Glucocorticoid Regulation of Gene Expression in Hippocampal CA3 and Dentate Gyrus

김동섭,안순철,김영진,박병권,안용태,김지연,허송욱,Kim, Dong-Sub,Ahn, Soon-Cheol,Kim, Young-Jin,Park, Byoung-Keun,Ahn, Yong-Tae,Kim, Ji-Youn,Kyoji, Morita,Her, Song Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

글루코코르티코이드는 해마 조직에서 대사, 스냅신 형성, apoptosis, 신경세포 생성과 세포에 있어서 수지상의 형태에 영향을 준다. 글루코코르티코이드 호르몬에 의한 해마조직의 생리학적 조절을 이해하기 위하여, CA3와 DG (dentate gyrus)에서 유전자 발현에 대하여 조사하였다. Lewis 쥐에 9.5mg의 코르티코스테론 알약 또는 플라시보 알약을 20일 동안 처리한 후에 올리고머 유전자 칩을 이용하여 유전자 발현을 조사 하였다 (Rat Neurobiology U34 Arrays, Affymetrix). 플라시보 알약을 처리한 쥐에서 32 유전자들이 DG보다 CA3에서 발현이 높았으며, 3개 유전자는 CA3보다 DG에서 높은 발현을 보였다. 코르티코스테론 호르몬 처리에 의한 해마조직의 유전자 발현 형태는 해부학적 구조의 차이를 보였다. 특히, CA3에서 6개의 유전자와 DG에서 41 개의 유전자가 호르몬에 의하여 조절 받았으며, 이중 43개의 유전자가 상승 발현하였으며, 4개의 유전자가 하강 발현 하였다. 이들 유전자를 기능에 의해 분류하면, 13개의 신경전달물질관련 유전자, 5개의 이온채널,4개의 전사인자, 3개의 neurotrophic인자, 1개의 각 사이토카인과 apoptosis관련 유전자, 그리고 5개의 스냅신형성관련 유전자가 해마조직에서 발현의 변화를 보였다. 특히, 스트레스 호르몬에 의하여 CA3에서 BDNF의 감소를 볼 수 있었다. 이러한 결과는 호르몬에 의하여 해마구조의 생리학적인 다양성을 내포 하고 있다. Glucocorticoids (GCs) alter metabolism, synaptogenesis, apoptosis, neurogenesis, and dendritic morphology in the hippocampus. To better understand how glucocorticoids regulate these aspects of hippocampal biology, we studied gene expression patterns in the CA3 (Hippocampal pyramidal cell field CA3) and dentate gyrus (DG). Litter-matched Lewis inbred rats treated for 20 days with either 9.5 mg per day sustained-release corticosterone or placebo pellets were compared with high-density oligonucleotide microarray analysis (Rat Neurobiology U34 Arrays, Affymetrix). In placebo-treated rats, 32 genes were expressed at greater levels in CA3 than DG, whereas 3 genes were expressed at great levels in DC than CA3. Regional differences were also apparent in corticosterone-induced changes in the hippocampal transcriptome. Six genes in CA3 and 41 genes in DC were differentially regulated by corticosterone. As per the glucocorticoid effects on gene transcription in the brain, forty three of these genes were upregulated, and 4 genes were downregulated. Genes differentially expressed in hippocampus included those for 13 neurotransmitter proteins, 5 ion channel related proteins, 4 transcription factors, 3 neurotrophic factors, 1 cytokine, 1 apoptosis related protein, and 5 genes involved in synaptogenesis. Interestingly, GCs can have suppressive effects on brain BDNF mRNA transcription, one of the neurotrophic factors. These results indicate the diversity of targets affected by chronic exposure to corticosterone and highlight important regional differences in hippocampal neurobiology.

급성 족관절 염좌에 대한 복합 침치료의 효과: 사례군 연구

조남훈 ( Nam Hoon Cho ),김미령 ( Mi Riong Kim ),정훈 ( Hoon Jeong ),김동섭 ( Dong Sub Kim ),김은수 ( Eun Soo Kim ),박지용 ( Ji Yong Park ),박현민 ( Hyun Min Park ),이진호 ( Jin Ho Lee ),하인혁 ( In Hyuk Ha ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.1

