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신재희,박진우,여신일,노우창,김문규,김정철,서조영,Shin, Jae-Hee,Park, Jin-Woo,Yeo, Shin-Il,Noh, Woo-Chang,Kim, Moon-Kyu,Kim, Jung-Chul,Suh, Jo-Young The Korean Academy of Periodontoloy 2007 Journal of Periodontal & Implant Science Vol.37 No.2
치주인대세포는 시험관적 실험에서 광물화 결절형성을 유도할 수 있으므로 광물화 결절형성에 관여하는 유전자들을 특이하게 발현할 것으로 여겨진다. 이에 본 실험은 cDNA microarray를 이용한 동시 유전자분석을 시행하여 치주인대세포의 분화에 의한 광물화 결절형성시 나타나는 유전자의 특징적 발현 양상을 알아보고자 하였다. 교정치료를 목적으로 경북대학교병원에 내원한 환자의 제일소구치를 발치하여 통상적 방법으로 치주인대세포를 분리, 배양하였고, 3세대의 치주인대세포를 사용하여 실험을 시행하였다. 치주인대세포를 100mm 배양접시에 넣고 배양하여 매 2일 마다 배지를 교환해 주고, 10% FBS만을 투여한 대조군으로, ascorbic acid $(50\;{\mu}g/ml)$, ${\beta}-glycerophosphate$ (10 mM) 및 100 nM dexamethasone을 투여한 군을 실험군으로 하였다. 배양된 치주인대세포에 ascorbic acid, ${\beta}-glycerophosphate$, 그리고 dexamethasone을 투여한 실험군에서 21일째 광물화된 결정을 관찰할 수 있었으나 대조군에서는 관찰할 수 없었다. 3063개의 유전자를 분석한 결과 35개 유전자가 대조군에 비해 2배이상 발현이 증가하였고, 38개 유전자는 2배이상 발현이 감소하였다. 형태학적 검사에서 보여준 바와 같이 광물화 형성과정시 관여하는 JGF-2과 IGFBP2와 같은 유전자가 실험군에서 증가하였으며, 세포골격과 세포외기질 형성에 관여하는 proteogycan 1, fibulin-5, keratin 5, ${\beta}-actin$, ${\alpha}-smooth$ muscle actin, capping protein 등도 발현이 실험군에서 증가하였다. 한편 periostin and S100 calcium-binding protein A4는 대조군에서 오히려 높게 나타나므로 이는 배양된 치주인대세포가 그 자체의 표현형을 유지하고 있음을 보여 주고 있다. 그 외 apoptosis를 유발시키는데 관여하는 Dkk-1와 Nip3는 실험군에서 높게 발현되었고, apoptosis를 억제시키는데 관여하는 Btf와 TAX1BP1는 오히려 낮게 발현됨을 알 수 있으므로 이는 실험군에서 치주인대세포가 골아세포로의 분화되었음을 나타낸다. Periodontal ligament (PDL) cells have been known as multipotential cells, and as playing an important rolesin periodontal regeneration. The PDL cells are composed of heterogeneous cell populations which have the capacity to differentiate into either cementoblasts or osteoblasts, depending on needs and conditions. Therefore, PDL cells have the capacity to produce mineralized nodules in vitro in mineralization medium which include ascorbic acid, ${\beta}$-glycerophosphate and dexamethasone. In spite of these well-known osteoblast like properties of PDL cells, very little is known about the molecules involved in the formation of the mineralized nodules in the PDL cells. In the present study, we analysed gene-expression profiles during the mineralization process of cultured PDL cells by means of a cDNA microarray consisting of 3063 genes. Nodules of mineralized matrix were strongly stained with alizarin red S on the PDL cells cultured in the media with mineralization supplements. Among 3,063 genes analyzed, 35 were up-regulated more than two-fold at one or more time points in cells that developed matrix mineralization nodules, and 38 were down-regulated to less than half their normal level of expression. In accord with the morphological change we observed, several genes related to calcium-related or mineral metabolism were induced in PDL cells during osteogenesis, such as IGF-II and IGFBP-2. Proteogycan 1, fibulin-5, keratin 5, ,${\beta}$-actin, ${\alpha}$-smooth muscle actin and capping protein, and cytoskeleton and extracellular matrix proteins were up-regulated during mineralization. Several genes encoding proteins related to apoptosis weredifferentially expressed in PDL cells cultured in the medium containing mineralization supplements. Dkk-I and Nip3, which are apoptosis-inducing agents, were up-regulated, and Btf and TAXlBP1, which have an anti-apoptosis activity, were down-regulated during mineralization. Also periostin and S100 calciumbinding protein A4 were down-regulated during mineralization.
