산복사나무(Prunus davidiana (Carriere) Franch.) 열매 분획 추출물의 항산화 및 미백 효능

김원백 ( Won Baek Kim ),박소혜 ( So Hae Park ),황혜선 ( Hye Sun Hwang ),우정윤 ( Jung Yoon Woo ),이혜련 ( Hye Ryun Lee ),황대연 ( Dae Youn Hwang ),최지혜 ( Ji Hye Choi ),이희섭 ( Hee Seob Lee ) 부산대학교 김치연구소 2012 김치의 과학과 기술 Vol.15 No.-

본 연구에서는 산복사나무 열매 과육부를 80% 메탄올 추출물과 이를 극성과 비극성에 따른 순차적 분획물에 대하여 DPPH 소거능, nitrite 소거능, 총항산화능 및 총플라보노이드, 총폴리페놀 함량을 측정하고 tyrosinase 저해활성을 측정하였다. 그 결과 DPPH 소거능의 값은 에틸아세테이트 분획과 부탄올 분획이 5.8±0.1μg/mL, 8.1±0.2μg/mL 로 높은 활성을 나타내었으며, nitrite 소거능 역시 에틸아세테이트 분획과 부탄올 분획의 IC50 값이 88.1±0.9μg/mL, 134.8±1.0±로 높은 활성을 나타내었다. 총항산화능(TEAC)은 에틸아세테이트 분획이 6.21±0.01 mM로 높은 활성을 나타내었으며 ascorbic acid, BHA와 유의적인 차이가 없는 항산화능을 보였다. 총플라보노이드와 총폴리페놀 함량 역시 에틸아세테이트 분획과 부탄올 분획에서 , 244.5±1.4, 138.1±0.3 mg/g과 210.2±3.9,, 198.5±1.9 mg/g으로 가장 높았으며, tyrosinase 저해활성의 IC50 값도 0.310±0.002, 0.329±0.006mg/mL로 에틸아세테이트 분획과 부탄올 분획의 활성이 높았다. 따라서 본 연구 결과 산복사나무 열매의 에틸아세테이트 분획물과 부탄올 분획은 높은 항산화 활성 및 tyrosinase 저해활성을 가지고 있으며 이는 천연항산화제 및 미백제로서 활용이 가능함을 시사하며 추가적인 연구를 통해 기능성 소재 개발에 유용한 자원으로 활용될 수 있을 것으로 판단된다. In this study, methanol extract and its organic solvent fractions were prepared from Prunus davidiana (Carriere) Franch. fruit. Antioxidative activities, polyphenolic and flavonoid contents, and tyrosinase inhibitory activities were evaluated. Among the fractions, ethyl acetate fraction showed the highest antioxidative activity with IC50 values of 5.8 μg/mL and 88.1 μg/mL for DPPH radical and nitrite scavenging, respectively, which were comparable to those of ascorbic acid. Further, total flavonoid and polyphenolic contents were highest in the ethyl acetate fraction with IC50 values of 244.5 mg/g and 210.2 mg/g, respectively. IC50 values for tyrosinase inhibitory activity were 0.310 mg/mL and 0.329 mg/mL for the ethyl acetate and n-butanol fractions, respectively. Based on these results, it is suggested that the ethyl acetate fraction of Prunus davidiana (Carriere) Franch. fruit could be used as a functional material in the food and pharmaceutical industries.

산복사나무(Prunus davidiana (Carriere) Franch.) 열매 분획 추출물의 항산화 및 미백 효능

김원백(Won Baek Kim),박소해(So Hae Park),황혜선(Hye Sun Hwang),우정윤(Jung Yoon Woo),이혜련(Hye Ryun Lee),황대연(Dae Youn Hwang),최지혜(Ji Hye Choi),이희섭(Heeseob Lee) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.10

