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인동(忍冬)의 기철(忌鐵) 및 반철(伴鐵) 추출시(抽出時) 추출물(抽出物)의 변화(變化)
정덕자,정대화,장미희,박정아,김상찬,Jeong, Deok Ja,Jung, Dae Hwa,Jang, Mi Hee,Park, Chung A,Kim, Sang Chan 대한한의학방제학회 2020 大韓韓醫學方劑學會誌 Vol.28 No.3
Objectives : In case of herbs decoction, the ceramic or earthware pots was recommended, but not the metals, particularly iron or aluminum, which could cause unknown chemical reactions. In Korean Medical classics, it has been known that some herbs including Caulis Lonicerae Japonicae (CL) were not recommended to boil with iron pot. This study investigates the physical changes of extracts of CL with or without iron. Methods : CL was decocted with reflux cooling extraction system to prevent evaporation and volatilization. Content of polyphenol was detected by Folin-Denis method and the levels of loganin and chlorogenic acid were evaluated by UPLC. Results : The color of extract with glass beads (GB) is yellowish brown, and the iron beads (IB) is blackish brown. Polyphenol and chlorogenic acid levels were reduced in IB extracts. Conclusions : The color of extract was change to blackish brown, and polyphenol and chlorogenic acid levels were reduced in CL extract with iron. Therefore, iron pots is not suitable for CL extraction.
유영은,박은영,정대화,변성희,김상찬,박성민,Yu, Young-Eun,Park, Eun-Young,Jung, Dae-Hwa,Byun, Sung-Hui,Kim, Sang-Chan,Park, Sung-Min 한국미생물학회 2010 미생물학회지 Vol.46 No.2
본 연구에서는 69가지의 한약재를 이용하여 구강세균인 Actinomyces viscocus, Streptococcus sobrinus, 그리고 Streptococcus mutans 2종에 대한 항균효과를 조사하였다. 우선 선발된 한약재를 메탄올로 추출하여 조사하였으며 그 결과를 바탕으로 소목, 오매, 오미자, 그리고 황련을 선별하여 열수, 에탄올, 메탄올, 그리고 에틸아세테이트를 이용하여 항균효과를 조사하였다. 조사결과 황련 열수 추출물이 가장 우수한 효과를 나타내는 것으로 조사되었다. 황련 열수 추출물을 이용하여 공시균주에 대한 저해양상을 조사한 결과 180 mg/ml 이상의 농도에서 공시균주의 생육이 대부분 저해되는 것을 확인할 수 있었으며 이 때 황련 열수 추출물의 회수율은 22%였다. 황련 열수 추출물을 UPLC를 이용하여 berberine 함량을 조사한 결과 25.54%로 조사되었다. 또한 $121^{\circ}C$에서 15분간 열 처리 후 황련 열수 추출물의 열 안정성을 조사한 결과 열에 대하여 안정 하다는 것을 확인할 수 있었다. In this study, we investigated the antibacterial effect of 69 oriental medicinal herbs (OMHs) on Actinomyces viscocus, Streptococcus sobrinus, and two strains of Streptococcus mutans as oral bacteria. Methanol extracts of OMHs were used in the first antibacterial test, and then boiled water, ethanol, methanol, and ethyl acetate extracts of C. japonica, C. sappan, P. mume, and S. chinensis were used in the second test. Boiled water extract of C. japonica was shown to have the most superior effect on oral bacteria. The extract yield of boiled water extract of C. japonica was 22%, and the growth of oral bacteria was almost inhibited at over a 180 mg/ml concentration. Antibacterial effect compound analyzed by UPLC method was identified as berberine, and the content was 25.54%. In addition, the extract appeared to be stable at $121^{\circ}C$ for 15 min.
지칭개에서 분리한 Hemistepsin A와 B의 비듬균에 대한 항균효과
이종록(Jong Rok Lee),정대화(Dae Hwa Jung),박문기(Moon Ki Park) 한국생물공학회 2013 KSBB Journal Vol.28 No.2
There are a large number of bioactive sesquiterpene lactones from compositae plants including Hemistepita lyrata Bunge. In the present study, we purified two sesquiterpene lactones, Hemistepsin A and B, from H. lyrata and evaluated their antimicrobial activities against Malassezia obutusa. Chromatographic separation was used for the preparation of Hemistepsin A and B, and the identity of these compounds was confirmed by NMR. Strong inhibition of growth of M. obutusa was obtained with all doses of Hemistepsin A tested. Moreover, antifungal activity of Hemistepsin A occurred in a dose-dependent manner. Hemistepsin B also showed potent antifungal activity at the dose of 800 μm/disc. From these results, it was suggested that Hemistepsin A and B be beneficial for the preparation of the useful agent for treating scalp diseases occurred by dandruff-causing Malassezia species.
