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유창연(Chang Yeon Yu),이현용(Hyeon Yong Lee),이미경(Mi Kyoung Lee),이현수(Hyun Soo Lee),최근표(Geun Pyo Choi),오덕환(Deog Hwan Oh),김종대(Jong Dai Kim) 한국약용작물학회 2003 한국약용작물학회지 Vol.11 No.1
The biological activities of extracts from Rubus coreanus Miq. ere compared, About 70% of the growth of human hepatocarcinoma and 79% of human gastric cancer call was inhibited in adding 1.0㎎/ml of the extracts of Rubus coreanus Miq. respectively. The growth of human breast cancer cells was also inhibited in adding 1.0 ㎎/ml of the extracts as well as 78% of the human cancer cells. It was proved that the growth of human normal lung cell, scored as 15% for the extracts. Overall selectivity of the extracts on several human cancer cell line was over 5, which is higher than those from the Rubus coreanus Miq. The growth of both human immune B and T cells was enhanced up to 1.4 to 1.8 times by adding the extracts, compared to the controls. The secretion of tumor necrosis factor-alpha(TNF-α) from T cell was also increased up to 78.8pg/ml in adding the ethanol extract (0.5 ㎎/ml). Ethanol extracts also increased up to about 70 pg/ml of interleukin-6(IL-6) from B cell. For screening regulate function of blood pressure, angiotensin converting enzyme(ACE) activity was inhibited up to 25% by adding ethanol extract (1.0 ㎎/ml). In testing the hypoglycemic activity, 20% of α-glucosidase activity was inhibited for the extracts (0.5 ㎎/ml). GST activity was increased in the range of 1.2 to 1.6 times by adding extracts.
Acifluorfen 抵抗性 토마토(lycopersicon peruvianum mill) 細胞株 選拔 및 植物體 分化
유창연(Chang Yeon Yu),조혜경(Hye Kyoung Cho) 한국육종학회 1994 한국육종학회지 Vol.26 No.2
Fifteen acifluorfen-tolerant cell lines of Lycopersicon peruvianum were isolated by stepwise selection in suspension cultured cells. Growth of unselected line was completely inhibited at 0.5μM, while selectd callus lines grew at 8μM acifluorfen. After subculturing on acifluorfen-free medium for 5 passage(over 4 months), eleven cell lines tested maintained their tolerance to 0.5μM acifluorfen. The regeneration capacity of acifluorfen-tolerant cell lines differed depending on the cell line. Seven of 14 cell lines regenerated the shoot and six cell lines did not. Selected cell lines were more effective than the unselected control callus in regenerating on medium containing acifluorfen. The unselected callus of L. peruvianum did not regenerate shoots on medium containing over 1μM acifluorfen, but selected lines regenerated shoots on medium with 5μM acifluorfen. The addition of 10㎎/L spermidine to the medium improved the regeneration rate and the growth of acifluorfen-tolerant lines.
알로에(aloe vera L.) 生長點培養에 있어 生長調節物質 및 培地가 植物體分化와 生長에 미치는 影響
유창연(Chang Yeon Yu),조혜경(Hye Kyoung Cho),안상득(Sang Deuk Ahn) 한국육종학회 1994 한국육종학회지 Vol.26 No.3
This study was conducted to investigate the optimum medium and levels of growth regulators for multiple shoot induction as a rapid micropropagation system from meristem culture of Aloe vera L. in vitro. MS medium supplemented with 3㎎/ℓ BA was effective for shoot regeneration and addition of 0.1μM TDZ increased the number of shoots but retarded the growth of shoot. More shoots were regenerated when 2㎎/ℓ BA combined with 0.1㎎/ℓ IAA than when only cytokinin were added onto the medium. MS medium containing IAA and NAA was effective for rooting compared with medium containing no growth regulators. When regenerated plantlets were transplanted on pot, the survival rate of plants was 75% on perlite and 95% on vermiculite, respectively.
