가시오가피의 삽목번식방법 (揷木繁殖方法)

박호기(Ho Ki Park),박문수(Moon Soo Park),김태수(Tai Soo Kim),최인녹(In Leok Choi),장영선(Yeong Sung Jang),김규성(Gue Seong Kim) 한국약용작물학회 1994 한국약용작물학회지 Vol.2 No.2

Eleutherococcus senticocus MAXIM containing compounds for a cordial and lumbago is a native plant in this country. However it is generally difficult to propagate them by seed. This study was carried out to improve propagation efficacy by cutting method using Eleutherococcus senticocus MAXIM collected Mt. Odae for three years(`90∼`92). Rooting was delayed by 3 to 12 days but callus formation and rooting percentage was high by 75%, 30% respectively in the treatment of Rooton-F powder, a chemical for rooting promotion, compared with non-treatment. Rooting percentage and rooting characters were not differenced by treatment for remove rooting inhibition compounds and soil-media mixured by 1:1 of vermiculite and perlite was suitable for elevating rooting ratio. A. chiisanensis and A. sieboldianum were rooted well in any tune to be cut, while rooting activity of E. senticosus was different by time to be cut. Rooting ratio of E. senticosus was 20%, 36% and 60% when cutter March 20, July 20 and September 20, respectively.

기내 대량 생산 반하 (半夏) 종구의 (種球) 포장 배양기술 연구

박호기(Ho Ki Park),김태수(Tai Soo Kim),박문수(Moon Soo Park),최인녹(In Leok Choi),장영선(Yeong Sun Jang),박근용(Keun Yong Park) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

This study was carried out to determine the optimum planting time for in vitro multiplied tuber of Pinellia ternatat (Thunb.) Breit. The tubers were planted on April 20, May 20, June 20, July 20, August 20 and September 20 in 1990, Emergence ratios were 68 to 87% in any planting time except planting on July 20. The number of tubers per ㎡ at harvest in plantings on May 20 and June 20 were significantly higher with 1,110 and 1,021, respectively, while in plantings after July 20, those were drastically decreased. As compared with fresh yield of planting on April 20(352㎏/l0a), that of May 20 was 109% and June 20 was 103.%, while those of after July 20 were from 41% to 19%. There was a highly positive correlation between dry tuber yield and the number of tubers per ㎡(r==0_991**). Tuber yields for co㎜ercial use(diameter over 7.1㎜) were high in planting on May 20(322㎏/10a) and on June 20(299㎏/10a). It was suggested that optimum field planting time for in vitro multiplied tuber of Pinellia ternata (Thunb.) Breit was from May 20 to June May 20.

기내(器內) 대량(大量) 생산(生産) 반하(半夏) 종구(種球)의 포장(圃場) 재배기술(裁培技術) 연구(硏究) I. 파종시기(播種詩期)가 생육(生育)과 괴경형성(塊莖形成) 및 수량(收量)에 미치는 영향(影響)

박호기,김태수,박문수,최인록,장영선,박근용,Park, Ho-Ki,Kim, Tai-Soo,Park, Moon-Soo,Choi, In-Leok,Jang, Yeong-Sun,Park, Keun-Yong 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

