Immune Response of Alpha-toxin, Capsular Polysaccharide (CPS) and Recombinant Fibronectin-Binding Protein (r-FnBP) of Staphylococcus aureus in Rabbit

박희명,유한상,한흥율,김두,Park, Hee-Myung,Yoo, Han-Sang,Han, Hong-Ryul,Kim, Doo The Korean Society of Veterinary Clinics 1998 한국임상수의학회지 Vol.15 No.2

본 연구는 황색포도상구균의 병원성 인자인 alpha-toxin, casular polysaccharides (CPS)와 fibronectin-binding protein (FnBP)을 이용해 실험동물인 토끼에서의 항체가 형성 능과 공격접종 후 방어능에 관한 연구를 위하여 수행되었으며 향후 젖소 유방염 아단위 항원 을 이용한 백신개발 가능성을 탐색하고자 수행되어졌다. 황색포도상구균의 alpha-toxin, capsu18r polysaccharides (CPS)91 fibronectin-binding protein (FnBP) 항원을 이통재 효소면 역측정법을 통한 혈중 IgG 항체가수준을 측정하였으며 alpha-toxin, capsular polysaccharides (CPS)와 fibronectin-binding protein (FnBP)으로 면역시킨 토끼에서 대조군의 토끼보다 혈 중 항체가 수준이 1차 면역 이후 유의성 있게 높았다 (p<0.05). 백신에 사용된 alpha-toxin, capsular polysaccharides (CPS)와 fibronectin-binding protein (FnBP) 항원중 capsular polysaccharides (CPS)가 다른 alpha toxin과 fibronectin-binding protein (FnBP)의 혈중항 체가 수준과 비교하여 볼 때 비교적 낮은 수준이었다. 세균을 혈중으로 공격접종한 후 대조군 과 백신접종군의 혈중내 세균제거율에 있어 대조군에 비해 백신접종군에서 유의성 있게 낮은 균수를 보였다 (p<0.05). 또한 장기내 균수측정실험결과 대조군의 장기보다 백신접종군에서 유의성 있게 세균수가 낮게 출현하였다 (p<0.05). 결론적으로 본 연구결과 황색포도상구균의 3가지 항훤 alpha-toxin, capsular polysaccharide와 재조합 fibronectin binding protein을 이 퐁한 실험동물에서 안단위 유방염 백신은 방어능이 있다고 생각된다.

반려동물에서 항생제 내성 관리

박희명 ( Hee-myung Park ) 대한인수공통감염병학회 2023 창립총회 및 학술대회 초록집 Vol.2023 No.2

갑상선암 환자의 방사선옥소 외래치료시 가족 구성원의 방사선량 측정

박희명 ( Hee Myung Park ),장정웅 ( Jung Woong Jang ),양희철 ( Hee Chul Yang ),김영국 ( Young Gook Kim ) 대한핵의학회 2007 핵의학 분자영상 Vol.41 No.3

반려동물 기능성 제품 시장현황

박희명(Hee-Myung Park) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

Metaraminol 및 Phenoxybenzamine 이 출혈성 저혈압증의 신기능에 미치는 영향에 관한 연구

박희명 ( Hi Myung Park ),이희석 ( Hee Suk Lee ) 대한내과학회 1971 대한내과학회지 Vol.14 No.6

A comparative study was made on the renal effects of metaraminol, a sympathomimetic agent, and phenoxybenzamine, a sympathetic blocking agent, when given in acute hemorrhagic hypotension in dogs induced by the arterial bleeding to the amount of 15ml/kg. Fo

강아지에서 컨벡스스캐너를 이용한 정상적인 심장구조물의 초음파상

정순욱,박희명,한숙희,윤정희,한홍율,Jeong Soon-wuk,Park Hee-myung,Han Sook-hee,Yoon Jung-hee,Han Hong-ryul 한국임상수의학회 1994 한국임상수의학회지 Vol.11 No.2

Sector scanner which has a conical end is used to image through the intercostal space because heart is protected by the ribs. Cardiac data published all around the world were also obtained by sector scanner. Although scanners being used in every small animal practice and animal hospital at college in Korea include convex ape and linear type, linear type is not appropriate f3r cardiac scan because of a wide contact surface. The purpose of this study is to establish ultrasonographic images of normal cardiac structures by measuring shape, size of reflectable cardiac structure according to restraint position in scanning normal heart of the puppies with 6.5 MHz convex scanner(SonoAce 4500, Medison, Korea) used in our veterinary teaching hospital, Seoul national university. Seventeen male and female puppies considered having healthy hear by X-ray and clinical examination are used feom April to July 1994. Scanning point selection of probe head and the distinction of imaged cardiac structures were accomplished by necropsy and cardiac scanning performed through thoracotomy under general anesthesia. At 10 o'clock position of transducer(at an angle of 30$^{\circ}$ between imaginary line from elbow joint to 3rd sternum and probe head, 60$^{\circ}$ from body surface, 4th intercostal space of right thorax) with the marker of scanner toward the head of dogs right atrium, left atrium and left ventricle were observed in 2, 3, 4, 5 intercostal space(2cm from the sternum) of experimental dog positioned ventrodorsally under general anesthesia. Under these conditions, the numerical values of imaged diastolic hear are as follows : the distance from skin to apex(mean$\pm$S.D) 47.53$\pm$6.94mm, thickness of left ventricular wall 6.00$\pm$1.60mm, length of left ventricle 16.27$\pm$5.31mm, width of left ventricle 15,33$\pm$4.25mm, length of left atrium 12.33$\pm$3.82mm, width of left atrium 11. 33$\pm$3.94mm, length of right atrium 1.00$\pm$2.41mm, width of right atrium 11.21$\pm$2.76mm and the area of left ventricle 270.92$\pm$109.81mm$^2$, area of left atrium 98.00$\pm$41.08mm$^2$, area of right atrium 62.75$\pm$21.04mm$^2$.

