Activities and Total Phenolic Contents of Three Legumes

이경준(Kyung Jun Lee),김가희(Ga-Hee Kim),이기안(Gi-An Lee),이정로(Jung-Ro Lee),조규택(Gyu-Taek Cho),마경호(Kyung-Ho Ma),이수경(Sookyeong Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6

Legumes have been important components of the human diet. They contain not only protein, starch, and dietary fiber, but also various phenolic compounds such as flavonoids and phenolic acids. The importance of phenolic compounds to human health is well known due to their antioxidant activities. In this study, three legumes (adzuki beans, common beans, and black soybeans) frequently cultivated in Korea were evaluated for their total phenolic content (TPC) and antioxidant activities using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonate)), and FRAP (ferric reducing antioxidant potential) assays. In addition, correlations between agricultural traits and antioxidant activities of these three legumes were analyzed. Antioxidant activities assessed by DPPH, ABTS, and FRAP assays and TPC showed wide variations among legumes types and accessions. Among the three legumes, adzuki beans showed higher TPC and antioxidant activity than the other two legumes. In correlation analysis, seed size showed negative correlations with antioxidant activities and TPC. In principal component analysis and hierarchical clustering analysis, each of the three legumes was clearly separate. Results of this study can be used as basic information for developing functional materials for each legume. They can also help us understand the overall antioxidant activity of the three legumes.

Comparison of Antioxidant Activities on Allium ampeloprasum Germplasm

Jung Sook Sung(성정숙),Yi Jin Jeong(정이진),Da Jeong Kim(김다정),Jung Ro Lee(이정로),On Sook Hur(허온숙),Ho Cheol Ko(고호철),Ju Hee Rhee(이주희),Hyung Jin Baek(백형진),Kyoung Yul Ryu(류경열) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.2

멜론 유전자원의 형태적 특성 및 유전적 구성 평가

이승범,장익,현도윤,이정로,김성훈,유은애,이수경,조규택,이경준,Lee, Seungbum,Jang, Ik,Hyun, Do Yoon,Lee, Jung-Ro,Kim, Seong-Hoon,Yoo, Eunae,Lee, Sookyeong,Cho, Gyu-Taek,Lee, Kyung Jun 한국작물학회 2020 한국작물학회지 Vol.65 No.4

Melon (Cucumis melo L.), one of the most important fruit crop species, is cultivated worldwide. In this study, a total of 206 melon accessions conserved at the National Agrobiodiversity Center (NAC) in RDA were characterized for nine morphological characteristics according to the NAC descriptor list. In addition, to confirm the genetic composition of each melon accession, genetic profiling was performed using 20 SSR markers. Among the 206 melon accessions, 159 (77.2%) were collected from Asia. The color of fruit flesh and skin were mostly 'white' (56.0%) and 'green' (49%), respectively. Days to female flowering (FD) and maturity (MD) of the accessions ranged from 58 to 72 and 17 to 63, respectively. The fruit length and width of the accessions ranged from 6.0 to 29.3 and 3.6 to 17.2 cm, respectively. The sugar content (SU) ranged from 2.5% to 13.2% with an average of 7.0%. In correlation analysis, SU showed positive and negative correlations with MD and FD, respectively. The accessions were classified into four clusters by cluster analysis. From the results of genetic profiling using 20 SSR markers, three accessions (K189118, K100486, and K190292) were expected to be inbred lines among 206 melon accessions. These results could expand the knowledge of the melon germplasm, providing valuable material for the development of new melon varieties to suit consumer tastes.