본 연구에서는 산복사나무 열매 과육부를 80% 메탄올 추출물과 이를 극성과 비극성에 따른 순차적 분획물에 대하여 DPPH 소거능, nitrite 소거능, 총항산화능 및 총플라보노이드, 총폴리페놀 함량을 측정하고 tyrosinase 저해활성을 측정하였다. 그 결과 DPPH 소거능의 IC50 값은 에틸아세테이트 분획과 부탄올 분획이 5.8±0.1 μg/mL, 8.1±0.2 μg/mL로 높은 활성을 나타내었으며, nitrite 소거능 역시 에틸아세테이트 분획과 부탄올 분획의 IC50 값이 88.1±0.9 μg/mL, 134.8±1.0 μg/mL로 높은 활성을 나타내었다. 총항산화능(TEAC)은 에틸아세테이트 분획이 6.21±0.01 mM로 높은 활성을 나타내었으며 ascorbic acid, BHA와 유의적인 차이가 없는 항산화능을 보였다. 총플라보노이드와 총폴리페놀 함량 역시 에틸아세테이트 분획과 부탄올 분획에서 244.5±1.4, 138.1±0.3 mg/g과 210.2±3.9, 198.5±1.9 mg/g으로 가장 높았으며, tyrosinase 저해활성의 IC50 값도 0.310±0.002, 0.329±0.006 mg/mL로 에틸아세테이트 분획과 부탄올 분획의 활성이 높았다. 따라서 본 연구 결과 산복사나무 열매의 에틸 아세테이트 분획물과 부탄올 분획은 높은 항산화 활성 및 tyrosinase 저해활성을 가지고 있으며 이는 천연항산화제 및 미백제로서 활용이 가능함을 시사하며 추가적인 연구를 통해 기능성 소재 개발에 유용한 자원으로 활용될 수 있을 것으로 판단된다. In this study, methanol extract and its organic solvent fractions were prepared from Prunus davidiana (Carriere) Franch. fruit. Antioxidative activities, polyphenolic and flavonoid contents, and tyrosinase inhibitory activities were evaluated. Among the fractions, ethyl acetate fraction showed the highest antioxidative activity with IC50 values of 5.8 μg/mL and 88.1 μg/mL for DPPH radical and nitrite scavenging, respectively, which were comparable to those of ascorbic acid. Further, total flavonoid and polyphenolic contents were highest in the ethyl acetate fraction with IC50 values of 244.5 mg/g and 210.2 mg/g, respectively. IC50 values for tyrosinase inhibitory activity were 0.310 mg/mL and 0.329 mg/mL for the ethyl acetate and n-butanol fractions, respectively. Based on these results, it is suggested that the ethyl acetate fraction of Prunus davidiana (Carriere) Franch. fruit could be used as a functional material in the food and pharmaceutical industries.

γ-Secretase 활성억제단백질인 TMP21의 과발현이 신경세포주에서 NGF 수용체 신호전달과 정에 미치는 영향

Sun Il Choi(최선일),Seung Wan Jee(지승완),Youn Kyung Her(허윤경),Ji Eun Kim(김지은),So Hee Nam(남소희),In Sik Hwang(황인식),Hye Ryun Lee(이혜련),Jun Seo Goo(구준서),Young Ju Lee(이영주),Eon Pil Lee(이언필),Hae Wook Choi(최해욱),Hong 한국생명과학회 2011 생명과학회지 Vol.21 No.8

TMP21은 AD의 원인으로 작용하는 Aβ-42 펩타이드 생성에 중요한 γ-secretase 활성을 억제하는 p24 family에 속하는 type I 막 단백질이다. 본 연구에서는 TMP21이 세포의 성장과 분화에 중요한 NGF 수용체 신호전달과정에 미치는 영향을 분석하고자 인간의 TMP21 cDNA를 합성하고, CMV promoter 조절 하에 hTMP21를 클로닝하여, CMV/hTMP21 벡터를 제조하였다. 그리고 이들 벡터를 B35 neuroblastoma에서 과발현시킨 후 γ-secretase 구성단백질과 NGF 수용체 연관 단백질의 변화를 관찰하였다. 그 결과, 4종류의 γ-secretase 구성단백질의 발현은 vehicle transfectants보다 CMV/hTMP21 transfectants에서 유의적으로 감소하였다. 또한 NGF low affinity 수용체인 p75<SUP>NTR</SUP>과 downstream 단백질인 RhoA의 양은 NGF를 처리하지 않은 TMP21 transfectants에서 유의적으로 증가하였으나 NGF 처리에 의해 감소되었다. High affinity NGF 수용체인 TrkA의 인산화도 NGF 처리가 없는 경우 유의적으로 감소하였으나 NGF 처리에 의해 증가되었다. 또한 downstream 신호전달 과정 중에서 ERK의 인산화는 TrkA와 유사한 발현변화를 나타내었으나 Akt 인산화는 NGF의 처리에 의해 더욱 증가하였다. 이러한 결과는 TMP21이 neuroblastoma에서 NGF 수용체 신호전달과정를 조절하는 중요한 단백질로서 작용함을 제시하며, AD의 작용기전 연구에 중요한 기초자료를 제공할 것으로 사료된다. Transmembrane protein 21 (TMP21) is a member of the p24 cargo protein family and has been shown to modulate γ-secretase-mediated Aβ production which was specifically observed in the brains of subjects with Alzheimer’s disease (AD). In order to investigate whether TMP21 could affect nerve growth factor (NGF) receptor signaling pathway, the alteration of NGF receptors and their downstream proteins were detected in TMP21 over-expressed cells. CMV/hTMP21 vector used in this study was successfully expressed into TMP21 proteins in B35 cells after lipofectamin transfection. Expressed TMP21 proteins induced the down-regulation of γ-secretase complex components including Presenlin-1 (PS-1), PS-2, Nicastrin (NST), Pen-2 and APH-1. Also, the expression level of NGF receptor p75<SUP>NTR</SUP> and RhoA were significantly higher in CMV/hTMP21 transfectants than vehicle transfectants, while their levels returned to vehicle levels after NGF treatment. However, the phosphorylation of NGF receptor TrkA was dramtically decreased in NGF No-treated CMV/hTMP21 transfectants compared with vehicle transfectants, and increased in NGF treated CMV/hTMP21 transfectants. In TrkA downstream signaling pathway, the phosphorylation level of ERK was also decreased in CMV/hTMP21 transfectants, while the phosphorylation of Akt was increased in the same transfectants. Furthermore, NGF treatment induced the increase of phosphorylation level of Akt and ERK in CMV/hTMP21 transfectants. Therefore, these results suggested that over-expression of TMP21 may simultaneously induce the up-regulation of p75<SUP>NTR</SUP>/RhoA expression and the down-regulation of TrkA/ERK phosphorylation through the inhibition of γ-secretase activity.

