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엄나무 (Kalopanax pictus Nakai) 줄기 추출물이 In vitro 반추위 발효와 메탄저감에 미치는 영향
김재성,황문석,김용채,윤영만,배귀석,김창현,Kim, Jae Seong,Hwang, Moon Seok,Kim, Yong Chae,Yoon, Young-Man,Bae, Gui Sek,Kim, Chang-Hyun 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.3
An experiment was conducted to examine the effects of Kalopanax pictus Nakai (Kalopanax) on in vitro rumen fermentation and methane (CH4) reduction. Kalopanax trunk was extracted with 70% ethanol and 70% methanol. Rumen fluid, alfalfa hay and buffer (control: C) supplemented with 0.3% Kalopanx juice (T1), 0.3% ethanol extract (T2) and 0.3% methanol extract (T3) in the total volume of culture medium were incubated at $38^{\circ}C$ for 24h and 48h. Rumen pH was lower in all Kalopanax treatments during all incubations than that in control (p<0.05). Total VFA and total gas production in T2 and T3 was significantly higher than that in C at 48h incubation (p<0.05). Ammonia-N was decreased in all treatments compared with C during the incubation periods (p<0.05). At 24h incubation, $CH_4$ contents were significantly reduced by both alcohol extracts. It is concluded that supplementing Kalopanax extracts can stimulate ruminal fermentation of rumen microorganisms and inhibit methanogenesis.
운동형태에 따른 최대 운동이 심근 산소소비량과 혈중 젖산 농도의 변화에 미치는 영향
김재성,김성수,장경모,Kim, Jae-Seong,Kim, Sung-Soo,Jang, Kyung-Mo 대한물리치료과학회 2003 대한물리치료과학회지 Vol.10 No.1
The purpose of this study was to compare the effects of different exercise types including isokinetic, isometric, and istonic exercise of same exercise intensity on cardiopulmonary function and blood lactate level. The subjects of this study included 17 males college students. Each subjects after pretraining measurement performed isokinetic, isometric and isotonic exercise of 1 week interval using Cybex 6000 System. KBI-C and YSI 1500 were used to measure changes in cardiopulmonary function and blood lactate level respectively. First, comparing changes in RPP relative to exercise type, isokinetic group showed significant difference between before exercise ($7.08{\pm}1.12mg/kg/min$) and post exercise ($18.98{\pm}1.75mg/kg/min$). Isometric group showed that significant difference between before exercise($7.89{\pm}0.98mg/kg/min$) and post exercise($20.22{\pm}2.41mg/kg/min$). Isotonic group showed significant difference between before exercise ($8.14{\pm}0.11mg/kg/min$) and post exercise ($19.84{\pm}2.30\;mg/kg/min$). Second, comparing changes in blood lactate level relative to exercise type, isokineic group showed significant difference between before exercise ($2.99{\pm}0.65mmol$) and post exercise ($6.55{\pm}6.55mmol$). Isometric group showed significant difference between before exercise($1.71{\pm}5.48mmol$) and post exercise ($5.48{\pm}1.97mmol$). Isotonic group showed significant difference between before exercise($1.16{\pm}0.48mmol$) and post exercise($5.21{\pm}1.28mmol$). The results of this study indicate significant differences RPP in isometric exercise and significant differences blood lactate in isotonic exercise.
2013년 하계 국내 연안에서 출현하는 Alexandrium 속 와편모류의 종 다양성
김재성,박경우,윤석현,임월애,유영두,성경아,이원호,KIM, JAE SEONG,PARK, KYUNG WOO,YOUN, SEOK HYUN,LIM, WEOL AE,YOO, YEONG DU,SEONG, KEYONG AH,YIH, WON HO 한국해양학회 2016 바다 Vol.21 No.4
We investigated the occurrence of the dinoflagellate genus Alexandrium in the nineteen Korean coastal sites from July to October 2013. Alexandrium-like planktonic cells were microscopically observed only in four out of the 19 sampling sites. From the samples containing Alexandrium-like cells 22 clonal cultures of Alexandrium species were established by single cell or single chain isolation method. Taxonomic identity of the 4 different strains ascertained by the robust analyses of morphological and molecular genetic characteristics were confirmed to be A. catenella, A. affine, A. fraterculus and an unidentified Alexandrium sp. for which strain WEB-Alex-01 was assigned. It was ascertained that in spite of hot summer diverse Alexandrium species attaining up to four were distributed in the study area, in contrast with the long empirical recognition that the emergence of Alexandrium species is restricted to cooler seasons like spring or autumn in Korean coastal waters. Morphology and genetic characteristics of Alexandrium sp. strain WEB-Alex-01 are different from any other previously reported Alexandrium species from Korean seas, which implies that further studies on taxonomic, physiological, ecological and toxicological properties of the newly recorded Alexandrium species are needed. 국내 연안에서 하계에 출현하는 Alexandrium 속 와편모류의 종 다양성 연구를 위해, 2013년 7월부터 10월까지 19개 정점에서 해수 시료를 채취하였고, 이 가운데 4개 지역에서 Alexandrium 속의 와편모류가 확인되었다. 해당 4개 해수 시료에 단세포/단세포사 분리법을 적용하여, 총 22 종류의 Alexandrium 단종배양체를 확립하였다. 이 가운데 4종류의 Alexandrium 단종배양체에 대한 형태학 및 유전학적 특성을 분석한 결과, 각각 A. catenella, A. affine, A. fraterculus 및 Alexandrium sp.(strain WEB-Alex-01)인 것으로 확인되었다. 우리나라 연안에서 일반적으로 Alexandrium이 춘계 또는 추계의 중수온기에 주로 출현하는 것으로 알려진 바와는 대조적으로, 다양한 Alexandrium 종들이 하계에도 출현함을 확인하였다. 미동정 Alexandrium sp.(strain WEB-Alex-01) 종주는 국내 최초 보고 종으로서, 이 단종배양체에 대한 분류, 생리, 생태, 독성 등에 관한 추가적인 연구가 필요하다.