The purpose of this study is to report the effect of combined acupuncture treatment on acute ankle sprain patients. The subjects included in this study were 15 acute ankle sprain patients who visited Jaseng Hospital of Korean Medicine from Oct. 15th, 2012 to Feb. 8th, 2013. We treated 15 patients with combined acupuncture treatment. The treatment con-sisted of Hwangrunhaedok-tang (Huanglianjiedutang) pharmacupuncture, electroacupunc-ture (applied to GB39, ST36 on the affected side), acupuncture (applied to TE17, SI6 on the unaffected side with Dong-Qi therapy). To assess the effect of treatment, the numeric rating scale (NRS) was applied before and after treatment. After first combined acupuncture treat-ment, the NRS scores significantly decreased from 8.33±0.94 to 2.26±0.44 (p<0.01). We suggested that Combined acupuncture treatment are effective and useful on acute an-kle sprain. And, further studies will be needed. (J Korean Med Rehab 2014;24(1):119-123)

소아의 메타콜린 기관지유발검사에서 PC₂₀-FEF_25%-75% 및 △FVC에 대한 분석

김현아 ( Hyeon A Kim ),권정은 ( Jung Eun Kwon ),안지영 ( Ji Young Ahn ),최재영 ( Jae Young Choe ),김동섭 ( Dong Sub Kim ),박숙현 ( Sook Hyun Park ),현명철 ( Myung Chul Hyun ),최봉석 ( Bong Seok Choi ) 대한천식알레르기학회(구 대한알레르기학회) 2021 Allergy Asthma & Respiratory Disease Vol.9 No.3

Purpose: Forced expiratory flow between 25% and 75% (FEF_25%-75%) is known to sensitively reflect bronchial obstruction. Methacholine challenge test (MCT) has shown varying reduction levels of forced vital capacity (FVC) with the reduction in forced expiratory volume in 1 second (FEV₁) in asthma. The aim of this study was to evaluate the clinical implication of provocative concentration causing a 20% fall in FEF_25%-75% (PC₂₀-FEF_25%-75%) and the percentage fall in FVC at the PC₂₀ dose of methacholine (△FVC). Methods: A total of 194 children who visited the hospital due to respiratory symptoms and underwent MCT were analyzed retrospectively. The patients were divided into 3 groups. Group I had both PC₂₀-FEV₁ and PC₂₀-FEF_25%-75% above 16 mg/mL; group II had a PC₂₀-FEF_25%-75% that fell below 16 mg/mL but PC₂₀-FEV₁ was 16 mg/mL or above; group III had a PC₂₀-FEV₁ and a PC₂₀-FEF_25%-75% that both fell below 16 mg/mL. Results: In group II, PC₂₀-FEV₁ was lower (P=0.026) and the rate of change in FEV₁ and FEF_25%-75% from baseline to 16 mg/mL of methacholine concentration was greater than in group I (both P<0.001). Levels of PC₂₀-FEF_25%-75% were higher in group II compared to group III (P<0.001). △FVC showed a correlation with PC₂₀-FEV₁ (P<0.001) only in the whole group. Conclusion: In asthmatic children, PC₂₀-FEF_25%-75% may be associated with bronchial hyperresponsiveness. △FVC was not associated with other parameters in either group. For subjects with a positive finding of PC₂₀-FEF_25%-75% and a negative finding of MCT, the progression to asthma can be suspected. (Allergy Asthma Respir Dis 2021;9:141-147)

