주암호 지천의 수질과 어류군집

성치남,백근식,최지혁,조현욱,김종홍 ( Chi Nam Seong,Keum Sik Baik,Ji Hyeok Choi,Hyun Wook Cho,Jong Hong Kim ) 한국하천호수학회 1997 생태와 환경 Vol.30 No.2

Water quality and fish community in the streamlets of Juam reservoir were investigated in March, July, October and December 1997. Average value of dissolved oxygen, chemical oxygen demand and suspended solids was 10.1, 3.9 and 5.7 mg/l, respectively. Concentration of ammonium nitrngen varied with sites. Content of ammonium nitrogen, total nitrogen and phosphate-phosphorous of Site 4 where the waste water from Posung-up flows, was higher than that of any other sites. The number of collected fish species was 46 in 33 genera which belong to 12 families. Sixteen species in 13 genera were identified as Korean endemic species. Dominant species, Zacco platypus was collected in all sites. The largest number of species were collected in Site 10. Carassius auratus, Pseudorasbora parva and Z. platypus which have strong adaptablity to the environmental changes were collected in Site 4 where the species composition was simpler and the water was more polluted. Diversity and evenness indices ranged from 0.60 to 1.18 and from 0.28 to 0.75, respectively. These indices were low in the polluted sites. These results showed that water quality is related to some extent with species composition of fish.

계통학적 방벙을 이용한 우포습지의 원핵미생물 다양성

성치남(Chi Nam Seong),백근식(Keun Sik Baik),김미선(Mi Sun Kim),박성찬(Seong Chan Park) 한국토양비료학회 2006 한국토양비료학회 학술발표회 초록집 Vol.2006 No.5

계통학적 방법을 이용한 우포습지의 원핵미생물 다양성

성치남 ( Chi Nam Seong ),백근식 ( Keun Sik Baik ),김미선 ( Mi Sun Kim ),박성찬 ( Seong Chun Park ) 한국토양비료학회 2006 한국토양비료학회 학술발표회 Vol.2006 No.-

Seasonal Variations in the Bacterial Community of Gwangyang Bay Seawater

Seong Chan Park(박성찬),Ji Hee Lee(이지희),Joo Won Kang(강주원),Keun Sik Baik(백근식),Chi Nam Seong(성치남) 한국생명과학회 2014 생명과학회지 Vol.24 No.5

광양만 해수의 세균 군집의 계절적 변화를 배양법과 비배양법을 사용하여 분석하였다. 200개의 분리 균주에 대해 Amplified rDNA restriction 방법을 적용한 경우 분리 균은 Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes의 4개의 문에 속함을 확인하였다. 비 배양방법으로는 해수로부터 직접 추출한 DNA를 사용하여 pyrosequencing과 변성 농도구배 전기영동(DGGE)을 실시하였다. Pyrosequencing에 의한 16S rRNA 유전자의 염기서열 분석 결과 세균 군집은 춘계와 하계에 각각 24개, 추계에 39개 그리고 동계에 32개의 문으로 구성되었다. 다양도 지수는 추계에 높았으며 춘계에는 우점도 지수가 높았다. Firmicutes 문의 세균이 춘계에 예외적으로 많은 비율을 차지하였으며 나머지 계절에는 Proteobacteria 문의 세균이 우점하였다. 차 우점 분류군은 춘계에는 Proteobacteria 문의 세균인 반면 하계에는 Firmicutes 문, 추계와 동계에는 Bacteroidetes 문의 세균이 차지하였다. 과 수준에서의 우점 분류군은 Bacilliaceae가 춘계에, Rhodobacteraceae와 Bacilliaceae가 하계에, Rhodobacteraceae가 동계에 나타났으나 추계에는 우점 분류군이 없었다. DGGE 에서 확인된 27개의 DNA 절편을 추출하여 계통분석을 실시한 결과 춘계에는 Firmicutes 문에 이어 Proteobacteria 문이 우점하였으며 다른 계절에는 Proteobacteria 문이 우점하였다. 두 가지의 비배양법에 의한 군집 분석 결과 문 수준에서의 세균 군집의 계절적 변화는 유사한 경향이 나타났다. Seasonal variations in the bacterial community of Gwangyang Bay seawater were analyzed using both isolation and cultivation-independent methods. Amplified rDNA restriction analysis was applied to 200 bacterial isolates. Bacterial isolates were composed of four phyla: Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Pyrosequencing was conducted, in addition to denaturing gradient gel electrophoresis (DGGE) of genomic DNA extracted directly from the water samples. The bacterial sequences obtained by pyrosequencing of 16S rRNA genes consisted of 24 phyla in the spring and summer, 39 in the fall, and 32 in the winter. The diversity index was high in the fall, whereas the dominancy index was high in the spring. In the spring, phylum Firmicutes was dominant, whereas phylum Proteobacteria dominated in the other three seasons. The second most dominant phyla were Proteobacteria in the spring, Firmicutes in the summer, and Bacteroidetes both in the fall and winter. Bacilliaceae was the most predominant family in the spring. Rhodobacteraceae and Bacilliaceae dominated in the summer, and Rhodobacteraceae dominated in the winter. Neither was dominant in the fall Twenty-seven bands purified from DGGE profiles were cloned and analyzed phylogenetically. In the spring, phylum Firmicutes dominated, followed by Proteobacteria. Proteobacteria dominated in all other seasons. Thus, two cultivation-independent methods for determination of seasonal variation patterns at the phylum level were in accordance with each other.

