겔형태 인공누액이 각막실질세포 및 결막상피세포에 미치는 영향

이종수,김은희,김수진,이지은,김나미,Jong Soo Lee,Eun Hee Kim,Soo Jin Kim,Ji Eun Lee,Na Mi Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the biological effects and cytotoxicity of gel-type artificial tears on human corneal keratocytes and conjunctival cells in vitro. Methods: Human corneal keratocytes and conjunctival epithelial cells were exposed to Soothe® and Systane® at variable concentrations. Evaluations were conducted through an MTT-based calorimetric assay to measure the metabolic activity and through a lactate dehydrogenase (LDH) assay to assess cellular damage. Apoptotic response was examined using fluorescent microscopy and flow cytometric analysis, and cellular morphologic results were evaluated with a transmission electron microscope. Results: The inhibitory effects of corneal keratocyte and conjunctival cell proliferations increased at higher concentrations and longer exposure times to Soothe® and Systane®. The LDH titers increased after Soothe® exposure, but showed no significant difference after Systane® exposure. Soothe® and Systane® treatments both produced fluorescence, representing apoptotic cells. In flow cytometry, the maximal apoptotic response was observed for both types of artificial tears, although Systane® showed less edema, as well as reduced cytoplasmic and nuclear cell degeneration compared to those of Soothe®. Conclusions: The apoptotic responses of Soothe® and Systane® are associated with inhibitory effects of human corneal keratocyte and conjunctival epithelial cell proliferations. To inhibit the cellular proliferation of human corneal keratocytes and conjunctival epithelial cells, Systane® may be less severe than Soothe® at higher concentrations and longer exposure times. J Korean Ophthalmol Soc 2010;51(12):1643-1651

익상편조직의 섬유모세포에 미치는 사이클로스포린의 영향

이종수,이승욱,이상준,김나미,Jong Soo Lee,Seung Wook Lee,Sang Jun Lee,Na Mi Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. Methods: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 μg/ml (0.0001%), 1 μg/ml (0.0001%), 10 μg/ml (0.001%), 100 μg/ml (0.01%), or 500 μg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-α and IL-1b. IL-6, IL-8 was performed using ELISA kits. Results: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-α and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). Conclusions: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-α were observed compared with that of the control. J Korean Ophthalmol Soc 2012;53(3):466-472

정상 및 원추각막에서 각막 전, 후면의 융기 및 시상면만곡도의 비교분석

이종수,이승욱,이승민,이미현,Jong Soo Lee,Seung Wook Lee,Seung Min Lee,Mi Hyun Lee 대한안과학회 2012 대한안과학회지 Vol.53 No.10

Purpose: To compare changes of anterior and posterior corneal elevation, and sagittal curvature according to the severity of keratoconus and to compare differences between keratoconus and normal cornea. Methods: A total of 81 eyes diagnosed with keratoconus and 20 eyes of normal subjects were evaluated with a Pentacam Scheimpflug camera. The keratoconus eyes were divided into 3 groups according to mean keratometer (K): mild (K ≤ 47.0 diopters (D)), moderate (47.0 to 52.0 D), and severe (52.0 D≥). The following parameters were obtained to evaluate the correlation of keratoconus: corneal thickness, anterior and posterior corneal elevation, and sagittal curvature. Results: Out of 81 keratoconus eyes, 56 eyes were mild, 12 eyes were moderate, and 13 eyes were severe keratoconus. The mean central corneal keratometer, anterior and posterior corneal elevation, and sagittal curvature of the keratoconus eyes were 49.7 D, 22.07 μm, 38.16 μm, 52.76 D and the values increased statistically compared to the normal eyes. Furthermore, the values increased significantly with the severity of keratoconus. ROC curve analysis showed the estimated meaningful value for anterior and posterior corneal elevation and sagittal curvature of keratoconus for diagnosis; there were no diagnostic values for corneal thickness and refractive power. Conclusions: The index of 5.5 μm for mean anterior elevation, 12.5 μm for mean posterior elevation, and 44.5 μm for mean sagittal curvature using the PentacamⓇ are useful to diagnose keratoconus. Variation of anterior and posterior elevation, and sagittal curvature measured by PentacamⓇ are useful in understanding the process of keratoconus. J Korean Ophthalmol Soc 2012;53(10):1432-1437

몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성

이종수,오준세,김나미,금종화,이석건,Lee, Jong-Soo,Oh, Jun-Sei,Kim, Na-Mi,Keum, Jong-Hwa,Lee, Suk-Kun 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

현재 식품첨가물로 많이 사용되고 있는 유기산과 안정제 및 색소 등의 각종 세균과 Sacch. cerevisiae 에 대한 향균 특성을 조사하였다. 산도 조절용으로 사용되고 있는 젖산, 사과산, 호박산 및 주석산은 L. acidophilus와 Sacch. cerevisiae에 대하여 향균성이 없었으나 B. subtilis등의 세균에 대하여는 향균성이 있었고 특히 사과산은 P. aeruginosa에 대하여 강한 향균력이 있었다.(최소생육저지농도 : 0.05%). 안정제로서의 알긴산과 펙틴은 B. subtilis, E. coli, P. aeruginosa에 대하여 비교적 강한 향균성을 보였고 L. acidophilus에 대하여는 향균성이 없었다. 황색 색소(홍화엘로우)와 적색 색소(Red powder-N)는 향균성이 없었고 표백제인 $NaHSO_3$의 세균에 대한 최소생육저지농도는 0.05%, Sacch. cerevisiae에 대하여는 0.5%로 향균성이 있었다. In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

자동차 차체재료용 Al-Mg-Si-(Be) 합금의 도장소부경화성 개선에 관한 연구

이종수,김동건,김석원,우기도 ( Jong Soo Lee,Dong Keon Kim,Sug Won Kim,Kee Do Woo ) 한국주조공학회 1998 한국주조공학회지 Vol.18 No.2

N/A Al-Mg-Si system alloys are capable of providing a good combination of formability and paint bake hardening response. For this reason, they have been proved suitable for automotive panel parts. The pre-aging treatment for prohibition of G.P. I zone formation is needed to increase the bake hardening of the Al-Mg-Si alloys. This is because G.P.I zone formed by natural aging decreases the effect of bake hardening. Be elements are known to increase the strength and to improve the bake hardening in Al-Mg-Si system alloys, respectively. Therefore the strength and bake hardening will be affected by the addition of Be in Al-Mg-Si alloy. In this paper, the effects of Be addition, two-step aging, and retrogression and re-aging (RRA) treatment on the bake hardening in Al-Mg-Si alloys will be investigated by hardness, tensile test, and differential scanning calorimetry. The rate of β` formation in the Al-Mg-Si alloy was significantly accelerated by the small addition of Be. By the. addition of Be in Al-Mg-Si alloy, G.P.I zone easily decomposes during retrogression treatment at 225˚C for 3 minutes. The hardness of Al-Mg-Si-(Be) alloy were increased by RRA (150˚C/20 min → 225/3 min → 180˚C/30 min) treatment. (Received February 17, 1998)