재혼노인의 결혼만족도에 영향을 미치는 요인에 관한 연구 : 전라남도를 중심으로

박일연(Park Yill Youn),노길희(Noh Gil Hee),박희명(Bang Hee Myung) The Research and Information Service 2005 남부대학교 논문집 Vol.5 No.-

This study aims to establish interventional social work plans that can support remarried elderly people by analyzing factors affecting their level of satisfaction with their marital. Towards this end, a research project with 209 elderly people in Jeonnam Province, who remarried after they turned 60 or older, was conducted to determine the characteristics of their remarriage, issues between the spouses and their social circumstances before and after remarrying, and to analyze how these factors affect their level of satisfaction with Marital. Based on the results of the analysis, the study came up with suggestions for social work intervention. The results of this study on the factors affecting marital satisfaction of the remarried elderly were as follows : First, the frequency of de facto marriage rather than a law marriage was high. Second, their responses when asked about attitudes toward their remarriage were negative Third, all of the participants in this study responded that the level of the communication was relatively great, but the couples' participation in social activities was extremely poor. Fourth, The higher the level of scholarship and social supportive, couple communication, the higher the level of marital satisfaction. Fifth, As for clinical suggestions, the study recommends that the following projects be undertaken: providing self group activity programs in order to improve their level of communication and social supportive. Finally, The study further suggests that future studies are needed, for example to examine the differences between the husband and the wife by including couples as subjects 본 연구는 전라남도에 거주하는 노인 중 만 60세 이후에 재혼한 노인 209 명 의 결혼만족도에 영향을 미치는 요인에 대하여 조사하였다. 조사기간은 2003년 8월 18일부터 9월 6일까지 각 동면 사무소 사회복지 전담 공무원을 통하여 조사를 실시하였다. 조사내용은 재혼관련특성, 부부관계 특성, 사회관계 특성, 결혼만족 실태를 파악하였다. 이를 통해 다음과 같은 결론을 얻을 수 있었다. 첫째, 법률혼보다도 사실혼관계가 높게 나타나 사실혼 관계가 노인재혼의 보편적인 현상임을 알 수 있었다. 둘째, 자녀의 재혼태도가 비교적 부정적이었다. 셋째, 재혼노인의 부부관계 특성인 부부의사소통은 비교적 높았으나, 사회 활동 참여도가 낮게 나타났다. 넷째, 화력이 높고, 사회적 지지가 높으며, 부부의사소통정도가 높을수록 결혼만족도가 높게 나타났다. 다섯째, 사실혼관계에 있고, 경제적으로 열악하며, 자녀의 지지가 부족한 재혼노인들을 위한 자조집단 프로그햄의 개발과 지원이 필요하다. 일곱째, 재혼노인 부부 및 가족들의 재혼에 관련한 의식조사가 향후 필요하다.

서울지역에서 문제되고 있는 강아지의 급성설사증에 관한 병인학적 연구

한홍율,박희명,이진희,오태호,정순욱,윤신근,박철만,Han Hong-Ryul,Park Hee-Myung,Lee Jin-Hee,Oh Tae-Ho,Jeong Soon-Wuk,Youn Sin-Keun,Park Cheol-Man 한국임상수의학회 1993 한국임상수의학회지 Vol.10 No.2