간 실질세포주에서 니호채(泥胡菜) 분획물이 liver X receptor α 의존적 지방 생성 유전자의 발현에 미치는 효과
김재광,조일제,김은옥,정대화,구세광,김상찬,Kim, Jae Kwang,Cho, Il Je,Kim, Eun Ok,Jung, Dae Hwa,Ku, Sae Kwang,Kim, Sang Chan 대한한의학방제학회 2020 大韓韓醫學方劑學會誌 Vol.28 No.3
Objectives : Hemistepta lyrata Bunge (Bunge) is a wild herb that has been used for managing fever and wound in Korean Traditional Medicine. The present study explored the effects of H. lyrata extract on liver X receptor (LXR) α-dependent lipogenic genes in hepatocyte-derived cells. Methods : After HepG2 cells or Huh7 cells were pre-treated with 1-10 ㎍/mL of H. lyrata extract or its fractionated extract for 0.5 h, the cells were subsequently exposed to LXR ligand for 6-24 h. Cell viability, LXR response element (LXRE)-driven luciferase activity, sterol regulatory element binding protein-response element (SREBP-RE)-driven luciferase activity, SREBP-1c expression, and mRNA levels of LXRα and its-dependent target genes were determined. In addition, LC-MS/MS analysis was conducted to explore major compounds in H. lyrata-chloroform fractionated extract #4 (HL-CF4). Results : Of various H. lyrata extracts tested, chloroform extract and its fractionated extract #4, HL-CF4, significantly decreased T0901317-mediated SREBP-1c expression. In addition, HL-CF4 significantly reduced LXRE atransactivation and LXRα mRNA expression without any cytotoxicity. Moreover, HL-CF4 prevented the SREBP-RE-driven luciferase activity and mRNA levels of fatty acid synthase and stearoyl-CoA desaturase-1 induced by T0901317. Results from LC-MS/MS analysis at positive/negative mode indicated that HL-CF4 contained several compounds showing m/z 197.1176 (C<sub>11</sub>H<sub>17</sub>O<sub>3</sub>), 693.2913/227.1069 (C<sub>38</sub>H<sub>45</sub>O<sub>12</sub>/C<sub>15</sub>H<sub>15</sub>O<sub>2</sub>), 203.1797 (C<sub>15</sub>H<sub>23</sub>), 181.1225 (C<sub>11</sub>H<sub>17</sub>O<sub>2</sub>), 591.2957 (C<sub>35</sub>H<sub>43</sub>O<sub>8</sub>), 379.1040 (C<sub>18</sub>H<sub>19</sub>O<sub>9</sub>), 409.1509 (C<sub>20</sub>H<sub>25</sub>O<sub>9</sub>), 309.1348 (C<sub>16</sub>H<sub>21</sub>O<sub>6</sub>), 391.1404 (C<sub>20</sub>H<sub>23</sub>O<sub>8</sub>), and 669.2924/389.1248 (C<sub>36</sub>H<sub>45</sub>O<sub>12</sub>/C<sub>20</sub>H<sub>21</sub>O<sub>8</sub>). Conclusion : Based on its inhibition of the LXRα-dependent signaling pathway, H. lyrata chloroform extract and HL-CF4 have prophylactic potentials for managing non-alcoholic fatty liver.
박성민,이원욱,조미정,이종록,정대화,박은영,변성희,김상찬,Park, Sung-Min,Lee, Won-Wook,Jo, Mi-Jeong,Lee, Jong-Rok,Jung, Dae-Hwa,Park, Eun-Young,Byun, Sung-Hui,Kim, Sang-Chan 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.1
Gyoungok-go (GOG; Qiongyu-gao in Chinese) has been used in traditional oriental medicine for the improvement of various physiological vitality functions. The aim of present work is to investigate a processing possibility of vinegar using GOG. GOG vinegar was prepared by two stage fermentations of alcohol and vinegar. The method of GOG fermentation with polished rice as a fermentation substrate is not yet established. The result of alcohol fermentation rate of GOG with some strains, strain No. 1 produced the highest alcohol contents among 7 strains. The strain No. 1 identified Saccharomyces paradoxus by 18S rDNA classification. S. paradoxus was produced over 20% alcohol in the 1$\sim$11% GOG with polished rice. A. aceti was produced 8.52% acetic acid from 6.5% alcohol containing 11% GOG, after incubation for 11 days.