잉어 내장중의 Mouse 치사성분 Cyprinol Sulfate의 분리 및 생리 활성

이종수,Lee, Jong-Soo 생화학분자생물학회 1994 한국생화학회지 Vol.27 No.5

양식산 잉어(Cyprinidae, Cyprinus carpio) 내장의 MeOH 추출물로부터 mouse의 치사성분을 분리 정제하였다. 이 성분은 HRFABMS로부터 분자식은 $C_{27}H_{48}O_{8}S$로 판명되었으며 NMR 등 각종 기기 분석 자료를 검토한 결과, 잉어과 어류의 담낭 중에 존재한다고 알려진 독특한 성분인 cyprinol sulfate($5{\alpha}-cholestane$ $3{\alpha}$, $7{\alpha}$, $12{\alpha}$, 26, 27-pentol, 26-sulfate)로 동정되었다. Cyprinol sulfate의 mouse치사량은 2.0~3.0mg/MU 이었고, 약한 용혈성($500{\mu}g/ml$)과 prolyl endopeptidase 활성 저해작용($IC_{50}$ > $200{\mu}g/ml$)을 나타내었으나 Bacillus subtilis와 Aspergillus niger에 대한 항균성이나 L1210 세포에 대한 독성은 나타내지 않았다. A toxic component to mice was isolated from the viscera of cultivated carp (Cyprinus carpio) as white powder. Molecular formula was obtained to be $C_{27}H_{48}O_{8}S$ on the basis of the HRFABMS data. NMR spectra of the isolated compound were corresponded with those of $5{\alpha}-cyprinol$ except the $C_{26}$ sulfated carbon. Therefore, it was identified as cyprinol sulfate ($5{\alpha}-cholestane$ $3{\alpha}$, $7{\alpha}$, $12{\alpha}$,26,27-pentol 26-sulfate). It showed toxicity to mice (2~3 mg/MU, i.p.), hemolytic activity ($500{\mu}g/ml$) and prolyl endopeptidase inhibition ($IC_{50}$ > $200{\mu}g/ml$), but, had no antibacterial, antifungal and cytotoxic activity to L1210 cells.

전침이 중풍재활에 미치는 영향에 대한 문헌적 고찰

이종수,심우진,Lee, Jong-Soo,Sim, Woo-Jin 척추신경추나의학회 2002 대한추나의학회지 Vol.3 No.1

Objectives : Electroacupuncture(EA) has been suggested as a treatment for stroke rehabilitation. But whether, how much, by what mechanism and when it is effective has not been answered satisfactorily. Therefore it is important to critically review clinical trials and laboratory researches about EA for stroke rehabilitation. Subjectives : We researched various recent sources of EA for stroke rehabilitation such as medical journals and especially tried to review methodologically best randomized controlled trials(RCTs). Results and Conclusions : 1) EA increases brain plasticity, activity, blood flow and secretion of neuropeptides in CNS. 2) EA is significantly effective at the case that more than half of the neural motor pathway is reserved. 3) The acupoints, frequncy and intensity of EA should be determined by patient-specific symptoms of stroke. 4) More studies is needed for merdian functions for stroke rehabilitation.

실사적인 3차원 물체 모델링 기법 개선

이종수,Lee, Jong-Soo 한국정보처리학회 2003 정보처리학회논문지B Vol.10 No.2

본 논문에서는 실사적인 3D 물체 모델링을 위해 제안된 기법들을 고찰하고, 보다 정확한 모델링을 위한 방안들을 제시하고 있다. F-행렬 추정기법의 개선과 스테레오 영상 평행화기법을 적용함으로 물체 모델링의 정확도를 높히는데 필수적임을 보여준다. In this paper, in-depth considerations are given to various techniques suggested for photographic 3D modelling and possible enhancements of the techniques are discussed. It is found that both the improvement of fundamental matrix estimation techniques and the stereo image rectification processing stage are necessary for more accurate 3D modelling.

β-galactosidase를 생성하는 고온성(高溫性) 세균(細菌)의 생리적(生理的) 특성(特性)에 관한 연구(硏究)

이종수,김찬조,Lee, Jong Soo,Kim, Chan Jo 충남대학교 농업과학연구소 1982 Korean Journal of Agricultural Science Vol.9 No.1