조직(組織) 배양(培養)을 통(通)하여 대량(大量) 생산(生産)된 반하(半夏) 종구(種球)의 포장재배(圃場裁培) 파종(播種) 적기(適期)를 구명(究明)하기 위하여 '90년(年) 4월(月) 20일(日), 5월(月) 20일(日), 6월(月) 20일(日), 7월(月) 20일(日), 8월(月) 20일(日) 및 9월(月) 20일(日)에 파종(播種)하여 출아특성(出芽特性), 생육특성(生育特性), 괴경형성(傀莖形成) 및 수량(收量)에 관련된 몇 가지 형질(形質)에 대하여 2개년간(個年間)$('90{\sim}'91)$ 검토(檢討)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 파종기별(播種期別) 출아소요기간(出芽所要期間)(파종(播種)${\sim}$출아(出芽))은 $9{\sim}26$일(日) 이었으며 파종기(播種期)가 빠른 4월(月) 20일(日)이 26일(日), 온도(溫度)가 높은 시기(時期)인 8월(月) 20일(日) 파종(播種)이 9일(日)로 가장 짧았으며 이 기간(期間)의 적산온도(積算溫度)는 $256{\sim}334^{\circ}C$이었다. 2. 파종기별(播種期別) 출아율(出芽率)은 $68{\sim}87%$로 대체로 좋았으나 7월(月) 20일(日) 파종(播種)은 고온(高溫) 및 한발(旱魃)의 영향(影響)으로 저조(低調) (55%)하였다. 3. 파종기별(播種期別) 재배기간중(栽培期間中)(2년(年)) 생육과정(生育過程)은 4월(月) 20일(日), 5월(月) 20일(日), 6월(月) 20일(日) 및 7월(月) 20일(日) 파종(播種)은 4회(回) 생육(生育)을, 8월(月) 20일(日)과 9월(月) 20일(日) 파종(播種)은 3회(回) 생육(生育)하였고, 총(總) 생육기간(生育期間)은 125일(日)${\sim}$239일(日)로 파종기(潘種期)가 빠를 수록 길었으며 9월(月) 20일(日) 파종(播種)은 125일(日)에 불과(不過)하였다. 4. 파종기별(潘種期別) 수확(收穫) 당시(當時)의 초장(草長), 괴경장(塊莖長) 및 괴경폭(塊莖幅)은 4월(月) 20일(日)${\sim}$6월(月) 20일(日) 파종(播種)은 큰 반면(反面) 7월(月) 20일(日) 이후(以後) 파종(播種)은 극히 적었으며 초장(草長)과 괴경장(塊莖長), 괴경폭(塊莖幅) 및 괴경수량(塊莖收量)과는 유의(有意) 상관(相關)이 인정(認定)되었다. 5. 파종기별(播種期別) 수확당시(收穫當時)의 $m^2$당(當) 주수(株數)는 5월(月) 20일(日)과 6월(月) 20일(日) 파종(播種)은 다른 파종기(播種期)에 비해 유의적(有意的)으로 많았으며(P<0.05) 7월(月) 20일(日) 이후(以後) 파종(播種)은 급멸(急滅)하였다. 6. 파종기별(播種期別) 생체수량(生體收量)은 4월(月) 20일(日) 파종(播種)(352kg/10a)에 비해 5월(月) 20일(日)이 9%(P<0.05), 6월(月) 20일(日)이 3% 증수(增收)하였고, 7월(月) 20일(日) 이하(以後) 파종(播種)은 $59{\sim}81%$까지 크게 감수(減收)하였으며 건물수량(乾物收量)도 같은 경향(傾向)이었다. 7. 파종기별(播種期訓) 상품가치(商品價値)가 있는 7.1mm이상(以上)의 괴경(塊莖)크기 분포(分布)는 5월(月) 20일(日) 파종(播種)이 322kg/10a, 6월(月) 20일(日) 파종(播種)이 299kg/10a으로 훨씬 많은 반면 7월(月) 20일(日)${\sim}$9월(月) 20일(日) 파종(播種)은 $55{\sim}117kg/10a$으로 극히 적었다. 8. 수량(收量)과 $m^2$당(當) 주수(株數)와는 고도(高度)의 상관(相關)$(r=0.992^{**})$이 인정(認定)되었으며 다수확(多收獲)을 위해서는 기내(器內) 생육(生産) 반하(半夏) 종구(種球)를 5월(月) 20일(日)${\sim}$6월(月) 20일(日)에 파종(播種)하는것이 가장 바람직하였다. This study was carried out to determine the optimum planting time for in vitromultiplied tuber of Pinellia ternata(Thunb.) Breit. The tubers were planted on April 20, May 20, June 20, July 20, August 20 and September 20 in 1990. Emergence ratios were 68 to 87% in any planting time except planting on July 20. The number of tubers per $m^2$ at harvest in plantings on May 20 and June 20 were significantly higher with 1,110 and 1,021, respectively, while in plantings after July 20, those were drastically decreased. As compared with fresh yield of planting on April 20(352kg /10a), that of May 20 was 109% and June 20 was 103%, while those of after July 20 were from 41% to 19%. There was a highly positive correlation between dry tuber yield and the number of tubers per $m^2(r=0.991^{**})$. Tuber yields for commercial use(diameter over 7.1mm) were high in planting on May 20(322kg /10a) and on June 20(299kg /10a). It was suggested that optimum field planting time for in vitro multiplied tuber of Pinellia ternata(Thunb.) Breit was from May 20 to June May 20.