TiB₂ 인서트를 체결한 열간압출 금형설계 및 제작

권혁홍(Hyuk-Hong Kwon),이정로(Jung-Ro  Lee) Korean Society for Precision Engineering 2002 한국정밀공학회지 Vol.19 No.9

연구논문 : 자연과학 ; 한국과 불가리아 재래종 강낭콩 Phaseolus vulgaris L. 유전자원의 형태적 특성 및 SSR 다양성 비교

최유미 ( Yu Mi Choi ),이정로 ( Jung Ro Lee ),이기안 ( Gi An Lee ),조규택 ( Gyu Taek Cho ),정연주 ( Yeon Ju Jeong ),( T Stoilova ),박용진 ( Yong Jin Park ),이명철 ( Myung Chul Lee ),이정란 ( Jeong Ran Lee ) 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.2

본 연구는 농업유전자원센터에 보존되어있는 우리나라 및 불가리아 재래종 강낭콩 유전자원 130점을 대상으로 형태적 특성을 조사하고, 155점에 대해 분자마커를 이용하여 우리나라와 불가리아 재래종 강낭콩 유전자원의 유전적 다양성을 분석하였으며 결과는 다음과 같다. 1. 개화일수는 최소 50일에서 최대 79일, 평균 54.5일이었으며 성숙일수는 최소 25일, 최대 64일, 평균 38.9일 이었고 생육일수는 최소 83일, 최대 123일, 평균 93.4일이었다. 불가리아 재래종 자원들이 한국 재래종 자원보다 개화일수는 3.1일, 성숙일수는 3.6일, 생육일수는 6.7일 늦은 것으로 조사되었다. 2. 공시자원의 100립중은 19~71g 사이에 분포하였고, 평균 100립중은 41.2 g이었으며, 우리나라 재래종 자원은 평균 100립중이 40.9 g, 불가리아 재래종 자원은 42.4 g 이었다. 3. 조사대상 자원의 질적형질 중 생육습성은 한국 자원은 직립형이 87.6%인 반면 불가리아 자원은 덩굴성이 60.0%였으며, 한국 자원의 화색은 보라색 줄무늬가 있는 백색이 80%로 가장 많았으나 불가리아 자원은 백색자원이 52%를 차지하였다. 종피색은 한국 자원은 적색이 61%, 불가리아 자원은 백색이 48%를 차지하여 국가간 선호도와 용도에 따른 차이가 있을 것으로 보였다. 4. 10개의 SSR 마커를 이용하여 다양성을 분석한 결과 PIC 0.6181을 얻었으며, 국가별 분석 결과 다양성은 gene diversity와 PIC로 볼 때 불가리아 자원이 다양성이 높은 것으로 조사되어 불가리아에서 도입한 강낭콩 유전자원이 강낭콩 수집단의 유전적 다양성 증대에 기여한 것으로 평가된다. The morphological traits and the genetic diversity of 130 common bean landraces from Korea and Bugaria were evaluated. Days from sowing to flowering were ranged from 50 to 79 days with an average of 54.5 days. Days from flowering to maturing were ranged from 25 to 64 days with an average of 38.9 days, and days from sowing to maturing were ranged from 83 to 123 days with an average of 93.4 days. Common beans originated from Korea tend to bloom and mature earlier than those from Bulgaria. In growth habit, 87.6% of being originated from Korea are erect type, but 60.0% from Bulgaria are climbing type. Also qualiitative characteristics of both countries were different from each other. For example, 80% of the colors of flower were white with purple stripe in Korea, but 52% of those were white in Bulgaria. One hundred fifty-five common beans from Korea and Bulgaria were analyzed using 10 SSR markers. Ninety-seven alleles were detected with a lowest five at the BM161, BM181, and BM211 and a highest 18 at the BM156. The average polymorphism information content (PIC) was 0.61. Genetic diversity was higher in the Bulgarian population than that of Korea.