LP9M80-H Isolated from Liriope platyphylla Could Help Alleviate Diabetic Symptoms via the Regulation of Glucose and Lipid Concentration

Ji Eun Kim(김지은),In Sik Hwang(황인식),Jun Seo Goo(구준서),So Hee Nam(남소희),Sun Il Choi(최선일),Hae Ryun Lee(이혜련),Young Ju Lee(이영주),Yoon Han Kim(김윤환),Se Jin Park(박세진),Nahm-Su Kim(김남수),Young Hwan Choi(최영환),Dae You 한국생명과학회 2012 생명과학회지 Vol.22 No.5

LP9M80-H는 맥문동(Liriope platyphylla)으로부터 메탄올과 헥산을 이용하여 추출한 새로운 추출물로서 ICR 마우스에서 인슐린분비를 촉진하며, 간과 뇌 조직에서 인슐린 신호경로를 활성화시키는 것으로 알려져 있다. 본 연구에 서는 LP9M80-H가 당뇨와 비만의 치료에 미치는 효과를 조사하기 위하여, OLETF 모델동물에 LP9M80-H를 2주간 투여한 후 당뇨와 비만과 관련된 주요인자의 변화를 관찰하였다. 비록 체중은 두 집단간에 차이가 없었으나 복부 지방량은 vehicle 투여군보다 LP9M80-H 투여군에서 적었다. 또한, 혈중 포도당농도는 LP9M80-H를 투여한 OLETF랫드가 대조군에 비하여 약간 낮았으나 인슐린의 농도는 유의적으로 크게 증가하였다. 혈청 내 3가지 주요 지질의 농도는 LP9M80-H를 투여한 OLETF 랫드에서 유의적으로 감소하였고, 지방의 산화를 촉진하는 아디포넥틴의 농도도 LP9M80-H를 투여한 OLETF 랫드에서 감소하였다. 더불어, 체내에 분비된 인슐린이 표적장기에 미치는 영향을 관찰하기 위하여 간조직에서 인슐린 수용체와 인슐린 수용체기질(IRS)의 발현을 관찰하였으며, 이러한 2가지 단백질은 LP9M80-H를 투여한 OLETF 랫드에서 vehicle 투여군에 비해 유의미하게 감소하였다. 또한, 인슐린 신호경로의 다운스트림에 위치하는 포도당 수송체 중에서 Glut-2와 Glut-3 발현은 LP9M80-H를 투여한 OLETF 랫드에서 유의미하게 감소하는 반면에, Glut-4 발현은 일정하게 유지되었다. 따라서 이러한 결과는 LP9M80-H는 포도당항상성과 지질농도의 조절을 통하여 당뇨와 비만의 증상을 완화시키는데 기여할 것으로 사료된다. It was reported that the novel compounds (LP9M80-H) of Liriope platyphylla regulate glucose transporter (Glut) biosynthesis by activating the insulin-signaling pathway in the liver and brain of ICR mice. To investigate the therapeutic effects of LP9M80-H on the pathology of diabetes and obesity, alterations of key factors related to symptoms were analyzed in the Otsuka Long Evans Tokushima Fatty (OLETF) rats treated with LP9M80-H for 2 weeks. The abdominal fat masses in the LP9M80-H-treated group were lower than the vehicle-treated group, although there was no difference in body weight between the two groups. Additionally, when compared to the vehicle-treated group, LP9M80-H treatment induced a significant decrease in glucose levels and an increase in the insulin concentration in the blood of OLETF rats. A high level of insulin protein was also detected in pancreatic β cells of LP9M80-H-treated OLETF rats. A significant reduction in the concentration of lipids and adiponectin was detected only in LP9M80-H-treated OLETF rats. Furthermore, the expression of insulin receptor β and the insulin receptor substrate (IRS) was dramatically decreased in LP9M80-H-treated OLETF rats compared to the vehicle-treated group. Of the glucose transporters located downstream of the insulin-signaling pathway, glucose transporters (Glut) -2 and -3 were significantly decreased in LP9M80-H-treated OLETF rats, while the level of Glut-4 was maintained under all conditions. Therefore, these results suggest that LP9M80-H may contribute to relieving symptoms of diabetes and obesity through glucose homeostasis and regulation of lipid concentration.

