Polyacetylene 에 의한 Benzo ( a ) pyrene 대사의 변화 및 total DNA - Benzo ( a ) pyrene 대사물질 결합의 감소

박진규,김신일,이천배 ( Jin Kyu Park,Shin Il Kim,Chun Bae Lee ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2

Activities of benzo(a)pyrene(BP) metabolizing enzymes and amounts of BP-metabolites were determined in hephatic microsomes of mice treated with two polyacetylene compounds, panaxynol, panaxydol which purified from Korean ginseng root(panax ginseng C.A. Meyer) in order to investigate the effect of polyacetylene compounds on BP-metabolism. As results, two polyacetylenes induced epoxide hydratase(EH) markedly as compared with arylhydrocarbon hydroxylase(AHH). Among BP-metabolites, BP-7,8-diols and BP-quinone compounds increased in hephatic microsome while BP-9,10-diols rather reduced by polyacetylene treatment. In connection with these results, the increment of water soluble BP-metabolites in rat urine and inhibition of total covalent binding between BP-metabolites and DNA in vitro suggest that polyacetylene compounds have a potential to reduce BP induced carcinogenesis.

Isolation and Characterization of an Extracellular p-Nitrophenylacetate Hydrolase from Pine

박진규,김유삼,Park, Jin-Kyu,Kim, Yu-Sam 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.3

p-Nitrophenylacetate hydrolase was detected in the extracellular fluid of pine pollen and purified by the combination of DEAE cellulose ion exchange chromatography and Sephadex G-100 gel filtration. The purification steps resulted in about 59 fold purification with 37% recovery of the enzyme activity. The purified enzyme showed a single protein band on the acrylamide gel after electrophoresis and the molecular weight of the enzyme was estimated to be 47,000 dalton by gel filtration with a calibrated Sephadex G-100 column. Its enzyme activity was inhibited by a thiol-directed reagent, p-chloromercuribenzene sulfonic acid. The optimum pH of this enzyme was 8.0 and a typical Michelis-Menten type saturation pattern with increasing concentration of p-Nitrophenylacetate was obtained. From the double reciprocal plot its Km value was calculated to be $166\;{\mu}M$. This enzyme also hydrolyzed p-nitrophenylacetate but not tripalmitin. 송화가루의 추출물로부터 p-nitrophenylacetate를 분해 하는 효소(PNA hydrolase)를 검정하고 이 효소를 DEAE cellulose ion exchange chromatography와 Sephadex G-100 gel filtration을 통하여 정제하였다. 정제효소의 회수율은 37.1%로서 정제도는 약 59배였으며 polyacrylamide gel electrophoresis에 의하여 단일 단백질띠를 보이는 순도를 나타냈다. Sephadex G-100 gel filtration에 의하여 측청된 분자의 크기는 약 47,000 dalton 이었고 p-chloromercuribenzene sulfonic acid 에 의해서 효소의 활성이 저해되었다. 효소의 최적 pH 는 8.0 정도였고 기질인 p-Nitrophenylacetate에 대한 반응은 Michaelis-Menten 이론에 부합되었으며 Km은 $166\;{\mu}M$ 이었다. 이 효소는 또한 p-nitrophenylpalmitate를 가수분해 하였으나 tripalmitin에는 거의 작용하지 않았다.