기내(器內) 대량(大量) 생산(生産) 반하(半夏) 종구(種球)의 포장(圃場) 재배기술(栽培技術) 연구(硏究) II. 수확시기별(收穫時期別) 생육특성(生育特性), 괴경(塊莖)크기 및 수량(收量)

박문수,박호기,김태수,장영선,박근용,Park, Moon-Soo,Park, Ho-Ki,Kim, Tai-Soo,Jang, Yeong-Seon,Park, Keun-Yong 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

조직배양기법(組織培量技法)을 이용(利用)하여 대량증식(大量增殖)된 반하(半夏) 종구(種球)를 포장재배(圃場栽培)할 때 알맞은 수확시기(收穫時期)를 구명(究明)하기 위하여 파종후(播種後) 6개월(個月), 12개월(個月), 18개월(個月) 및 24개월(個月) 수확시(收穫時) 생육특성(生育特性), 괴경(塊莖)크기 분포(分布) 및 수량(收量)등에 관련(關聯)된 몇가지 형질(形質)에 대(對)하여 3년간(年間)('90${\sim}$'92년(年)) 조사(調査)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수확시기별(收穫時期別) 초장(草長) 및 엽장(葉長)은 파종후(播種後) 6개월(個月) 수확(收穫)부터 24개월(個月) 수확(收穫)까지 늦게 수확(收穫)할 수록 초장(草長)은 $0.7{\sim}6.5cm$엽장(葉長)은 $0.6{\sim}2.6cm$ 각각(各各) 길었으며, 특히 파종후(播種後) 18개월(個月) 이후(以後)는 신장정도(伸長程度)가 둔화(鈍化)되었다. 2. 수확시기별(收穫時期別) 입묘주수(立苗株數)는 파종당시(播種當時) $m^2$당(當) 주수(株數)가 78개(個)이었으나 6개월(個月) 후(後)에는 2.3배(倍), 12개월(個月) 후(後)에는 5.6배(倍), 18개월(個月) 후(後)에는 13.3배(倍), 24개월(個月) 후(後)에는 20.0배(倍)로 수확(收穫)을 늦게할 수록 크게 증가(增加)하였다. 3. 수확시기별(收穫時期別) 괴경장(塊莖長), 괴경폭(塊莖幅) 및 괴경량(塊莖量)은 파종후(播種後) 6개월(個月) 수확(收穫)${\sim}$12개월(個月) 수확(收穫) 까지는 증가폭(增加幅)이 미미(微微) 하다가 18개월(個月) 수확(收穫) 때 크게 증가(增加)하였으며 그 이후(以後)는 완만(緩慢)하게 증가(增加)하였다. 4. 수확시기별(收穫時期別) 괴경(塊莖)크기 분포(分布)는 파종후(播種後) 6개월(個月), 12개월(個月) 수확(收穫)은 중(中), 소괴경(小塊莖)이 많은 반면 18개월(個月) 이후(以後) 수확(收穫)은 상품성(商品性) 있는(직경(直徑) 7.1mm이상(以上)) 중(中), 대괴경분포(大塊莖分布)가 많았다. 5. 수확시기별(收穫時期別) 10a당(當) 생체수량(生體收量)은 파종후(播種後) 6개월(個月) 수확(收穫)은 172kg, 12개월(個月) 수확(收穫)은 231kg, 18개월(個月) 수확(收穫)은 345kg, 18개월(個月) 이후(以後) 수확(收穫)은 392kg으로 증수(增收)하였으나 24개월(個月) 증가폭(增加幅)은 미미(微微)하였다. 6. 지상부(地上部) 엽형(葉型)의 전개정도(展開程度)를 보고 지상부(地上部)의 괴경폭(塊莖幅)과 괴경량(塊莖重) 등(等)을 추정(推定)할 수 있었다. This study was carried out to determine optimum harvest time of in vitro multiplied tuber Pinellia ternata(Thunb.)Breit from 1990 to 1992. Tuber yield and its related traits were observed with four harvest times. Plant height was 7.2cm at 6 months and 19.3cm at 24 months after planting. The number of tubers per unit area was significantly increased with the passage of growing time after planting$(78\;tubers/m^2)$, those were increased by 2.3 times in 6 months, 5.6 times in 12 months, 13.3 times in 18 months and 20.0 times in 24 months. The tuber sizes(length, width and weight) were highly enlarged during 12 to 18 months after planting compared with before or after this time. There were smaller tubers and lower tuber yields with shorter growing period, while large tubers above 7.1mm and higher yields with longer growing. It was suggested that the optimum harvest time was 18 months after planting, before over wintering rather than next year of spring, 24months after planting.