The micro-current stimulation system for cellular inflammatory modulation study
Jae-Hee Shin(신재희),Min-Ho Hwang(황민호),Hyo-Geun Nam(남효근),Hyeong-Guk Son(손형국),Seung-Min Baek(백승민),Chang-Min Yoo(유창민),Jeong-Hun Park(박정훈),Jin-Su Jeong(정진수),Jae-Won Lee(이재원),Hyuk Choi(최혁) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6
Electric stimulation (ES) is known to affect cell migration, proliferation, inflammation regulation, and physiological activities. However, there exist strong attenuation of electric field (EF) when conventional ES pass through tissue, and need of direct contact of target tissue and electrode has been magnified. In order to overcome these limitations, we have designed and fabricated precise ES device (MCS system), which can support with precise current density and adjustable frequency. Then we applied to degenerative disc disease (DDD) model in vitro. We showed that inflamed annulus fibrosus (AF) cells, which mimicked DDD model, express inflamatory cytokines such as interleukin (IL)-6, and –8 as contrasted with normal AF cells. But inflamed AF cells stimulated with ES and showed decrease in inflammatory cytokines. These results suggests that ES can be a therapeutic tool for inflammatory modulation and MCS is expected to be applied in many cell types and foster diverse applications of ES study fields.
〈학술논문〉 삼각형 형상의 단일수평채널 내에서 응축되는 증기의 열 특성
신재희(Jae Hee Shin),황교식(Kyo Sik Hwang),하효준(Hyo Jun Ha),장석필(Seok Pil Jang),이기우(Ki Woo Lee) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11
In the papermaking processes it is well known that the dryer uses the largest amount of energy and has costly components compared with other devices. Many researchers thus conducted to reduce energy and cost of the dryer. Recently Argonne national laboratory has developed the concept of a multiport cylinder dryer that the steam flows along longitudinally oriented mini-channels which are close to inner surface of cylinder dryer. They reported that the innovative design minimizes the condensate layer thickness. However, systematically experimental data are not available. So in this paper we will conduct experiments to study thermal characteristics of condensing steam in a single horizontal mini-channel with triangular shape, representative of a multiport cylinder dryer. Based on the results, we confirm previous results and observe the condensing heat transfer coefficient in the single horizontal mini-channel with triangular shape.
Min Ho Hwang(황민호),Jae Hee Shin(신재희),Hyo Geun Nam(남효근),Hyeong Guk Son(손형국),Seung Min Baek(백승민),Chang Min Yoo(유창민),Jeong Hun Park(박정훈),Jin su Jeong(정진수),Jae won Lee(이재원),Hyuk Choi( 최혁) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6
Intervertebral disc degeneration (IVD) is one of the important causes of low back pain and is associated with inflammation induced by interaction between macrophages and the human annulus fibrosus (AF) cells. Low level light therapy (LLLT) has been widely known to regulate inflammatory reaction. However, the effect of LLLT on macrophage-mediated inflammation in the AF cells has not been studied till date. The aim of this study is to mimic the inflammatory microenvironment and to investigate the anti-inflammatory effect of LLLT at a range of wavelengths (405, 532 and 650 nm) on the AF treated with macrophage-like THP-1 cells conditioned medium (MCM) containing proinflammatory cytokines and chemokines (interleukin-1beta, tumor necrosis factor-alpha, interleukin-6 and 8). We observed that AF cells exposed to MCM secrete significantly higher concentrations of IL-6, IL-8, IL-1b and TNF-a. LLLT markedly inhibited secretion of IL-6 at 405 nm in a time-dependent manner. Level of IL-8 was significantly decreased at all wavelengths in a time-dependent manner. We showed that MCM can induce the inflammatory microenvironment in AF cells and LLLT selectively suppressed IL-6 and 8 levels. The results indicate that LLLT is a potential method of IVD treatment and provide insights into further investigation of its antiinflammation effect on IVD.