동일 채널 간섭 환경에서 OFDM 전송 방식을 위한 송신 전력 제어

박진규,임창헌,Park, Jin-Kyu,Lim, Chang-Heon 한국통신학회 2007 韓國通信學會論文誌 Vol.32 No.3A

본 논문에서는 동일 채널 간섭이 존재하는 셀룰러 환경에서 OFDM 통신 시스템들이 각기 목표로 하는 전송속도를 달성하기 위한 송신 전력 조절 방안을 제시하였다. 일반적으로 다중 반송파 시스템에 대한 최적 또는 준 최적 송신 전력 할당 방안은 주파수 대역별로 채널 상태에 따라 송신 전력을 조절하는 형태인 것으로 알려져 있으며, 이는 WF(waterfilling)이나 IWF(iterative waterfilling) 형태로 발표된 바 있다. 하지만 반송파별로 송신 전력을 달리하는 방식을 구현하기 위해서는 각 대역별 채널 상태와 관련된 정보를 별도의 궤환 링크(feedback link)를 통해 수신기에서 송신기로 전송해야 한다. 만약 채널 상태가 빠르게 변하거나 반송파의 개수가 크다면 이로 인한 부담이 상당할 수가 있다. 이에 본 논문에서는 이런 부담을 줄이기 위한 방안으로 부반송파마다 동일한 송신 전력을 사용하는 방식에 대한 전력 조절 알고리듬을 제시한다. 그리고 제안한 알고리듬이 수렴한다는 것을 증명하고, 기존의 IWF 방식과 복잡도를 비교하며, 컴퓨터 실험을 통해 수렴 특성을 살펴보고자 한다. This paper presents a power control scheme for OFDM based wireless communication systems in a multicell environment with co-channel interference which enables each system to achieve its target level of transmission bit rate. Generally, the optimal or near optimal power control scheme for multicarrier systems is Down to control the power level of each subcarrier in accordance with the associated channel status, which may be found in WF(waterfilling) and WF(iterative waterfilling) schemes. However, this requires the channel state information associated with every subchannel to be fed back from the receiver to its transmitter for successful power control. If the wireless channel exhibits relatively fast fading or the number of subcarriers is large, this may result in a considerable overhead. Here, in order to alleviate this problem, we propose a power control strategy for an OFDM systems maintaining the same power level over all the subcarriers. Also we prove its convergence, compare its complexity with that of the existing IWF algorithm, and examine its convergence characteristic through computer simulations.

쥐에서 Benzo ( a ) Pyrene 의 담즙 및 뇨배설에 미치는 인삼의 영향

박진규,고지훈 ( Jin Kyu Park,Ji Hun Ko ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

The biliary and urinary excretion of benzo(a)pyrene(BP) were investigated in the rat with a view to determining the effect of Panax ginseng C.A. Meyer. After pretreatment of rats with crude total water extract (TWE) of ginseng (250 ㎎/㎏ b.w., p.o) for 3 consecutive days, [³H]BP (2 mCi/mmol) was injected into i.v. at the 1st. day after the last treatment. The biliary excretion of BP in ginseng treated group was reduced to 71% of the values of the control without any specific change of bile flow, while the urinary excretion of BP was increased by about 25% than that of the control value within the period of observation. Hence, under the conditions of the present study, these results suggest that the oxidative metabolism which is rate limiting in the excretion of BP was depressed by ginseng treatment and the proximate mutagenic metabolites of BP which undergoes an entero hephatic circulation (EHC) might also be eventually cleared by the urinary excretion although the major route of excretion of BP and its metabolites via bile.

Effect of Panak Ginseng on Biliary and Urinary Excretion of Benzo(a)Pyrene in Rat

박진규,고지훈,Park, Jin-Kyu,Ko, Ji-Hun Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.4

쥐에서 Benzo(a)pyrene(BP)의 담즙 및 뇨배설에 미치는 인삼의 영향을 관찰하였다. 인삼의 물추출물(total water extract, TWE, 2550 mg/kg b.w.)을 3일간 경구투여 후 $[^3H]$BP(2mCi/ mmol)을 최종 투여한 다음날 꼬리정맥 주사하여 담즙으로 배설되는 방사능 표지 화합물을 조사한 결과 인삼 TWE 처리군에서는 담즙을 통해 배설되는 BP의 양이 대조군보다 29% 정도 담즙산 유출량의 변동없이 감소함을 확인하였다. 반면에 뇨로 배설되는 BP의 수용성 대사물질들의 양은 관찰기간내에 대조군보다 약 25% 증가하였다. 이와 같은 결과는 BP의 배설에 있어서 BP 대사의 율속단계인 산화단계가 인삼투여로 억제되어 장간순환(entero hepahatic circulation, EHC)되는 BP의 반응성 돌연변이 물질들을 궁극적으로 뇨를 통해 더 배설시킬 수 있다는 것을 시사한다. The biliary and urinary excretion of benzo(a)pyrene(BP) were investigated in the rat with a view to determining the effect of Panax ginseng C.A. Meyer. After pretreatment of rats with crude total water extract (TWE) of ginseng (250 mg/kg b.w., p.o) for 3 consecutive days, $[^3H]$BP (2mci/mmol) was injected into i.v. at the 1st. day after the last treatment. The biliary excretion of BP in ginseng treated group was reduced to 71% of the values of the control without any specific change of bile flow, while the urinary excretion of BP was increased by about 25% than that of the control value within the period of observation. Hence, under the conditions of the present study, these results suggest that the oxidative metabolism which is rate limiting in the excretion of BP was depressed by ginseng treatment and the proximate mutagenic metabolites of BP which undergoes an entero hephatic circulation (EHC) might also be eventually cleared by the urinary excretion although the major route of excretion of BP and its metabolites via bile.