Complete genome sequence of Neisseria sp. KEM232 isolated from a human smooth surface caries

김은미,성치남,Kim, Eun Mi,Seong, Chi Nam The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.1

Neisseria 속 균주 KEM232는 사람 평활면 치아우식 부위로부터 분리하였다. 균주 KEM232의 유전체는 G + C 비율이 58.5%, 2,369개의 유전자와 2,210개의 단백질 코딩 유전자, 108개의 위유전자, 51개의 RNA 유전자 그리고 한 개의 CRISPR array를 포함한 단일 원형 염색체로 구성되었으며 그 크기는 2,371,912 bp였다. 균주 KEM232의 최 근연종은 Neisseria baciliformis 로서 두 균주 사이의 16S rRNA 유전자 염기서열의 유사도는 96.8% 그리고 유전체의 평균 염기 동일성은 84%였다. We sequenced the genome of the Neisseria sp. KEM232 isolated from the smooth surface caries of human cavity of a 7-year old male in Republic of Korea by using the standard dilution plating technique. The genome comprises a single circular 2,371,912 bp chromosome with a G + C content of 58.5%, 2,210 protein-coding genes, 108 pseudo genes, 51 RNA genes, and one CRISPR array. Based on the 16S rRNA gene sequence similarity and average nucleotide identity, the strain KEM232 is most closely related to Neisseria baciliformis.

에탄올 발효를 위한 Zymomonas mobilis 균주의 개량에 관한 연구 - 원형질체 세포형성과 정상세포로의 재생조건 검토 -

이계준(Kye Joon Lee),성치남(Chi Nam Seong) 한국미생물생명공학회 1984 한국미생물·생명공학회지 Vol.12 No.2

Complete genome sequence of Flavivirga eckloniae ECD14^T isolated from a seaweed Ecklonia cava

이지희,강주원,김은미,성치남,Lee, Ji Hee,Kang, Joo Won,Kim, Eun Mi,Seong, Chi Nam The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.2

대한민국 남해에서 채집한 해조류 감태(Ecklonia cava)로부터 분리한 Flavivirga eckloniae $ECD14^T$ 균주의 유전체서열을 분석하였다. 균주 $ECD14^T$의 유전체는 G + C 비율이 33.9%이며, 4,647개의 유전자와 4,595개의 단백질 코딩 유전자, 44개의 위유전자, 52개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으며 그 크기는 2,371,912 bp였다. 파아지와 트랜스포존 유전자가 존재하며, CISPR array 관련 유전자 및 서열은 발견되지 않았다. 균주 $ECD14^T$는 해조 다당의 분해에 관여하는 alginate lyase와 ${\beta}$-glucosidase 유전자를 가지고 있었다. The genome of Flavivirga eckloniae $ECD14^T$ isolated from a seaweed Ecklonia cava was sequenced. The genome comprises a single circular 5,665,358 bp chromosome with a G + C content of 33.9%, 4,647 total genes, 4,595 protein-coding genes, 44 pseudo genes, and 52 RNA genes. CRISPER genes and sequences were not found and there were some phage remnants and transposons. This strain contains alginate lyase and ${\beta}$-glucosidase genes responsible for the degradation of seaweed polysaccharides.