고온균(高溫菌)의 생리적(生理的) 특성(特性)과 내열기구(耐熱機構)에 관한 기초자료(基礎資料)를 얻고자 온천토양(溫泉土壤)에서 ${\beta}$-galactosidase를 생산(生産)하는 고온균(高溫菌)을 분리(分離)하여 동정(同定)하고, 몇가지 주요(主要)한 생리적(生理的) 특성(特性)을 검토(檢討)하였으며, 그의 균체지방산(菌體脂肪酸) 조성(組成)을 gas chromatography로 분석(分析)하여 다음과 같은 결과(結果)를 얻었다. 1. ${\beta}$-galactosidase를 생산(生産)하는 공시(供試) 균주(菌株)는 Thermus속(屬)으로 동정(同定)되었다. 2. 분리선정(分離選定)한 균주(菌株)의 최적(最適) 생육온도(生育溫度)는 $65^{\circ}C$이었고, $37^{\circ}C$에서 생육(生育)하지 않는 절대(絶對) 고온균(高溫菌)이었으며 최적(最適) pH는 6.5내외(內外)이었다. 3. NaCl에 대(對)한 내성(耐性)은 NaCl 1% 이상(以上)에서 생육(生育)하지 못했다. 4. 공시균주(供試菌株)의 항생물질(抗生物質) 내성(耐性)은 Penicillin G는 $10{\mu}g/m{\ell}$, Chloramphenicol은 $0.5{\mu}g/m{\ell}$이었다. 5. Vitamin 요구성(要求性)은 Ca-pantothenate와 pyridoxine-HCl을 절대적(絶對的) 생육인자(生育因子)로 요구(要求)하였고 niacin을 자극적(刺戟的) 생육인자(生育因子)로 요구(要求)하였다. 6. 공시균주(供試菌株)는 최대(最大) 흡수파장(吸收波長)이 420nm 부근(附近)의 색소(色素)를 생성(生成)하였고 그의 생성능(生成能)은 약(弱)하였다. 7. 공시균주(供試菌株)의 균체 지방산(脂肪酸) 조성(組成)은 palmitic acid 60.20%, lauric acid 11.80%, myristic acid 7.56%, behenic acid 4.25%, capric acid 1.77%, stearic acid 2.13%, arachidic acid 1.53%이었다. This experiment was carried out to elucidate the thermotolerant properties of a thermophilic bacterium which was isolated from soils of the hot springs area and selected for the ${\beta}$-galactosidase production. This bacterium was identified as a strain belong to the genus Thermus. Biochemical and physiological characteristics of this strain were studied, including the investigation of the fatty acid composition of its neutral fats. The results obtained were summarized as follows. 1. Optimal temperature and pH for growth of this strain were $65^{\circ}C$ and pH 6.5 respectively, and it was found to be an absolute thermophilic bacterium which could not grow at the temperature below $43^{\circ}C$. 2. No growth was obtained in the medium which contained more than 1.0% of sodium chloride. 3. The tolerable concentration of antibiotics were 10mg of penicillin G per ml of medium and 0.5mg of chloramphenicol per ml respectively. 4. This strain had auxotrophilic requirements for calcium-pantothenate and pyridoxin-HCl as an essential factor and for niacin as a stimulative factor. 5. Yellow pigment was released into the liquid culture of this strain, which showed maximum absorption at 420 nm. 6. Fatty acid composition of neutral fats of the strain was palmitic acid, 60.20%; lauric acid, 11.80%; myristic acid, 7.56%; behenic acid, 4.25%; capric acid, 1.77%; stearic acid, 2.13%; arachidic acid, 1.53%; and others unidentified, 10.7%.

Candida sp. L-16이 생성하는 Xylitol dehydrogenase의 생성조건에 관한 연구

이종수 대구산업정보대학 1995 논문집 Vol.9 No.2

The opitmal cultural conditions such as carbon and nitrogen sources, cultural temperature, initial pH, aeration and cultural time for production xylitol dehydrogenase which catalyzed the conversion of xylitol to xylulose from Candida sp. L-16. were investigated. The maximum yield of xylitol dehydrogenase was observed with cultivation in a medium containing 5.0% D-xylose as a carbon source and 0.3% yeast extract, 0.3% malt extract and 0.5% bactopeptone as nitrogen sources and pH 4.5. The optimal conditions for production of xylitol dehydrogenase were 28℃ of cultural temperature and 4.5 of initial pH. It was found that the production of enzyme reached a maximum in a 3 days of cultivation in the 100㎖Erlenmeyer flask containing 40㎖ broth.