In Seoul area, there are so many kennel clubs, veterinary hospital, pet establishment and breeding confers that various problems have occurred. They are crowed pet houses, poor sanitation, stress to puppies, sudden environmental changes to puppies and unvaccination against parvovirus, canine distemper virus, parainfluenza virus, infectious hepatitis caused by Adenovirus type I and Leptospira. Several studies were made to survey the infectious agents involved in acute diarrhea of poppies in Seoul are, such as history taking, physical examination, complete blood count and serum chemistry, histopathological finding, bacterial isolation and identification, and hemagglutination test in feces and hemagglutination inhibition test in serum against parvovirus, respectively. The results obtained are summarized as followed. 1. The percent of PCV (30.5$\pm$5.6) and concentration of Total protein(5.0$\pm$0.8) resulted from statistical analysis are significantly lower than normal values (p<0.05), respectively. In addition, fibrinogen (505$\pm$326) was significantly higher an normal value (p<0.001), Band neutrophil (22.9$\pm$12.7) showed signifiant difference (P<0.01). decreased monocyte (3.2$\pm$2.1) and eosinophil (0.7$\pm$0.8) values appeared statistically significant (p<0.001), lymphocyte (16.7$\pm$9), as well. 2. The concentrations of calcium .(8.0$\pm$2.8), glucose (40.1$\pm$31.4), and albumin (2.0$\pm$0.39) were lower than normal values (p<0.01, p<0.01, p<0.001), respectively. Also Inorganic phosphate (7.1$\pm$2.4), pH (p<7.9f:0.2), and Blood Urea Nitrogen (40.41=37.1) were significantly higher than normal values (p<0.001, p(0.001, p<0.05), respectively. 3. Simple and mixed infections occupied 18% and 82% in the distribution of causes in puppies with acute diarrhea, respectively. 4. As puppies got older, incidence of acute diarrhea caused by Staphylococcus aureus was decreased to 13% and infection of canine distemper virus was increased to 53%, but E coli and canine parvovirus always showed high frequency of outbreak in the body weight ranged from 35g to 7.8Kg. 5. As showed in table 5, infections of E coli and Canine Parvovirus showed high outbreak regardless of the age which is classified into three stages, 35~50 day, 60 day and 75~day to 1 year, canine distemper virus appeared increased, but in case of Staphylococcus aureus, visa versa. 6. In comparison wi methods for the laboratory diagnosis diagnosis parvovirus, Hemagglutination test showed positive reaction in 25% and mean serum antibody titer measured by Hemagglutination inhibition test showed 2779 (n=20). In addition, positive reaction was 90% (18/20). 7. In histopathological studies, enteritic and pneumonic lesions were indicated in 53.7% and 39.5% of samples, respectively. 8. Etiologic diagnosis based on the history taking, clinical signs and histopathological findings in puppies with acute diarrhea and vomiting indicated that 50% of puppies were infected by canine parvovirus and distemper virus. 9. In the parasitological examination made by simple flotation with saturated zinc sulfate tour parasites found were Isospora canis, Toxocara canis, Ancylostoma spp and Toxocara spp. Isospora canis and Toxocara canis were more frequently found among those parasites of diarrhoeic causes in puppies ranged from 35 days to 1 year. Their infestation rates were 15% and 13% respectively.

젖소 목장에서 분리된 황색포도상구균의 아형 분포와 특성

유종현,박희명,오태호,손대호,한홍율,Yoo, Jong-hyun,Park, Hee-myung,Oh, Tae-ho,Sohn, Dae-ho,Han, Hong-ryul 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.5

Staphylococcus aureus is one of most prevalent intramammary pathogens and have characteristics which are not easily eradicated. Recently, to understand the sources and transmission of S aureus, many studies have focused on the subtyping of field isolate. This study was preformed to investigate the distribution pattern and characteristics of the isolates using phenotyping and genotyping. Samples were collected from milk of each udder, cow bodies (perianal region, vagina, tail, udder skin, sole) and environment (floor, liner, milker's hands, water, towel, insect) from 6 herds located in Kyung-gi province. Forty five strains of S aureus were isolated from 3 dairy herds (A, B, C) and were typed by hemolytic pattern, antibiotic resistant pattern, enterotoxin typing and PCR-based DNA fingerprinting. Slime productivity was also compared by each subtype to examine potential infectiousness. Of 45 strains, 41 were isolated from milk samples and 4 were isolated from liners. No strains isolated in the bodies and environment. Forty five strains isolated were classified as 18 subtypes by phenotyping and genotyping. There was common subtype between A and B herd, but the subtype of C herd showed different pattern. Among predominant subtypes, 60% of S aureus strain isolated from A and B herd showed subtype I and 50% of S aureus strain isolated from C herd belonged to subtype VI and X II. Neither somatic cell count (SCC) nor slime production was significantly different between predominant and minor subtypes. In summary, the study revealed that liners play more important roles in the mode of transmission than environmental sources. Several subtypes can be found in a herd, only a few subtype, however, was largely associated with the majority of infection.

진도견에서 발생한 후천성 갑상선 기능저하증

오태호,박희명,Oh, Tae-ho,Park, Hee-myung 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.5

A four-year-old female Jindo dog was admitted to the hospital because of bilaterally symmetrical alopecia and inappentence. Systemic abnomalities detected on physical examination were obesity, bradycardia, exercise intolerance, mental dullness and hypothermia. Dermatologically symmetrical alopecia on the back, trunk and dorsal tail, and ventral hyperpigmentation were observed. Also face was very tragic. The level of basal $T_4$ was 0.01ug/dl. In TSH stimulation test $fT_4$ value was 0.08ng/dl. After treatment was initiated with L-thyroxine the dog's general condition was improved at 4 weeks.