강승호(Seung ho Kang),천신(Shin Chun),김국한(Guk Han Kim),김경수(Kyoung soo Kim),백승민(Seung min Back),신재희(Jae hee Shin),조가은(Ga eun Jo),황민호(Min ho Hwang),최혁(Hyuk Choi) 대한전기학회 2014 대한전기학회 학술대회 논문집 Vol.2014 No.2
The pneumatic CPM (continuous passive motion) device was developed in our group. We mainly focused on fingers and wrists of patients. Rehabilitation devices require the highest standards due to the device directly interacts with patients. Using air pressure system, lightweight, compact, and cost effective CPM was designed and manufactured. We also human pilot clinical study was performed. The CPM would be utilized in hospital and at home as well.
Computational fluid dynamics of NiTi stents for Cerebral Aneurysm prevention
Hyo-Geun Nam(남효근),Chang-Min.Yoo(유창민),Jae-Hee Shin(신재희),Min-Ho Hwang(황민호),Seung-Min Baek(백승민),Jung-Hoon Park(박정훈),Jin-Su Jeong(정진수),Jae-Won Lee(이재원),Hyuk Choi(최혁) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6
Stents are significant medical devices broadly used in the prevention of cerebral aneurysm rupture. The performance of stents depends on their mechanical properties and cell configuration, thus, the aim of this study is the optimization of stent design and test the hemodynamic properties by using computational solid mechanics and computational fluid dynamics. In performance tests, computer-based cerebral aneurysm models of human brain were tested. closed-cell type was selected as the strut configuration, and nitinol was chosen as the material due to its flexibility. Three types of ideal samples with different cell configurations were manufactured. There were over 30% difference in results of flexibility in computational solid mechanics analysis. Moreover, in cerebral aneurysm model simulation, we investigated that stent insertion eased the hemodynamic factors of the cerebral aneurysm and reduced the flow velocity influx into the sac. A reduction in flow velocity led to a 50–60% reduction in wall shear stress, which is expected to aid aneurysm rupture prevention under clinical conditions.
Stabilization of the surface of nitinol stent for cerebral aneurysm prevention
Chang-Min.Yoo(유창민),Hyo-Geun Nam(남효근),Je-Hee Shin(신재희),Min-Ho Hwang(황민호),Seung-Min Baek(백승민),Hyeong-Guk Son(손형국),Jeong-Hun Park(박정훈),Jin-Su Jeong(정진수),Jae-Won Lee(이재원),Hyuk Choi(최혁) 대한전자공학회 2016 대한전자공학회 학술대회 Vol.2016 No.6
Nikel titanium shape memory alloys (NiTi) are promising materials due to their shape memory effect. The disadvantage of the nickel is the toxicity, there are too many restrictions on the use of the human body. Hence, if the proper surface modification is chosen, the surface of NiTi can be tranquilized and smooth. Also, toxic chemicals can be protected by oxide film. The polishing method we have used is electro-polishing which is simple enough, economical and can handle wide range at once. In this study, we are surface modification NiTi by surface electro-polishing. The polishing method we have used is electro-polishing which is simple enough, economical and can handle wide range at once. We have used AFM(atomic force microscopy), SEM(scanning electron microscopy), OM(optical microscope) to analyze the roughness, and formation of surface of NiTi stent. To measure corrosion resistance, steady potentiostat was used to corrosion test. Our study group have concluded that condition of 40V 10seconds of electro-polishing showed the optimal stabilization of the surface.