기내 대량 생산 반하 종구의 포장 재배기술 연구

박문수(Moon Soo Park),박호기(Ho Ki Park),김태수(Tai Soo Kim),장영선(Yeong Sung Jang),박근용(Keun Yong Park) 한국약용작물학회 1993 한국약용작물학회지 Vol.1 No.2

This study was carried out to determine optimum harvest time of in vitro multiplied tuber Pinellia ternata(Thunb.) Breit from 1990 to 1992. Tuber yield and its related traits were observed with four harvest times. Plant height was 7.2cm at 6 months and 19.3cm at 24 months after planting. The number of tubers per unit area was significantly increased with the passage of growing time after planting (78 tubers/㎡), those were increased by 2.3 times in 6 months, 5.6 times in 12 months, 13.3 times in 18 months and 20.0 times in 24 months. The tuber sizes(length, width and weight) were highly enlarged during 12 to 18 months after planting compared with before or after this time. There were smaller tubers and lower tuber yields with shorter growing period, while large tubers above 7.1㎜ and higher yields with longer growing. It was suggested that the optimum harvest time was 18 months after planting, before over wintering rather than next year of spring, 24months after planting.

오갈피屬 植物의 形態 및 解剖學的 特性 比較

Young Jin Kim(金永珍),Ho Ki Park(朴昊基),Moon Soo Park(朴文洙),Sun Kim(金先),Kyeong Gu Choi(崔京求) 한국육종학회 1997 한국육종학회지 Vol.29 No.1

Morphological and anatomical characteristics of five Eleutherococcus species which are the important source for oriental medicine were investigated. The leaf and the spine shapes in morphology were the main factors to identify the Eleutherococcus species. The numbers and length of spine on the stem were prominent characters to identify the E. senticosus. In anatomical characters, the thickness of cork layer, phloem and xylem were different among the species. E senticosus is very similar with E. chiisanensis in their morphology but they could be distinguished by anatomical characters such as thickness of cork, secondary phloem and number of cell layer of cork in one year old branch. It was suggested that the ratio of secondary xylem thickness to radius of stem can be used as the measure related with production of useful substance in Eleutherococcus spp.

기능성 식품 자원의 지질, 아미노산 및 식이 섬유의 조성 - 길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 -

황성원(Sung-Won Hwang),박무희(Moo-Hee Park),심호기(Ho-Ki Shim),배만종(Man-Jong Bae) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4

길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 등의 식이 섬유소, 지방산 조성 및 염용성 단백질의 아미노산을 분석 비교한 결과는 다음과 같다. 식이 섬유로서 neutral detergent fiber(N.D.F), acid detergent fiber(A.D.F), lignin, hemicellulose, cellulose 등의 함량은 달맞이꽃 종자가 cellulose를 제외하고는 많은 함량이었다. 총지질의 지방산조성에서 P/S 비율은 들깨 종자가 6.31로 높았으며, linolenic acid (n-3)는 들깨 종자에 있어 55.47%, linoleic acid (n-6)는 달맞이꽃 종자에 71.88%로 그 함유비가 높았다. 중성지질의 지방산 조성은 총지질의 지방산 조성과 비슷하였다. 당지질의 지방산 조성에서 PUFA의 함유비는 들깨 종자 61.76%, n-6/n-3 비율은 달맞이꽃 종자가 15.19이었다. 인지질의 지방산 조성은 당지질의 지방산과 조성과 비슷하며, 길경의 PUFA의 함량은 62.96%로 약간 증가되었다. 염용성 단백질의 아미노산 조성에서 필수아미노산은 길경이 47 ㏖e%로 높은 함유비를 나타내었으며, E/N 비율은 길경이 0.9, 알로에 베라가 0.66이었다. This study was conducted to investigate the contents of dietary fiber(DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food sources such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F), acid detergent fiber (A.D.F), lignin, hemicellulose and cellulose in evening primrose seed were higher than those of other samples, except the content of cellulose. The ratio of polyunsaturated/saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88%, which was higher than those of other samples. The fatty acid compositions in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the same as those of glycolipids. The contents of PUFA in Platycodi radix were 62.96%. The essential amino acid contents of salt-soluble protein were 47mole% in Platycodi radix, which was slightly higher than those other samples. The ratio of essential amino acid / nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.