대학생들의 개인별 특성이 외식 메뉴선택속성에 미치는 영향- 의정부 지역 중심으로 -

김동섭(Kim, Dong-Sub) 한국외식경영학회 2018 외식경영연구 Vol.21 No.6

본 연구는 의정부지역 대학생들의 개인별 특성이 외식 메뉴선택속성에 미치는 영향을 규명하기 위해서이다. 연구의 목적을 달성하기 위하여 의정부지역 4년제, 2년제 대학생 250명을 대상으로 총 232부의 설문지 회수하여 사용하였고, 결과를 도출하기 위해 신뢰성 분석, 요인분석, 상관분석, 회귀분석을 통해 유의성을 조사하고, 다음과 같이 요인을 분석하였다. 검증결과, 개인별 특성의 식생활 라이프는 5가지 요인(외식향유형 스타일, 일반 식당이용 스타일, 음료 및 후식이용 스타일, 단체급식이용 스타일, 내식이용 스타일)으로 도출하였고, 메뉴선택속성은 4가지 요인으로 건강추구형, 경제가치추구형, 유행추구형, 권유추구형으로 도출하였다. 연구 결과, 개인별 특성은 외식 메뉴선택속성에 영향을 미치는 것으로 나타났다. 대학생들의 개인별 특성에 따른 메뉴선택속성은 외식향유형 스타일은 건강 관심 메뉴, 경제적인 메뉴, 유행하는 메뉴, 추천하는 메뉴에 관심이 있고, 일반식당 이용 스타일은 건강 관심 메뉴, 유행하는 메뉴, 추천하는 메뉴에 관심이 있고, 음료 및 후식이용 스타일은 추천하는 메뉴에만 관심이 있고, 단체급식이용 스타일은 건강과의 관계가 있는 메뉴, 경제적인 메뉴에 관심이 있고, 내식이용 스타일은 건강과의 관계가 있는 메뉴에만 관심이 있는 것으로 조사되었다. 이러한 연구조사를 기반으로 지역의 외식기업과 정보를 공유하여 새로운 마케팅 방안으로 탐색할 수 있으리라 사료된다. The purpose of this study was to investigate the effect of Individual characteristics of college students in the Uijeongbu area on the Menu of selection attributes. In order to achieve the purpose of the study, a survey of 232 students was conducted to investigate the significance and the following factors were analyzed. The results of research are as follows: Individual characteristics was conducted from 5 factors: enjoyable eating, general restaurant, beverage and dessert, cafeteria meal, home meal style. For the menu selection attribute 4 factors including healthy seeking, economic value seeking, popularity seeking, recommendation seeking type was found. The results of this study was derived that the effect of individual characteristics on menu selection attributes. The enjoyable eating style is interested in the healthy menu, economical menu, trendy menu and recommended menu. General restaurant meal style is interested in the healthy menu, trendy menu, recommended menu. The beverage and dessert style is interested in the recommended menu, and the style of using the cafeteria meal is interested in the healthy menu, the economic menu. The style of the home meal is interested in the healthy menu. It is suggested that sharing restaurant decision related lifestyle of university students and explore them as a new marketing method based on these researches.

Green Growth - Promising Path of Sustainable Economic Growth

김동섭(Dong Sub Kim) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5

실시간 모니터링 및 고장 감지 가능한 협동 로봇 액추에이터 신뢰성 평가 시스템 개발

김동섭(Dong-Sub Kim),김태근(Tae-Keun Kim),서우석(Woo-Seok Seo),김병표(Byoung-Pyo Kim),박종범(Jong-Bum Park) 대한전자공학회 2019 대한전자공학회 학술대회 Vol.2019 No.11

로봇용 구동 모듈의 신뢰성 시험 시스템 개발

김동섭(Dong-Sub Kim),김태근(Tae-Keun Kim),서우석(Woo-Seok Seo),김병표(Byoung-Pyo Kim),박종범(Jong-Bum Park) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12

HBsAg 양성인 환자에서 Pre - S ( 2 )Ag 의 임상적 의의

김동섭(Dong Sub Kim),김경희(Kyung Hee Kim),최우창(U Chang Choi),이관식(Kwan Sik Lee),문영명(Young Myoung Moon),최흥제(Heung Jai Choi) 대한내과학회 1989 대한내과학회지 Vol.36 No.5

N/A The Pre-S(2) gene in the HBV DNA genome encodes a peptide in the HBV envelope which is called Pre-S(2) Ag, and this antigenie determinant stimulates the formation of anti-Pre-S(2). It has been reported that Pre -S(2)Ag appears in the early phase of acute viral hepatitis with HBV DNA, HBeAg, and there is a correlation between the titer of HBV DNA and that of Pre-S(2)Ag. We collected a total of 73 cases with a diagnosis of acute viral hepatitis, chronic hepatitis, liver cirrhosis, or asymptomatic carrier from April to September 1988 in order to check Pre-S(2)Ag, anti-Pre-S(2), HBsAg, anti-HBs, HBeAg, and anti-HBe, HBV DNA. The following results were obtained: 1) Pre-S(2)Ag was seen in 60 cases(82.1%) of 73 HBsAg positive patients. 2) Anti-Pre-S(2)Ag was not detected in all cases. 3) Pre-S(2)Ag was seen in 92.3% of HBeAg patients patients and in 60% of anti-HBe positive patoents. There was a statistical significance between the two groups (p < 0.05). 4) The mean value of Pre-5(2)Ag was 1.047±1.007 O. D. in HBeAg(+) patients and 0.369+0.463O.D. in anti -HBe(+) patients. There is a statistical significance between the two groups(p<0.01). 5) Pre-S(2)Ag was seen in 92% of HBV DNA(+) patients and in 75% of HBV DNA(-) patients and this was a statistical significance (p<0.05). 6) There was no relationship between the titer of HBV DNA and Pre-S(2)Ag. 7) The mean value of Pre-S(2) Ag w as 1.201±0.93 O.D. in HBV DNA(+) patients and 0582±0.785O.D. in HBV DNA(-) patients. There was no statistical significance. In concluion, Pre-S(2)Ag seemed to reflect the replication of the virus but not the degree of replication. Pre-S(2)Ag was suggested to have no more significance than HBV DNA or DNA polymerase, Pre-S(2)Ag disappeared later than HBV DNA, but earlier than HBsAg. We need a long-term follow-up study to deter- mine prognostic value with anti-Pre-S(2).

