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Effect of Methylotrophic Bacteria in Seedling Development of Some Crops under Gnotobiotic Condition
In-Soo Hong(홍인수),Jun-Seok Kim(김준석),Min-Kyong Lee(이민경),Woo-Jong Yim(임우종),Md. Rashedul Islam,Hari P. Deka Boruah,Puneet Singh Chauhan,Guang-Hyun Han(한광현),Tong-Min Sa(사동민) 한국토양비료학회 2009 한국토양비료학회지 Vol.42 No.4
농업유용미생물을 이용한 원예 및 채소작물의 건전 유묘 생산을 위하여 본 연구에서는 growth pouch 실험을 통하여 6가지 식물생육촉진 미생물을 접종하여 작물의 초기 뿌리 생장에 미치는 영향을 살펴 보았다. 본 실험에 사용한 6가지 균주는 Methylobacterium oryzae CBMB20, Methylobacterium phyllosphaerae CBMB27, Methylobacterium suomiense CBMB120, Methylobacterium strains CBMB12, CBMB15와 CBMB17이었다. 대조구와 비교했을 CBMB12, CBMB17, 및 CBMB20접종은 상추 초기 뿌리 생육에 유의성있는 효과를 보였고, CBMB102접종은 배추 초기 뿌리 생육에 유의성있는 효과를, CBMB27접종은 토마토의 초기 뿌리 생육에 유의성있는 효과를 보였다. 또한 Methylobacterium suomiense CBMB120접종은 오이, 토마토, 배추, 그리고 Methylobacterium strain CBMB12접종은 고추의 뿌리 초기 생장을 크게 촉진시켰음을 알 수 있었다. 위의 결과를 통하여 실험한 균주들을 각각의 작물 육묘 포트에 처리할 때에도 유묘의 생장속도를 촉진 시킷수 있음을 예상할 수 있다. Healthy seedling generation is the major concern in overcoming adverse effects of biotic and abiotic stresses during tender stage of development in vegetables and horticultural crops. Because of this, priority is given to research leading to the generation of healthy seedlings in crops subjected to transplanting and bedding. In this study, growth pouch experiments were conducted to determine the effect of inoculation of six different strains of Methylobacterium sp. namely, M. oryzae CBMB20, M. phyllosphaerae CBMB27, M. suomiense CBMB120, and Methylobacterium strains CBMB12, CBMB15 and CBMB17 on the seedling development of the vegetable crops cabbage, Chinese cabbage and cucumber; and horticultural crops tomato and red pepper. Crops treated with the test strains generally showed higher seedling dry matte accumulation compared to the control. Significantly higher accumulation was exhibited by CBMB12, CBMB17, and CBMB20 in cabbage, as well as for CBMB27 and CBMB120 on tomato and Chinese cabbage, respectively. Furthermore, all the strains promoted root elongation in cucumber and tomato seedlings while in Chinese cabbage and red pepper, root elongation was observed with CBMB120 and CBMB12 inoculation, respectively. Large scale nursery study is needed to develop a thorough protocol for healthy seedling development with the use of these strains.
갑상선 악성종양에서 갑상선 전절제술 후 방사성 요오드 치료의 효과
홍인수(In Soo Hong),강성준(Seong Joon Kang),안희철(Hee Cheol Ahn) 대한두경부종양학회 1998 대한두경부 종양학회지 Vol.14 No.1
Objectives: The response rate of the radioactive iodine(RI) therapy with low dose was variable. Only a few studies reported the response and complication rate with high dose. The goal of this study was to access the ablation and complication rate after high dose of RI therapy (more than 100mCi) and to evaluate the factors affect the results. Material and Methods: During a period of 12 years, 225 patients received high dose of RI from 100 to 200mCi depending on the RI uptake in the whole body after total thyroidectomy. 100mCi of RI was given to 123 patients for ablation who showed the uptake only in the thyroid bed. 150mCi was given to another 84 patients for ablation who had uptake confined to the neck. The other 21 patients took more than 200mCi of RI because the whole body scan showed distant metastasis. Among these patients, the ablation and the complication rate was investigated. Results: Elevated level of the serum thyroglobulin(Tg) decreased less than 5ng/ml after RI therapy in all patients except two in the first group. The second group showed reduction of the serum Tg in 93%. Eighteen of the 21 patients in the third group are still alive after RI therapy. There were no fatal complications after high dose RI therapy and most of the complications were minimal and transient. The complication rate was not related with the dose of RI, age, sex, DNA flowcytometry, serum thyroglobulin level and the extent of node dissection. Conclusion: We concluded that RI therapy with high dose was very effective for thyroid ablation after operation and it also showed excellent results with minimal complications for treatment of metastatic lesions.