나물용 콩의 재배시기에 따른 수분흡수율과 발아율

김학신,김홍식,김경호,오영진,서석기,박호기,Kim Hag-Sin,Kim Hong-Sik,Kim Kyong-Ho,Oh Yeong-Jin,Suh Suk-Kee,Park Ho-Ki 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1

나물용 콩 6품종의 파종기에 따른 콩나물 원료콩의 수분흡수율과 발아율을 조사한 결과는 다음과 같다. 종실 수분흡수율은 파종기간에 차이가 없었으며 품종간에는 한남콩은 높았으나 다른 품종들은 큰 차이가 없었다. 발아율은 5월 25일 파종보다 늦어질수록 높았고, 품종간에는 풍산나물콩, 은하콩, 도레미콩과 소명콩이 치상 2일 후 $90\%$이상 이었으며 다원콩과 한남콩은 낮았다. 일반적으로 종자의 발아율 검정은 치상 후 7일간 조사를 실시한다. 그러나 콩나물 원료콩의 발아율 검정은 $25{\pm}1^{\circ}C$에서 3일간의 발아율로 함이 적합하다 하겠다. The water absorption ratio was not affected by the planting dates. Hannamkong showed higher water absorption ratio than any sprout -soybean varieties. Soybean seeds produced from later planting showed higher germination percentage than those planted May 25. Pungsannamulkong, Eunhakong, Doremikong, and Someongkong showed over $90\%$ germination after two days, while Tawonkong and Hannamkong were lower, The germination ratio test of the soybean seeds at $25^{\circ}C$ is usually for 7 days. But, germination ratio test of seeds used for sprout-soybean is suitable for 3 days at $25^{\circ}C$.

노화수분, NaCl 및 CO₂ 처리가 황기의 자가불화합성 타파에 미치는 영향

김영국(Young Guk Kim),김동휘(Dong Hwi Kim),박춘근(Chun Geon Park),여준환(Jun Hwan Yeo),안영섭( Young Sup Ahn),박호기(Ho Ki Park) 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.5

황기의 자가불화합성 타파를 위한 노화수분 처리, NaCl 처리 및 CO₂ 처리에 대한 결과를 요약하면 다음과 같다. 비닐하우스에서 노화수분은 8월 하순에는 거의 수정이 되지 않았으며, 9월 상순부터 10월 상순까지는 개화 당일 부터 개화 후 2일까지 수정되었고, 9월 하순 개화 당일 수분한 것이 결협율 33.3%, 결실율 86.2%로 가장 양호하였다. 노지에서 노화수분은 대조구는 8월 하순부터 10월 상순까지 결협은 되었으나, 9월 중순과 9월 하순을 제외하고 결실이 되지 않았으며, 9월 하순의 개화 후 1일에 결협율 39%, 결실율 94.9%로 가장 양호하였다. NaCl 처리는 비닐하우스에서는 1% 농도 처리가 결협율은 10월에서 21.3%로 가장 좋았으나, 결실율은 9월 하순 66.7%로 가장 양호 하였고, 노지에서는 NaCl 농도 5%에서 결협율 7.3%, 결실율 90.9%로 가장 양호하였다. 식물생육상을 이용하여 CO₂ 700 ppm을 처리한 결과 무처리구와 큰 차이 없었다. 결론적으로 황기 자가불화합성 타파를 위하여 9월 중하순에 자화가 아닌 자가화를 개화당일 또는 개화 후 1일에 인공수분해주거나 NaCl 1~5%를 처리해주면 자가불화합성을 타파할 수 있다고 사료된다. This study was conducted to break down the self-incompatibility of Astragalus membranaceus Bunge by old-flower pollination, NaCl and CO₂ treatment. The old-flower pollination in green house produced fertilization during the 1st and 2nd day after flowering from early September to early October, but almost no fertilization in late August. The most successful pollination occurred in late September at the 1st day after flowering when pod setting was 33.3% and that of seed set was about 86.2%. The old-flower pollination in field showed pod setting for the control group from late August to early October, but no seed set except the days of mid-September and late September. The most successful pollination occurred in late September during the 2nd day of flowering when the percentage of pod setting was 39% and that of seed set was 94.9%. The wrapping in field set pods from late August to early October, but did not set seeds except in mid and end of September. The percentage of pods was 39% and percentage of seeds was 94.9% about flowering after first day in end of September. by field culture. The best result from NaCl treatment was achieved when 1% NaCl treatment in green house produced 21.3% of pod setting in early October and 66.7% of seed set in late September. In field, NaCl 5% treatment produced best result with 7.3% of pod setting and 90.9% of seed set in mid-September. No differences were observed between the CO₂ 700ppm treatment and the control group.

엽록체 형질전환기술 및 콩 벡터개발

김영진 ( Young Jin Kim ),이승범 ( Seung Bum Lee ),엄미옥 ( Mi Ok Um ),심형권 ( Hyeong Kwon Shim ),박호기 ( Ho Ki Park ),( Henry Daniell ) 한국콩연구회 2005 韓國콩硏究會誌 Vol.22 No.1