인삼이 칼륨결핍랫트 장점막의 Na+,K+-ATPase 활성에 미치는 영향

이명희(Myong Hee Lee),김낙두(Nak Doo Kim) 대한약학회 1988 약학회지 Vol.32 No.1

We have studied the effect of fasting on Na+,K+-ATPase activities in the rat intestinal mucosa. Rats were fasted for 18-48 hr. Intestinal microsomal fraction was prepared by the method of Robinson and ATPase activities were determined by the modified method of Fiske and Subbarow. Na+,K+-ATPase activity was not changed after fasting for 18 and 24 hr but significantly decreased after fasting for 48 hr. Fasting over 18 to 48 hr period had no effect on the Mg++-ATPase. Thus, it may be concluded that 48 hr fasting has inhibitory effect on rat intestinal absorptive capabilities. In order to study the effect of Ginseng on the Na+,K+-ATPase activities of the small intestine in chronic K+-depleted rats, rats were fed K+-depleted diets for 3 weeks and Ginseng ethanol extracts were administered orally for 3 weeks concomitantly. ATPase activity was measured by the same method as fasting group. Na+,K+-ATPase activity in the K+-depleted diet group was increased and Ginseng ethanol extracts inhibited the increase of enzyme activity induced by K+-depleted diet. Thus, it may be suggested that increase in the intestinal Na+,K+-ATPase activity of chronic K+-depleted group may be due to the compensatory mechanism and administration of Ginseng with K+-depleted diet may be associated with inhibition of increase in the enzyme activity of the K+-depleted group due to the prevention of the K+ loss in the K+-depletion.

의사소통 보조공학기기 지원과 관세정책에 관한 연구

이명희(Myong Hee Lee),배내윤(Nae Yoon Bae) 한국무역연구원 2014 무역연구 Vol.10 No.6

This study was conducted to investigate the support of communication technology and tariff system, focused on the condition and needs about the communication of the people with cerebral palsy. For this, this study examined the condition and needs of communication about the people with cerebral palsy. And literature research was done about communication technology and tariff system. The results of this study were summarized as follows: Individuals with good communication is a little, they want communication support, almost of them do not know augmentative and alternative communication, they have the difficulties of communication in their social lives, they need communication technology support system. Based on research findings, this study suggested that the methods of support increase for communication technology for people with cerebral palsy, and that reduction of tariff system and the increase of export for communication technologies developmented by korean.

EMTP MODELS를 이용한 디지탈 거리계전기법의 구현에 관한 연구

李明熙(Myong-Hee Lee),徐容必(Yong-Pil Seo),崔亥述(Hae-Sul Choi),金喆煥(Chul-Hwan Kim),洪光錫(Kwang-Seok Hong),蔡永茂(Yong-Moo Chae) 대한전기학회 1996 전기학회논문지 Vol.45 No.11

EMTP MODELS를 사용한 거리계전기법 구현에 관한 연구

이명희(Myong-Hee Lee),최해술(Hae-Sul Choi),서용필(Yong-Pil Seo),김철환(Chul-Hwan Kim) 대한전기학회 1995 대한전기학회 학술대회 논문집 Vol.1995 No.7

Surface Activities of Ginseng Saponins and Their Interactions with Biomolecules(I) - Separations and Surface Activities of Major Saponins from Fresh Ginseng Roots

이상직,이명희,이강자,Lee, Sang-Jik,Lee, Myong-Hee,Lee, Kang-Ja 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.1