DNA 염기서열 분석에 의한 떡차의 미생물 다양성 분석

백근식(Keun-Sik Baik),성치남(Chi-Nam Seong),황영민(Yeong-Min Hwang),김기안(Gee-An Kim),이나라(Na-Ra Lee),김두운(Duwoon Kim),조정용(Jeong-Yong Cho),김선재(Seon-Jae Kim),박근형(Keun-Hyung Park),문제학(Jae-Hak Moon) 한국차학회 2012 한국차학회지 Vol.18 No.3

To determine the microbiological characteristics of ddeok cha, which is known as a Korean traditional microbial-fermented tea, bacterial and fungal communities of ddeok cha were analyzed by using a pyrosequencing method. Total genomic DNAs were extracted from ddeok cha directly. Ribosomal RNA genes were amplified and sequenced using specific primers for bacteria and fungi. A total of 12,851 reads (bacteria, 8,995; fungi, 3,586) were compared with known sequences in the database. Bacteria were assigned to five phyla, 25 families, 44 genera, and 104 species, whereas fungi were to five phyla, 41 families, 51 genera, and 69 species. Almost all of the bacteria (98.63%) were Gram-negative and members of the phylum Proteobacteria, of which the predominant genus was Pantoea (89.02%) followed by Erwinia (6.13%). Almost all of the fungi (98.86%) were acomycetes such as Dothideomycetes (60.97%) and Eurotiomycetes (31.55%). In this study, we found that microflora of ddeok cha could be distinguished from the predominant microflora (Aspergillus, Penicillium and yeast, Candida) of Pu-erh tea.

중환자실의 임상검체로부터 분리된 Methicillin 내성 Staphylococcus aureus의 독소유전자형과 항생제내성의 상관관계

박철 ( Chul Park ),성치남 ( Chi Nam Seong ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.3

