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항균소재를 함유시킨 저밀도폴리에틸렌 필름에 의한 상추와 오이의 포장
안덕순(Duck-Soon An),황용일(Yong-II Hwang),조성환(Sung-Hwan Cho),이동선(Dong-Sun Lee) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.4
원예산물의 미생물적 변패를 억제할 수 있는 포장필름을 개발하기 위하여, 대황추출물, 황련추출물, sorbic acid, 지르코니움계 은치환무기이온교환체와 같은 몇가지 항균성 소재를 저밀도폴리에틸렌에 1% 농도로 첨가하여 필름으로 제조한 다음, 이들 필름의 물성을 비교하고 항균성을 몇가지 변패미생물에 대해 평판배지상에서 항균성을 검사하였다. 제조된 필름을 이용하여 상추와 오이를 포장하고 각각 5℃ 및 10℃에서 저장하면서 총균수 및 물리화학적 품질변화를 측정하였다. 대황추출물, 황련추출물, 지르코니움계 은치환무기이온교환체를 함유시킨 필름들은 공시된 Escherichia coli, Staphylococcus aureus, Leuconostoc mesenteroides, Saccharomyces cerevisiae, Aspergillus niger, Aspergillus oryzae, Penicilluium chrysogenum 등의 균주에 대해서 생육저해성을 나타내지 않았으나, sorbic acid를 함유시킨 필름은 평판배지상에서 E. coli, S. aureus, L. mesenteroides에 대해서는 항균활성을 보였다. 필름에 1% 농도로 첨가된 항균성 소재는 필름에 색택을 부여하여 약간의 광선투과도를 감소시키는 것 외에는 기계적 강도와 열수축성, 방담성의 측면에서 필름의 물성에 부정적인 영향을 주지 않으며 일부 측면에서는 오히려 긍정적인 효과를 보였다. 항균성 소재를 첨가시킨 포장필름으로 상추와 오이를 포장하였을 때에는, 총균수가 10^8/g의 범주에 이르기 전에는 미생물의 증식을 억제하는 효과를 보이면서 이들 채소의 다른 품질에 부정적인 영향을 주지 않았다. Low density polyethylene(LDPE) films of 50㎛ thickness were fabricated with addition of antimicrobial agents of Rheum palmatum extract, Coptis chinensis extract, sorbic acid and Ag-substituted inorganic zirconium matrix in 1% concentration. The films were compared in physical properties, tested in antimicrobial activity against some selected microorganisms on the agar plate medium and then applied for packaging fresh curled lettuce and cucumber to preserve their qualities. The films with Rheum palmatum extract, Coptis chinensis extract, and Ag-substituted inorganic zirconium matrix did not show any antimicrobial activity on the disk test against Escherichia coli, Staphylococcus aureus, Leuconostoc mesenteroides, Saccharomyces cerevisiae, Aspergillus niger, Aspergillus oryzae, Penicilluium chrysogenum, while film with sorbic acid did against E. coli, S. aureus and L. mesenteroides. The added antimicrobial agents changed the color and light transmittance of the films, but did not affect their mechanical tensile strength, heat shrinkage and wettability. For the packaged curled lettuce and cucumber stored at 5℃ and 10℃, all the LDPE films impregnated with antimicrobial agents showed the reduced growth of total aerobic bacteria in the vegetables compared with control film without any additive until it reached the level around 10^8/g. They did not give any negative effect on other quality attributes during storage.
원위기저 후비복 도서형 근막피판을 이용한 하지 연부조직결손의 재건
황일면,김용배,강상규,양순재,박종섭 大韓成形外科學會 1995 Archives of Plastic Surgery Vol.22 No.4
The covering of extensive soft-tissue defects over the foot, heel, and ankle remains a common and difficult clinical entity. In general, soft tissue of the extremities is more plentiful in the proximal than the distal part. It is therefore, not difficult to obtain adequate tissue to reconstruct soft tissue defects of the upper or middle third of the leg. However, in the lower part of the leg, a proximally based fasciocutaneous flap cannot be easily used as adequate donor tissue for reconstruction because the soft tissue near the ankle is relatively tight and scanty. On the contrary, if the flap is based distally, we can extend the flap toward the proximal part of the leg, where more soft tissue can be harvested for reconstruction. The principle of fasciocutaneous flap was clearly described by Ponten in 1981¹. In 1983 Donski and Fogdestam described a distally based fasciocutaneous flap from the sural region based on perforating branches from the peroneal artery and in 1986², Amarante et al.reported a distally based fasciocutaneous flap based on the perforators from the posterior tibial vessels³. With further detailed knowledge of the circulation of skin of the leg, the distally based posterior calf fasciocutaneous island flap has been used recently and excellent results in reconstruction of soft tissue defects in the lower one third of the extremities have been obtained. In this paper, a distally based posterior calf fasciocutaneous island flap, nourished by the lower most perforator originating from the posterior tibial at about 4-6.5cm above the medial malleolus and peroneal artery at about 5cm above the lateral melleolus, has been used success fully for reconstruction of the lower leg, ankle, and foot. The advantages are as follows: the blood supply is reliable, elevation is easy and quick, and major arteries are not sacrificed.
Aspergillus niger KCTC6134에 의한 phytase생산에 미치는 탄소원과 질산원의 영향
이승철,황용일,이대희 Institute of Genetic Engineering Changwon National 1997 Gene and Protein Vol.1 No.1
In order to maximize the production of phytase from Aspergillus niger KCTC6134, souse of factors influencing both phytase production and mycelial growth were investigated in shake-flask cultures. The optimal production condition of phytase by each concentration of the optimal carbon and nitrogen sources was examined. The optimal production condition of phytase was obtained with 10% soluble starch 3% glucose. and 0.8% KNO₃at 28℃. The time course of phytase production showed 5.0-fold increase, which correspond to the maximum of a mycelial growth and compare with the basal medium used.