한국산 수삼의 부탄올 가용성 분획으로부터 규산을 흡착제로 사용한 column 및 thin-layer 크로마토그라피에 의하여 4종의 주성분 사포닌을 분리정제하고 모세관상승법으로 그들 수용액의 표면장력을 측정함으로써 그들의 계면활성의 면모를 평가하였다. 물의 표면장력은 이들 정세사포닌에 의하여 실온에서 약 50 erg/$cm^2$까지 내려가며, 사포닌의 종류에 따라 0.05 내지 0.2%에서 최소치를 기록하였다. 임계미셀농도는 농도-표면장력 곡선의 꺾임점으로부터 추정하였는데, 각각 0.05, 0.05, 0.2 및 0.2% 이었다. 이 값은 사포닌의 분자량을 1,000으로 보았을 때 0.5, 0.5, 2 및 2mM에 해당한다. 상기한 부탄올 가용성 분획은 사포닌 외의 물질이 거의 함유되지 아니하여 총사포닌 분획으로서 바람직하며, 개개의 사포닌을 정제함에 유용한 것으로 사료된다. Saponins of fresh ginseng roots were purified by repeated silicic acid column and thin-layer chromatographies from butanolextractable fraction of the roots. Surface activities of the purified saponins were characterized by measuring surface tensions of their aqueous solutions employing capillary-rise method. It was observed that surface tension of water was lowered down to ca. 50 erg/$cm^2$ at room temperature by these saponins and reached almost minima at the concentrations ranging from 0.05 to 0.2% saponins. Critical micellar concentrations approximated from the reflection points on the surface tension curves were 0.05, 0.05, 0.2, and 0.2% respectively, which correspond to 0.5, 0.5, 2 and 2mM if the molecular weights of the saponins were assumed to be 1,000. It was also proved that butanol-extractable fraction obtained from fresh ginseng in this study could be served as a desirable saponin mixture with little impurities and as a good source for saponin purifications. Fresh ginseng roots were found to contain four major saponins in their butanol extract.

인삼사포닌의 계면활성 및 생체분자와의 작용 ( 1 ) 수삼의 주사포닌의 분리와 계면활성

이상직,이명희,이강자 ( Sang Jik Lee,Myong Hee Lee,Kang Ja Lee ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.1

Saponins of fresh ginseng roots were purified by repeated silicic acid column and thin-layer chromatographies from butanolextractable fraction of the roots. Surface activities of the purified saponins were characterized by measuring surface tensions of their aqueous solutions employing capillary-rise method. It was observed that surface tension cf water was lowered down to ca. 50 erg/㎠ at room temperature by these saponins and reached almost minima at the concentrations ranging from 0.05 to 0.2% saponins. Critical micellar concentrations approximated from the reflection points on the surface tension curves were 0.05, 0.05, 0.2, and 0.2% respectively, which correspond to 0.5, 0.5, 2 and 2mM if the molecular weights of the saponins were assumed to be 1,000. It was also proved that butanol-extractable fraction obtained from fresh ginseng in this study could be served as a desirable saponin mixture with little impurities and as a good source for saponin purifications. Fresh ginseng roots were found to contain four major saponins in their butanol extract.

도.토기의 과학적 분석 및 분류-전남 완도군 장도 청해진 유적을 중심으로

홍종욱,조남철,강대일,이명희,Hong, Jong-Ouk,Cho, Nam-Chu,Kang, Dai-Il,Lee, Myong-Hee 국립문화재연구소 2000 保存科學硏究 Vol.21 No.-

This study has proved if there are homogeneities in crystal structures, firing temperaures and element compositions of 69 specimens for potteries and soils from Changdo, Kangjin-gun, Jinjuk-Ri in Korea and Yaezhou inChina and obtained the following results.1. XRD analyses showed that soft potteries seemed to contain ($\alpha$)quartz, feldspar, while hard potteries seemed to contain ($\alpha$-)quartz, mullite, tridymite, feldspar. Jinjuk-Ri soils consisted of clays such as Kiolinite, Montmorillonite, Muscovite, Illite and, mica and feldspar etc.2. It was estimated that the firing temperatures which are determined by crystals using XRD, ranged from $550^{\circ}C$ to $870^{\circ}C$ for soft potteries and $870^{\circ}C$ to $975^{\circ}C$ for hard potteries.3. The firing temperatures for 4-pieces of Changdo pottery were measured by using TG-DTA, and it was found that specimen No. 10 was fired at temperatures below $600^{\circ}C$, while specimens No. 14, No. 23 and No. 29 were fired at temperatures above $1000^{\circ}C$.4. It was found that the specimems for potteries and porcelains from Changdo, Kangjin-gun, Jinjuk-Ri in Korea and Yaezhou in China were apparently classified into 4 clusters. This suggests that there are no correlations between the raw materials used in each 4 regions. Among the porcelains from Changdo, there were many typologically similar ones to those from Yaezhou in China in the 9th centry, but the analyses of porcelains only from Changdo, Kangjin in Korea and Yaezhouin China showed that they were classified into 3 clusters. This suggest that there are no correlations between the raw materials used for making porcelains in Changdo, Kangjin-gun, Jinjuk-Ri in Korea and Yaezhou in China.