간세포암환자에서 C-DDP 단독 혹은 C-DDP와 Doxorubicin의 병합 간동맥화학요법의 비교
이종인(Chong In Lee),장우익(Woo Ick jang),김현수(Hyun Soo Kim),신승준(Seung Joon Shin),이성우(Seong Woo Lee),이동기(Dong Ki Lee),권상옥(Sang Ok Kwon),심영학(Young Hak Sim),김영주(Young Ju Kim),조휘율(Whee Yul Cho),홍인수(In Soo Hong) 대한소화기학회 1994 대한소화기학회지 Vol.26 No.2
N/A Most of hepatocellular carcinomas, especially in Korea, are considered as inoperable due to extrerne tumor extension at the tirne of diagnosis and underlying advanced liver cirrhosis. The normal liver receives a dual blood supply from the hepatic artery and the portal vein whereas hepatoma is fed preferentially by the hepatic artery. With the background above mentioned, hepatic arterial chemotherapy or chemoembolization in hepatocellular carcinoma has been regarded as an important treatment modality for unresectable hepatocelluar carcino- ma. It has been found that the lipid lymphographic agent such as Lipiodol(ethiodized oil), which can be mixed with chemotherapeutic agents, is selectively retained within hepatocelluar carcinoma when it is injected into the hepatic artery. Based on recent promising therapeutic results in hepatocelluar carcinoma treated with hepatic arterial C DDP infusion combined with Lipiodol, we studied to elucidate the therpeutic efficacy of the hepatic arterial chemotherapy with C DDP combined with Lipiodol and to compare the differences in thera- peutic efficacy between C-DDP rnonotherapy group (Group 1) and C-DDP/doxorubicin combi- nation therapy group(Group 2). In all 42 cases there were 1 complete response(2%), 4 partial responses(10%), 7 rninimal responses(17%), 15 stab1e diseases(36%) and 15 progressive diseases (36%). There were no differences of response rate and survival duration between Group 1 and Group 2, and there was a significant difference of survival duration between responder group (CR+PR+MR) and nonresponder group(SD+PD) (P<0.01). In conclusion hepatic arterial chemotherapy with C DDP and/or doxorubicin and Lipiodol appears to have relatively weak activity in patients with hepatocellular carcinoma. (Korean J Gastroenterol 1994; 26: 298 304)
방사선 치료에 따른 99mTc - pertechnetate를 이용한 이하선과 악하선 기능 검사의 유용성
홍인수(In Soo Hong),성기준(Ki Joon Sung),김명순(Myung Soon Kim),김종진(Jong Jin Kim),이종영(Jong Young Lee),박진숙(Jin Sook Park) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.3
N/A Purpose: To assess the functional change of salivary gland, recovery of salivary destruction, difference of susceptibility to irradiation between the parotid and submandibular gland, correlation between the xerostomia and excretory rate by irradiation in salivary Tc-99m-pertechnetate in patients with head and neck cancer. Materials and Methods: We evaluated 19 patients with head and neck cancer who Tc-99m-pertechnetate salivary gland scan before, during and after radiotherapy. We measured the uptake of radioactivity every 5 minutes for 60 minutes after injection of Tc-99m-pertechnetate(370MBq). We also evaluated the first excretory function stimulated by 5% citric acid at 30 minutes after injection of Tc-99m-pertechnetate and then the second excretory function stimulated by 10% citric acid at 45 minutes. Results: The mean uptake curves showed a sharp increase at the 1st week of radiotherapy and then showed a gradual decrease afterwards during radiotherapy. And the mean uptake curves after radiotherapy persistently decreased on follow up scans without being recovered. The excretory rates decreased gradually as radiation dose increased and were not recovered after radiotherapy. Our study was not satisfactory enough to show the difference of susceptibility to irradiation between the parotid and salivary gland and the correlation between the symptom of xerostomia and the change of the excretory rates. Conclusion: The parotid and submandibular gland scans using Tc-99m- perechnetate are useful in the assessment of the functional change of the salivary gland by radiotherapy and predicted the functional change of the salivary gland until 1 year-follow up scan. KW: Salivary gland abnormality, Salivary gland, radionuclide studies, Salivary gland, therapeutic radiology, Tc-99m-pertechnetate
이명섭(Myeong Seob Lee),김응조(Eung Jo Kim),홍인수(In Soo Hong),성기준(Ki Jun Sung),박현주(Hyun Ju Park) 대한핵의학회 1992 핵의학 분자영상 Vol.26 No.1
N/A Miliary Tuberculosis is an illness produced by acute dissemination of tubercle bacilli via the blood stream. In chest roentgenogram, a diffuse miliary infiltrates are usually seen, but normal or suspicious ground glass pattern also can be seen in early manifestation. Ten patients of miliary tuberculosis who underwent whole-body 67Ga-citrate scintigraphy were evaluated retrospectively to study usefulness of Ga-scan for early diagnosis of miliary Tbc and evaluation of disease activity. All of ten patients demonstrated significantly diffuse bilateral pulmonary uptakes on 48 hours image. All of three patients of ground-glass pattern in chest roentgemogram also demonstrated increased uptakes. In the statistical analysis, the severity of chest roentgenographic findings showed positive correlation with the activity on Ga-scan. These results suggest that Gallium scan is useful for diagnosis of early miliary tuberculosis and for evaluation of disease activity on follow-up examination of miliary tuberculosis of lung.