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해양배양기 내 중탄산염 공급에 따른 Tetraselmis sp. KCTC12432BP 증식에 관한 연구
조용희,신동우,이상민,전효남,류영진,이종찬,임상민,이철균,Cho, Yonghee,Shin, Dong-Woo,Lee, Sangmin,Jeon, Hyonam,Ryu, Young-Jin,Lee, Jong-Chan,Lim, Sang-Min,Lee, Choul-Gyun 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2
The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.
영흥도에서 분리된 Phaeodactylum tricornutum의 증식 및 Monounsaturated fatty acid 관련 지방산 조성 분석
이상민,조용희,신동우,전효남,류영진,임상민,이철균,Lee, SangMin,Cho, Yonghee,Shin, ong-Woo,Jeon, Hyonam,Ryu, YoungJin,Lim, Sang-Min,Lee, Choul-Gyun 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2
Oxidation stability and cold fuid property are considered as the most important factors for determining biodiesel quality. Among the fatty acids, monounsaturated fatty acid satisfy both oxidation stability and cold flow property of biodiesel quality standards. Microalgae with high monounsaturated fatty acid contents is have some benefit for producing to produce biodiesels with satisfying quality standards. In this study, monounsaturated fatty acid contents of a isolated microalga from Youngheung island was analyzed. Phaeodactylum tricornutum was isolated by streaking, and growth rate and fatty acid composition of the algae were investigated. Total FAME contents were consisted of 26% of saturated fatty acids, 43% of monounsaturated fatty acids, and 18% of polyunsaturated fatty acids. The contents of monounsaturated fatty acid were especially high in the Phaeodactylum species. This result implies that the FAMEs from P. tricornutum may contribute to improve the oxidation stability and cold flow property of biodiesel.