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톱밥 발효돈사(醱酵豚舍) 사육돈(飼育豚)의 내부기생충(內部寄生蟲) 조사(調査)
이병훈 ( Byung Hoon Lee ),황보원 ( Bo Won Hwang ),변유성 ( Yu Seong Byun ),이순선 ( Soon Sun Lee ),김차용 ( Cha Yong Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2
This study was performed to survey on the prevalence of swine internal parasites at 12 piggeries being operated by the sawdust fermentative pigsty in Kyongnam central area. To investigate the effects of viability of eggs, larva and cysts, temperatures at the underlayer of the sawdust floor and rooms of pens were taken four times each season for one year. In a pig farms which have been operated by the sawdust fermentative pigsty, eggs of Ascar is suum(8.8%), Trichur is suis(18.3%), Oesophagostomum sp(9.4%), Strongyloides ransomi(1.3%), Hyostrongylus sp(3.8%) and Metastrongylus apri(2.3%) were detected from 480 heads of pigs. Cysts and oocysts of Coccidia(12.7%), Balantidium coli(5.4%) were also detected form 480 heads of pigs. The change of the temperature in the pig room was variable between 13℃ to 27℃ according to outside condition, whereas that in underfloor was consistently stable between 28℃ to 31℃ which was strictly suitable for the development of eggs, larva and cysts.
돼지 분만 시기에 조절에 관하여: 3. 자궁 평활근의 운동성에 대한 Histamine의 영향
박상은 ( Sang Eun Park ),황보원 ( Bo Won Hwang ),변유성 ( Ye Seong Byun ),조광제 ( Kwang Je Cho ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2
The effects of histamine were investigated on the uterine smooth muscle motility in the pig. The results were summarized as fellows: 1. Histamine caused the contraction of the porcine uterine smooth muscle and the contractile responses increased between the concetration of histamine 10(-8) and 10(-5) M with a dose-dependent manner. 2. The contractile response induced by histamine(10(-6) M) was completely blocked by pretreatment with H1-histaminergic receptor blocker, pyrilamine(10(-6) M). 3. The contractile response induced by histamine(10(-6) M) was increased by pretreatment with H2-histaminergic receptor blocker, cimetidine(10(-6) M). From these results, it was concluded that the effects of uterine smooth muscle by histamine were the contraction mediated by H1-histaminergic receptor and the relaxation mediated by H2-histaminergic receptor in pig.
Real-time PCR을 이용한 돼지써코바이러스 감염증 진단법 연구
김은경 ( Eun Gyeong Kim ),황보원 ( Bo Won Hwang ),이종민 ( Jong Min Lee ),손병국 ( Byeong Guk Son ),박호정 ( Ho Jung Park ),김도경 ( Tho Kyoung Kim ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.4
Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the Real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied Real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR, becoming increasingly important method for gene analysis.
식육 중 항균물질(플루오르퀴놀론계) 동시 다성분분석법 개선 연구
박동엽 ( Dong Yeob Park ),황보원 ( Bo Won Hwang ),조성숙 ( Sung Suk Cho ),최찬영 ( Chan Young Choi ),조상래 ( Sang Lae Cho ),박애라 ( Ae Ra Park ),정은희 ( Eun Hee Jung ),변유성 ( You Sung Byun ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.2
A direct, accurate and sensitive chromatographic analytical method for quantitative determination of four fluoroquinolones(norfloxacin, cirprofloxacin, danofloxacin and enrofloxacin) in chicken, pork and beef edible muscle is proposed in the present study. The developed method was successfully applied to the determination of enrofloxacin, as the main component of commercially available veterinary drugs. The samples were homogenized and the antimicrobials were added, then they were extracted twice with dichloromethane. Fluoroquinolone antibiotics were separated on an agilent 250×4㎜, C18, 5㎛, analytical column, at 25℃. The mobile phase consisted of a mixture of DW:acetonitrile:triethylamine(80:19:1%, v/v, pH 3.0) leading to retention times less than 14min. at a flow rate 0.5㎖/min. These fluoroquinolones were detected by liquid chromatography with fluorescence at 290㎚ excitation and 465㎚ emission. The limits of quantification in each edible muscle(chicken, pork, and beef) were 0.32-6.54ng/g. Using 0.5g of each sample, average recovery rates at fortification levels of 0.05, 0.1 and 0.2㎍/㎖ ranged 70.14-71.71% for NFX, 71.87-73.89% for CFX, 82.16-92.35% for DFX, and 90.13-98.12 for EFX. This is a simple and economic method to quantify the presence of NFX, CFX, EFX and DFX in edible muscle of animal origin.