피스테리아와 피스테리아 유사종의 전복 유생 섭식 특성과 생존을 연구
김재성,이창원,이희만,정해진,Kim, Jae-Seong,Lee, Chang-Won,Lee, Hee-Mahn,Jeong, Hae-Jin 한국해양학회 2009 바다 Vol.14 No.3
종속영양 와편의류인 Pfiesteria 종과 이와 유사한 형태의 Pfiesteria-like 종간의 생태적 지위차를 확인하기 위해, Pfiesteria piscicida, Cryptoperidininopsis brodyi, Stoeckeria algicida 3종에 전복유생(abalone larvae)을 투입하여 그들의 섭식 여부를 확인하고, 이들의 농도변화에 따른 전복 유생의 생존율 변화를 확인하였다. P. piscicida와 C. brodyi는 부화 1일차의 전복 유생을 직접 공격하여 치사 시킨 후 peduncle이라는 섭식기관을 이용하여 개체 전체를 섭식하는 것으로 밝혀졌다. 하지만 형태적으로 이들과 매우 유사하고 peduncle도 가진 S. algicida는 전복 유생을 직접 공격하거나 섭식하지 않았다. 전복 유생의 생존율은 P. piscicida와 C. brodyi의 초기 투입농도가 증가함에 따라 상대적으로 낮아졌다. 이러한 결과는 형태적으로 매우 유사한 종속영양성 와편모류일지라도 그들이 가지는 생태적지위는 매우 다르며, 보다 명확한 해양 생태계 먹이사슬을 이해하기 위해서는 종(species) 수준의 명확한 종 동정연구가 필요함을 제시해 준다. To investigate the difference in the ecological niches between Pfiesteria piscicida and Pfiesteria-like species (Cryptoperidininopsis brodyi and Stoeckeria algicida), we have observed the feeding behavior of three potential predators on abalone larvae and measured the survival rates of abalone larvae as a function of initial predator concentration. When the predators were mixed with abalone larvae, P. piscicida and C. brodyi became active and exhibited attacking behavior on abalone larvae within a few seconds. They could ingest whole soft body of abalone larvae using a peduncle. In contrast, feeding and attacking behavior were not observed from S. algicida. Survival rates in abalone larvae decreased with an increase of the initial concentration of P. piscicida and C. brodyi. These results indicate that Pfiesteria and Pfiesteria-like species displaying the very same shape and size have different ecological niche in the marine food webs, which implies that identification to species level is definitely important to understand and discriminate the ecological roles of them.
소프트웨어 거래의 UCC 제2편의 적용에 관한 연구 - 미국의 판례를 중심으로 -
김재성,Kim, Jae-Seong 한국무역상무학회 2002 貿易商務硏究 Vol.18 No.-
Owing to a tremendous progress of information technology we have faced a great change of a society. This change has happened to almost all of a society. As a result intangible goods are also included as a major concern in international trade. We may say U.S. is one of the countries transaction of software is the most flourishing. I have tried to classified the cases of software transaction in U.S. into five groups. As a result I have known the UCC had been applied to the almost transaction of standard software. However UCC had not been applied to custom software and data processing. In these days UCITA which is very similar to a previous proposal UCC 2B has been presented. This is a contract law that would apply to computer software, multimedia products, and databases. It has been designed to create a uniform commercial contract law for those products. Now therefore we should make observation of a tendency about transaction of software in U.S. The reason is that we pay attention to the symbol and dormant power of U.S. in international trade.