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보골지 추출물이 파골세포 분화 및 골흡수 관련 유전자 발현에 미치는 영향
류광현 ( Gwang-hyun Ryu ),김엄지 ( Eom Ji Kim ),김민선 ( Minsun Kim ),김재현 ( Jae-hyun Kim ),이유진 ( Yujin Lee ),진대환 ( Dae-hwan Jin ),손영주 ( Youngjoo Sohn ),정혁상 ( Hyuk-sang Jung ) 경락경혈학회 2021 Korean Journal of Acupuncture Vol.38 No.3
Objectives : The increase of osteoclasts could cause osteoporosis and bone-related diseases. Also, the inhibition of osteoclast differentiation is important in treating bone-related diseases. Traditionally, Psoraleae Semen has been used for geriatric diseases, aging and musculoskeletal diseases. The purpose of this study is to investigate the effect of Psoraleae Semen ethanol extract (PS) on osteoclast differentiation and its function. Methods : To confirm the effect of PS on osteoclastogenesis and bone resorption activity, various levels of concentrations of PS (5, 10, 20 and 40 μg/ml) were tested on RAW 264.7 cells cultured with RANKL. We measured tartarate-resistant acid phosphatase (TRAP)-positive cells, TRAP activity, pit formation and F-actin ring formation. The expressions of nuclear factor of activated T-cells (NFATc1) and c-Fos were confirmed through western blot and reverse transcription- polymerase chain reaction (RT-PCR). Also, the expression of bone resorption and fusion-related genes in osteoclast was confirmed by RT-PCR. Results : PS decreased the number of TRAP-positive cells and the TRAP activity. In addition, PS significantly inhibited the formation of pit and F-actin ring. Furthermore, PS decreased the expression of osteoclast related genes. Conclusions : PS inhibits osteoclast differentiation and bone resorption ability through inhibition of the expression of osteoclastrelated genes. This indicates that PS may be a potential therapeutic agent to osteoporosis by suppressing osteoclastogenesis.