부모의 학대가 청소년의 적응에 미치는 영향

이혜련,최보가 한국청소년복지학회 1999 청소년복지연구 Vol.1 No.1

This Study examined parental abuse and sought to determine if any difference in type were gender related ; it also sought to determine the influence of parental abuse on adolescent adjustment. The sample consisted of 808 adolescents living in Daegu. The findings of this study were as follows : (1) There was a difference in parental behavior. Mothers were more emotionally abusive than fathers - but fathers were more neglectful than mothers. (2) Parental abuse had effects on adolescen t adjustment. Fathers of male adolescents had the greatest effects on the adjustment of their sons ; similary, mothers were the strongest influence in determining the adjustment of daughters.

학령전 아동의 정서이해와 부모의 정서표현성 및 아동정서 수용태도와의 관계

최보가,이혜련 대한가정학회 2002 Human Ecology Research(HER) Vol.40 No.10

This study investigated the relation between preschoolers' emotion understanding and parents' emotion expressiveness and attitude toward children's emotion expressiveness. Subjects were ninety 3- to 5-year old children and their parents. Parents' emotion socialization was measured by PACES developed by Saami(1989) and FEQ developed by Harberstadt(1986). And preschoolers' identification of basic emotional expressions and expression of their own feelings and others' feelings in various situations were measured. Results revealed that 5-year-old children understood emotion better than 3-year-old children, and mother's positive emotion expression influenced children's emotion understanding. The results are consistent with recent research showing that parents emotion socialization may be important for preschoolers' emotion understanding.

청년의 주관적 건강인지와 음주동기 및 문제음주에 관한 연구

백성희,김혜영,주민선,배정은,이혜련 이화여자대학교 간호과학연구소 2002 Health & Nursing Vol.14 No.1

Objective : The aim of the present study was to investigate the subjective health, drinking motives and drinking problems in young adults. Materials and Methods : The Data were collected through the structured questionnaire that including general characteristics, subjective health, four drinking motives and drinking problems survey targeting the 299 young adults from April 2002 to May 2002. In the first place, the present study tested a four-factor model of drinking motives recently proposed by Cox and Klinger (1988, 1990). Results : The prevalence of drinking problems was 36.5%, obstainers comparised 63.5% of the sample. Hazardous drinkers represented 26.4% and alcohol dependence 3.7% of the drinking problems. Through the multiple regression analyses, the effects of subjective health, drinking motives on drinking problems were examined. Results showed that drinking motives were found to have significant effects on drinking problems. Conformity motives, enhancement motives, social motives, coping motives, for example, influenced upon drinking problems indirectly through alcohol use, It was particularly noted that conformity motives, enhancement motives, coping motives and social motives were the strongest predictor in explaining the Hazardous drinking and alcohol dependence of drinking problems. While subjective health had only adjusted on drinking problems. Conclusions : In conclusion, the present study could work as a stepping stone for guiding the directions of further research. So efforts to prevent drinking problems in young adults can take many different drinking motives.