대학생들의 웰빙인식과 웰빙트렌드가 메뉴선택 속성에 미치는 영향 : 의정부지역 중심으로

김동섭(Kim, Dong-Sub) 한국외식경영학회 2019 외식경영연구 Vol.22 No.6

본 연구는 의정부지역 대학생들의 개인별 특성이 외식 메뉴선택속성에 미치는 영향을 규명하기 위해서이다. 연구의 목적을 달성하기 위하여 의정부지역 4년제, 2년제 대학생 250명을 대상으로 총 217부의 설문지 회수하여 사용하였고, 결과를 도출하기 위해 신뢰성 분석, 요인분석, 상관분석, 회귀분석을 통해 유의성을 조사하고, 다음과 같이 요인을 분석하였다. 검증결과, 웰빙인식과 웰빙트렌드는 6가지 요인( 사회지향성, 기대효과, 건강지향성, 매스컴반영, 개인성향, 안전추구)으로 도출하였고, 웰빙과 메뉴선택속성은 2가지 요인으로 건강관심형, 유행추구형으로 도출하였다. 연구 결과, 대학생들의 웰빙인식과 관련된 건강관심형·유행추구형의 메뉴선택속성은 사회지향성, 기대효과, 건강지향성에 관심을 갖는 것으로 조사되었다. 웰빙트렌드와 관련된 건강관심형·유행추구형의 메뉴선택속성은 매스컴반영, 개인성향, 안전추구에 관심이 있는 것으로 조사되었다. The purpose of this study was to investigate the effect of university students’ well-being Awareness and well-being trend on the Menu Selection attributes in the Uijeongbu area. In order to achieve the purpose of the study, a survey of 217 students was conducted to investigate the significance and the following factors were analyzed. The results of research are as follows: Well-being Awareness, Well-being trend, Menu Selection attributes was conducted from 8 factors : Social Directivity, Healthy Directivity, Expectation, Effectiveness, Reflection of Mass communication, Individual Characteristics, Pursuit of safety, Type of Healthy Concern. The results of this study was derived that type of Healthy, Inclined to fashion of the Menu Selection attributes if interested in the Social Directivity, Expectation effectiveness, Healthy directivity and thpe of healthy Concern, Inclined to fashion of Well-being trend was interested in Reflection of Mass communication, Individual Characteristics, Pursuit of Safety. It is suggested that sharing restaurant marketing, relted univerisity students and using a new promotion method based on these researches.

환자대면서비스에 대한 의사의 중요도 인식과 실천간 차이에 미치는 요인 분석

김동섭 ( Dong Sub Kim ),강혜영 ( Hye Young Kang ),이해종 ( Hae Jong Lee ) 한국보건행정학회 2008 보건행정학회지 Vol.18 No.3

To examine the gap between physician`s perceived importance and performance of care and to identify factors associated with the gap. Aself-administered questionnaire survey was conducted with 91 physicians working in a University hospital in Seoul. The respondents were asked about their perceived importance and actual performance of interpersonal care on a 5-point Likert-type scale, indicating a higher score as higher importance and performance. Interpersonal care was measured by questions modified from the Korean Standard Service Quality Index, which are grouped into 6 categories: basic services, extra services, reliability, courtesy, convenience, and tangibles. Multiple regression analysis was conducted to find out physician characteristics associated with the gap. All of the 6 interpersonal care categories showed lower performance than perceived importance. The respondents tended to have a worse performance than perceived importance as the number of patients per outpatient care session (β=-0.0204, p<0.05) and the need for customer satisfaction education increase (β=-0.2226, p<0.05). Female physicians (β=0.2336, p<0.05) and those with higher job satisfaction(β=0.0096, p<0.05) showed a better performance than perception. Overall, it appears that lower quality of interpersonal care was delivered to patients than the desired level considered by the responding physicians. Based on the regression analysis results, it is suggested that reducing patient volume per session, fulfilling education need for customer satisfaction, and improving job satisfaction may contribute to reduce the gap between physician`s perceived importance and performance of interpersonal care.