김선영 ( Seon Young Kim ),정대화 ( Dae Hwa Jung ),백영두 ( Young Doo Back ),박상미 ( Sang Mi Park ) 대구한의대학교 제한동의학술원 2015 東西醫學 Vol.40 No.3
In the present study, we have demonstrated the anti-inflammatory effects of Bambusae Caulis In Liquamen (BCIL) in macrophage cell line. To investigate the anti-inflammatory activity, we examined the effects of the lipopolysaccaride (LPS)-induced production of nitric oxide (NO), pro-inflammatory cytokines and prostaglandin E2 (PGE2) in a murine macrophage cell line Raw 264.7. The Raw 264.7 cells were cultured in DMEM + serum medium for 24 hr. After serum starvation for 24 hr, the cells were treated with BCIL 1, 3, 9 (‰) for 1 h, followed by stimulation with LPS (1 ㎍/㎖) for activation of immune response. After treatment, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. The levels of cytokine were analyzed by sandwich immunoassays. As results, BCIL has an inhibitory effect on the production of NO, PGE2, Tumor necrosis factor-α and Interleukin-6. These results suggest that BCIL can inhibit production of pro-inflammatory mediators and might be a useful source for treatment of acute inflammatory disease.
장미희 ( Mi Hee Jang ),정대화 ( Dae Hwa Jung ),박정아 ( Chung A Park ),박숙자 ( Sook Jahr Park ) 대구한의대학교 제한동의학술원 2018 제한동의학술원논문집 Vol.16 No.1
Objectives:Hottuynia cordata is a traditional herb that has various physiological activity such as anti-cancer and anti-inflammation. We examined the anti-inflammatory effect of Hottuynia cordata root ethanol extract (HRE) in LPS-stimulated Raw264.7 cells. Methods:Cell viability was measured by MTT assay. To detect nitric oxide (NO) production, culture media were mixed with Griess reagent and measured the absorbance at 540 nm. The levels of cytokines were determined with ELISA kit according to manufacturer's instructions. Western blot was applied to monitor protein expression levels. Results:LPS-induced NO production was dose-dependently decreased by the treatment of HRE. The inhibitions of NO was due to the suppression of inducible nitric oxide synthase (iNOS) via nuclear factor-kappaB (NFκB), when determined by Western blot analysis. In addition, HRE efficiently inhibited pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β. Conclusion:These results show that the root portion of Hottuynia cordata can be used as a herbal medicine with anti-inflammatory action.
자오정린,왕옌,린훵,후우후이,저우후우??,장수찬,한누리,정대화,양재하,김상찬,자오르옹지에,Zhao, ZhengLin,Wang, Yan,Lin, Feng,Fu, Hui,Zhou, FuBo,Chang, Suchan,Han, Nu Ri,Jung, Dae Hwa,Yang, Chae Ha,Kim, Sang Chan,Zhao, RongJie The Korean Medicine Society for the Herbal Formula 2014 大韓韓醫學方劑學會誌 Vol.22 No.1
Background and objective: Methamphetamine (Meth) is a widely abused psychostimulant that produces hyperlocomotion in rodents. Radix of Glycyrrhizae uralensis comprises a variety of bioactive components that have neuroprotective effects. In a previous study, we have demonstrated methanol extracts from radix of Glycyrrhizae uralensis (MEGR) suppress acute cocaine-induced extracellular dopamine release in the nucleus accumbens. In the present study, we investigated the effect of MEGR on acute Meth-induced hyperlocomotion. Methods: Male Sprague-Dawley rats were orally administered with MEGR (60 mg/kg and 180 mg/kg) 60 min prior to an intraperitoneal injection of Meth (1.0 mg/kg). Results: Behavioral analysis showed acute Meth greatly increased locomotor activities, while pretreatment with MEGR dose dependently inhibited the hyperlocomotion. In parallel, there were markedly increased levels of dopamine and its metabolite 3, 4-dihydroxyphenylacetic acid in the nucleus accumbens tissues in Meth-treated rats, which were also almost completely reversed by 180 mg/kg MEGR. Conclusions: These results showed that radix of Glycyrrhizae uralensis attenuates Meth-induced hyperlocomotion by inhibiting dopamine synthesis and utilization, suggesting that radix of Glycyrrhizae uralensis might be effective in blocking the rewarding effect of Meth.