적응변조와 안테나 교환 다이버시티 기술을 사용한 대역 확산 시스템

박진규,임창헌,Park, Jin-Kyu,Lim, Chang-Heon 한국전자파학회 2007 한국전자파학회논문지 Vol.18 No.4

교환 다이버시티 방식은 선택 다이버시티 방식보다 성능은 다소 떨어지지만, 안테나 개수와 상관없이 1개의 수신 RF 회로만 사용한다는 장점 때문에 널리 사용되고 있는 기술이다. 본 논문에서는 적응 변조 기능을 갖는 대역 확산 시스템에 안테나 교환 다이버시티(switched diversity) 기술을 적용한 방식을 제안하고, 해당 방식의 평균 비트 전송 속도를 최대로 하는 안테나 교환 임계값을 유도하였다. 그리고 안테나 다이버시티를 사용하지 않는 방식과 선택 다이버시티를 사용하는 방식을 비교 대상으로 하여 제안한 시스템의 성능을 심볼 당 평균 비트 수와 송신 중단 확률 그리고 평균 BER 측면에서 비교하였다. 성능을 비교한 결과, 제안한 방식과 선택 다이버시티를 사용하는 방식은 채널 경로 개수가 증가함에 따라 심볼 당 평균 비트 개수 측면에서 그 격차가 줄어든다는 사실을 확인할 수 있었다. 그리고 평균 SNR이 높을 때는 제안한 방식이 갖는 송신 중단 확률이 선택 다이버시티를 사용한 경우보다 다소 높게 나타나지만, 평균 SNR이 낮을 때는 거의 대등한 송신 중단 확률을 갖는다는 것을 알 수 있었다. The switched diversity, although its performance is a little inferior to the selection diversity, is widely used due to its advantage that only one RF circuit is required for its operation without respect to the number of antennas in use. In this paper, we propose an application of the antenna switched diversity to a spread spectrum system with adaptive modulation and derived the optimal antenna switching threshold to maximize the average transmission bit rate. We also compared the performances of the proposed system with those of the two cases using a single antenna and the selection diversity with two antennas in terms of the average number of bits per symbol(BPS), the probability of no transmission, and the average BER. The performance analysis shows that, if the number of paths in a multipath channel environment increases, the performance of the proposed scheme becomes closer to that of the selection diversity based system in terms of the average BPS. Furthermore, the proposed scheme produces as almost high the probability of no transmission as the selection diversity based system for the case of low average SNR, although the former yields a little higher probability of no transmission than the latter fer the case of high average SNR.

Polyacetylene Induced Alterations in Benzo(a)-pyrene Metabolism and Reduction of Total DNABP metabolites Binding

박진규,김신일,이천배,Park, Jin-Kyu,Kim, Shin-Il,Lee, Chun-Bae 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2

인삼(Panax ginseng C.A. Meyer)의 석유에텔 분획으로부터 순수하게 분리한 polyacetylene 화합 물들(panaxynol, panaxydol)을 투여한 하우스 (ICR, C57BL/6 strain)의 간 마이크로좀에서의 AHH, EH, 그리고 GSH-T의 활성도 변화 및 in vitro에서 BP 대사물질들의 생성량을 관찰하였다. 그 결과, AHH에 비해 EH 활성도의 증가가 현저하게 나타났으며 생성된 BP 대사물질들 중 특히 BP-7, 8-diol과 BP-quinone 화합물들의 양이 대조군보다 뚜렷이 증가한 반면에 BP-9, 10-diol의 경우는 대조군보다 양이 감소되었다. 이와 관련해서 polyacetylene을 투여한 쥐 (Wistar rat)에서의 urinary excretion을 통한 BP 수용성 대사물질들의 양적인 증가, 그리고 DNA와 BP 대사물질들간의 in vitro 공유결합 생성의 뚜렷한 감소 등은 이들 polyacetylene 화합물들이 BP에 의해 유도되는 발암을 억제시켜 줄 것을 시사한다. Activities of benzo(a)pyrene(BP) metabolizing enzymes and amounts of BP-metabolites were determined in hephatic microsomes of mice treated with two polyacetylene compounds, panaxynol, panaxydol which purified from Korean ginseng root(panax ginseng C,A. Meyer) in order to investigate the effect of polyacetylene compounds on BP-metabolism. As results, two polyacetylenes induced epoxide hydratase(EH) markedly as compared with arylhydrocarbon hydroxylase(AHH), Among BP-metabolites, BP-7,8-diols and Bp-quinone compounds increased in hephatic microsome while BP-9,10-diols rather reduced by polyacetylene treatment. In connection with these results, the increment of water soluble BP-metabolites in rat urine and inhibition of total covalent binding between BP-metabolites and DNA in vitro suggest that polyacetylene compounds have a potential to reduce BP induced carcinogenesis.