돼지 분만 시기의 조절에 관하여: 4. 자궁 평활근의 운동성에 대한 APT의 영향
박상은 ( Sang Eun Park ),황보원 ( Bo Won Hwang ),변유성 ( Yu Seong Byuyu ),조광제 ( Kwang Je Cho ) 한국가축위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.2
The effcets of adenosine 5`-triphosphate(ATP) were investigated on the uterine smooth muscle motility in the pig. The results were summarized as follows: 1. The effects of the porcine uterine smooth muscle and the contractile responses increased between the concentration of ATP 10(-5) and 10(-3)M with a dose-dependent manner. 2. The contractile response induced by ATP(10(-4) M) was not blocked by pretreatment with cholinergic receptor blocker, atropine (10(-6) M). 3. The contractile response induced by ATP (10(-4) M) was not blocked by pretreatment with α-adrenergic receptor blocker, phentolamine (10(-6) M) and β-adrenergic blocker, propranolol (10(-6) M). 4. The contractile response induced by ATP(10(-4) M) was not appeared in Ca(++)-free medium. As the concentration of Ca++ in Ca(++)-free medium was increased, the contractile response induced by ATP (10(-4) M) was enhenced but was completely inhibited by pretreatment with Ca(++)-channel blocker, papaverine(10(-6) M) or verapami1(10(-6) M). From these results, it was conclued that the effects of ATP were the contraction mediated by purinergic receptor in uterine smooth muscle of pig.
도계처리 단계별 도체와 처리수의 세균오염 및 염소처리 효과
이철현 ( Chul Hyun Yi ),변유성 ( You Sung Byun ),황보원 ( Bo Won Hwang ),조광제 ( Kwang Je Cho ),강호조 ( Ho Jo Kang ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2
This study was carried out assess the effect of the chlorine treatment into water for processing chicken products in each stage of slaughtering, with a special viewpoint related reducing the viable number of microorganisms by which the water and the chicken body were contaminated. The mean bacterial number on chicken samples after picking process was log5.37±0.20~5.84±0.16CFU/㎠. When assessed by standard plate count method, it was the higher one than any other processing stage in which eviscerating, pinning, packaging, and chilling was followed in order of the mean bacterial number. The coliform bacterial numbers on carcasses after sampling from different processing stages were log2.11±0.63~2.88±0.25MPN㎠, which show almost similar numbers in each processing stage. But, after chilling process the number was decreased slightly. The bacterial counts in the water for scalding and chilling showed log3.43±0.59~5.06±0.21 and log 4.30±0.21~6.62±0.33CFU/ml, respectively. In the coliform counts for the water taken out from the 2nd chilling tank, the number was log1.97±0.35~2.91±0.22MPN/ml which showed higher than those of the 1st and the 3rd chilling tank water. The effect of chlorination in reducing the bacterial numbers was accepted at the residual chlorine concentration of 1mg/l by showing the reduction from 108 to 104CFU level and the numbers were decreased less than 10CFU at the concentration of 5mg/l, when assessed by viable cell counts. In conclusion, these results suggested that chlorination in chilling water with final concentration of 5mg/l was strongly recommended to reduce the bacterial numbers on final chicken products.
경남지역에서 도축우 및 돼지의 근육내 잔류항균물질 검색
박동엽 ( Dong Yeob Park ),양평섭 ( Pyeong Seob Yang ),남창우 ( Chang Woo Nam ),황보원 ( Bo Won Hwang ),김원규 ( Won Gyu Kim ),조상래 ( Sang Rae Jo ),김도경 ( Toh Kyung Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
The present studies were carried out to determine antibiotics residues in pork and beef muscles by EEC-4-plate and HPLC. A total of 2,534 samples of pork muscles and 1,070 samples of beef muscles from slaughter houses were collected in Gyeongnam area from January to December, 2001. The results were summarized as follows; 1. Recovery rates of TCs, Sulfa drug, Penicillin G from fortified pork and beef muscles ranged as 68.79~98.24%, 78.21~94.58% and penicillin G 63.35~67.24% respectively, by HPLC. 2. Antibiotics residues were detected in 36 sample(1.42%) of pork muscles, 29 sample (2.71%) of beef muscles by EEC-4-plate. 3. Detection rate of antibiotic residues 14 samples(0.55%) and 26 samples(2.43%), in pork and beef muscles, respectively by HPLC. Concentration of residues in 22 sample(2.06%) of beef muscle were higher than tolerance level in korea. 4. Antibiotics detected were sulfamethazine(47.37%), tetracycline(15.79%), oxytetracycline(15.79%), penicillin G(15.79%), sulfamerazine(5.26%) in pork muscle samples and oxyteracycline(37.21%), penicillin G(30.23%), sulfamethazine(20.93%), tetracycline(4.65%), sulfamerazine(2.33%), sulfadimethoxine(2.33%), sulfaquinoxine(2.33%) in beef muscle samples.