본 연구는 methicillin-resistant Staphylococcus aureus(MRSA)로부터, 독소 유전자형과 항생제 내성의 상관 관계를 결정하는 것을 목표로 하였다. 2014년 1월∼12월까지 전남 순천의 한 병원 중환자실의 임상검체 2,664건에서 얻어진 MRSA 52균주를 분리하였다. 유전자들이 암호화하고 있는 mecA, 장독소(staphylococcalenterotoxins; sea, seb, sec, seg, seh, sei, sej), 독성 쇼크 증상독소-1 (toxic shock syndrome toxin-1; tst-1), 표피박탈성독소(exfoliative toxin; eta, etb), 백혈구 용해 독소(Panton-Valentine leukocidin; pvl)를 특이적 프라이머를 이용한 multiplex PCR로 증폭 검출 하였다. 독소 유전자 seg와 sei 유전자가 각각 40균주(76.9%)로 가장 많은 보유율을 나타냈으며 다음으로 tst 34균주(65.4%) 순으로 검출 되었으며 eta, etb, sea, sed, see, seh, sej와 pvl 유전자들은 검출 되지 않았다. 2개 이상의 독소유전자를 동시에 보유한 조합의 MRSA는 40균주(76.9%) 였는데 5개 유전자(seb, sec, seg, sei, tst)를 동시 보유한 조합이 28균주(53.8%)로 가장 많은 분포를 보였으며 다음으로 seg, sei 유전자 동시 보유 조합으로 6균주(11.5%)에서 나타났다. 유전자들 간의 동시 보유율은 72.5∼100%로서 특정한 독소 유전자 seb, sec, seg, sei와 tst 유전자간의 상관성이 높게 나타났다. 특정 다수의 독소유전자(seb, sec, seg, sei, tst)를 동시에 보유한 균주들이 개별적 독소유전자를 보유한 균주(seb, sec, tst)와의 항생제 내성의 상관성은 ciprofloxacin, clindamycin, erythromycin 항생제에 100% 내성을 보임으로서 공통적으로 포함된 seb, sec, tst 유전자와 이 항생제의 내성과는 밀접한 연관이 있음을 알았다. This study is aimed to determine the correlation between the toxin gene types and antibiotic resistance from MRSA (methicillin-resistant Staphylococcus aureus). Fifty-two strains of MRSA, between January 2014, and December 2014, were isolated from clinical specimens obtained from 2,664 cases in the intensive care unit of a hospital in Suncheon, Jeonnam, Korea. Genes encoding mecA, enterotoxin (SE), toxic shock syndrome toxin-1 (TSST-1), exfoliative toxin (ET), and Panton-Valentine leukocidin (PVL) were detected by multiplex PCR-mediated amplification using specific primers. Toxin genes (seg and sei) were present in 40 strains (76.9%), followed by tst in 34 strains (65.4%). Other genes (eta, etb, sea, sed, see, seh, sej, and pvl) were not detected. Forty strains (76.9%) of MRSA had 2 or more toxin genes simultaneously; 5 coexistent toxin-genes (seb, sec, seg, sei, tst) were the most common in 28 strains (53.8%), and 6 strains (11.5%) had seg and sei genes. The coexistence of genes were 72.5∼100%, showing a high correlation among genes (seb, sec, seg, sei and tst). As strains (seb, sec, tst) that had particular toxin genes (seb, sec, seg, sei, tst) in multiple showed 100% resistance to ciprofloxacin, clindamycin, erythromycin, we were able to find that seb, sec, and tst genes have a close relationship to the aforementioned antibiotics. It showed a higher resistance to ciprofloxacin, clindamycin, erythromycin, and tetracycline compared with strains that had toxin genes independent from multiple toxin genes.

퀴롤논 내성 Campylobacter jejuni의 분리와 향균제 감수성

김은철 ( Eun Chul Kim ),심은숙 ( Eun Sook Shim ),성치남 ( Chi Nam Seong ),김신무 ( Shin Moo Kim ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2

Campνlobacter spp. are frequent cause of enteric infecton. Increased resistance of human isolates of Campνlobacter was considered in part due to widespread animal use of antimicrobial agents. In Korea, our knowledge on both human and animal carriage of Campνlobacter is veη limited. In this study, 260 stool specimens were taken from hea1thy subjects in Soonchun and Iksan city, and intestinal contents of 231 chickens during the period between August 1998 to July 1999. The Isolaton rate of Campνlobacter from hea1thy subjects was 1.9%, and the rate was higher in :::;; 10-year-old children. The isolation rate from healthy chicken was 66.3%, and the rate was highest in August (80%) and lowest in December (56.5%). All of the C. j밍Uni from chicken were susceptible to amikacin, c1indamycin and gentamicin. The resistance rates were 97% to tetracyc1ine, 82% to ciprofloxacin, nalidixic acid and co-trimoxazloe, 65% to ampicillin. All of the C. jejuni isolates from human were resistant to ampicillin and co-trimoxazole, while the rates were 67% for both ciprofloxacin and tetracycline. For C. jejuni, antimicrobial agents with lower MICs were imipenem :::;;0.06 μg/ml , gentamicin 0.25 μg/ml , amikacin 0.5-4 μg/ ml and kanamycin 2-8 μg/ml. MIC ranges of ciprofloxacin and nalidixic acid were 0.06-64 μg/ ml and 32-64 μg/ml , respectively. Susceptibilities to nalidixic acid and to ciprofloxacin were identical Ampici1lin and tetracyc1ine resistance rates were higher among nalidixic acid-resistant isolates than susceptible ones. In conclusion, human carriage rate of Campνlobacter is veη low, while the rate in chickens is veη hi맹. Fluoroquinolone resistance rate of C. jejuni is very high, but amikacin and gentamicin can be used empirically without susceptibility testing as resistance to these antibiotics seem to be rare.