가축분퇴비가 연용된 밭 토양에서 잠재적 질소 무기화량 추정
윤홍배(Hong-Bae Yun),이연(Youn Lee),유창연(Chang-Yeon Yu),양재의(Jae-E Yang),이상민(Sang-Min Lee),신재훈(Jae-Hun Shin),김석철(Suk-Chul Kim),이용복(Yong-Bok Lee) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.3
The characteristics of nitrogen mineralization in upland soil was studied with 27-week incubation at 25℃. The used soils in this experiment were received six kinds of livestock manure compost each year for four years. Six different composts, which were chicken (CHM), pig (PIM), and cow (COM) manure composted without bulking agent, and chicken (CHMS), pig (PIMS), and cow (COMS) manure composted with sawdust as a bulking agent, were selected for this study. The first-order model was fit to the observed mineral nitrogen (N) vs incubation days using a non-linear regression procedure. The soil potential for N mineralization (No) of manure compost (CHM, PIM, and COM) treated soils were higher than those of the manure-sawdust compost (CHMS, PIMS, and COMS) treated soils. The No value of PIM applied soil was 15.0 mg 100 g<SUP>-1</SUP>, which was the highest value among the treatments. The amount of N mineralized in compost applied soils ranged from 8.1% to 11.9% of the total N content in soils and increased with increasing total N content in soils. The organic matter content in compost applied soils were negatively correlated with No value (r = - 0.69*). Therefore, our result indicated that determination of N application rate in livestock manure compost applied soil should be based on total nitrogen content better than soil organic matter content.
흰쥐에서 땃두릅 에탈올 추출물의 14일 반복경구토여에 의한 독성시험
권혁세,김대환,신현경,유창연,김명조,임정대,박재군,김진경,Kwon, Hyuck-Se,Kim, Dae-Hwan,Shin, Hyun-Kyung,Yu, Chang-Yeon,Kim, Myong-Jo,Lim, Jung-Dae,Park, Jae-Kun,Kim, Jin-Kyung 한국식품과학회 2007 한국식품과학회지 Vol.39 No.4
본 연구는 시험물질 국내산 땃두릅나무 추출물의 반복 경구투여에 의한 독성을 질적 ${\cdot}$ 양적으로 평가하기 위하여 식품의약안전청의 의약품 등의 독성시험기준에 따라 Sprague-Dawley 계열의 암 ${\cdot}$ 수 흰쥐에 시험 물질을 0, 500, 1,000 및 2,000 mg/kg의 용량으로 14일간 반복경구 투여한 후 사망률, 일반증상, 체중변화, 혈액 및 혈액생화학적 변화, 부검소견, 조직학적인 변화를 관찰하였다. 시험기간 중 암 ${\cdot}$ 수 모든 군에서 시험물질 투여에 기인한 일반적인 증상변화는 관찰되지 않았고, 시험불질의 반복 투여로 인한 사망례 역시 관찰되지 않았다. 시험물질의 투여에 기인한 유의적인 체중감소 또한 나타나지 않았으며, 상기 이외의 육안적인 부검소견에서도 시험물질 투여에 기인한 어떠한 이상소견도 발견되지 않았다. 혈액학적 분석 결과 일부 시험물질 투여군에서 WBC, MCHC, PLT의 수치가 유의적인 변화를 보였으나, 정상범위 내에서의 변화로 시험물질에 의한 독성이라고 판단하기는 어렵다. 혈청중의 ALP 및 뇨의 BUN의 유의적인 감소가 시험물질 투여군에서 관찰되었으나 정상 생리 범위 내에서의 변화로 시험 물질에 의한 독성이라 판단 하기는 어렵다. 간장과 신장의 조직학적인 관찰에서 시험물질 투여에 의한 변화는 관찰되지 않았다. 본시험결과는 흰쥐에서 땃두릅나무 에단올추출물의 14일 반복경구 투여는 2,000 mg/kg 이하의 용량에서 어떠한 독성영향도 유발하지 않았으며, 본 시험 조건하에서 최소치사량은 2,000 mg/kg 이상이라는 것으로 나타났다. 따라서 본 시험을 통하여 땃두릅나무 추출물은 투여가능 최대 용량에서도 독성이 없는 안전한 천연물이라는 것을 확인하였고, 의약품이나 기능성식품으로서의 개발 가능성을 확인하였다. Oplopanax elatus (O. elatus) is a tall deciduous shrub that has traditionally been used for σ eating a variety of ailments such as diabetes, coughling, rheumatism, gastro-intestinal disorders, and wounds. In order to examine the safety of the ethanol extracts of O. elatus, we performed a 14-day repeated-dose toxicity study with Sprague-Dawley rats. The rats were treated with daily doses of the D. elatus ethanol extracts by gavage at 0, 500, 1000, and 2000 mg/kg/day for 14 consecutive days. We recorded clinical signs of toxicity, body weight, hematology, organ weights, gross and histological changes in target organs, and clinical chemistry analysis data for all rats. There were no significant changes in body and organ weights during the experimental period. The hematological analysis and clinical blood chemistry data revealed no toxic effects from the O. elatus ethanol extracts. Pathologically, neither gross abnormalities nor histopathological changes were observed between the control and treated rats of both sexes. Collectively, these data suggest that the ethanol extracts of O. elatus have a high margin of safety.