석조문화재 발수경화제 시험 연구(I)

엄두성,김사덕,홍정기,강대일,이명희,Eom, Doo-Sung,Kim, Sa-Dug,Hong, Jung-Ki,Kang, Dai-Il,Lee, Myong-Hee 국립문화재연구소 1999 保存科學硏究 Vol.20 No.-

We studied to select the best consolidant and water-repellentsynthesis resins on the weathering caused by many factors(temperature variation, ultraviolet light, moisture, salt etc.) for using internal or external of stone cultural properties. The specimen〔granite(Pochon-suk), granite(Hwangdung-suk), tuff(in Kyong-ju)〕was treated by Wacker OH, Wacker OH 100, Wacker 290L, D.W.R, SS-101and tested in the five kinds of test and analyzed. After all test, D.W.R is the excellent results on the measuring of the property of matter. Especially, water-repellent efficiency is the best.

석조문화재 받침용 쐐기 재질의 물성 실험 연구

엄두성,홍정기,김사덕,강대일,이명희,Eom, Doo-Sung,Hong, Jung-Ki,Kim, Sa-Dug,Kang, Dai-Il,Lee, Myong-Hee 국립문화재연구소 2000 保存科學硏究 Vol.21 No.-

Stone cultural properties(pagoda, Buddhist statue etc.) is moved in original place for many reasons and restored after taking to pieces for structural safety. With the movement and restoration after taking to pieces, the cast iron is used for the sake of a fixation and horizontality. The stone cultural properties are spoiled the beauty and accelerated the weathering because of the corrosion product of cast iron. So we need to substitute for the improved property in all aspects. We are executed the corrosion test and inquired the property of material on the usable material for the shim of stone cultural properties. That is the Cast iron, Stainless steel, Titanium and Fiber Reinforced Plastics. In the result of the physical property and strength, the Stainless steel and Titanium was superior to the Cast iron. And the Stainless steel and Titanium was slower than the Cast iron in the corrosion velocity for the acid, salt and rain. If the shim is substitute the Stainless steel or Titanium for the Cast iron, the stone cultural properties are able to reduce the pollution of stone cultural properties by corrosion product and should not happen in the matter of stone pagoda.

출토 인골의 유전자분석-나주 복암리 3호분 옹관 인골을 중심으로

이규식,정용재,한성희,이명희,한면수,최동호,Lee, Kyu-Sik,Chung, Yong-Jae,Han, Sung-Hee,Lee, Myong-Hee,Han, Myun-Soo,Choi, Dong-Ho 국립문화재연구소 1999 保存科學硏究 Vol.20 No.-

We have analyzed the allele and genotype frequencies from 10 fractions of ancient human skeleton in 3 pieces of Jar coffin excavated from Naju Bokamni3rd tumulus by PCR amplification, high resolution polyacrylamide gelelectorphoresis and silver staining. We could isolate human genomic DNA from 3 bone fractions but the rest of them could not be used as materials due to being decayed. We could detect sex determination as male and 3 genotypes of STR system, HUMTHO1, HUMTPOX and HUMC5F1PO from the bone fraction of left side in Jar coffin 3 and see the slightly reaction suggesting the sex as male from the bone fraction of the left side in Jar coffin 2 and female from the right side in Jar coffin 3.We have also analyzed the genotype frequencies of mitochondria from the bone fractions of the left side and the right side in jar coffin 3, respectively. From the result of indetifiying at nucletide position between 16018 and 16378of the base of hyper variable region(HV1) in the control region, We can presume that the both bones have the same maternal inheritance.