닭고기에서 병원성 및 변질미생물의 감소를 위한 염소와 유산의 병용처리 효과
이철현 ( Chul Hyun Yi ),변유성 ( You Sung Byun ),황보원 ( Bo Won Hwang ),강호조 ( Ho Jo Kang ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.4
In this studies, the ability of chlorine and lactic acid to reduce bacterial population of the pathogenic microorganisms were examined on artificially inoculated chicken skin. About 10(5) cells of staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and escherichia coli O157:H7 were inoculated in chicken skin. The contaminated samples were washed for 1 min with sodium hypochlorite solutions that contained 2, 5, 10, 20 and 50mg/ℓ available chlorine and counted number of the agents. Viable population were no significantly difference (p ≥ 0.05) between concentration of chlorine and strains of the pathogens. In the samples inoculated with pathogens were washed in 20mg/ℓ chlorine and then stored at 5°C for up to 10 days, the initial counts of psychrotrophs and aerobic plate counts were 4.02 to 4.36 log cfu/cm2 and increased slightly in course of time. But 10 days after, the pathogens were a little reduced from 3.66~4.91 log cfu/cm2 to 2.54~4.66 log cfu/cm2. In the case of washed skin with solution of 20mg/ℓ chorine and 0.5% lactic acid then store at 5°C for up to 10 days, population of psychrotrophs and aerobic plate counts on chicken skin were markedly reduced immediately after treatment, but the numbers of contaminants were slightly increased after 6 and 8 days. Specifically, numbers of St aureus, S enteritidis, L monocytogenes and E coli 0157:H7 were reduced to 0.5, 0.4, 0.3 and 1.15 log cfu/cm2 after 10 days of storage, respectively, on aerobic plate counts.
인공수정용 돼지 액상정액 세균오염도 조사 및 정액유래 주요 바이러스성 질병 감염률 조사
손병국 ( Byeong Guk Son ),박호정 ( Ho Jung Park ),김은경 ( Eun Gyeong Kim ),이종민 ( Jong Min Lee ),황보원 ( Bo Won Hwang ),허정호 ( Jung Ho Heo ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.4
Bacteroiospermia is a frequently finding in fresh raw and extended porcine semen and can results in detrimental effects on semen quality and longevity. This study aims to evaluate the type of bacterial contaminants in raw and extended porcine semen and the reducing effect of antibiotic test. To investigate bacterial contaminants, out of 387 sample (raw semen 201, extended semen 186) were collected from 6 artifical insemination centers in Gyeongsangnam-do, were inoculated onto blood agar and MacKonkey agar, respectively. Bacterial colonies were selected after culturing for 48 hours, at 37℃, followed by Gram staining, KOH test, oxidase test, catalase test and eventually identified using VITEK System. Total 15 genus and 24 species of bacteria were isolated from these semen samlpes. In raw semen, the most prevalent contaminants were Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis, Staphylococcus auricularis, Delftia acidovorans, Acinetobacter lowffii, S. aureus and others. And in extended porcine semen, A. lowffii, S. aureus, S. auricularis and other bacteria were identified. Most of them was G (-), which is nonpathogenic bacteria. It seems that bacterial contaminants in fresh raw and extended porcine semen originated from multiple sources at the farms/stud, and were from animal origin and non-animal origins. Whereas, the 7 virus which is known to be detected in porcine semen in 75 cases was not detected. This results showed that removal of bacterial contamination in raw and extended porcine semen is essential and farms were kept for biosecurity and individual hygienes.