담관암세포주에서 지유(地楡) 에탄올 추출물의 항암 효과

박진규,김영철,Park, Jin-Kyu,Kim, Young-Chul 대한한방내과학회 2012 大韓韓方內科學會誌 Vol.33 No.4

Objectives : This study was performed to investigate the antineoplastic effect of ethanol extracts from Sanguisorbae Radix on cholangiocarcinoma cells that was established from biliary tract cancer tissue. Materials and Methods : Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were studied. The mRNA expression of Caspase 3, 8, 9, Bcl-2, Bax, P53, and P21 was examined by RT-PCR. Cell viability was determined by MTT assay. The cell cycle was analyzed by flow cytometry and apoptosis by cell death detection ELISA kit. Results : Proliferation of SNU-1079 and SNU-1196 was inhibited by Sanguisorbae Radix treatment in a dose-dependent manner. All cells treated with Sanguisorbae Radix showed increased dose- and time-dependent apoptosis. The expression of caspase 3, 8, 9, p53, and p21 was increased in all cells after the treatment of Sanguisorbae Radix. The expression of Bcl-2 was decreased in SNU-1196 and Bax expression was increased in all cells after the treatment of Sanguisorbae Radix. Conclusions : These results suggest that Sanguisorbae Radix would be beneficial in the treatment of cholangiocarcinoma.

송화가루로부터 p - Nitrophenylacetate Hydrolase 의 정제 및 효소의 특성

박진규,김유삼 ( Jin Kyu Park,Yu Sam Kim ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.3

p-Nitrophenylacetate hydrolase was detected in the extracellular fluid of pine pollen and purified by the combination of DEAE cellulose ion exchange chromatography and Sephadex G-100 gel filtration. The purification steps resulted in about 59 fold purification with 37 % recovery of the enzyme activity. The purified enzyme showed a single protein band on the acrylamide gel after electrophoresis and the molecular weight of the enzyme was estimated to be 47,000 dalton by gel filtration with a calibrated Sephadex G-100 column. Its enzyme activity was inhibited by a thiol-directed reagent, p-chloromercuribenzene sulfonic acid. The optimum pH of this enzyme was 8.0 and a typical Michelis-Menten type saturation pattern with increasing concentration of p-nitrophenylacetate was obtained. From the double reciprocal plot its Km value was calculated to be 166 μM. This enzyme also hydrolyzed p-nitrophenylpalmitate but not tripalmitin.

리튬이차전지용 천연흑연의 표면 개질에 대한 전기화학적 특성 분석

박진규 ( Jin-kyu Park ),서진성 ( Jin-seong Seo ),조병원 ( Byung-won Cho ),나병기 ( Byung-ki Na ) 충북대학교 산업과학기술연구소 2020 산업과학기술연구 논문집 Vol.34 No.2

The common anode material of commercial lithium secondary battery is natural graphite that has excellent cycle stability campared with other anode materials such as metal(Sn, Sb, Al, Si etc) and low production cost. But this material has the problems like irreversible capacity and poor high rate capability, which depends on surface characteristics of the material. In this study surface fluorination has been proposed to improve electrochemical performance of natural graphite. Surface fluorination was performed at different temperature and time using pure F2 and F2/He mixture gas. The electrochemical properties of fluorinated natural graphite were inverstigated with scanning electron microscopy(SEM), particle size distribution (PSD), BET surface area, cyclic voltammetry(CV) and electrochemical charge-discharge test. The fluorination of natural graphite enhanced the density and the stability of SEI film, and improved the cycle stability and the cycle life of lithium-ion secondary batteries.