Efficient Cryopreservation of In Vitro Grown Shoot Tips of Strawberry (Fragaria x ananassa Duch.) Germplasm Using Droplet-Vitrification

배진주(Jinjoo Bae),이선이(Sun-Yi Lee),송재영(Jae-Young Song),이정로(Jung-Ro Lee),윤문섭(Munsup Yoon),이정윤(Jung-Yoon Yi),김행훈(Haeng-Hoon Kim),이영이(Young-Yi Lee) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6

Cryopreservation method using a droplet vitrification was applied to the thirty-one strawberry accessions of in vitro grown shoot tips. A protocol with 0.3 - 0.5 M preculture followed by C4 loading and B1 dehydration solutions efficiently implemented cryopreservation of twenty-six strawberry accessions. The highest regrowth rate was 85.8% for PHS0007 and others were ranged between 85.8% and 21.0%. A slightly modified protocol was applied to five accessions. With these two protocols, twenty-eight accessions obtained more than 40% regrowth rate. This study showed that the droplet vitrification method was able to practically implement cryopreservation of in vitro grown shoot tips of broad range of strawberry germplasm (105).

기원이 다른 고수 유전자원의 폴리페놀 함량 및 항산화 활성

정해곤(Hae-Gon Chung),이영민(Young-Min Lee),권택윤(Taek-Ryoun Kwon),이주희(Ju-Hee Rhee),강만정(Man-Jung Kang),이정로(Jung-Ro Lee),고호철(Ho-Cheol Ko),곽재균(Jae-Gyun Gwag),김연규(Yeon-Gyu Kim) 한국약용작물학회 2013 한국약용작물학회 학술대회논문집 Vol.2013 No.2

Gas chromatography를 이용한 향미 유전자원의 2-acetyl-1-pyrroline 정량분석

김정순(Jeong-Soon Kim),박원성(One-Sung Park),안상낙(Sang-Nag Ahn),이정로(Jung-Ro Lee),곽재균(Jae-Gyun Gwag),김태산(Tae-San Kim),이석영(Sok-Young Lee) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.5

본 실험은 국내 육성향미자원과 유전적 배경이 서로 다른 도입향미 유전자원의 2AP 함량을 비교분석하여, 2AP 함량이 높고 농업적 형질이 우수한 자원을 선발하여 우수한 향미자원의 육성에 필요한 기초 자료를 제공하고자 실시하였다. 3종류의 유기용매에 대한 추출효율은 ethanol>acetonitle>methanol 순으로 확인되었으며, ethnanol의 경우 30-90℃의 온도범위에서 3가지 유기용매 중 2AP 추출효과가 가장 뛰어났고, 특히 90℃에서 30분간 추출하였을 때 2AP 추출함량이 가장 높았고 추출 시간이 경과할수록 2AP의 함량이 낮았다. 현미의 분쇄정도에 따른 2AP 추출함량은 5초간 분쇄하였을 경우 가장 높았으며 분쇄 시간이 길어질수록 낮아졌다. 선발한 30품종 중 국내 향미자원과 자포니카형 도입자원의 평균 출수일은 각각 130.3일과 131.8일로 비슷하였으며 인디카형 도입자원은 약 137.1일로 가장 늦었다. 평균 1,000립중은 인디카형 도입자원이 19.9 g으로 가장 낮았으며, 국내 향미자원이 21.65 g이었고 자포니카형 도입자원은 25.5 g으로 높았다. 선발자원의 향에 대한 관능검사는 국내 향미자원 5품종 중향남벼 2호, 아랑향찰벼가 중간 정도의 향을 띄었고 인디카형 도입자원 19품종 중에서 11품종(57.9%)과 자포니카형 도입자원 6품종 중 2품종(33.3%)이 중간 이상의 향을 나타났다. 선발한 30품종의 평균 2AP 함량은 국내 육성 향미자원이 도입 향미자원에 비해 낮았으며 도입자원에서는 인디카형이 자포니카형보다 높은 2AP 함량을 나타내었다. 국내 육성 향미자원은 아랑향찰벼 외 4품종과 도입 향미자원 중 인디카형은 Basmati6313 외 18품종 및 자포니카형은 자향나 861 외 5품종을 선발하였다. This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to 90℃. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at 90℃ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.